This document provides information on cultivating bacteria, including definitions, purposes, and requirements. It discusses cultivating bacteria in vitro for identification, antibiotic susceptibility testing, industrial uses, and research. The key requirements for in vitro cultivation are nutritional needs provided by culture media, temperature, gaseous atmosphere, and humidity. Various types of culture media are described based on consistency, oxygen requirements, and constituents. Selective, enriched, differential, and transport media are among the special types discussed.

1. Cultivation of bacteria
Almugadam B Saad
Lecturer of medical microbiology
Faculty of medical laboratory sciences Full name
Babiker Saad Almugadam Yousif
Faculty of medical laboratory sciences
University of El Imam El Mahadi
Kosti-Sudan
MSc University of Khartoum
BSc University of El Imam Al Mahadi
Babiker Saad Almugadam Yousif

2. Cultivation of bacteria
- Definition of cultivation
- Purpose of cultivation
- Requirements of cultivation
Learning
objectives
- Requirements of cultivation
- Culture media and their Types

3. Cultivation
- Definition : process of propagation of microorganism.
Can In vivo or
In vitro
- Purposes from bacterial cultivation :
1. To identify bacteria 2. AST
3. Industrial 4. Genetic engineering and research
- Requirements of invitro cultivation :
A. Nutritional: which provide by using culture medium
B. Temperature
C. Gasses atmosphere
D. Humidity

4. culture medium
- Definition : Medium use to cultivate micro-organism , it provide
nutrients and optimum pH for growth of micro-organism.
- Components of culture media : Water , agar , and …….
- Types of culture media :
Liquid (Fluid) media
A. base on consistency : Semisold (Semifluid) mediaA. base on consistency : Semisold (Semifluid) media
Sold media
Aerobic media
B. base on oxygen requirement : Anaerobic media
Simple
C. base on constituents (ingredients) : Complex
Synthetic (Defined)
Special

5. A. base on consistency :
1. Liquid (Fluid) media : Fluid , Not contain agar
Example : Peptone water
2. Semisold (Semi fluid) media : Semi fluid , contain 0.5g of agar
Example : Amies transport media
3. Sold media : sold , contain 1g or more of agar
Example : Nutrient agarExample : Nutrient agar
B. base on oxygen requirement :
1. Aerobic media: Use to grow anaerobic bacteria
2. Anaerobic media : Use to grow anaerobic bacteria , because it
contain substance provide anaerobic conditon.
Example : Robertous cooked meat media , and Thioglycolate
broth.

6. C. base on constituents (ingredients) :
A. Simple (basal media) : contain simple (basal) ingredients ,
use for culture of Non fastidious bacteria , Can be sold or fluid.
Example : Nutrient agar , Peptone water
B. Complex : contain added ingredients to provide special nutrient
C. Synthetic (Defined) : synthesize from pure chemical substances
and their exact composition is known.and their exact composition is known.
D. Special : Classify according to function for
1. Selective 2. Enriched
3. Enriched 4. Differential
5. Indicator ? 6. Transport ?
7. Biochemical's ?

7. 1. Selective : contain inhibitory material(selective agent) to suppress
growth of un wanted bacteria.
Selective agent : material use to inhibit growth of certain bacteria,
Can be Antibiotics , Dyes , or chemicals.
Example : MacConkey agar for enterobacteriace family
2. Enriched : it sold media enrich with nutrients like blood or serum
,use for culture of fastidious bacteria.
Example : Blood agar
3. Enrichment : it liquid media enrich with nutrients and contain
selective agent ,use to isolate bacteria contaminated sample.
Example : SF broth
4. Differential : Has substance incorporate in it , enable it to
distinguish between bacteria .
Example : XLD medium