This document describes a study using a miniature mass spectrometer to rapidly discriminate between bacterial species based on their unique phospholipid profiles obtained through paper spray ionization. Mass spectra from eight bacterial species were analyzed and differentiated using multivariate analysis. The miniature mass spectrometer was able to produce phospholipid profiles and tandem mass spectra that were comparable to a benchtop linear ion trap mass spectrometer, demonstrating its capability to analyze meso-size biomolecules like phospholipids for bacterial discrimination. While there was some day-to-day variability, significant differences were observed between the lipid profiles of several bacterial species, showing potential for portable in situ analysis of microorganisms.
The use of agrochemicals has increased considerably in recent years, and consequently, there has been increased exposure of ecosystems and human populations to these highly toxic compounds. The study and development of methodologies to detect these substances with greater sensitivity has become extremely relevant. This article describes, for the first time, the use of atomic force spectroscopy (AFS) in the detection of enzyme-inhibiting herbicides. A nanobiosensor based on an atomic force microscopy (AFM) tip functionalised with the acetolactate synthase (ALS) enzyme was developed and characterised. The herbicide metsulfuron-methyl, an ALS inhibitor, was successfully detected through the acquisition of force curves using this biosensor. The adhesion force values were considerably higher when the biosensor was used. An increase of ~250% was achieved relative to the adhesion force using an unfunctionalised AFM tip. This considerable increase was the result of a specific interaction between the enzyme and the herbicide, which was primarily responsible for the efficiency of the nanobiosensor. These results indicate that this methodology is promising for the detection of herbicides, pesticides, and other environmental contaminants.
2019 - Profiling of filamentous bacteria in activated sludge by 16s RNA ampli...WALEBUBLÉ
Abstract: In this study, filamentous bacteria in the activated sludge of a WWTP were investigated throughout a one-year period using high-throughput short-read (Illumina) and full-length (PacBio) 16S rRNA gene amplicon sequencing. The results showed that a total of 28 filamentous bacteria genera
were identified using Illumina sequencing. Also, we found 25 species using PacBio sequencing, belonging to Curvibacter, Mycobacterium, Haliscomenobacter, Defluvicoccus, Sphaerotilus, Thiothrix, Leptothrix, Gordonia and Tetrasphaera genera. Active Volatile Suspended Solids (AVSS) were
calculated from ATP data contained in living microorganisms, this parameter represents the living biomass concentration, and the food/microorganisms ratio (F/M ratio) was calculated using AVSS instead of MLVSS. To assess the contribution of the F/M ratio to the variability observed in the filamentous bacteria structure we carried out distance-based linear models (DISTLM) and distancebased redundancy analysis (dbRDA).
2010 - Assessment of plausible bioindicators for plant performance in advance...WALEBUBLÉ
Reference
Pérez-Uz, B., Arregui, L., Calvo P., Salvadó H., Fernandez N., Rodríguez E., Zornoza, A., Serrano, S. (2010) Assessment of plausible bioindicators for plant performance in advanced wastewater treatment. Water Research 44: 5059-5069.
2017 - Comparison of nitrifying microbial communities of two full-scale membr...WALEBUBLÉ
Barbarroja, P., Moreno-Mesonero, L., Zornoza, A., Fernández-Navarro, J., Alonso, J.L., Muñagorri, F., García, C., Álvarez, C. (2017) Comparison of nitrifying microbial communities of two full-scale membrane bioreactors treating wastewaters from municipal solid wastes using 16S rDNA gene amplicon sequencing. 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
The use of agrochemicals has increased considerably in recent years, and consequently, there has been increased exposure of ecosystems and human populations to these highly toxic compounds. The study and development of methodologies to detect these substances with greater sensitivity has become extremely relevant. This article describes, for the first time, the use of atomic force spectroscopy (AFS) in the detection of enzyme-inhibiting herbicides. A nanobiosensor based on an atomic force microscopy (AFM) tip functionalised with the acetolactate synthase (ALS) enzyme was developed and characterised. The herbicide metsulfuron-methyl, an ALS inhibitor, was successfully detected through the acquisition of force curves using this biosensor. The adhesion force values were considerably higher when the biosensor was used. An increase of ~250% was achieved relative to the adhesion force using an unfunctionalised AFM tip. This considerable increase was the result of a specific interaction between the enzyme and the herbicide, which was primarily responsible for the efficiency of the nanobiosensor. These results indicate that this methodology is promising for the detection of herbicides, pesticides, and other environmental contaminants.
2019 - Profiling of filamentous bacteria in activated sludge by 16s RNA ampli...WALEBUBLÉ
Abstract: In this study, filamentous bacteria in the activated sludge of a WWTP were investigated throughout a one-year period using high-throughput short-read (Illumina) and full-length (PacBio) 16S rRNA gene amplicon sequencing. The results showed that a total of 28 filamentous bacteria genera
were identified using Illumina sequencing. Also, we found 25 species using PacBio sequencing, belonging to Curvibacter, Mycobacterium, Haliscomenobacter, Defluvicoccus, Sphaerotilus, Thiothrix, Leptothrix, Gordonia and Tetrasphaera genera. Active Volatile Suspended Solids (AVSS) were
calculated from ATP data contained in living microorganisms, this parameter represents the living biomass concentration, and the food/microorganisms ratio (F/M ratio) was calculated using AVSS instead of MLVSS. To assess the contribution of the F/M ratio to the variability observed in the filamentous bacteria structure we carried out distance-based linear models (DISTLM) and distancebased redundancy analysis (dbRDA).
2010 - Assessment of plausible bioindicators for plant performance in advance...WALEBUBLÉ
Reference
Pérez-Uz, B., Arregui, L., Calvo P., Salvadó H., Fernandez N., Rodríguez E., Zornoza, A., Serrano, S. (2010) Assessment of plausible bioindicators for plant performance in advanced wastewater treatment. Water Research 44: 5059-5069.
2017 - Comparison of nitrifying microbial communities of two full-scale membr...WALEBUBLÉ
Barbarroja, P., Moreno-Mesonero, L., Zornoza, A., Fernández-Navarro, J., Alonso, J.L., Muñagorri, F., García, C., Álvarez, C. (2017) Comparison of nitrifying microbial communities of two full-scale membrane bioreactors treating wastewaters from municipal solid wastes using 16S rDNA gene amplicon sequencing. 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
2017 - Environmental Ordination of Filamentous Bacteria in Activated SludgeWALEBUBLÉ
Reference:
Zornoza, A., Serrano, S. and Alonso, J.L. (2017) Environmental Ordination of Filamentous Bacteria in Activated Sludge. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
2017 - Plausible Bioindicators of Biological Nitrogen Removal Process in WWTPsWALEBUBLÉ
Reference:
Zornoza, A., Alonso, J.L. and Serrano, S. (2017) Plausible Bioindicators of Biological Nitrogen Removal Process in WWTPs. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
Modelling the Kinetic of UV Water DisinfectionMichael George
Ultraviolet disinfection is an attractive tool for treating water and eliminating pathogens with safe and available technology especially in developing countries where waterborne diseases cause the death of thousands of people every year. Even though UV is an easy tool to perform disinfection, concerns over the potential of microorganism reactivation constitute an issue for its development. In order to avoid this phenomenon, estimating the right dose of UV irradiance, the number of viable microorganisms and the sufficient contact time are important parameters to consider when performing UV disinfection. For this purpose, it’s current to use mathematical modelling. This work aimed to study the modelling of the kinetic of water disinfection by UV irradiation. Two kinetic models (Chick-Watson and Hom) were tested as to ability to scale disinfection of Gram negative Escherichia coli and Gram positive Lactobacillus Helveticus by different ultraviolet (UV) light inactivation process: UV alone, UV and TiO2 as a photocatalyst and finally UV and ZnO as a photocatalyst. The two tested models (Chick-Watson and Hom) fitted the kinetic of disinfection of E. coli. However, it must be noticed that, the simple agreement between experimental data and model predictions does not necessarily prove that either of the models is mechanistically correct. For the disinfection of L. Helveticus, neither of the two models fitted the experimental plots. The divergence existing between experimental and modelling results proves only that the empirical models can’t be generalized to all deactivated microorganisms.
2009 - Efficiency of nitrogen removal and protist communities the potential f...WALEBUBLÉ
Article published in the International Workshop on Integrated vision of urban and agro-industrial wastewater treatment, monitoring and reclamation: key role played by the Waste Water Treatment Plant. 2-3 Julio, 2009. ISRIM / LIFE (CEE n. 1973/92 EU Financial Instrument for the Environment) , Terni, Italy.
2010 - A new species of genus Metacystis from a Wastewater Treatment PlantWALEBUBLÉ
ABSTRACT. Unusual prostomatid specimens were found in the biological reactor of a wastewater treatment plant in a health resort in Valencia, Spain. These ciliates were attached to flocs unlike other free-swimming prostomatid ciliates described to date in the mixed liquor of activated sludge plants. The morphological study of this species led to a typically different combination of characteristics: elongated
cell shape, 20–30 somatic kineties, 2 perioral kineties, and 1 circumoral kinety, 1 large vacuole protruding at the terminal end, a lorica tapered toward the aperture with a smooth neck, and 11–16 annular ridges. These characteristics place this representative as a new species of the genus Metacystis—Metacystis galiani n. sp. This species became the dominant population within the biological reactor when high values of conductivity (4,244 mS/cm) and temperature (26.8 1C) were recorded.
Professor Cristina Nerin. Professor of Analytical Chemistry. University of Zaragoza, Spain discusses the benefits of Collisional Cross Section measurements in Ion Mobility for the confirmation of food contaminant in packaging.
this presentation is about the control and measurements of pH and red-ox potential in a fermenter or a bioreactor. there are several parameters that should be monitored in order to get the maximum productivity in a fermenter. in that few parameters are very much important to measure as well adjust to its optimum level to get the high yield.
abstract
Extracts of the medicinal plant Palicourea rigida Kunth, popularly known as douradinha, are
widely used for treating urinary tract disorders. Unfortunately, nowadays this is one of the
species endemic to Brazilian Cerrado that is at greatest risk of extinction.
The aim of the this work was to use AFLP molecular markers to determine the genetic
structure and diversity of eight natural populations of P. rigida and to associate their genetic
characteristics with loganin production in order to obtain provide relevant information
to promote programs for the conservation of this valuable medicinal plant.
A total of 120 polymorphic bands were scored and higher proportion of genetic diversity
was found in inter-populations (64%) rather than in intra-populations (36%). Fst value was
found to be significantly greater than zero (0.3601), demonstrating the complex genetic
structure of P. rigida populations. Accessions collected from Cristalina, GO, showed higher
percentage of polymorphic loci (65.5%) and the highest genetic diversity. Analysis of
Molecular variance (AMOVA) demonstrated 63.9% of intra-population genetic variation.
The lowest genetic variability was detected among accessions from the population found
in Sacramento, MG. No spatial standard was observed for P. rigida population, suggesting a
partially isolated island model. It was observed a minor but significant positive correlation
(r ¼ 0.22) between chemical and genetic matrices. The association between chemical and
genetic data indicated that environmental factors promoted the loganin production in
populations growing in Luziânia, GO, and therefore accessions from those populations
should be considered as prime material for initiating the conservation process of P. rigida.
2013 Elsevier Ltd. All rights reserved.
2017 - Environmental Ordination of Filamentous Bacteria in Activated SludgeWALEBUBLÉ
Reference:
Zornoza, A., Serrano, S. and Alonso, J.L. (2017) Environmental Ordination of Filamentous Bacteria in Activated Sludge. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
2017 - Plausible Bioindicators of Biological Nitrogen Removal Process in WWTPsWALEBUBLÉ
Reference:
Zornoza, A., Alonso, J.L. and Serrano, S. (2017) Plausible Bioindicators of Biological Nitrogen Removal Process in WWTPs. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
Modelling the Kinetic of UV Water DisinfectionMichael George
Ultraviolet disinfection is an attractive tool for treating water and eliminating pathogens with safe and available technology especially in developing countries where waterborne diseases cause the death of thousands of people every year. Even though UV is an easy tool to perform disinfection, concerns over the potential of microorganism reactivation constitute an issue for its development. In order to avoid this phenomenon, estimating the right dose of UV irradiance, the number of viable microorganisms and the sufficient contact time are important parameters to consider when performing UV disinfection. For this purpose, it’s current to use mathematical modelling. This work aimed to study the modelling of the kinetic of water disinfection by UV irradiation. Two kinetic models (Chick-Watson and Hom) were tested as to ability to scale disinfection of Gram negative Escherichia coli and Gram positive Lactobacillus Helveticus by different ultraviolet (UV) light inactivation process: UV alone, UV and TiO2 as a photocatalyst and finally UV and ZnO as a photocatalyst. The two tested models (Chick-Watson and Hom) fitted the kinetic of disinfection of E. coli. However, it must be noticed that, the simple agreement between experimental data and model predictions does not necessarily prove that either of the models is mechanistically correct. For the disinfection of L. Helveticus, neither of the two models fitted the experimental plots. The divergence existing between experimental and modelling results proves only that the empirical models can’t be generalized to all deactivated microorganisms.
2009 - Efficiency of nitrogen removal and protist communities the potential f...WALEBUBLÉ
Article published in the International Workshop on Integrated vision of urban and agro-industrial wastewater treatment, monitoring and reclamation: key role played by the Waste Water Treatment Plant. 2-3 Julio, 2009. ISRIM / LIFE (CEE n. 1973/92 EU Financial Instrument for the Environment) , Terni, Italy.
2010 - A new species of genus Metacystis from a Wastewater Treatment PlantWALEBUBLÉ
ABSTRACT. Unusual prostomatid specimens were found in the biological reactor of a wastewater treatment plant in a health resort in Valencia, Spain. These ciliates were attached to flocs unlike other free-swimming prostomatid ciliates described to date in the mixed liquor of activated sludge plants. The morphological study of this species led to a typically different combination of characteristics: elongated
cell shape, 20–30 somatic kineties, 2 perioral kineties, and 1 circumoral kinety, 1 large vacuole protruding at the terminal end, a lorica tapered toward the aperture with a smooth neck, and 11–16 annular ridges. These characteristics place this representative as a new species of the genus Metacystis—Metacystis galiani n. sp. This species became the dominant population within the biological reactor when high values of conductivity (4,244 mS/cm) and temperature (26.8 1C) were recorded.
Professor Cristina Nerin. Professor of Analytical Chemistry. University of Zaragoza, Spain discusses the benefits of Collisional Cross Section measurements in Ion Mobility for the confirmation of food contaminant in packaging.
this presentation is about the control and measurements of pH and red-ox potential in a fermenter or a bioreactor. there are several parameters that should be monitored in order to get the maximum productivity in a fermenter. in that few parameters are very much important to measure as well adjust to its optimum level to get the high yield.
abstract
Extracts of the medicinal plant Palicourea rigida Kunth, popularly known as douradinha, are
widely used for treating urinary tract disorders. Unfortunately, nowadays this is one of the
species endemic to Brazilian Cerrado that is at greatest risk of extinction.
The aim of the this work was to use AFLP molecular markers to determine the genetic
structure and diversity of eight natural populations of P. rigida and to associate their genetic
characteristics with loganin production in order to obtain provide relevant information
to promote programs for the conservation of this valuable medicinal plant.
A total of 120 polymorphic bands were scored and higher proportion of genetic diversity
was found in inter-populations (64%) rather than in intra-populations (36%). Fst value was
found to be significantly greater than zero (0.3601), demonstrating the complex genetic
structure of P. rigida populations. Accessions collected from Cristalina, GO, showed higher
percentage of polymorphic loci (65.5%) and the highest genetic diversity. Analysis of
Molecular variance (AMOVA) demonstrated 63.9% of intra-population genetic variation.
The lowest genetic variability was detected among accessions from the population found
in Sacramento, MG. No spatial standard was observed for P. rigida population, suggesting a
partially isolated island model. It was observed a minor but significant positive correlation
(r ¼ 0.22) between chemical and genetic matrices. The association between chemical and
genetic data indicated that environmental factors promoted the loganin production in
populations growing in Luziânia, GO, and therefore accessions from those populations
should be considered as prime material for initiating the conservation process of P. rigida.
2013 Elsevier Ltd. All rights reserved.
Use of stable and radio isotopes to understand the plant physiological processRAHUL GOPALE
Introduction
what is isotope ?
Types of Isotopes
Isotopic Labelling
ADVANTAGES AND DISADVANTAGES OF ISOTOPIC STUDY
APPLICATIONS OF ISOTOPES IN AGRICULTURE
Principle isotopes used in plant-soil studies
Case studies
FUTURE THRUSTS OF ISOTOPIC STUDY
CONCLUSIONS
REFERENCES
Rapid identification of dermatophyte species by 28S rDNA Polymerase Chain Rea...iosrjce
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
Pixe for Elemental Analysis of Domestic Medicinal Plants in Bangladeshijrap
The medicinal plants are a source of biologically important elements which are necessary both for animals
and plants. Present study investigates the concentration of elemental constituents of seven selected
medicinally important plants of Bangladesh, namely Terminalia bellirica, Centella asiatica, Pleurotus
ostreatus, Curcuma longa, Bombax ceiba and Trigonella foenum-graecum. Appropriately desiccated
samples (pellets) of these medicinal plants were bombarded with accelerated proton beam with the help of
3 MV Van de Graff accelerator at accelerator Facilities Division, Atomic Energy Center, Dhaka. Eleven
different elements- P, S, K, Ca, Sc, Ti, V, Mn, Fe, Cd and I were detected by collecting the harvested X-rays
with [Si(Li)] detector. Data collection and analysis were performed using Maestro-32 and GUPIX
software
Polymeric Nanoparticles of Rifampicin were prepared by emulsion solvent evaporation technique using poly methyl methacrylate as polymer matrix and Poly vinyl alcohol as surfactant. Drug entrapped free flowing nanoparticles of Rifampicin were obtained after optimization using 32 factorial design and characterized for entrapment efficiency, particle size distribution, differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM) and in vitro and stability studies. The PMMA nanoparticles had a small size (213 ± 0.72 nm), uniform size distribution. The effects of dependent variables drug-polymer ratio and surfactant concentration on particle size and encapsulation efficiency were studied. The drug and polymer were not interacting with each other. SEM studies revealed the spherical shape of nanoparticles and in vitro release studies showed sustained drug release. RIF-polymeric nanoparticles drug delivery system proved to be promising for anti-tubercular therapy.