Chromovert® technology can generate stable cell lines that express multiple genes encoding an ion channel protein. The document describes using Chromovert® to isolate several clones that stably expressed three subunits of a heterotrimeric ion channel. Optimal expression and functional activity of the ion channel required specific mRNA expression levels and maintaining the cell lines in specialized media, as the ion channel was toxic otherwise. The resulting cell line was validated in high throughput screening, consistently achieving high Z-prime scores, demonstrating its robustness for drug screening.

1. Chromovert® Produces Clones Stably and Functionally
Expressing a Multigene Ion Channel
Jessica Langer, Alane Taratuska, Purvi Shah, Lori Schmid and Kambiz Shekdar
Chromocell Corporation, 675 U.S. Highway One, North Brunswick, NJ
Abstract
Chromovert® is a technology for detecting multiple
mRNAs in living cells using sequence-specific
fluorogenic probes to report the presence of target
RNAs. When hybridized to target sequences, probes
undergo fluorogenic conformational changes, separating
fluorophores from the vicinity of quenchers resulting in
detectable fluorescent signals at defined wavelengths.
Multiple probes incorporating different fluorophores,
each directed against a different target RNA, are
simultaneously resolved using flow cytometry.
Using Chromovert® we isolated multiple clones triple
positive for a heterotrimeric ion channel. As expected the
functional ion channel is toxic unless cells are
maintained in optimized media. Interestingly, specific
mRNA stoichiometry is associated with optimal
functional activity.
Using the FDSS6000 platform the stable recombinant cell
line consistently generates Z’ of 0.8.
Conclusion: Chromovert® produces a stable triple
recombinant cell line suitable for quality high throughput
screening.
Introduction
Chromocell® has commercialized a proprietary
technology, Chromovert®, enabling the rapid production
of high quality stable cell lines facilitating robust cell-
based assays and improved antibody production. A key
feature of Chromovert® is the ability to analyze individual
living cells without compromising cell viability. Millions
of cells are analyzed and multigene cells, expressing
target RNA (triple positive), are isolated by cell sorting.
Here we use Chromovert® to build and then validate in a
robust HTS assay a stable, functional constitutive cell
line for a previously inaccessible three-subunit ion
channel target.
Stable triple recombinant cell line loaded using MDCC No
Wash MP Dye and assayed using Hamamatsu FDSS6000.
Agonist addition traces in red, buffer addition traces in blue.
Z’ = 0.80. (Data analysis using CeuticalSoft).
Chromovert® :Application
(A) The fluorogenic probes are short oligonucleotides comprised of
a central stretch of nucleotides complementary to the target RNA
sequence and mutually complementary termini. One terminus is
covalently bound to a fluorophore and the other to a quenching
moiety. In the absence of target, the termini are hybridized such
that the fluorophore and the quencher are in close proximity and
little or no fluorescence is produced. (B) When hybridized to its
target sequence, the probe undergoes a spontaneous fluorogenic
conformational change that displaces the fluorophore from the
vicinity of the quencher, resulting in a detectable fluorescent signal.
Chromovert®
:Theory
Target RNA
A B
Chromovert®
:Procedure
+
(A) Cells are transfected with expression plasmids containing
genes of interest. (B) Cells are transfected with fluorogenic
probes and individual cells which fluoresce above background
are isolated by flow cytometric cell sorting.
A
B
Chromovert®
:Validation
Genomic PCR of clones for
integrated sequences
Conclusions
1.Chromovert® technology
generates multigene clones.
2.Optimal functional activity is
associated with mRNA
stoichiometry and appropriate
cell background.
3.Ion channel was toxic to cells
unless grown in special media.
4.Clones are stable for months.
5.Functional assays are robust
with Z’=0.80.
Chromovert® generates
multiple clones with a high
degree of accuracy. Cells
sorted for triple positive
gene expression were
confirmed using genomic
PCR with 85% confirmation,
9% double positive, 6%
single positive.
1000
10000
100000
1000000
10000000
a b g
Fold
mRNA
increase
Functional channel activity is
associated with
stoichiometric mRNA
expression of the three
genes. Results emphasize
importance of generating
and testing multiple clones
for optimal functional activity.
Acknowledgement: Chromocell® expresses
its appreciation to Hamamatsu Photonic
Systems for their technical and application
support for the FDSS6000.
Stable
Cell Line
Screen Clones
for Function
Transfect
Probes& Sort
Transfect
Target
Chromovert® :Summary
Chromovert ® cell line production is efficient since
millions of cells are sorted, cell viability is preserved, and
numerous clones are isolated for study. This ion channel
was toxic, requiring special cell media and cell line
background choice was critical for function. Time for cell
line generation is 1-3 months on average.