This document outlines a workshop for compiling literature reviews in the life sciences. It discusses the purpose of literature reviews and identifying relevant sources. The workshop covers searching databases, following citations, documenting research, and staying current. Attendees are introduced to literature review goals and examples, key databases, citation tracking tools, citation managers, and asking subject librarians for assistance. The goal is to equip attendees with strategies for conducting a thorough literature review.
Entering words into a search engine is great for
finding a quick answer but it won’t always give
you the best evidence for your arguments. In this
session learn how to research rather than search for
the best information for your assignments.
Entering words into a search engine is great for
finding a quick answer but it won’t always give
you the best evidence for your arguments. In this
session learn how to research rather than search for
the best information for your assignments.
An introduction to APA literature reviews for middle schoolers.
Here's the set:
http://www.slideshare.net/samlandfried/9th-grade-may-11-lit-review-activity
http://www.slideshare.net/samlandfried/9th-grade-may-11-lesson-plan
http://www.slideshare.net/samlandfried/9th-grade-april-20-literature-review
http://www.slideshare.net/samlandfried/9th-grade-april-20-lit-review-handout
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For Training/Workshop/Seminars/Conference/Collaboration mail to – manuscriptpedia@gmail.com and director@manuscriptpedia.com (cc to)
An introduction to APA literature reviews for middle schoolers.
Here's the set:
http://www.slideshare.net/samlandfried/9th-grade-may-11-lit-review-activity
http://www.slideshare.net/samlandfried/9th-grade-may-11-lesson-plan
http://www.slideshare.net/samlandfried/9th-grade-april-20-literature-review
http://www.slideshare.net/samlandfried/9th-grade-april-20-lit-review-handout
Follow our LinkedIn Page for future events - linkedin.com/company/manuscriptpedia
Follow our Facebook Page for Future updates - https://fb.me/manuscriptpediaindia
Follow our Facebook Group for Future updates - www.facebook.com/groups/manuscriptpedia
Join our Telegram Group - https://t.me/Manuscriptpedia
For free research support call - +91 9150929629 / mail to manucriptpedia@gmail.com
For Training/Workshop/Seminars/Conference/Collaboration mail to – manuscriptpedia@gmail.com and director@manuscriptpedia.com (cc to)
Literature Review (Review of Related Literature - Research Methodology)Dilip Barad
Literature Review or Review of Related Literature is one of the most vital stages in any research. This presentation attempts to throw some light on the process and important aspects of literature review.
Literature review for a dissertation: a step-by-step guideOlga Koz, DM, MLS
A guide for doctoral students to the process of conducting literature searching, analysis, organizing, synthesis and writing a literature review for a dissertation
This presentation accompanies a Malayalam video on writing literature reviews in Social Sciences.
The video can be found at https://www.youtube.com/c/DrChinchuC
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
2. Agenda
1. Introductions
2. About Literature Reviews
3. Literature Sources
4. Tools & Strategies
5. For More Information
3. Introductions
• Name?
• Department?
• Why are you here today?
• Previous library workshops?
4. Companion Workshops
• Writing Literature Reviews (Writing Center)
• “Everything You Need to Know” (Libraries)
• “Keeping Up With the Literature” (Libraries)
• “Publishing Your Research” (Libraries)
• Workshops on specific databases (Libraries)
• Workshops on citation managers (Libraries)
5. Definitions
• “A literature review is a written document that
presents a logically argued case founded on a
comprehensive understanding of the current state of
knowledge about a topic of study. This case
establishes a convincing thesis to answer the study’s
question.”
- Literature review: Six steps to success (Machi,McEvoy)
• Introduces theories
• Shares results of related studies & identifies gaps
• Locates your research & results within dialogue
- Research design: Qualitative, quantitative, and mixed method
approaches (Creswell)
6. Know your Goal
• What is expected of me?
• Does my research make a unique contribution?
• Is it feasible?
• Do I understand the context?
• What are the key methodologies, issues, or
controversies?
• Do I need to modify my topic?
• Time management:
– Dissertation & assignment calculators
7. Seek out Examples
• Primary research articles
• Review articles, meta-analyses
• Theses & dissertations
– Databases
• Ask yourself:
– How long?
– How many sources?
– Placement?
8. Identify Sources
• Books
– Start with: Encyclopedias, dictionaries, handbooks
• Journal articles
– Start with: Review articles, meta-analyses
• Conference proceedings
– Start with: Big international conferences
• Other
– Gov’t documents, technical reports, white papers…
• How to find = Databases (see handout)
9. Searching Databases
• AND, OR, NOT
• “Phrases in quotes”
• Truncation, wildcard symbols * ?
• Limits:
– Article Title
– Journal / Source / Publication Title
– Subject / Descriptor / Classification
– Many more...
• Obtaining full text of articles: “Find It”
10. Follow Citations
• Backwards + forwards
• Web of Knowledge / Web of Science
– “References”
– “Times Cited”
– “Cited Reference Search”
• Journal Citation Reports (JCR)
– “Impact Factor”
11. Document your Research
• Read, take notes, & map sources
– Concept map tools (see Wikipedia)
• Compile & map search terms:
– Synonyms, broader, narrower, and related terms
– Thesauri, taxonomies, ontologies (ex: MeSH, LCSH)
– Scientific terms (ex: Latin names, chemical names)
– Historical terms
– International terms / alternate spellings
• Manage citations, documents & notes
– http://library.wisc.edu/citation-managers
12. Stay Current
• Email alerts & RSS feeds:
– Journal issue tables of contents
– Keywords
– Citations
13. Ask a Librarian
• Your Liaison Librarian
- http://library.wisc.edu/directory/liaisons
• Drop in (desk or office)
• Chat
• Phone
• E-mail
• Appointments
14. Good Luck!
• Thank you for coming
• Evaluation (Grad Student Collaborative)
– http://grad.wisc.edu/gsc/evals
Editor's Notes
Amanda Werhane
librarian at Steenbock Library
Steenbock primarily serves CALS, SOHE, Vet Med, Nelson IES, UW Extension
open to everyone, popular with undergrads in nearby dorms
liaison librarian for the plant & insect science departments in the College of Agricultural & Life Sciences
Masters degree in librarianship 5 years ago
Bachelors degree in Conservation Biology 14 years ago
* How many of you have met your department’s liaison librarian?
Writing Center website
Library Workshops by date and Workshops by subject website
Logically argued case – not just an annotated list of articles
Comprehensive, state of knowledge – broad and deep
Establishes a convincing thesis – clearly provides a context and establishes the basis for your research
Primary research articles
Every scholarly article cites previously-published research
http://library.wisc.edu – Databases – Top 10 – Web of Science – All Databases – madison in address – current week
Pick an article to look at – Find It
“Examination of Xenorhabdus nematophila lipases in pathogenic and mutualistic host interactions reveals a role for xlpA in nematode progeny production”
Review articles:
“Vertebrate maternal-effect genes: Insights into fertilization, early cleavage divisions, and germ cell determinant localization from studies in the zebrafish”
Theses & dissertations:
http://library.wisc.edu – Databases – dissertations – Search – Theses & Dissertations – Advanced – Goldman Irwin in Advisor
“Development of Carrot…” – Chapter 1 is “Review of Relevant Literature” – browse to Ch.1 references
Types of literature: books, journal articles, government documents, websites, dissertations,
Publications back to 1922, and something “in press”