Column chromatography is a technique used to separate mixtures based on how strongly components interact with a stationary phase. The mixture is dissolved in a solvent and passed through a column packed with an adsorbent material. Components that more strongly interact with the adsorbent move through the column more slowly and are separated from those that interact less strongly. After development, the column can be eluted to recover the separated components. Key factors that affect column efficiency include the choice of solvent, dimensions of the column, particle size of the adsorbent, pore size, and temperature. Column chromatography has applications in analytical separations and purification of isomers, diastereomers, tautomers, and racemates.
It includes principle, mobile phase , stationary phase , mechanism of separation , types of paper chromatography, operating procedure, identification , detection , applications and limitations of paper chromatography
Mixtures of compounds are very common in Organic Chemistry. Most reactions produce more than one product. Naturally occurring materials are only rarely 100% pure. It is therefore desirable to have a simple, fast and efficient way to determine the purity of Organic mixtures. The separation of a mixture by passing it, in solution, over an adsorbent (such as Alumina or Silica Gel) is the basic idea of Chromatography. Chromatography is a very general phenomenon. It involves the passage of a mobile phase across a stationary phase in a column. Usually a mixture of compounds is present in the mobile phase
It includes principle, mobile phase , stationary phase , mechanism of separation , types of paper chromatography, operating procedure, identification , detection , applications and limitations of paper chromatography
Mixtures of compounds are very common in Organic Chemistry. Most reactions produce more than one product. Naturally occurring materials are only rarely 100% pure. It is therefore desirable to have a simple, fast and efficient way to determine the purity of Organic mixtures. The separation of a mixture by passing it, in solution, over an adsorbent (such as Alumina or Silica Gel) is the basic idea of Chromatography. Chromatography is a very general phenomenon. It involves the passage of a mobile phase across a stationary phase in a column. Usually a mixture of compounds is present in the mobile phase
Chromatography is a powerful and advanced techniques for separating mixtures. Many types of chromatographic techniques are known, such as paper, thin layer, column chromatography, each with its own strength and weakness.
Chromatography system in general have a stationary phase and a mobile phase.
In column chromatography both phases are placed in a column container, i.e. all the chromatographic operations are carried out using column.
Column chromatography in chemistry is a method using for the Identification, separation and purify individual chemical compounds from mixtures of compounds in the large amount.
Column Chromatography is a separation technique in which components of mixture is separated by using a glass column packed with stationary phase and liquid mobile phase flowing continuously through the column.
It is suitable for the physical separation of gram quantities of material. A solvent acts as the mobile phase while a finely divided solid surface acts as the stationary phase.
Usually a glass tube with a diameter from 1cm to 10cm and a height of 20 cm to 50cm with a tap at the bottom, is used for this purpose.
Depending upon the flow of solvent down, column chromatography may be separated into two categories.
Gravity column chromatography
If the solvent is allowed to flow down the column by Gravity, or downward process, it is known as gravity column chromatography .
Flash chromatography
If the solvent is forced down the column by positive air pressure , it is called flash chromatography.
The present slide gives us an insight into the different aspects of application of columnn chromatrography the principle behind it and at the same time its recent advances.
This presentation contains all the topics related to column chromatography. That includes introduction, principle,apparatus, experimental aspects of column chromatography, application of column chromatography, advantage and disadvantage of column chromatography with reference.
It is also known as vaccume chromatography.
It is one of the type of oldest technique.
It is modified from of flash chromatography.
It is column chromatography technique, principle is based on partition chromatography.
It is also known as medium phase chromatography.
It can consist of stationary phase and mobile phase. It is important for separation, identification, purification of compound.
It is a glass liquid phase chromatographic technique.
It is modified from of flash chromatography.
It is used for the separation of plant pigments and metabolites.
It is used as qualitative analysis and quantitative analysis.
Gout is a common and complex form of arthritis that can affect anyone. It's characterized by sudden, severe attacks of pain, swelling, redness and tenderness in one or more joints, most often in the big toe
Typhoid fever is a life-threatening infection caused by the bacterium Salmonella Typhi. It is usually spread through contaminated food or water. Once Salmonella Typhi bacteria are ingested, they multiply and spread into the bloodstream
Tuberculosis (TB) is an infectious disease that most often affects the lungs and is caused by a type of bacteria. It spreads through the air when infected people cough, sneeze or spit. Tuberculosis is preventable and curable. About a quarter of the global population is estimated to have been infected with TB bacteria
It includes Defination,Classification of powders.Special Types of Powders like effervesent,effloroscent,Eutectic mixture.Fomulation of powder with mixing technique of powder.
Two-dimensional nuclear magnetic resonance spectroscopy (2D NMR) is a set of nuclear magnetic resonance spectroscopy (NMR) methods which give data plotted in a space defined by two frequency axes rather than one.
Types of 2D NMR include correlation spectroscopy (COSY), J-spectroscopy, exchange spectroscopy (EXSY), and nuclearOverhauser effect spectroscopy (NOESY).
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
HOT NEW PRODUCT! BIG SALES FAST SHIPPING NOW FROM CHINA!! EU KU DB BK substit...GL Anaacs
Contact us if you are interested:
Email / Skype : kefaya1771@gmail.com
Threema: PXHY5PDH
New BATCH Ku !!! MUCH IN DEMAND FAST SALE EVERY BATCH HAPPY GOOD EFFECT BIG BATCH !
Contact me on Threema or skype to start big business!!
Hot-sale products:
NEW HOT EUTYLONE WHITE CRYSTAL!!
5cl-adba precursor (semi finished )
5cl-adba raw materials
ADBB precursor (semi finished )
ADBB raw materials
APVP powder
5fadb/4f-adb
Jwh018 / Jwh210
Eutylone crystal
Protonitazene (hydrochloride) CAS: 119276-01-6
Flubrotizolam CAS: 57801-95-3
Metonitazene CAS: 14680-51-4
Payment terms: Western Union,MoneyGram,Bitcoin or USDT.
Deliver Time: Usually 7-15days
Shipping method: FedEx, TNT, DHL,UPS etc.Our deliveries are 100% safe, fast, reliable and discreet.
Samples will be sent for your evaluation!If you are interested in, please contact me, let's talk details.
We specializes in exporting high quality Research chemical, medical intermediate, Pharmaceutical chemicals and so on. Products are exported to USA, Canada, France, Korea, Japan,Russia, Southeast Asia and other countries.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
2. COLUMN CHROMATOGRAPHY
Principle :
The mixture to be separated is dissolved in a suitable solvent and allowed to pass through
a to be component which has greater adsorbing power is adsorbed in the upper part of the
column. The next component is adsorbed in the column, which has lesser adsorbing power
than the first component. This process is continued. As a result the materials are partially
separated & adsorbed in the various parts of the column. The initial separation of the
various components can be improved by passing either the original or some other suitable
solvent slowly through the column.
The various bonds in the column become more defined. The banded column of adsorbent
is termed a chromatogram & the operation is spoken of as development of chromatogram.
The portion of a column, which is occupied by a particular substance, is called its zone.
The zones contain the substance which can be separated by two propellers:
A. After development, the column of adsorbent may be posted out of to be, the various
zones are cut with a knife at boundaries and the substances present in zones
extracted with a suitable solvent. The process of recovery of constituents form the
chromatogram is known as elution.
B. After development, the column may be washed with more solvent, now termed as
event and each component is collected separately as it reacts and of the column and
is released.
APPARATUS:
A Simple glass tube with a stopper cork at one end is taken; having 20-30 cm length and
2-3cm diameter long enough to carry 50-100 gm of adsorbent and may retain several grams
of adsorbent. The adsorbent is supported by plug of cotton or glass wool. Long and narrow
tubes are used for difficult separation.
3. Fig.8- Apparatus for column chromatography.
ADSORBENTS REQUIREMENT:
Particles should be spherical in shape and uniform in size.
Their mechanical stability must be great enough to prevent the formation of fine
dust which might deposited in the channels of the packing.
They should not react chemically either with the eluting solvent or with the
sample components.
They should contain as small amount of soluble components as possible.
They should be catalytically inactive and have neutral surface.
Suppose talc, starch, insulin, sodium carbonate, calcium carbonate, calcium phosphate,
magnesium carbonate, lime, activated silicic acid, activated cilicic acid, activated
magnesium silicate, activated alumna and fullers earth are the most common adsorbents.
Preparationof Adsorption column:
The glass wool or cotton plug is used as a support for the column. It is first kept in position
in the tube. The tube is then clamped vertically. Now the adsorbent is added in portion so
that the tube is packed uniformly with the adsorbent. Whenever and portion is added it is
pressed from above with a flattened glass rod before next portion is added. This is
continued till nearly two third of it is filled. Now either eluting solvent or
4. petroleum ether is passes and bottom and is connected with section. It is important that the
surface of column is ether covered with eluting solvent or the pet either.
=> SOLVENTS:
The choice of solvent will naturally depend in the frits place upon the solubility relations of
substance. The solvents generally employed pusses boiling points between 400 and 850
C. the must widely used is light petroleum and others are cyclohoxane, carbon disulphide,
benzene, chloroform, carbon tetra chloride, methylene chloride, ethyl acetate, ethyl alcohol,
acetone, ether and acetic acid.
The solvents used in chromatography have three functions.
1) They serve to introduce the mixture of the column.
2) They affect the process of development by which the zone of chromatogram is
separated to their full extent.
3) They are also used to remove the required content of each zone from mechanically
separated parts of the column or from the column as a whole after it is properly
developed.
FACTORS AFFECTING COLUMN EFFICIENCY:
A. Nature of solvents:
Solvents of law viscosities are generally used for high efficiency separations. The
reason for this is that rate of flow is inversely proportional to viscosity and hence
it becomes necessary to select a solvent of lower viscosity and proper elution
strength.
B. Dimensions of column:
It is possible to improve the column efficiency by increasing the length/width ratio
of the column. For common preparative separation sample/column packing ratios
have found to range from 1:20 to 1:100.
C. Particle size column packing:
It is possible to increase the column efficiency by decreasing the particle size of
the adsorbent. The usual particle size ranges from 100 to 200 meshes.
D. Pore diameters of column packing:
Polar adsorbent posses a pore diameter of ≤ 20 A0, A decrease in average pore
5. diameter from 170 – 20 A0 does not affect efficiency.
E. Temperature of column:
Difficult soluble samples are generally at higher temperatures while other samples
are separated at room temperature.
APPLICATIONS:
For analytical uses.
Separation of geometric isomers. I.e. cisltrans.
Separation of diameters and Tautumeric mixtures.
Separation of racemates.