This document discusses colchicine-induced polyploidy and mutational breeding in trees. It provides information on colchicine and how it induces polyploidy by inhibiting chromosome segregation. It also describes methods for inducing triploidy in Eucalyptus urophylla through colchicine treatment of megaspores at different developmental stages. The results show that 0.25% colchicine treatment produced some triploid plants. Mutational breeding techniques aim to induce genetic mutations to generate genetic variation for plant breeding. Various physical and chemical mutagens are discussed along with their mode of action and the types of mutations they cause. The document also outlines the key steps involved in mutational breeding programs