Chitosan (2-amino-2deoxy-(1→4)-β-D-glucopyranan), a polyaminosaccharide, normally obtained by alkaline deacetylation of chitin is the principal component of living organisms such as fungi and crustacea.
Chitosan (2-amino-2deoxy-(1→4)-β-D-glucopyranan), a polyaminosaccharide, normally obtained by alkaline deacetylation of chitin is the principal component of living organisms such as fungi and crustacea.
Chitosan as Promising Materials for Biomedical Application: Review -Crimson P...CrimsonPublishersRDMS
Chitosan as Promising Materials for Biomedical Application: Review by Raghvendrakumar M* in Crimson Publishers: Peer Reviewed Material Science Journals
chitosan nanoparticles synthesis and application in various fields i.e. biocompatible fruit preservatives, water treatment with non toxic substrate, cotton functionalization, etc.
Microcrystalline Cellulose (MCC) Manufacturing Industry. Production of Pharmaceutical Grade Microcrystalline Cellulose
Microcrystalline Cellulose Market Projected to Reach 1540 Million US$ by 2025, at a CAGR of 6.3%
Microcrystalline cellulose (C6H10O5)n is refined wood pulp. It is a white, free-flowing powder. Chemically, it is an inert substance, is not degraded during digestion and has no appreciable absorption. In large quantities it provides dietary bulk and may lead to a laxative effect.
Microcrystalline Cellulose (MCC) is a partially depolymerized specialty cellulose prepared by treating α-cellulose. MCC is widely used in pharmaceutical, food & beverage, cosmetic and other industrial applications, owing to its broad spectrum of properties. MCC is used a suspension stabilizer and an excipient, owing to its chemical inertness and non-toxic nature.
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Niir Project Consultancy Services
An ISO 9001:2015 Company
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
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#Production_of_Microcrystalline_Cellulose, #Microcrystalline_Cellulose_(MCC), #Manufacturing_Process_for_Microcrystalline_Cellulose_(MCC), How is Microcrystalline Cellulose made? Microcrystalline Cellulose Plant, Process for Producing Microcrystalline Cellulose, #Microcrystalline_Cellulose_(MCC)_Production, Microcrystalline Cellulose Manufacturing Process, Preparation and Characterization of Microcrystalline Cellulose (MCC), #Processing_of_Pharmaceutical_Grade_Microcrystalline_Cellulose, Preparation of Microcrystalline Cellulose, Microcrystalline Cellulose, Manufacture of Microcrystalline Cellulose, Microcrystalline Cellulose Manufacturing Plant, Microcrystalline Cellulose Production, #Project_Report_on_Microcrystalline_Cellulose_Manufacturing_Industry, Detailed Project Report on Microcrystalline Cellulose (MCC) Production, Project Report on Microcrystalline Cellulose (MCC) Production, #Pre_Investment_Feasibility_Study_on_Microcrystalline_Cellulose_(MCC)_Production, Techno-Economic feasibility study on Microcrystalline Cellulose (MCC) Production, #Feasibility_report_on_Microcrystalline_Cellulose_(MCC)_Production, #Free_Project_Profile_on_Microcrystalline_Cellulose_(MCC)_Production, Project profile on Microcrystalline Cellulose (MCC) Production, Download free project profile on Microcrystalline Cellulose (MCC) Production, #Microcrystalline_Cellulose_(MCC)_Industry, How to Make Microcrystalline Cellulose
In the recent years, bio-based and biodegradable products have raised great interest since sustainable development policies tend to expand with the decreasing reserve of fossil fuel and the growing concern for the environment. Bio-Polymers are a form of polymers derived from plant sources such as sweet potatoes, soya bean oil, sugarcane, hemp oil, and corn starch. These polymers are naturally degraded by the action of microorganisms such as bacteria, fungi and algae. Bio-plastics can help alleviate the energy crisis as well as reduce the dependence on fossil fuels of our society. They have some remarkable properties which make it suitable for different applications. This paper tries to give an insight about Bio-plastics, their composition, preparation, properties, special cases, advantages disadvantages, commercial viability, its life cycle, marketing and pricing of these products.
As a result, the market of these environmentally friendly materials is in rapid expansion,
10 –20 % per year.
Natural polymers by Dr. khlaed shmareekhخالد شماريخ
the presentation is about the natural polymers i.e. classification, applications, properties and examples. it is in 25 pages in shortcuted manner and simple method.
It is a Complexaing agent.
Synonym: cavitron, cycloamyloses, cycloglucan, cyclic oligosaccharide
It is a important for increasing the solubility of poorly water soluble drugs.
Cyclodextrines are produced from starch by means of enzymatic conversion.
They are used in food, pharmaceutical, drug delivery, and chemical industries, as well as agriculture and environmental engineering.
Cyclodextrines are composed of 5 or more α-D glucopyranoside units linked 1->4, as in amylose linkage.
Cyclodextrines contains 32 1,4-anhydroglucopyranoside units, while as a poorly characterized mixture, at least 150-membered cyclic oligosaccharides are also known. Typical cyclodextrins contain a number of glucose monomers ranging from six to eight units in a ring.
CDs, with lipophilic inner cavities & hydrophilic outer surfaces, are interacting with a guest molecule to form non covalent inclusion complexes.
Today CDs are only synthesized either by fermentation or enzymatically.
Many CGTases from different microorganisms are known, cloned, sequenced, characterized and used for production of CDs.
Brief intro about crystalline and amorphous structures,
glass transition temperature,
free volume theory of glass transition temperature,
factors effecting glass transition temperature etc.
Hydrogels are three-dimensional network of hydrophilic cross-linked polymer that do not dissolve but can swell in water or can respond to the fluctuations of the environmental stimuli
Hydrogels are highly absorbent (they can contain over 90% water) natural or synthetic polymeric networks
Hydrogels also possess a degree of flexibility very similar to natural tissue, due to their significant water content
Plastics has been evolving now a days. Our lives has been filled with plastics. Almost all of our things are made of plastics but do you what it is and what it is made of?
One of the most common and widely used plastic is polyethylene or PE with the resin codes 2 and 4. It is mostly used as plastic bags, food wraps, bulletproof vest, pipes and so many more. Here is a little preview of polyethylene and what is its purpose in our daily lives.
What is polyethylene?
Its properties, structure and applications.
polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units.
Cellulose is an important structural component of the primary cell wall of green plants, many forms of algae and the oomycetes. Some species of bacteria secrete it to form biofilms.
Cellulose is the most abundant organic polymer on Earth. The cellulose content of cotton fiber is 90%, that of wood is 40–50% and that of dried hemp is approximately 45%.
Cellulose is mainly used to produce paperboard and paper.
Smaller quantities are converted into a wide variety of derivative products such as cellophane and rayon.
Conversion of cellulose from energy crops into biofuels such as cellulosic ethanol is under investigation as an alternative fuel source.
Cellulose for industrial use is mainly obtained from wood pulp and cotton.
Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms that live in their guts, such as Trichonympha.
In humans, cellulose acts as a hydrophilic bulking agent for feces and is often referred to as a "dietary fiber".
speciality polymers are the class of some high perfomance polymers ,including plastic,polymers,fluids,membranes,smart hydrogels,and elastomers that are designed to meet the critical requirement that engineers face everyday in key industries including plastic, automobiles, aeronautics, smart devices, health care, pharmacy, energy production and storage. this article is a short but diversified study of post grad. students in the area of speciality polymers, the types and applications in engineering, technology ,life sciences and research. it include diverce applications of iconic polymer metal composites liquid crystal polymers ,synthetic polymer membranes, smart hydrogels, and dendritic polymers.
Background- Chitosan is the most abundant natural amino polysaccharide. Researchers have found that chitosan is biocompatible, biodegradable and nontoxic, which have made wide applicability in the pharmaceutical field. Objectives- Aim of the study was to prepare Chitosan from chitin and characterize them. Methods- Chitosan was prepared by deacetylation of chitin and characterized by U.V Spectrophotometry, FTIR (Fourier transform infrared spectroscopy), DLS (Dynamic Light Scattering), and Scanning electron microscopy (SEM). Results- The present study showed that Chitosan was successfully prepared by deacetylation of chitin. The obtained chitosan was characterized for further study. Conclusion- Our study confirms the preparation by Chitosan from Chitin for further study. Key-words- Chitin, Chitosan, Deacetylation, DLS, FTIR, SEM
Chitosan as Promising Materials for Biomedical Application: Review -Crimson P...CrimsonPublishersRDMS
Chitosan as Promising Materials for Biomedical Application: Review by Raghvendrakumar M* in Crimson Publishers: Peer Reviewed Material Science Journals
chitosan nanoparticles synthesis and application in various fields i.e. biocompatible fruit preservatives, water treatment with non toxic substrate, cotton functionalization, etc.
Microcrystalline Cellulose (MCC) Manufacturing Industry. Production of Pharmaceutical Grade Microcrystalline Cellulose
Microcrystalline Cellulose Market Projected to Reach 1540 Million US$ by 2025, at a CAGR of 6.3%
Microcrystalline cellulose (C6H10O5)n is refined wood pulp. It is a white, free-flowing powder. Chemically, it is an inert substance, is not degraded during digestion and has no appreciable absorption. In large quantities it provides dietary bulk and may lead to a laxative effect.
Microcrystalline Cellulose (MCC) is a partially depolymerized specialty cellulose prepared by treating α-cellulose. MCC is widely used in pharmaceutical, food & beverage, cosmetic and other industrial applications, owing to its broad spectrum of properties. MCC is used a suspension stabilizer and an excipient, owing to its chemical inertness and non-toxic nature.
See more
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Contact us:
Niir Project Consultancy Services
An ISO 9001:2015 Company
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
#Production_of_Microcrystalline_Cellulose, #Microcrystalline_Cellulose_(MCC), #Manufacturing_Process_for_Microcrystalline_Cellulose_(MCC), How is Microcrystalline Cellulose made? Microcrystalline Cellulose Plant, Process for Producing Microcrystalline Cellulose, #Microcrystalline_Cellulose_(MCC)_Production, Microcrystalline Cellulose Manufacturing Process, Preparation and Characterization of Microcrystalline Cellulose (MCC), #Processing_of_Pharmaceutical_Grade_Microcrystalline_Cellulose, Preparation of Microcrystalline Cellulose, Microcrystalline Cellulose, Manufacture of Microcrystalline Cellulose, Microcrystalline Cellulose Manufacturing Plant, Microcrystalline Cellulose Production, #Project_Report_on_Microcrystalline_Cellulose_Manufacturing_Industry, Detailed Project Report on Microcrystalline Cellulose (MCC) Production, Project Report on Microcrystalline Cellulose (MCC) Production, #Pre_Investment_Feasibility_Study_on_Microcrystalline_Cellulose_(MCC)_Production, Techno-Economic feasibility study on Microcrystalline Cellulose (MCC) Production, #Feasibility_report_on_Microcrystalline_Cellulose_(MCC)_Production, #Free_Project_Profile_on_Microcrystalline_Cellulose_(MCC)_Production, Project profile on Microcrystalline Cellulose (MCC) Production, Download free project profile on Microcrystalline Cellulose (MCC) Production, #Microcrystalline_Cellulose_(MCC)_Industry, How to Make Microcrystalline Cellulose
In the recent years, bio-based and biodegradable products have raised great interest since sustainable development policies tend to expand with the decreasing reserve of fossil fuel and the growing concern for the environment. Bio-Polymers are a form of polymers derived from plant sources such as sweet potatoes, soya bean oil, sugarcane, hemp oil, and corn starch. These polymers are naturally degraded by the action of microorganisms such as bacteria, fungi and algae. Bio-plastics can help alleviate the energy crisis as well as reduce the dependence on fossil fuels of our society. They have some remarkable properties which make it suitable for different applications. This paper tries to give an insight about Bio-plastics, their composition, preparation, properties, special cases, advantages disadvantages, commercial viability, its life cycle, marketing and pricing of these products.
As a result, the market of these environmentally friendly materials is in rapid expansion,
10 –20 % per year.
Natural polymers by Dr. khlaed shmareekhخالد شماريخ
the presentation is about the natural polymers i.e. classification, applications, properties and examples. it is in 25 pages in shortcuted manner and simple method.
It is a Complexaing agent.
Synonym: cavitron, cycloamyloses, cycloglucan, cyclic oligosaccharide
It is a important for increasing the solubility of poorly water soluble drugs.
Cyclodextrines are produced from starch by means of enzymatic conversion.
They are used in food, pharmaceutical, drug delivery, and chemical industries, as well as agriculture and environmental engineering.
Cyclodextrines are composed of 5 or more α-D glucopyranoside units linked 1->4, as in amylose linkage.
Cyclodextrines contains 32 1,4-anhydroglucopyranoside units, while as a poorly characterized mixture, at least 150-membered cyclic oligosaccharides are also known. Typical cyclodextrins contain a number of glucose monomers ranging from six to eight units in a ring.
CDs, with lipophilic inner cavities & hydrophilic outer surfaces, are interacting with a guest molecule to form non covalent inclusion complexes.
Today CDs are only synthesized either by fermentation or enzymatically.
Many CGTases from different microorganisms are known, cloned, sequenced, characterized and used for production of CDs.
Brief intro about crystalline and amorphous structures,
glass transition temperature,
free volume theory of glass transition temperature,
factors effecting glass transition temperature etc.
Hydrogels are three-dimensional network of hydrophilic cross-linked polymer that do not dissolve but can swell in water or can respond to the fluctuations of the environmental stimuli
Hydrogels are highly absorbent (they can contain over 90% water) natural or synthetic polymeric networks
Hydrogels also possess a degree of flexibility very similar to natural tissue, due to their significant water content
Plastics has been evolving now a days. Our lives has been filled with plastics. Almost all of our things are made of plastics but do you what it is and what it is made of?
One of the most common and widely used plastic is polyethylene or PE with the resin codes 2 and 4. It is mostly used as plastic bags, food wraps, bulletproof vest, pipes and so many more. Here is a little preview of polyethylene and what is its purpose in our daily lives.
What is polyethylene?
Its properties, structure and applications.
polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units.
Cellulose is an important structural component of the primary cell wall of green plants, many forms of algae and the oomycetes. Some species of bacteria secrete it to form biofilms.
Cellulose is the most abundant organic polymer on Earth. The cellulose content of cotton fiber is 90%, that of wood is 40–50% and that of dried hemp is approximately 45%.
Cellulose is mainly used to produce paperboard and paper.
Smaller quantities are converted into a wide variety of derivative products such as cellophane and rayon.
Conversion of cellulose from energy crops into biofuels such as cellulosic ethanol is under investigation as an alternative fuel source.
Cellulose for industrial use is mainly obtained from wood pulp and cotton.
Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms that live in their guts, such as Trichonympha.
In humans, cellulose acts as a hydrophilic bulking agent for feces and is often referred to as a "dietary fiber".
speciality polymers are the class of some high perfomance polymers ,including plastic,polymers,fluids,membranes,smart hydrogels,and elastomers that are designed to meet the critical requirement that engineers face everyday in key industries including plastic, automobiles, aeronautics, smart devices, health care, pharmacy, energy production and storage. this article is a short but diversified study of post grad. students in the area of speciality polymers, the types and applications in engineering, technology ,life sciences and research. it include diverce applications of iconic polymer metal composites liquid crystal polymers ,synthetic polymer membranes, smart hydrogels, and dendritic polymers.
Background- Chitosan is the most abundant natural amino polysaccharide. Researchers have found that chitosan is biocompatible, biodegradable and nontoxic, which have made wide applicability in the pharmaceutical field. Objectives- Aim of the study was to prepare Chitosan from chitin and characterize them. Methods- Chitosan was prepared by deacetylation of chitin and characterized by U.V Spectrophotometry, FTIR (Fourier transform infrared spectroscopy), DLS (Dynamic Light Scattering), and Scanning electron microscopy (SEM). Results- The present study showed that Chitosan was successfully prepared by deacetylation of chitin. The obtained chitosan was characterized for further study. Conclusion- Our study confirms the preparation by Chitosan from Chitin for further study. Key-words- Chitin, Chitosan, Deacetylation, DLS, FTIR, SEM
Chemical and Physical properties of Cassava Starch-Cm-Chitosan-Acrylic Acid Hydrogel prepared from radiation –induced crosslinking
Gatot Trimulyadi Rekso
Center for Application of Isotopes and Radiation- National Nuclear Energy Agency
Jl. Lebak Bulus Raya No. 49, Jakarta-Selatan, Indonesia
Corresponding author; e-mail; gatot2811@yahoo.com ,
Fax: +62-21-.7513270, HP ; 08129419442
Collagen-polyurethane-chitosan hydrogels were synthesized by modifying the chemical structure of the crosslinking agent, with the aim to test which one plays a better role in removing of lead ions from water through adsorption process. In the first instance, two chemical crosslinkers based on aqueous polyurethane prepolymers (PPU) were used, where the type of aliphatic diisocyanate: hexamethylene diisocyanate P(HDI) or isophoronadiisocyanate P(IPDI) was varied. Hydrogels were subsequently designed using type I collagen (C) and chitosan (Q) varying the type of crosslinker: CQ-P(HDI) and CQ-P(IPDI), respectively. Hydrogels were characterized by means of crosslinking index, infrared spectroscopy (FTIR), thermogravimetric behavior (TGA) and swelling/degradation kinetics. Finally, tests were performed to determine the removal rate of Pb (II) ions in model waters. The results indicate that CQ-P(HDI) hydrogels have a higher degree of crosslinking, improving its resistance to the both thermal and hydrolytic degradation, and higher swelling capacity at acidic pH; compared to those derived from CQ-P(IPDI); however, these hydrogels do not show a decrement in the removal rate of Pb (II) ions from water, compared to the CQ hydrogel (without crosslinking), thus these innovative materials could be used as an alternative with potential use in the remediation of waters contaminated with lead ions.
cellulose and chitosan belongs to polysaccharide group of carbohydrates. these two compounds are very good biopolymer and also have various applications in fabric, biomedical field etc..,
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
2. Introduction
Chitosan is a linear polysaccharide composed of randomly distributed α-(1→4)-
linked D-glucosamine ( deacetylated unit) and N-acetyl-D-glucosamine (acetylated
unit).
It is made by treating the chitin shells of shrimp and other crustaceans.
3. Chitin: a brief history
1811 Chitin was first discovered by Professor Henri Braconnot,
who isolated it from mushrooms and name it “Fungine”.
1823 Antoine Odier found chitin while studying beetle cuticles and
named “chitin” after Greek word “chiton” (tunic, envelope).
1859 Rought discovered chitosan, a derivative of chitin.
1920s Production of chitin fibers from different solvent systems.
1930s Exploration of synthetic fibers.
1950s The structure of chitin and chitosan was identified by X-ray
diffraction, infrared spectra, and enzymatic analysis.
1970s “Re-discovery” of the interest in chitin and chitosan.
1977 1st international conference on chitin/chitosan.
Henri Braconnot (1780-1856)
4. What is Chitin?
• Chitin is a natural polysaccharide.
• Structure similar to cellulose with hydroxyl group replaced by acetamino group.
• N-acetyl-glucosamine units in β-(1→4) linkage.
• Found in the exoskeleton as well as in the internal structure of shells of shrimp
and crustaceans.
• Chitin has 3 polymorphic form:
α-chitin, β-chitin, γ-chitin
α-chitin:
- the most abundant form
- anti-parallel configuration
- highly ordered crystalline
- strong H-bonding (N-
H····O=C)
- rigid, intractable, insouble.
β-chitin:
- found in diatom
spines and squid pens
- parallel configuration
- weak H-bonding
- unstable, soluble in
water.
γ-chitin:
- mixture of α and β-
chitin
- intermediate
properties.
6. 6
Deacetylation
(boiling 40-50%
NaOH)
Preparation of Chitosan
• Produced commercially by deacetylation of chitin.
• The degree of deacetylation (%DD) can be determined by NMR spectroscopy.
• the %DD in commercial chitosan ranges from 60 to 100%.
7. Properties of Chitosan-
• Unique characteristics of chitin and chitosan:
Biocompatible
Biodegradable
Non-toxic
Remarkable affinity to proteins
Ability to be functionalized
Renewable
Abundant
• Antimicrobial properties:
Mechanism still unknown hypotheses:-
binding to cell-wall phospholipids of
Gram-negative bacteria
modification of cell-wall permeability and
loss of material
inhibition of certain enzymes.
8. Determination of degree of deacetylation(DD%):
• Measured by the acid-base titration method (Domard & Rinaudo
1983) with modifications.
• Chitosan (0.1 g) was dissolved in 30 ml HCl aqueous solution
(0.1mol/l) at room temperature.
• Add 5–6 drops of methyl orange.
• The red chitosan solution was titrated with 0.1mol/l NaOH
solution until it turned orange.
The DD% was calculated by the formula:
C1 = concentration of standard HCl (mol/l),
C2 = standard NaOH solution (mol/l),
V1= volume of the standard HCl aqueous solution used to
dissolve chitosan (ml),
V2= volume of standard NaOH solution consumed during
titration (ml),
M= weight of chitosan (g)
9. Water binding capacity-
• Measured using a modified method of Knorr.
• It carried out by weighing a centrifuge tube containing 0.5 g of sample, adding 10 ml of
water and mix it on a vortex mixer for 1 min.
• Contents were left at ambient temperature (29°C) for 30 min. After the supernatant was
decanted, the tube was weighed again.
WBC was calculated as follows:
10. Fat binding capacity-
• It also measured by using a modified method of Knorr.
• Carried out by weighing a centrifuge tube containing 0.5 g of sample, adding 10 ml
of oil (soybean oil) and mixing on a vortex mixer for 1 min.
• The contents were left at ambient temperature for 30 min. After the supernatant was
decanted, the tube was weighed again.
FBC was calculated as follows:
Viscosity-
• The viscosity of chitosan increases with increasing chitosan concentration,
decreasing temperature, and increasing degree of deacetylation.
Reference- J. Bangladesh Agril. Univ. 12(1): 153–160, 2014 ISSN 1810-3030
Production and characterization of chitosan from shrimp waste
M. S. Hossain* and A. Iqbal
Department of Food Technology & Rural Industries, Bangladesh Agricultural University,
Mymensingh-2202,
Bangladesh, *Email: sajjad.bau@gmail.com
11. Moisture content-
• It absorbs moisture from atmosphere.
• Particle size distribution: <30 mm
• determined by the gravimetric method
Solubility-
• Sparingly soluble in water.
• Practically insoluble in ethanol and other organic solvents.
• Solubility is affected by degree of deacetylation.
PH: 4.0-6.0
Density: 1.35-1.40 g/cm3
Glass transition temperature: 203°C
13. Derivatives of Chitosan-
Reference- MARCIN H. STRUSZCZYK, Tricomed SA, ul' Piotrkowska 27 0, 90-950 Łódź
e-mail: martinst@skrzynka.pl Chitin and Chitosan
19. Applications and Uses-
APPLICATION EXAMPLE
Water treatment Removal of metal ions
Flocculant/coagulant
(proteins, dyes, amino
acids)
Filtration
Pulp and paper Surface treatment
Photographic paper
Carbonless copy paper
Biomedical Bandages, sponges
Artificial blood vessels
Blood cholesterol control
Tumor inhibition
Skin burns, artificial skin
Eye humor fluid
Contact lenses
Controlled release of drugs
20. APPLICATION EXAMPLE
Cosmetics Make-up powder
Nail polish
Moisturizers
Biotechnology Enzyme/cell
immobilization
Protein separation
Chromatography
Glucose electrode
Agriculture Seed/leaf coating
Hydroponic/fertilizer
Controlled agrochemicals
release
21. Biomedical Applications
• Wound dressings are used to protect
wound skin form insult, contamination
and infection
• Chitin-based wound dressings
- Increase dermal regeneration
- Accelerate wound healing
- Prevent bacteria infiltration
- Avoid water loss
• Chitin surgical threads - strong, flexible,
decompose after the heals
Chitosan wound dressings
1. Wound Dressing 2. Anticoagulation
Anticoagulation is essential for open-
heart surgery and kidney dialysis
Preventing blood from clotting during
the surgery
Sulfated chitosan derivatives have
good anticoagulant activity
22. • Tissue engineering research is
based on the seeding of cells onto
porous biodegradable matrix
• Chitosan can be prepared in porous
forms permitting cell growth into
complete tissue
3. Tissue Engineering 4. Orthopedic Applications
Bone is a composite of soft collagen
and hard hydroxyapatite (HA)
Chitin-based materials are suitable
candidate for collagen replacement
(chitin-HA composite)
Mechanically flexible, enhanced bone
formation
Temporary artificial ligaments for the
knee joint
Porous character of chitosan scaffold
50μm
23. 5. Drug Delivery
Hydrogels
• Hydrogels are highly swollen, hydrophilic polymer
networks that can absorb large amounts of water
• pH-sensitive hydrogels have potential use in site-
specific drug delivery to gastrointestinal tract (GI)
• Chitosan hydrogels are promising in drug delivery
system
Microcapsules
• Microcapsule is defined as a spherical
empty particle with size varying from
50 nm to 2 mm
• Chitosan-based microcapsules are
suitable for controlled drug release.
24. Biotechnology applications
Gene Delivery
• Viral gene delivery / Non-Viral gene delivery
• Viral: high transfection efficiency, dangerous
• Non-Viral: low transfection efficiency, safer
• Chitosan-DNA complexes can be optimized to
enhance the transfection efficiency
Enzyme immobilization
Specific, efficient, operate at mild conditions
Unstable, sensitive after isolation and purification
Chitin and chitosan-based materials are suitable
enzyme immobilizers
- Biocompatible
- Biodegradable
- High affinity to protein
- Reactive functional group
25. Reference of Applications and uses- Journal of Scientific & Industrial Research Vol.
63, January 2004, pp 20-31 Chitin and chitosan: Chemistry, properties and
applications Pradip Kumar Dutta*, Joydeep Dutta+ and V S Tripathi+ Department of
Chemistry, Motilal Nehru National Institute of Technology, Allahabad 211 004
26. ACKNOWLEDGEMENT
I wish to express my sincere gratitude to Dr. Jyoti
Pandey for providing me an opportunity to do my
presentation work on “Chitosan”
I sincerely thank Dr. Jyoti Pandey for their guidance and
encouragement in carrying out this presentation work. I
also wish to express my gratitude to my class friends of
BBAU, who rendered their help during the period of my
presentation work.