The document discusses the anti-bacterial properties of Cajanus cajan leaf extract against Streptococcus mutans. It finds that the filtered Cajanus cajan leaf extract has greater inhibitory effects against S. mutans compared to the unfiltered extract. The experiment shows that the filtered extract creates inhibition zones of 1.3-4.3mm while the unfiltered extract has no inhibition zones. This supports the hypothesis that the filtered extract would have better inhibitory effects.

1. ANTI-BACTERIAL POPERTIES OF CAJANUS
CAJAN LEAF

2. ABOUT THE PLANT
 Cajanus cajan, also called Pigeon Pea plant
 Short-term perennial shrub
 Belongs to the Fabaceae family of the Dicot
group.
 Its rich in proteins, carbohydrates and
minerals
 Medicinal applications

3. IMPORTANT INFORMATION
 Excellent source of anti-bacterial materials
 Possible antibacterial component inside the
leaf: Cajanuslactone

4. STREPTOCOCCUS MUTANS
 Inhibits the human oral cavity
 Makes 36% of the total bacteria inhibiting
human teeth
 Heterotrophic organism
 Reproduce asexually
 Facultative anaerobe

5. OBJECTIVE
 Prove if Cajanus cajan contain anti-bacterial
properties against S.mutans and if filtrating or
not the extract will affect in any way the
antibacterial potential of the extract.
Hypotesis
 The Cajanus cajan leaf extract will inhibit the
growth of S.mutans and the filtered extract will
have a better inhibitory effect than the unfiltered
extract.

6. MATHERIALS AND METHODS
Obtain extract by
Grind the leaves. adding water and Vacuum Carry out
processing it in a filtration of the aseptic
blender extract. techniques.
Make a bacterial Place the steril filter paper disc.
lawn with S. mutans Incubate the Petri dishes.

7. RESULTS

8. Table 1: Inhibition zone measurements
Measurements in mm
Trial 1 Trial 2 Trial 3
Cajanus cajan leaf extract 0.0 0.0 0.0
(unfiltered)
Cajanus cajan leaf extract 1.3 1.8 4.3
(filtered)
Control 0.0 0.9 0.0

9. Figure 1: Inhibition zone measurements
5
4.5
Size of inhibition zones (mm)
4
3.5
3
2.5 Leaf extract (unfiltered)
2 Leaf extract (filtered)
1.5 Control
1
0.5
0
Trial 1 Trial 2 Trial 3
Experimentation Trails

10. REFERENCES
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11.  Raintree Nutrition [Internet]. 2005. Carson City (NV):
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12.  Zu, Y., Liu, X., Fu, Y., Wu, N., Kong, Y., Wink, M. 2010.
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