This document summarizes research conducted by students and a professor to study sub-chemocline biofilms in Green Lake, NY using a remotely operated vehicle. The lake has distinct non-mixing water layers that support unique geochemical conditions and bacterial biofilms were discovered growing below the chemocline. Samples of the biofilms were collected using a custom ROV to identify the bacteria and understand their environment. Microscopy showed the biofilms consisted of fine filaments and sulfur particles, indicating they may be sulfide-oxidizing bacteria similar to those found in marine environments. Genomic analysis will be performed to further characterize the microbial community.