BIS2C. Biodiversity and the Tree of Life. 2014. L8. Intro to Microbial Divers...Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life.
At UC Davis Spring 2014.
Lecture 8.
Introduction to Microbial Diversity, part 2.
Slides for Lectures by Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life. 2014. L8. Intro to Microbial Divers...Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life.
At UC Davis Spring 2014.
Lecture 8.
Introduction to Microbial Diversity, part 2.
Slides for Lectures by Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life. 2014. L11. Symbioses and the Human ...Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life.
At UC Davis Spring 2014.
Lecture 11.
Symbioses and the Human MIcrobiome
Slides for Lectures by Jonathan Eisen
DNA-based methods for bioaerosol analysisjordanpeccia
Information for producing phylogenetic/taxonomic libraries of airborne bacteria and fungi. Includes fundamental background information, approaches for sequencing and data analysis, two case studies, and a review of sampling methods
BIS2C. Biodiversity and the Tree of Life. 2014. L11. Symbioses and the Human ...Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life.
At UC Davis Spring 2014.
Lecture 11.
Symbioses and the Human MIcrobiome
Slides for Lectures by Jonathan Eisen
DNA-based methods for bioaerosol analysisjordanpeccia
Information for producing phylogenetic/taxonomic libraries of airborne bacteria and fungi. Includes fundamental background information, approaches for sequencing and data analysis, two case studies, and a review of sampling methods
Innovations in Sequencing & Bioinformatics
Talk for
Healthy Central Valley Together Research Workshop
Jonathan A. Eisen University of California, Davis
January 31, 2024 linktr.ee/jonathaneisen
Thoughts on UC Davis' COVID Current ActionsJonathan Eisen
Slides I used for a presentation to Chancellor May's leadership council about the current state of UC Davis' response to COVID and how it could be improved
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
1. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Lecture 12:
Acquiring Novelty
BIS 002C
Biodiversity & the Tree of Life
Spring 2016
Prof. Jonathan Eisen
1
2. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Office Hours and Midterm
• Eisen: Today ~2:15 - 3:15
• Moore: Today 4:00 - 5:00
• Review Session
!Sunday
!1001 Geidt Hall
!6:00 - 7:30 PM
2
3. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Where we are going and where we have been
• Previous Lecture:
!11: Function
• Current Lecture:
!12: Novelty and Acquiring Functions
• Next Lecture:
!13: Human Microbiome
3
4. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Thought Questions & Main Topics
• How do organisms get new functions?
• How can we classify the ways organisms
live together?
4
5. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Key Concepts
• Lateral gene transfer
• Symbiosis
!Mutualism
!Commensalism
!Parasitism
• Phylogenetic applications
5
6. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Origin of Novelty
• How do organisms get new functions?
6
7. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Origin of Novelty
• How do organisms get new functions?
• Intrinsic (changes on the inside)
7
8. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Origin of Novelty
• How do organisms get new functions
• Intrinsic (changes on the inside)
• Extrinsic (acquired from the outside)
8
9. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Extrinsic Origin of Novelty
Extrinsic acquisition of novelty
• Sexual recombination
• Lateral gene transfer
• Interactions w/ Other Organisms
9
10. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Extrinsic Origin of Novelty
Extrinsic acquisition of novelty
• Sexual recombination
• Lateral gene transfer
• Interactions w/ Other Organisms
10
11. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 11
Sexual Recombination
In eukaryotes, the variants produced by mutation can “recombine” via sex
meiosismeiosis
fertilization
12. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Extrinsic Origin of Novelty
Extrinsic acquisition of novelty
• Sexual recombination
• Lateral gene transfer
• Interactions w/ Other Organisms
12
13. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Lateral gene transfer model
13
A CB D E F G
Note Slides Compared to
Those Used in Section A
14. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 14
A CB D E F G
Gene
Transfer
Lateral gene transfer model
15. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 15
A CB D E F G
Lateral gene transfer model
Suppose this
was EFG
Gene
Transfer
16. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 16
A CB D E F G
Lateral gene transfer model
Suppose this
was EFG
Now D will
have 2 EFGs
EFG
transfer
17. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 17
A CB D E F G
Lateral gene transfer model
EFG
transfer
18. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 18
A1 CA2 D1 E F G1D2B G2
Lateral gene transfer model
EFG
transfer
19. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 19
Suppose we built
phylogenetic trees
with EFGs form
these species
A1 CA2 D1 E F G1D2B G2
Lateral gene transfer model
EFG
transfer
20. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 20
A1 CA2 D1 E F G1D2B G2
EFG Set #1
‘Normal” EFGs from
D1, D2
21. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 21
A1 CA2 D1 E F G1D2B G2
EFG Set #1
Also any other non-
transferred genes
22. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
EFG Set #2
22
A1 CA2 D1 E F G1D2B G2
‘Transferred” EFGs for
D1, D2
23. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
All EFGs
23
A1 CA2 D1 E F G1D2B G2D1 D2
All EFGs
25. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 25
A1 CA2 D1 E F G1D2B G2
Double Lateral Transfer of EFGs
If we built a tree of all the
EFGs in these organisms,
which of the following would
not be seen as a
monophyletic grouping?
A: D1, D2
B. D1, D2, E
C. D1, D2, B
D. G1, G2, F
E. A1, A2
EFG
26. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 26
A CA2 D1 E F G1D2B G2
If we built a tree of all the
EFGs in these organisms,
which of the following would
not be seen as a
monophyletic grouping?
A: D1, D2
B. D1, D2, E
C. D1, D2, B
D. G1, G2, F
E. A1, A2
EFG
Double Lateral Transfer of EFGs
27. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 27
A CA2 D1 E F G1D2B G2
If we built a tree of all the
EFGs in these organisms,
which of the following would
not be seen as a
monophyletic grouping?
A: D1, D2
B. D1, D2, E
C. D1, D2, B
D. G1, G2, F
E. A1, A2
EFG
Double Lateral Transfer of EFGs
28. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016 28
A CA2 D2 E F G1D2B G2D1 D1 B
If we built a tree of all the
EFGs in these organisms,
which of the following would
not be seen as a
monophyletic grouping?
A: D1, D2
B. D1, D2, E
C. D1, D2, B
D. G1, G2, F
E. A1, A2
Double Lateral Transfer of EFGs
29. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Antibiotic Resistance Can Transfer Between Species
• http://www.niaid.nih.gov/
SiteCollectionImages/topics/
antimicrobialresistance/3geneTransfer.gif
29
30. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Extrinsic Origin of Novelty
Extrinsic acquisition of novelty
• Sexual recombination
• Lateral gene transfer
• Interactions w/ Other Organisms
30
31. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Symbioss
• Symbiosis is an intimate association between at least
two different organisms in which at least one of them
benefits
• Endosymbiosis is a symbiosis in which one of the
organisms live inside the cells of the other
32
32. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Classes of symbiosis
Organism
Class of symbiosis A B
Mutualism + +
Commensalism + 0
Parasitism + -
33
33. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Classes of symbiosis
Organism
Class of symbiosis A B
Mutualism + +
Commensalism + 0
Parasitism + -
34
34. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Mutualistic Symbioses of Bacteria in Eukaryotes
• Digestive
! Ruminants
! Cellulolytic insects
• Defensive
• Behavioral
! Squid light organs
• Autotrophic
! Photosynthetic (many)
! Chemosynthetic in deep sea
• Nutritional
! Aphids
! Nitrogen fixation in legumes
35
35. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Mutualistic Symbioses of Bacteria in Eukaryotes
• Digestive
! Ruminants
! Cellulolytic insects
• Defensive
• Behavioral
! Squid light organs
• Autotrophic
! Photosynthetic (many)
! Chemosynthetic in deep sea
• Nutritional
! Aphids
! Nitrogen fixation in legumes
36
More When We Discuss PAF
(Plants, Animals, Fungi)
36. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Classes of symbiosis
Organism
Class of symbiosis A B
Mutualism + +
Commensalism + 0
Parasitism + -
37
37. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Classes of symbiosis
Organism
Class of symbiosis A B
Mutualism + +
Commensalism + 0
Parasitism + -
38
More in Lecture 13
The Human Microbiome
38. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Classes of symbiosis
Organism
Class of symbiosis A B
Mutualism + +
Commensalism + 0
Parasitism + -
39
39. II. Some terms
• Pathogens are infectious agents that cause a disease
(can be considered a subclass of parasites)
• Pathogenicity = ability to enter a host and cause disease
• Virulence = degree of pathogenicity
• Note - not all parasites are pathogens but all pathogens
are parasites
!40
40. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
The Following is a Brief Tour
41
41. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Spirochetes
• Gram-negative
• Motile
• Chemoheterotrophic
• Unique rotating, axial
filaments (modified
flagella)
• Many are pathogens:
!Syphilis
!Lyme disease
• Others free-living
42
42. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Chlamydias
• Gram-negative
• Cocci or rod-shaped
• Extremely small
• Live only as parasites
inside cells of
eukaryotes & cause
various diseases
!Trachoma
!Multiple sexually
transmitted
diseases
!Pneumonia
43
C. trachomatis
43. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
High-GC Gram Positives (Actinobacteria)
• High G+C/A+T ratio in DNA
• Elaborate branching
• Some reproduce by forming
chains of spores at tips of
filaments
• Most antibiotics are from this
group
• Causative agents of many
diseases such as
tuberculosis and leprosy
• Many originally misclassified
as fungi
44
44. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Low-GC Gram Positives (Firmicutes)
• Low G+C/A+T ratio in DNA
• Some produce endospores
which are resistant “seeds”
that germinate when
conditions are good
• Many agents of diseases
(e.g., anthrax, MRSA,
Streptococcus, botulism,
tetanus)
• Many of agricultural and
industrial use (e.g., Lactic
acid bacteria)
• Some (Mycoplasmas) have
no cell wall and are
extremely small
45
Mycoplasmas
45. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Proteobacteria
• Gram-negative
• Escherichia coli: model
organism and human
gut commensal and
pathogen
• Mitochondria evolved
from this group
• Includes many human
and animal
pathogens: plague,
cholera, typhoid
46
46. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Alveolates: Apicomplexans
• All parasitic
• Have a mass of organelles at one tip
—the apical complex that help the
parasite enter the host’s cells.
47
Apical complex • Plasmodium falciparum-
Malaria kills 700,000-2,000,000
people per year—75% of them
are African children
47. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Alveolates: Ciliates
48
Movement in a ciliate from the gut of a termite
• All have numerous cilia, the structure
is identical to flagella.
• Most are heterotrophic; very diverse
group.
• Have complex body forms and two
types of nuclei.
• Some pathogens (e.g., Ick)
48. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Stramenopiles: Oomcyetes
Phytophthora
Potato Late Blight
• Non-photosynthetic.
• Are absorptive heterotrophs
• Once were classed as fungi, but
are unrelated.
49
Sudden Oak Death
49. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Excavates: Diplomonads and Parabisalids
• Unicellular
• Lack mitochondria and most are
anaerobic. This is a derived condition
• Giardia lamblia - a diplomonad - is a
human parasite
• Trichomonas vaginalis - parabasalid - STD
50
50. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Excavates: Kinetoplastids
• Unicellular parasites with two flagella and a
single mitochondrion.
• Mitochondrion contains a kinetoplast -
structure with multiple, circular DNA
molecules
• Includes trypanosomes and agents of
chagas, sleeping sickness, Leishmaniasis
Trypanosoma sp.
mixed with blood cells
51
51. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Excavates: Heteroloboseans
• Amoeboid body form.
• Naegleria can enter humans and
cause a fatal nervous system
disease - “brain eating”
• Some can transform between
amoeboid and flagellated stages.
52
52. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
• Not colonial; live as single cells
• Some secrete shells or glue sand
grains together to form a casing.
• Many pathogens
53
Amoebozoans: Loboseans
53. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
No archaeal pathogens
• Lots of types of pathogens
! Bacteria that infect eukaryotes
! Viruses that infect eukaryotes, archaea and
bacteria
! Eukaryotes that infect other eukaryotes
• No known archaeal pathogens of any organism
! No clear explanation of why
! If you discover one, you will become famous
(well, among scientists)
54
54. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Case Study: Anthrax
55
55. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014 56
Slide by Brian Moore for BIS2C at UC Davis Spring 2016
57. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Bacillus anthracis
• A member of the Firmicutes
(low GC Gram positive)
phylum
• Sporulates
• Animal and human pathogen
• Highly invasive
• “Weoponized” by multiple
countries
58
58. Anthrax forensics
• Question - How do you figure out where the
Anthrax in the letters came from?
• Answer came from phylogenetics
!60
59. Anthrax Diversity
Figure 3. Worldwide distribution of B. anthracis clonal lineages:Phylogenetic and geographic relationships
among 1,033 B. anthracis isolates.
doi:10.1371/journal.pone.0000461.g002
!61
60. Anthrax Diversity
Figure 3. Worldwide distribution of B. anthracis clonal lineages:Phylogenetic and geographic relationships
among 1,033 B. anthracis isolates.
doi:10.1371/journal.pone.0000461.g002
!61
61. Anthrax Diversity
Figure 3. Worldwide distribution of B. anthracis clonal lineages:Phylogenetic and geographic relationships
among 1,033 B. anthracis isolates.
doi:10.1371/journal.pone.0000461.g002
!61
62. Anthrax Diversity
Figure 3. Worldwide distribution of B. anthracis clonal lineages:Phylogenetic and geographic relationships
among 1,033 B. anthracis isolates.
doi:10.1371/journal.pone.0000461.g002
!61
63. VNTR Tree by Paul Keim et al
Figure 2. UPGMA dendrogram of VNTR data from worldwide B. anthracis isolates: Fifteen VNTR loci and
UPGMA cluster analysis were used to establish genetic relationships among the 1,033 B. anthracis isolates.!62
64. VNTR Tree by Paul Keim et al
Figure 2. UPGMA dendrogram of VNTR data from worldwide B. anthracis isolates: Fifteen VNTR loci and
UPGMA cluster analysis were used to establish genetic relationships among the 1,033 B. anthracis isolates.!63
65. VNTR Tree by Paul Keim et al
Figure 2. UPGMA dendrogram of VNTR data from worldwide B. anthracis isolates: Fifteen VNTR loci and
UPGMA cluster analysis were used to establish genetic relationships among the 1,033 B. anthracis isolates.!63
• The “AMES”
strain of
anthrax
• Used in labs
throughout
world
68. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Case Study: Viruses and the Tree of Life
66
69. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Viruses
Viruses are obligate parasites of other organisms and
cannot live on their own
67
70. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014 68
Viral Diversity
71. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Phage
69
A double-stranded DNA virus: Bacteriophage
T4. Viruses that infect bacteria are referred to
as bacteriophage (or simply phage). T4
attaches leglike fibers to the outside of its host
cell and injects its DNA into the cytoplasm
through its “tail” (pink structure in this
rendition).
A double-stranded DNA mimivirus: This
Acanthamoeba polyphaga mimivirus (APMV) has
the largest diameter of all known viruses and a
genome larger than some prokaryote genomes. It
is named for its host, an amoeba. Cutaway view.
150 nm60 nm
• Phage are DNA
viruses that infect
bacteria and
archaea
• Phage therapy
involves using
phage to attack
bacterial infections
• Repopularize w/
spread of
antibiotic
resistance
72. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
CRISPR is an Adaptive Immune System for Bacteria/Archaea
71
doi:10.1016/j.biochi.2015.03.025
2015 Breakthrough Prize
Jennifer Doudna
Emmanuelle Charpentier
73. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2016
Mimivirus
72
A double-stranded DNA virus: Bacteriophage
T4. Viruses that infect bacteria are referred to
as bacteriophage (or simply phage). T4
attaches leglike fibers to the outside of its host
cell and injects its DNA into the cytoplasm
through its “tail” (pink structure in this
rendition).
A double-stranded DNA mimivirus: This
Acanthamoeba polyphaga mimivirus (APMV) has
the largest diameter of all known viruses and a
genome larger than some prokaryote genomes. It
is named for its host, an amoeba. Cutaway view.
150 nm60 nm
• Mimivirus is a
DNA virus w/ giant
genome
• It is infected by its
own viruses
• Incredibly diverse
functional content
74. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Mimiviruses Genomes Similar in Size to Those of Many Parasitic
73
75. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Where do viruses sit on the tree of life?
Viruses are obligate parasites of other organisms and
cannot live on their own
74
76. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014 76
Bacteria Archaea Eukaryotes
Virus Evolution Model 1: The Fourth Domain
Viruses
77. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014 77
Bacteria Archaea Eukaryotes
Virus Evolution Model 2: Separate Origin
Viruses
78. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014 78
Bacteria Archaea EukaryotesViruses Viruses
Virus Evolution Model 3: From Within Other Groups
79. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Probably a Little of Each
79
80. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Where do viruses sit on the tree of life?
• Viruses are obligate parasites of other organisms and
cannot live on their own
• Three main theories about viruses and where they sit on
the tree of life
• 1. Viruses are relics from a pre-cellular world
• 2. Viruses are escaped portions of cellular organisms
• 3. Viruses are extremely derived and reduced cellular
organisms
80
81. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Case Study: Influenza Virus
81
82. Influenza virus
!82
A negative-sense single-stranded RNA
virus: Influenza virus H5N1, the “bird flu”
virus. Surface view.
A positive-sense single-stranded RNA
virus: Coronavirus of a type thought to be
responsible for severe acute respiratory
syndrome (SARS). Surface view.
50 nm50 nm
• “Influenza” – term dates
from 15th century Italy when
epidemics were attributed to
the influence of the stars
• Negative strand RNA
viruses
• 8 single strand
chromosomes
• Two key proteins for
antigenicity
! H = Hemagglutanin
! N = Neuraminadase
83. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Flu Phylogeny
PLoS Currents Influenza.
2009 Sep 3:RRN1031. 83
Different segments of
the flu genome can
have very different
histories
84. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Flu Recombination
84
85. Slides by Jonathan Eisen for BIS2C at UC Davis Spring 2014
Recent work of Prof. Brian Moore …
85
Viruses 2015, 7, 3310-3328; doi:10.3390/v7062773