This document provides information on biosensors. It defines a biosensor as a device that responds to the presence of a specific analyte by producing an electrical signal proportional to the analyte's concentration. It notes biosensors have three main components: a receptor, transducer, and electronics. The document discusses the different types of biosensors including electrochemical, optical, piezoelectric, and thermometric biosensors. It provides examples of applications of biosensors in food analysis, quality control, and dairy industries for detecting substances like glucose, lactose, and pathogens.
Enzyme based Biosensor for pesticide DetectionSubhasis Sarkar
The biosensors could be used for pesticides rapid detection with a good stability and repeatability. As a new analytical method, biosensor could be widely used in the determination of food contamination. Biosensor techniques based on the principle of specific biological-recognition have shown satisfactory results for environmental control, food quality monitoring and toxicity detection in recent years. All these detection methods based on biosensors were shorter time response and lower cost comparing with the traditional method, but these methods were not enough convenient to use, moreover, complex detection procedures make them unsuitable for commercial and industrial applications.
A biosensor is a compact analytical device incorporating a biological or biologically derived sensing element either associated or integrated within a physicochemical transducer
Here are some slides to discuss about biosensors and their application which we prepared in graduation.
Biosensors, Types of Biosensors, Applications of Biosensors, Nanotechnology, Nanobiosensors, Components of Biosensor, Working of Biosensor, Principle of Biosensor, Examples of Biosensor, Advantages of Biosensor, Disadvantages of Biosensor, Limitations of Biosensor, Features of a Biosensor, Calorimetric Biosensors, Potentiometric Biosensors, Acoustic Wave Biosensors, Amperometric Biosensors, Optical Biosensors, Examples of a Nanobiosensor, Lab on a chip,
Applications of Lab on a chip, Glucose Biosensor
Autofluorescence - natural emission of light by biological structures when they have absorbed light.
The most commonly observed autofluorescencing molecules are NADPG and flavins.
Generally, proteins containing an increased amount of the amino acids tryptophan, tyrosine and phenylalanine show some degree of autofluorescence.
To overcome cons of labeling for in-vivo applications mostly, naturally fluorescent proteins are used.
Biosensor is the Talk of The Day. It made possible, the conversion of yesteryear's cumbersome experiments to an easier, faster all the while improving its sensitivity and specificity. This article will help you to gain an acquaintance about it, its properties, etc.
Enzyme based Biosensor for pesticide DetectionSubhasis Sarkar
The biosensors could be used for pesticides rapid detection with a good stability and repeatability. As a new analytical method, biosensor could be widely used in the determination of food contamination. Biosensor techniques based on the principle of specific biological-recognition have shown satisfactory results for environmental control, food quality monitoring and toxicity detection in recent years. All these detection methods based on biosensors were shorter time response and lower cost comparing with the traditional method, but these methods were not enough convenient to use, moreover, complex detection procedures make them unsuitable for commercial and industrial applications.
A biosensor is a compact analytical device incorporating a biological or biologically derived sensing element either associated or integrated within a physicochemical transducer
Here are some slides to discuss about biosensors and their application which we prepared in graduation.
Biosensors, Types of Biosensors, Applications of Biosensors, Nanotechnology, Nanobiosensors, Components of Biosensor, Working of Biosensor, Principle of Biosensor, Examples of Biosensor, Advantages of Biosensor, Disadvantages of Biosensor, Limitations of Biosensor, Features of a Biosensor, Calorimetric Biosensors, Potentiometric Biosensors, Acoustic Wave Biosensors, Amperometric Biosensors, Optical Biosensors, Examples of a Nanobiosensor, Lab on a chip,
Applications of Lab on a chip, Glucose Biosensor
Autofluorescence - natural emission of light by biological structures when they have absorbed light.
The most commonly observed autofluorescencing molecules are NADPG and flavins.
Generally, proteins containing an increased amount of the amino acids tryptophan, tyrosine and phenylalanine show some degree of autofluorescence.
To overcome cons of labeling for in-vivo applications mostly, naturally fluorescent proteins are used.
Biosensor is the Talk of The Day. It made possible, the conversion of yesteryear's cumbersome experiments to an easier, faster all the while improving its sensitivity and specificity. This article will help you to gain an acquaintance about it, its properties, etc.
A presentation on biosensors and its application,all datas r mainly collected from google search,and from some books by or teachers. Hope it will help you...leave your rply,, :)
This ppt is about biosensors. A brief Introduction to biosensors, history of Biosensors, working of biosensors, usage of biosensors application of biosensors in medical and other fields
This a short and efficient presentation On Biosensor for giving presentation in the upcoming seminar....
This could be more edited further for future purposes......
Contact: arnabguptakabiraj@gmail.com
This is for the beginners level giving presentation for the first time....
this presentation is about the control and measurements of pH and red-ox potential in a fermenter or a bioreactor. there are several parameters that should be monitored in order to get the maximum productivity in a fermenter. in that few parameters are very much important to measure as well adjust to its optimum level to get the high yield.
A biosensor is an independently integrated receptor transducer device, which is capable of providing selective quantitative or semi-quantitative analytical information using a biological recognition element.(IUPAC recommendations 1999)
Professor Leland c Clark junior (1918-2005) is called the father of biosensor. The inventor of the Clark electrode, a device used for measuring oxygen in blood, water and other liquids.
Biosensors play a part in the field of environmental quality, medicine and industry mainly by identifying material and the degree of concentration present.
Biosensors are the analytical device that are used to measure the concentration of analye , these type of biosensors are made with conjugation of enzymes as a biological eliment to quantify a (bio)chemical substance / analyte are reffered to as Enzyme-probe Biosensors .
Biosensors are of many types but focusing on Enzyme biosensors there are 4 main types which are briefly described in this power point presentation .
A presentation on biosensors and its application,all datas r mainly collected from google search,and from some books by or teachers. Hope it will help you...leave your rply,, :)
This ppt is about biosensors. A brief Introduction to biosensors, history of Biosensors, working of biosensors, usage of biosensors application of biosensors in medical and other fields
This a short and efficient presentation On Biosensor for giving presentation in the upcoming seminar....
This could be more edited further for future purposes......
Contact: arnabguptakabiraj@gmail.com
This is for the beginners level giving presentation for the first time....
this presentation is about the control and measurements of pH and red-ox potential in a fermenter or a bioreactor. there are several parameters that should be monitored in order to get the maximum productivity in a fermenter. in that few parameters are very much important to measure as well adjust to its optimum level to get the high yield.
A biosensor is an independently integrated receptor transducer device, which is capable of providing selective quantitative or semi-quantitative analytical information using a biological recognition element.(IUPAC recommendations 1999)
Professor Leland c Clark junior (1918-2005) is called the father of biosensor. The inventor of the Clark electrode, a device used for measuring oxygen in blood, water and other liquids.
Biosensors play a part in the field of environmental quality, medicine and industry mainly by identifying material and the degree of concentration present.
Biosensors are the analytical device that are used to measure the concentration of analye , these type of biosensors are made with conjugation of enzymes as a biological eliment to quantify a (bio)chemical substance / analyte are reffered to as Enzyme-probe Biosensors .
Biosensors are of many types but focusing on Enzyme biosensors there are 4 main types which are briefly described in this power point presentation .
Biotechnology is challenging subject to teach and understand also..its a very interesting subject in pharmacy..all the power point is made as per your syllabus with point to point discussion.
thank you
biosensors;components,types , applications and GMO biosensorsCherry
Biosensors are devices that helps to determine the concentration of an analyte in a sample. In this ppt, the definition, components, types, applications and GMO biosensors have been described.
Biosensors are nowadays ubiquitous in biomedical diagnosis as well as a wide range of other areas such as point-of-care monitoring of treatment and disease progression, environmental monitoring, food control, drug discovery, forensics and biomedical research. A wide range of techniques can be used for the development of biosensors. Their coupling with high-affinity biomolecules allows the sensitive and selective detection of a range of analytes. We give a general introduction to biosensors and biosensing technologies, including a brief historical overview, introducing key developments in the field and illustrating the breadth of biomolecular sensing strategies and the expansion of nanotechnological approaches that are now available
Molecule selective electrode system and bio sensorMayurMarvaniya1
Electrodes designed for the detection of molecules instead of ions
Biosensor: A biosensor is an analytical device which converts the biological signal into a measurable electrical signal.
Professor Leland C Clark is the father of Biosenor. Professor Leland C Clark 1918–2005
A biosensor is an analytical device which converts a biological response into an electrical signal. The term biosensor is often used to cover sensor devices used in order to determine the concentration of substances and other parameters of biological interest even where they do not utilize a biological system directly. Biosensors have become essential analytical tools, since they offer higher performance in terms of sensitivity and selectivity than any other currently available diagnostic tool. With appropriate progress in research, biosensors will have an important impact on environmental monitoring, reducing cost and increasing efficiency. Biosensors represent a rapidly expanding field, at the present time, with an estimated 60% annual growth rate; where major focus is on health care industry. Although there use is unquestionable in the field of agri food, research, security and defence. In this paper various aspects of biosensors have been touched.
Biosensors: General Principles and ApplicationsBhatt Eshfaq
A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector.
Biosensors: are analytical tools for the analysis of bio-material samples to
gain an understanding of their bio-composition, structure and function by
converting a biological response into an electrical signal. The analytical
devices composed of a biological recognition element directly interfaced to a signal transducer which together relate the concentration of an analyte
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
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The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
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Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
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After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
DevOps and Testing slides at DASA ConnectKari Kakkonen
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Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
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Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
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Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
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The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
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https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
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3. Optimization of testing processes
4. Demo
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Execution from the test manager
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Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
2. DEFINITI
ON OF
BIOSENS
ORS.
A biosensor is a device which reponds
to the presence of a specific analyte (a
chemical substance that needs to be
measured) by producing an electrical
signal proportional to the concentration
of the analyte.
It is usually constructed from three
components: receptor, transducer and
electronics (amplification and display).
3. Lenand C. Clarck invented (1962) the Clarck
Oxygen Electrode.
A pivotal device that allows real time
monitoring of patients blood oxygen level and
made surgery safer and more successful for
millions throughout world
Generations
Lenand C. Clarck
(1918-2005).
4. Specificity: Via biosensor, it’s possible to measure
specific analytes with great accuracy.
Speed: Analyte tracers or catalytic products can be
directly and instantaneously measured.
Simpltcity: Receptor and transducer are integrated
into one single sensor & the measurement of target
analytes without using reagents is possible.
Continous Monitoring Capability: Biosensor
regenerate and reuse the immobilized biological
recognition component.
5.
6. A bioreceptor or a biorecognition element, which recognizes the target analyte
and a transducer, which converts the recognition event into a
measurable electrical signal. ( Velusamy et al.
2010)
receptors
Enzymatic
Immunological
Cellular
transducers
Potentiometric
Amperometric
Electrochemical
Optoelectric
9. AMPEROMETRIC TRANSDUCER
Based on the measurement of a steady state current produced when
a constant potential is applied by a potentiostat.
This current can be related to an electrochemical species that is
consumed or produced by the biological element…consists of a
working electrode (gold, platinum, glassy carbon, graphite or
carbon), a reference electrode (Ag/AgCl) and an auxiliary electrode,
(carbon or platinum)..
The biological element can be directly
immobilized on the electrode and is
an enzyme.
Most common.
E.g. glucose biosensor, H2O2 biosensor…
10. Potentiometric biosensors measure potential differences under zero current conditions.
Antibody-antigen binding induces a small change in the charge of the proteins that can
be potentiometrically detected.
Ion selective electrodes (ISEs) are based on potentiometric measurements and can be
used for mainly enzyme based biosensors.
The change in pH due to enzyme activity can be monitored
with a pH sensitive ISE. The potential that develops across
an ion selective membrane is measured.
The first use by Guilbault and Montalvo for urea in 1969.
More recent potentiometric devices are based on field-effect transistor (FET) devices.
(Mello and Kubota, 2007)
11. CONDUCTOMETRIC BIOSENSOR :
Measure the change in conductance between
analate and refferance.
Its sensitivity is lower than other electrochemical
biosensor.
12. OPTICAL TRANSDUCERS
Optodes (or optrodes) can employ 0n absorbance, fluorescence, phosphorescence,
polarization, and surface plasmon resonance (SPR).
The development of light emitting diodes (LEDs) allows the integration of these
small and often cheap devices into sensors.
SURFACE PLASMON RESONANCE (SPR):
SPR occurs when light is internally reflected at the interface of a material with
high
refractive index and a material with low refractive index.
An oscillating wave developed at this
interface can interact with electron packages
in the conductive layer.
The plasmon excitation energy is lost from
the reflected light and can be measured with monochromatic light that is reflected
13. Two waveforms are used for
piezoelectric biosensors.
One is a Surface Acoustic Wave (SAW) device. The two electrodes are
placed on one side of the crystal and a standing surface wave is created.
High frequencies of 30-200 MHz give the crystal a very good theoretical
sensitivity, but due to practical difficulties biosensors are mainly based on
Bulk Acoustic Wave (BAW) devices. (Leonard et
al., 2003).
14. THERMO
METRIC
TRANSDU
CERS
Thermometric biosensors measuring
the change in temperature.
(Cock et al. 2009)
Thermometric biosensors exploit the
change of heat during absorption or
evolution that occurs during
biochemical reactions.
Sensitive thermistors are used to
monitor... Enzymatic reactions are
associated with enthalpy changes.
Measurements can be improved by
co-immobilizing enzymes for signal
amplification or by using high-
protonation enthalpy buffers such as
TRIS. (Giese,
2002)
16. COMMERCIAL USE OF BIOSENSOR IN
FOOD INDUSTRY…
Suitable analalytical method for safety and quality purpose.
Determine chemical and biological contaminants.
Also in Analysis of amino acids , glucose, alcohol, flavors ,
sweetener .
Detection of allergens, toxins, additives and pathogens.
In food & fermentation process quick, cheap and safe analytical process is
needed.
17. The presence of D-amino acids in food is associated with a decrease in
protein digestibility, thus affecting the bio-availability of essential amino
acids and seriously impairing the nutritional value of the food.
(Friedman 1999)
An amperometric and a colorimetric biosensor to detect and quantify
D-amino acids selectively was constructed using DAAO from Rhodotorula
gracilis. For D-alanine concentration the range varies within of 0.2–3 mM
and 0.1–1 mM for the amperometric and colorimetric system, respectively.
(Sacchi et al. 1998)
A graphite working electrode of a screen-printed strip modified with
Prussian Blue and Nafion layers. The electrode was modified with carbon
nanotubes to enhance the signal magnitude. A fast linear response was
observed for D-alanine in the concentration range from 5 to 200 mM .
. D-Amino acids were detected in fruit juices and milk samples. The
results were in a good agreement with those obtained by capillary
electrophoresis measurements. (Wcislo et al. 2007).
18. Glucose biosensors are widely applied in the monitoring of fermentation products and in
dairy, wine, beer, and sugar industry. (Mao 2008)
Measuring of O2 consumption or H2O2 production is performed during the catalytic
reaction using the substrate of interest, e.g., analyte
Glucose could be quantified in the concentration range between 50 μM and 10 mM.
(Zhu et al. 2002)
An amperometric probe-type glucose sensor with Pt working electrode and an Ag/AgCl
reference one polarized at +650 mV. The electrode was used to determine the glucose
content in real samples. (Alp et al.2000)
A biosensor electrode consisted of a thin film of ferric hexacyanoferrate (Prussian Blue)
electrodeposited on the glassy carbon electrode with immobilized glucose oxidase on a
Nafion polymer layer.Used for glucose in soluble coffee. (Mattos and Areias
2005)
19. Using different transducers based on five various Solid Binding Matrices (SBMs). The
glucose biosensors based on the transducer with cholesteryl myristate SBM and ferrocene
mediator were used for determination of glucose in wine sample. (Svorc et al.
1997)
Screen-printed electrodes to simultaneously detect D-glucose and L-lactate. They
immobilized glucose and lactate oxidase on a carbon working electrode. Ferricyanide ions
(electrochemically oxidized at a low voltage) were chosen as a mediator.
A linear range was found over a range of 1–100 mM (D-glucose) and 1–50 mM (Llactate).
Biosensor was applied after fermentation with lactic acid bacteria and with high-
performance liquid chromatography (HPLC). Method were completed within 5 min (Sato
and Okuma 2006).
Immobilized enzyme reactor (IMER) and integrated it to a capillary electrophoresis (CE)
microchip.
Glucose was detected above 100 μM range using particles modified with glucose oxidase
packed at the end of the separation channel.
The applied procedure involved the separation of the target analyte by a CE, which is then
coupled to a post-column IMER that produced H2O2, which was finally detected at the surface
of a working electrode. The microchip-CE-IMER was used to quantify glucose in carbonated
beverages. (Blanes et al. 2007)
20. ANALYSIS OF TEA BY USING
BIOSENSOR
Tea polyphenols are gaining importance due to their strong
antioxidant properties for nutrition and health.
In this context cftri, mysore successfully developed an enzyme
based amperometric biosensor for the determination of total
polyphenol content in tea infusions.
Both lab and industry trials were satisfactory
for tea polyphenols detection and tea biosensor
technology.
(Sujith Kumar et al., 2011)
21. Conventional method- severel steps…time
consuming & laborious…
Specific antibodies can be produced against
surface antigents of microbs.
Mostly for confirming the absence of
pathogen.. salmonella & e. coli… causes
bloody diarrhea, renal failure, meningitis.
Detects within minutes of sampling…
22. The conductivity of the medium changes if micro organism
metabolize the substrate (e.g. carbohydrate) to intermediate
(e.g. lactic acid). By measuring the conductivity the micro
organism growth can be determined.
(Mello and Kubota, 2002)
If pathogens are found with on- or near-line biosensors, then
food processors can make decisions more quickly about
applying treatments, minimizing the chance of a contaminated
final product. (Velasco and
Mottram, 2003)
Very specific antibodies can be produced against surface
antigens of various microorganisms.
In this way, an immunosensor can discriminate between
different organisms.
In combination with different transducers (e.g. piezoelectric
materials or optical fibers) antibodies have been successfully
employed for the detection of microorganisms.
Generally salmonella and Escherichia are detected by this
operation. (Kuhnert et al.,
2000)
23. Flow Injection Potentiometric system has been
developed for simultaneous determination of
ascorbic acid with other parameters.
This system is based on the reaction of the species with ascorbate oxidase
which is immobilized on alkaline glass beads
using glutaraldehyde. Fall in oxygen
consumption is detected by the electrode.
Oxygen consumed is proportional to the
ascorbic acid content of the sample.
(Eshkenazi et al., 2000)
24. QUALIT
Y
CONTRO
L OF
MODIFI
ED
ATMOSP
HERE
PACKAG
ES
Low package O2 also may promote growth of
dangerous pathogens (e.g. Clostridium
botulinum)… quality loss and eventually
product breakdown
Detection of ethanol provide a sensitive
technique for low-O2 injury
Commercial ethanol biosensor (with
chromagen) : Alcohol oxidase causes
oxidation of ethanol into acetaldehyde and
H2O2…peroxidase causes oxidation of the
chromagen …causing a colour change
The biosensor detects ethanol to levels below
the human olfactory threshold (l0µl/l)
ethanol in gas phase at 50°C with a 15 s
exposure. The onset of low O2 injury was
detected in lightly processed lettuce,
cauliflower, broccoli und cabbage modified
atmosphere packages as measured by
accumulation of headspace ethanol.
(Smyth et al., 1996).
25. Electronic toungue - A nanoparticle films with
embedded sensors in packaging in order to detect
pathogens. The technology will be able to detect and
alert the consumers if the food is contaminated by
triggering a colour change in the packaging.
(Selke, 2008)
Machine vision technology has a hyperspectral
imaging ability which offer the additional benefits of
sensing the chemical composition of foods. By
employing high throughput chemometrics.
(Headwall photonics,2009)
26. FISH
FRESHNE
SS
AND
MEAT
QUALITY
Fish freshness has been evaluated chemically and
expressed as K-value
K-value is calculated from the concentrations of
inosine 5-monophosphate(IMP), inosine and
hypoxanthine (Hx)…with several kinds of
biochemical reagents & using human smell sense.
(Mitsubayashi et al., 2004).
A four electrode array attached to a knife which can
be inserted into meat to measure the glucose
gradient immediately below the surface.
27. APPLICATI
ON OF
BIOSENSO
RS IN
DAIRY
INDUSTRI
ES
On line lactose measurement… a multi-enzymatic
amperometric biosensor for lactose in fresh raw milk
Microbial biosensor based on thick film technology for free
fatty acids…oxygen uptake by respiratory activity of the
immobilized microorganisms.
having a short response high sensitivity and easy handling.
ONLINE
MEASUREMENT…
28. FOR
QUALIT
Y
CONTRO
L IN
MILK &
MILK
PRODUC
TS
Urea biosensor is immobolized urease
yielding bacterial cell biomass Bacillus
sphaericus isolated from soil and coupled to
the ammonium ion selectively electrode of a
potentiometric transducer.
Response time as low as two minutes
To control the acidity of mozzarella cheese,
biosensor has been used to measure the
lactic acid.
An electrochemical (flow-through flow-jet)
cell assembled with platinum sensor covered
with the immobilized lactate oxidase
enzyme connected to an amperometer.
The amount of lactate in the curd is
detected by H2O2 probe. (Rajasekhar et al.,
2005)
29. Lactose is hydrolyzed by the enzyme β-galactosidase to galactose and glucose.
Galactose can be further oxidized to galacto-hexodialdose:
Amperometric lactose biosensor by immobilizing galactosidase and galactose oxidase
in Langmuir–Blodgett films of poly 3-hexyl thiophene/ stearic acid. The enzyme
electrodes showed linearity in the range 1–6 g dl ˉ ¹ for lactose and had a shelf life
more than 120 days...used in food and biological fluids.
(Sharma et al. 2004)
β-galactosidase immobilized into polyelectrolyte membranes with the use of
organic solvents and perfluorosulfonated polymer.
The results of the analysis of milk whey in a flow-injection system that
included lactose biosensor, based on Berlin blue (as a signal
transducer) and polyelectrolyte membranes correlated well with
measurement data obtained by a standard chromatographic
technique. (Lukacheva et al. 2007)
ANALYSIS OF LACTOSE
CONCENTRATION
30. The CDH(cellobiose dehydrogenase )-lactose sensor was successfully
used to quantify the content of lactose in pasteurized milk, buttermilk,
and low-lactose milk, using the standard addition method.
(Stoica et al.
2006)
The bioelectrode based on the use of a 3-mercaptopropionic acid self-
assembled monolayer modified gold electrode.
Beta-galactosidase catalyzed the hydrolysis of lactose, and the
produced glucose was catalytically oxidized to gluconic acid and H2O2,
which was reduced in the presence of peroxidase.
The biosensor showed a useful lifetime of 28 days and was applied to
the determination of lactose in milk and other foodstuffs (chocolate,
butter, margarine, yogurt, cheese, and mayonnaise), and the results
obtained were validated using a commercial enzyme test kit.
(Conzuelo et
al. 2010)
31. The lactate level is an indicator of the fermentative
processes and is related to the freshness, stability, and
storage quality.
Lactate determination by biosensors is typically based on
those reactions.
An amperometric lactate biosensor based on a conducting
polymer, poly-5,20-50,200terthiophene-30-carboxylic acid
(pTTCA), and multiwalled carbon nanotube (MWNT)
composite present on a gold electrode. LDH and the
oxidized form of nicotinamide adenine dinucleotide (NAD+)
were subsequently immobilized onto the pTTCA/MWNT
composite film. The detection signal was amplified by the
pTTCA/ MWNT assembly with immobilized enzyme.
(Rahman et al. 2009).
Biosensors were applied in the determination of lactate in
wine and beer, and results were in an agreement with those
obtained by an enzymatic-spectrophotometric assay kit
(Parra et al. 2006).
32. A glassy carbon electrode modified with laponite/chitosan
hydrogels for LOx immobilization. Ferrocenemethanol was
utilized as an artificial mediator. The biosensor showed a
very short response time lower than 5 s and a detection limit
of 3.8 μM. (Zanini et
al.2011)
Ballesta-Claver et al. prepared a chemiluminescencebased
one-shot biosensor tested for the analysis of lactate in
yoghurt.
The lactate recognition system was based on LOx and
the transduction system consisted of luminol, peroxidase from
Arthromyces ramosus, all immobilized in a poly-ion complex
membrane on a metallic aluminum electrode.
The performance of biosensor was validated by the
results obtained using an enzymatic reference procedure.
(Ballesta-Claver et al. 2008)
33. COMPANY ANALYTE RANGE STABILITY
Yellow Springs Instruments Glucose, lactate, ethanol 1-45, 0-15, 0-60 300 times
ZFWG, Berlin, Germany Glucose, Uric Acid 0.5-50, 0.1-1.2 >1000 times
Abbott,USA Glucose, Insulin 20-500
Roche, Switzeland Lactate, glucose, cholesterol 0.5-12, 20-600
Elite glucometer lactate 0.8-23
Nova diabetes glucose
Germaine laboratories, USA hemoglobin
Arkay, japan glucose 10-600
Pts, diagonostic, china cholesterol
Nova biomedical, USA glucose 20-600
34. Highly specific
Independent of factors like PH, stirring
Lenear response, tiny and biocompitable
Easy to use, durable
Require only small sample volume
Rapid, accurate, stable and sterilizable.
ADVANTA
GES…
36. On-site and on-line
monitoring is make
possible for biosensor
within few minutes.
Although Biosensor
concepts are a vast area of
research that continues to
develop rapidly.
It has a promising bright
future in food and
medicinal industry.
37. Bergann T., Gifley K. and Abel P., 1999.Concentration of lactic acid in carcasses
and fresh meat estimation with an enzymatic-biosensors or measuring system.
Fleischwirtschaff, 79: 84- 487.
Eshkenazi, Maltz E., Zio B. and J. Rishpon., 2000. Three cascaded enzymes
biosensor to determine lactose concentration in raw milk. J. Dairy Sci. 83(9):
1939–1945.
Girta F., 1997. Use of on-line biosensors in food industry. J. of Qafqaz University,
1(1):138-150.
Glaser R. W., 2000. Surface Plasmon Resonance Biosensors and their applications,
Kiuwer Academic/Plenum Publishers, New York.pp.195-212.
Kumar Sujith., 2011. enzyme based amperometric biosensor for the determination
of total polyphenol content in tea infusions. Journal of food science and technology.
144-152