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Biophysical Properties - Lecture - 2.pptx
- 1. Biophysical Properties –Lecture -2 Learning Objectives: Students will be able to: • Define Buffers • Enlist Classification of Buffers • Composition of Buffers • Buffers Action Mechanism & Capacity Professor Dr. Syed Irfan Raza PhD Biochemistry
- 2. B u ff e r s pH of a solution can be easily changed by the addition of acids or base
- 3. Solutions which resistchange in pH by the addition of acid or base are called buffers
- 4. Buffer Buffer Wk. acid Conj. Base Buffer Composition Amixture of a weak acid and its conjugate base or a mixture of a weak base and its conjugate acid is called a buffer.
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- 6. Acidic buffer: It isthe solution of mixture of the weak acid and a salt of this weak acid with a strong base. For Ex: CH3COOH + CH3COONa or HCOOH + HCOONa Basic buffer: It is the solution of a mixture of weak base and a salt of this weak base with a strong acid. For Ex: NH4OH + NH4Cl or NH4OH + NH4NO3
- 7. A conjugate acidis formed when a proton is added to a base. A conjugate base is formed when a proton is removed from an acid. Define Conjugate acid & Base
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- 9. 3. In asolution of HCl & Water: a. HCL will behave as a weak acid b. Water will behave as Conjugate base c. H3O+ will work as acid d. Cl- will behave as conjugate acid e. H2O will work as base
- 12. Mechanism of BufferAction
- 13. • Buffer capacity(β) is defined as a quantitative measure of the resistance of pH change when ions are added in Buffer solutions. • It is also known as acid neutralizing or alkalinity capacity. • Also it is defined as the moles or grams equivalent of an acid or a base that is required to bring a change in the pH of a solution by 1. • It depends on the concentration of a solution. Buffer Capacity (β)