This document defines key concepts in experimental design including: analysis of variance which partitions total variation into components measuring different sources of variation; treatments which are procedures whose effects are measured and compared; design of an experiment which is a plan to obtain information to answer research questions; response which is the observed result for an experimental unit; levels which are the various values or classifications of factors; experimental error which is random variation beyond human control; randomization principle which involves assigning treatments to units by chance; replication principle which refers to the number of times a treatment appears in experimental units; and local control which is a technique to reduce error by meaningfully grouping experimental units.