This document summarizes solutions for working with DNA/RNA, including isolation, preparation, reaction setup, amplification, separation, detection and analysis. It outlines automated workflows using magnetic beads and robots for nucleic acid extraction, quantification and normalization from various sample sources. Reaction setup and cleanup can be automated using robotic platforms. Separation and detection techniques mentioned include sequencing, microarrays, qPCR and next generation sequencing. Contact information is provided for more details.

1. Total Solutions for
Working with DNA/RNA
SlideShare
Luc Van Laer, Product Manager

2. Our Partners

3. Nucleic Acid Analysis Workflow
Analyse /
DNA / RNA Isolation / Separation /
Rxn Set-up Amplification Evaluate
Source Preparation Detection
Results
Human Magnetic Beads Sequencing SPRI Next Gen Candidate Genes
Animal (Magtration) Gene Expression Magnetic Beads Sequencing Signature Genes
Plant (Affinity beads) Capillary sequencing SNPs
Microbes (SPRI) Genotyping Mutations
DNA Fingerprinting Micro-Array LOH
DropSense qRT-PCR
Mutation Detection Indels
Normalise DNA DNA Sequence
Microsatellites

4. Extraction of Genomic DNA
• Our Solutions for Extraction
– Dedicated robots for batch processing
(PSS Magtration automation)
DNA / RNA Isolation /
Source Preparation • Small sample volumes (100 – 1000 l)
• Large volumes of blood (5 – 7 ml) for DNA banking
– Immunoprecipitation systems (Abnova) for Chromatin or
Human Magnetic Beads RNA immunoprecipitation
Animal (Magtration)
Plant (Affinity beads)
– Fully automated extraction platforms with Beckman
Microbes (SPRI) Coulter Biomek and Agencourt SPRI magnetic beads
DropSense • Our Solutions for Quantification
Normalize – Trinean DropSense
• Our Solutions for Normalization
– Beckman Coulter Automation

5. Automated Reaction Set-up
Our solutions for Automated Set-up
Rxn Set-up • Set your reactions up with the Biomek robotic platforms
• Verify and calibrate your robotic dispenses with Artel’s
MVS calibration systems
Sequencing
Gene Expression
Genotyping
DNA Fingerprinting
Mutation Detection
Microsatellites

6. Cleanup after amplification
• Use industry leading chemistry for your
Amplification
amplification cleanup
– AMPure XP for PCR Cleanup
– RNAClean XP for cleanup after cDNA synthesis or IVT
SPRI – CleanSEQ for SANGER sequencing cleanup
Magnetic Beads
Biomek NX-MC Biomek FX-MC

7. Separation / Detection
• Our solutions are compatible with all major
downstream technologies
• Capillary Sequencing
Separation /
Detection
• Micro-Array
• qRT-PCR
• Next Generation Sequencing SPRIWorks
Next Gen
Sequencing
Capillary sequencing
Micro-Array
qRT-PCR

8. For more information
contact us
Luc Van Laer
Analis Gent
lvl@analis.be
09/243 77 14