This document summarizes a saliva DNA isolation kit from Norgen Biotek Corp. that uses magnetic bead technology. It can isolate high-quality DNA from both fresh and preserved saliva samples. The isolation process involves a proteinase K incubation followed by binding of DNA to magnetic beads while removing proteins. This results in high yields of intact genomic DNA suitable for downstream applications like PCR, microarrays, sequencing, and genotyping.
Overcome the challenges of Nucleic acid isolation from PCR inhibitor-rich mic...QIAGEN
This presentation will focus on nucleic acid extraction tools developed by QIAGEN that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.
Advancing Microbiome Research: From challenging samples to insight with Confi...QIAGEN
Microbiome research encompasses sample types as diverse as the human gut, Antarctic soil, ocean water and acidic hot spring biofilms. These samples are challenging because they are difficult to lyse, with some microbes containing a tough extracellular matrix. Incomplete lysis of a microbial community results in an inaccurate representation of the microbial content of the sample. Additionally, PCR inhibitors present in these samples, especially humic acids, polysaccharides, polyphenolics, lipids and heavy metals result in inaccurate quantification of nucleic acids that may inhibit downstream applications such as qPCR and NGS.
Overcome the challenges of Nucleic acid isolation from PCR inhibitor-rich mic...QIAGEN
This presentation will focus on nucleic acid extraction tools developed by QIAGEN that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.
Advancing Microbiome Research: From challenging samples to insight with Confi...QIAGEN
Microbiome research encompasses sample types as diverse as the human gut, Antarctic soil, ocean water and acidic hot spring biofilms. These samples are challenging because they are difficult to lyse, with some microbes containing a tough extracellular matrix. Incomplete lysis of a microbial community results in an inaccurate representation of the microbial content of the sample. Additionally, PCR inhibitors present in these samples, especially humic acids, polysaccharides, polyphenolics, lipids and heavy metals result in inaccurate quantification of nucleic acids that may inhibit downstream applications such as qPCR and NGS.
Addressing the Pre-PCR Analytical Variability of FFPE SamplesCandy Smellie
Despite technical advances, assessing the accuracy of pre-PCR steps, which include DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissues, DNA quantitation and DNA quality control, remain a key challenge in external quality assurance.
In the webinar we will discuss the latest results from recent studies and look at ways that the accuracy of pre-PCR workflows can be improved.
A brief introduction to amplicon sequencing of the 16S rRNA gene for the analysis of microbial diversity. This talk was presented originally at the Workshop: Introduction to Systems Biology, Aalborg Denmark. 2013-10-29
As you know, Sample preparation is a relevant step to get the success in your research. For this reason, Canvax™ Extraction kits are designed for a reliable, easy and fast purification of high-quality and high-purity genomic DNA/RNA from a wide range of starting materials, like Blood, Buccal Swab, Buccal Saliva, Tissues, Cultured cells, Stool samples, Plant tissues, Soil samples, Bacteria, Yeast, Plasmid, PCR mixtures, Agarose gel slices or Serum.
Our breakthrough and proprietary technologies HigherPurity™, CleanEasy™ and WideUSE™ improves the most common Extraction methods conferring the obtained DNA and RNA a proven optimal performance for all downstream applications, such as PCR, qPCR, NGS, Cloning, STR Analysis or Gene Expression.
Automated DNA purification from diverse Microbiome samples using dedicated Mi...QIAGEN
This application note demonstrates the automation of QIAGEN’s new line of DNA sample prep kits for the microbiome. The microbiome of samples as diverse as soil, water and stool was purified using dedicated QIAcube compatible kits. Automation on the QIAcube enabled efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, the CLC Microbial Genomics Module was successfully employed for metagenome sequencing and identification of microbial composition and diversity.
Addressing the Pre-PCR Analytical Variability of FFPE SamplesCandy Smellie
Despite technical advances, assessing the accuracy of pre-PCR steps, which include DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissues, DNA quantitation and DNA quality control, remain a key challenge in external quality assurance.
In the webinar we will discuss the latest results from recent studies and look at ways that the accuracy of pre-PCR workflows can be improved.
A brief introduction to amplicon sequencing of the 16S rRNA gene for the analysis of microbial diversity. This talk was presented originally at the Workshop: Introduction to Systems Biology, Aalborg Denmark. 2013-10-29
As you know, Sample preparation is a relevant step to get the success in your research. For this reason, Canvax™ Extraction kits are designed for a reliable, easy and fast purification of high-quality and high-purity genomic DNA/RNA from a wide range of starting materials, like Blood, Buccal Swab, Buccal Saliva, Tissues, Cultured cells, Stool samples, Plant tissues, Soil samples, Bacteria, Yeast, Plasmid, PCR mixtures, Agarose gel slices or Serum.
Our breakthrough and proprietary technologies HigherPurity™, CleanEasy™ and WideUSE™ improves the most common Extraction methods conferring the obtained DNA and RNA a proven optimal performance for all downstream applications, such as PCR, qPCR, NGS, Cloning, STR Analysis or Gene Expression.
Automated DNA purification from diverse Microbiome samples using dedicated Mi...QIAGEN
This application note demonstrates the automation of QIAGEN’s new line of DNA sample prep kits for the microbiome. The microbiome of samples as diverse as soil, water and stool was purified using dedicated QIAcube compatible kits. Automation on the QIAcube enabled efficient and reliable use of these samples for sensitive downstream applications such as qPCR and NGS. In addition, the CLC Microbial Genomics Module was successfully employed for metagenome sequencing and identification of microbial composition and diversity.
It contains information about- DNA Sequencing; History and Era sequencing; Next Generation Sequencing- Introduction, Workflow, Illumina/Solexa sequencing, Roche/454 sequencing, Ion Torrent sequencing, ABI-SOLiD sequencing; Comparison between NGS & Sangers and NGS Platforms; Advantages and Applications of NGS; Future Applications of NGS.
Semi Automated Low-throughput Workflow for Microbial Analyses of Human StoolQIAGEN
The gut microbiota composition changes dramatically throughout aging and disease. A healthy gut microbiota is typically characterized by large bacterial taxonomic diversity and functional capacity, whereas frailty and aging are associated with loss of diversity and expansion of more pathogenic bacterial species. However, in order to accurately profile changes in microbial communities, the reproducible isolation of high-quality DNA is an important step. Automation enables reliable and reproducible isolation of DNA of superior quality, which can be used directly for downstream sequencing applications.
This webinar focuses on the development of a semi-automated workflow to profile the gut microbiota of young and old individuals and identify changes in bacterial composition and function that occur with age. This workflow will help to simplify and streamline the DNA extraction process for samples with high inhibitor content and subsequent microbial community analyses.
1. Figure 1. Resolution of DNA Isolated from Preserved
Saliva Samples.
High Throughput
Saliva DNA Isolation Kit
NORGENBIOTEK CORP.
The Sample Preparation Experts
T 1-866-NORGENB
T 905-227-8848
F 905-227-1061
E info@norgenbiotek.com
www.norgenbiotek.com
ISO 13485:2003, ISO 9001:2008,
ISO 15189:2012 Registered
Magnetic Bead System — Automation
Saliva DNA can either be isolated from saliva samples
collected and preserved using Nor-gen’s Saliva Collec-
tion and Preservation Devic-es or fresh saliva samples.
After a brief Protein-ase K incubation, Magnetic Bead
Suspensions are added to the sample and then placed
on the magnetic separation rack.
Only the DNA will bind to the magnetic beads, while
most of the proteins will be removed in the superna-
tant. The bound DNA is then washed in order to re-
move any remaining impurities, result-ing in a high
quality DNA elution.
DNA
Microarray
SNP Analysis
Next-Gen
Sequencing
Genotyping
Application Ready DNA
Column
(Cat. RU45400)
M A B C D E F M
High Integrity DNA
Before After
Isolate high quality and quantity DNA from fresh,
frozen or preserved saliva samples
Fast and easy processing using a magnetic bead
system
High Integrity DNA (up to 50kb)
Consistent, high yields of genomic DNA
Product Features
2. High Throughput
Saliva DNA Isolation Kit
Ordering Information
Figure 3. High Quality DNA Confirmed by Real-time PCR.Figure 2. Comparison of Saliva DNA Isolation Methods
(Column vs. Magnetic Bead System)
v3.0
0
10
20
30
40
50
60
70
Column A B C D E F
Concentration(ng/μL)
Description Prep Size Cat. Number
Saliva DNA Isolation Kit (Magnetic Bead System) 50 preps RU55400
Saliva DNA Isolation Kit 50 preps RU45400
Saliva Collection and Preservation Devices 50 units RU49000
Hierarchical Clustering Dendrogram
NORGENBIOTEK CORP.
The Sample Preparation Experts Manufactured in our ISO accredited facility.
ISO
9001:2008
ISO
15189:2012
ISO
13485:2003
Preserved human saliva samples Preserved human fecal samples
Figure 4. Hierarchical clustering of samples based on genus-level classifications, including a bar chart showing the relative
abundance of genus-level classifications for each sample in the dendrogram.
Consistent Isolation of High Quality DNA
Comparable Concentration vs.
Column-based methods
