While the use of the Sciclone NGS Workstation simplifies library prep by offering increased throughput and uniformity and decreased costs, it can also be a limiting factor if the latest library prep technology is not incorporated into the kits which are automated on the platform. Bioo Scientific has developed automation protocols for the Sciclone NGS and NGSx for a number of the NEXTflex library prep kits, so that the latest innovations available to reduce bias and increase library quality are now easily available for use with the Sciclone. With automation protocols and user guides available, these rapid and robust library prep solutions also are easy to deploy and greatly increase the end user’s multiplexing capabilities.

1. IMPROVING NGS LIBRARY
PREP AUTOMATION
on the
SCICLONE NGS WORKSTATION

2. Typical NGS workflows offer challenges
with respect to throughput,
cost & uniformity

3. Use of automated liquid handling
workstations can provide a cost-
effective solution to many of
these workflow challenges

4. Rapid & robust workflows which are
easy to deploy are critical for success

5. How is Bioo Scientific improving library
prep automation on the Sciclone?

6. EASY DEPLOYMENT

7. Bioo Scientific has developed & validated a
number of automated Illumina-compatible
library prep programs for use on the Sciclone
NGS and NGSx Workstations including
RNA-Seq, Small RNA-Seq & DNA-Seq

8. RAPID & ROBUST WORKFLOWS

9. The NEXTflex RNA-Seq, Small RNA-Seq &
DNA-Seq Kits automated on the Sciclone all
incorporate fast, user-friendly protocols

10. INCREASED MULTIPLEXING
CAPABILITIES

11. Up to 384 unique, single index
barcodes are available for deep
multiplexing of libraries

12. REDUCED RNA-SEQ BIAS

13. Bias-reducing Molecular Indexes allow
for more accurate resolution of duplicates
introduced during PCR amplification step of
stranded RNA-Seq library construction on
the Sciclone NGS or NGSx

14. Absolute Quantitation
for RNA-Seq
Molecular Indexes are included in the
NEXTflex™ Rapid Directional qRNA-Seq™ Kit
®
DOWNLOAD MANUAL VIEW SLIDESHARE

15. REDUCED SMALL RNA-SEQ BIAS

16. Ligation bias causes inconsistencies in
small RNA-Seq analysis. The incorporation
of randomized adapters during library
prep results in small RNA libraries with a
dramatic reduction in ligation bias.

17. IMPROVED LIGATION EFFICIENCY

18. The NEXTflex library prep kits automated
on the Sciclone all incorporate Bioo
Scientific’s Enhanced Adapter Ligation
Efficiency Technology

19. Which increases library diversity &
complexity, thus allowing for use of
degraded or smaller amounts of input

20. OPTIMIZED FOR AUTOMATION

21. The NEXTflex library prep kits include
master-mixes optimized for automation

22. Download the Sciclone NGS and NGSx
Workstation Automation Guides:
® ®
™
Small RNA-Seq Kit v2
Automation Guide
(Illumina® Compatible Libraries on Sciclone NGS Workstation)
®®®
NEXTflex™ Rapid Directional
qRNA-Seq™ Kit Automation Guide
NEXTflex™ Rapid Directional
RNA-Seq Kit Automation Guide
NEXTflex™ Rapid RNA-Seq
Kit Automation Guide
NEXTflex™ Rapid DNA-Seq
Kit Automation Guide
NEXTflex™ Small RNA-Seq Kit v2
Automation Guide
DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL

23. Or contact one of our Automation Specialists
today to improve your automated NGS library
prep using the Sciclone NGS Workstation

24. If you have any questions email us at
nextgen@biooscientific.com
or visit our website at
BiooScientific.com/Sciclone