Bio-analytical research scientist

1. Aubrey Perrine
4713 Hazel Avenue, Philadelphia, PA 19143
(908) 894-4376
aperrine@mail.usciences.edu
Professional Passionate bio-analytical scientist with pharmaceutical/biotech industry experience,
Summary particularly in the area’s of protein characterization using LC-MS/MS. Demonstrated ability
working in a cross-functional team where individual contribution is key.
Industry
Skills
Experience Scientist I, Biophysical Characterization
Oncobiologics, Cranbury, NJ
June 2016 – Present
• Characterize primary and secondary structures of monoclonal antibodies.
• Peptide mapping of biological therapeutics utilizing LC-MS/MS
• Sample preparation and analysis of mAb glycan species using fluorescent labeling and detection by
UPLC
• Method development and validation for protocols used within quality control
• Maintain diligent records of all lab experiments using electronic laboratory notebook (eLN)
• Proficient in generating data that is reliable, accurate, and precise.
Biologics and Vaccine Analytical Development Intern
Merck & Co., West Point, PA
June 2015 – August 2015
• Analysis of the varicella vaccine to establish possible correlation of particle size to infectivity
• Maintenance of MRC-5 cells
• Evaluate potency in various vaccine samples by quantitating plaque forming units (PFU)
• Quantitate sub-visible particulates in vaccine samples with the use flow microscopy
• Explore and locate trends in particle size to aid in vaccine characterization
• Collaborate with a diverse group of scientists to compile and organize data into a coherent report for
future evaluations
Independent Student Researcher
University of the Sciences, Philadelphia, PA
August 2014 – May 2016
• Extend previously conducted research into the mechanism of hepatotoxicity caused by the TZD,
Troglitazone...
• Organic synthesis of compounds with a similar structure to Troglitazone, such as 5-(4-
methoxyphenyl)-2,4-thiazolidinedione (MPMT) and analogs differing in benzene ring substituents.
• Analysis of 3-(3,5-dichlorophenyl)-2,4-thiazolidinedione (DCPT), a TZD ring-containing compound,
and metabolites to observe cytotoxicity
• Use of the MTS assay and the luciferase assay, to determine cytotoxicity and ATP depletion,
respectively
• Maintenance of human hepatoma HepG2 cells
• Results used to provide insight into hepatotoxicity mechanism of action
• Analyze and compile data for presentation to peers and colleagues
• Mammalian cell culture
• Pharmacokinetics
• Cell Proliferation Assays
• Cytotoxicity assays
• Plaque-based assays
• Animal Handling
• Tissue Staining
• Excellent Communication
• LC/MS
• HPLC, UPLC
• Peptide Mapping
• Particle Analysis
• Method Development
• Method Validation
• cGMP
• Microsoft Office

2. Education Bachelor of Science in Pharmacology and Toxicology
University of the Sciences
Philadelphia College of Pharmacy
Department of Pharmaceutical Sciences
May 2016, Cum Laude
GPA: 3.5
Presentations:
• “Effect of 3-(3,5-Dichlorophenyl)-2-4-thiazolidinedione (DCPT) and its Metabolites on Cell
Viability and ATP Content in HepG2 Cells” presented at the annual “Research Day
Symposium” at University of the Sciences, April 2016
• “Seamless Gene Correction of β-thalassemia Mutations in Patient-specific iPSCs using
CRISPR/Cas9 and piggyBac: A Review” presented to a student and faculty panel at
University of the Sciences, November 2015
• “Effect of Different Phenyl Substituents on 5-(4-Methoxyphenylmethylene)-2,4-
thiazolidinedione (MPMT-I) Cytotoxicity in Human Hepatoma HepG2 Cells” presented at
the annual “Research Day Symposium” at University of the Sciences, April 2015
Honors G. Victor Rossi Scholarship
Awarded to one Pharmacology and Toxicology student annually. Recipient must demonstrate
both outstanding academic performance, as well as independent research performance.
Johnson & Johnson Award for Innovation in Pharmacology and Toxicology
Awarded to the Pharmacology and Toxicology graduate with the highest GPA in the program.
Related
Courses
• Pharmacology
• Biochemical Pharmacology
• Principles of Toxicology
• Medicinal Chemistry
• Molecular Biology & Genetics
• Biochemistry
• Organic Chemistry
• Physiology
• Anatomy & Histology
• Immunology