The formation of Li-Fe antisite defects was observed in Li2FePO4 cathode material upon electrochemical cycling. It was revealed, that oxygen atoms, which are coordinated by electrochemically active Li atoms, induce the formation of defect. This work was published in Chem. Mater., 2016, 28 (21), pp 7578–7581 DOI: 10.1021/acs.chemmater.6b03746