This presentation details antimicrobial validation capabilities. It is presented by Mérieux NutriSciences, the leading internationally accredited provider of food safety, quality and nutrition services. In this presentation, viewers will learn about: - Study Design - Study Management - Case Studies

1. Antimicrobial Efficacy Studies
Merieux NutriSciences, North America
2016

2.  Clients looking to insure food safety and increase shelf life use
antimicrobial efficacy studies to quantify the effect of an antimicrobial
on their finished products
 These studies measure the effectiveness of antimicrobial agents as
ingredients or processing aids in foods
 Study design is based around a pathogen challenge study and a
holistic shelf life study
 Results detail the suppression of pathogens of interest and show the
effect of the antimicrobial on the shelf life of the finished product
Antimicrobial Efficacy Studies
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3.  In-lab validations
 Samples of finished products are submitted to the lab and inoculated
with pathogens of interest. Products are stored at recommended
storage conditions and pathogens are enumerated over time.
 Un-inoculated samples are also stored for a shelf life study. In this
study, indicator organisms are enumerated over time and sensory and
quality attributes of the products are recorded. Microbial counts and
diminished quality are indicators for the termination of the study.
 In-plant validations
 Samples are inoculated with surrogates that represent the pathogen of
interest but do not represent the food safety risk. These samples are
processed by the client in plant and stored at recommended storage
conditions and pathogens are enumerated over time.
 A shelf life study is also completed on un-inoculated samples as above.
 NOTE: some in-plant processes can be simulated in the lab. Lab
studies allow pathogens to be used, while in-plant studies require
surrogates
Study Design
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4.  Mérieux NutriSciences fully manages antimicrobial efficacy
studies
 Client Consultation
 Experimental Design
 Advising on Cultures/Storage Conditions
 Data Management
 Complete written report, not just COAs
 Full staff for in-plant validations
 ISO 17025 Accredited Research Facilities
 Food Science Center in Crete, IL
 Mérieux NutriSciences, Gainesville, FL
 World renowned research staff and project managers
 Rapid scheduling of projects
 Real-time updates
Study Management
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5.  Fully Cooked Chicken Fillets were provided by the client
 Challenge Study with Listeria cocktail
 Product inoculated with 5 strains and repackaged for storage
 Listeria counts were monitored over 23 days of storage
 Suppression of Listeria was quantified over time
 Shelf Life Study @ 41F
 Aerobic Plate Count
 Lactic Acid Bacteria
 Indicator organism counts and sensory attributes were monitored for a
loss of quality over 23 days
Case Studies—RTE Chicken Fillets
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6.  Turkey Smoked Sausages were manufactured and provided by the
client
 Side-by-side Challenge Study with Listeria cocktail on both Control
and Antimicrobial Treated Samples
 Product inoculated with 5 strains and repackaged for storage
 Listeria counts were monitored over 16 weeks of storage
 Growth of listeria was quantified on control product, while suppression
of Listeria was quantified on treated product
 Shelf Life Study @ 40F
 Aerobic Plate Count
 Lactic Acid Bacteria
 Yeast and Molds
 Indicator organism counts and sensory attributes were monitored for a
loss of quality over 16 weeks
 Extension of shelf life was proven on treated product vs. the control
Case Studies—Turkey Smoked Sausage
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7.  RTE, Sliced turkey deli meat made with antimicrobial was provided
by the client
 Challenge Study with Listeria cocktail
 Product inoculated with 5 strains and repackaged for storage
 Listeria counts were monitored over 70 days of storage
 Suppression of Listeria was quantified over time
 Shelf Life Study @ 4.5C on uninoculated product
 Aerobic Plate Count
 Lactic Acid Bacteria
 Indicator organism counts and sensory attributes were monitored for a
loss of quality over 70 days
Case Studies—Sliced Turkey Deli Meat
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8.  Breadsticks treated with an antimicrobial spray was supplied by the
client
 Challenge Study with L. mono and S. aureus
 Product inoculated and repackaged for storage
 Specific pathogen counts were monitored over 15 days at 45F
 Suppression of pathogens was quantified over time
 Shelf Life Study @ 45F on uninoculated product
 Aerobic Plate Count
 Lactic Acid Bacteria
 Indicator organism counts and sensory attributes were monitored for a
loss of quality over 15 days
Case Studies—RTE Breadsticks
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9. Appendix
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10. Mérieux NutriSciences
Corporate Presentation
March 2016

11. We are dedicated to protecting consumers’ health throughout the world, by
delivering a wide range of testing and consulting services to the food & nutrition
industries, and to water & environment, agro-chemistry, consumer goods,
pharmaceuticals and cosmetics companies.
Our Mission
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12.  Our expertise in food safety and quality has been extended
to six strategic industries with an impact on consumers’ health.
 We provide laboratory, auditing, consultancy, contract research and
education services.
 We offer research services for innovation through the Biofortis
business unit.
Addressing our customers’ challenges
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FOOD SAFETY &
QUALITY
BIOFORTIS
INNOVATIO
N SERVICES
CONSUMER
GOODS
PERSONAL
CARE &
COSMETICS
AGROCHEMICALS WATER &
ENVIRONMENT
PHARMA
& MEDICAL
DEVICES

13. The power of a global network
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Over 80accredited laboratories in 21countries
Europe
40%
Geographical distribution of revenues:
Middle East,
Africa, Asia
Pacific
8%
North America
39%
South America
13%

14. A complete panel of services
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15.  Providing companies around the world with analysis and consultancy
services,
from product design to manufacture and distribution.
Answering the needs of our customers
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A trusted partner:
with scientific expertise
& leadership
Global presence
> 75 accredited laboratories
providing the same level of quality
throughout the world
Customized services
to meet every customer’s needs
PEACE OF MIND GLOBALIZED
SERVICES
STANDARDIZED
QUALITY
TAILOR-MADE
SERVICES
 Strong innovative capabilities and scientific expertise to support customers
in their product development.

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