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Antibody Library Screening
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com
Antibody display and selection platforms
Phage display
Protein-mRNA link via:
-ribosome display
-mRNA display
Display on:
-Yeast
-Bacteria
-Mammalian cells
-Retroviruses
-.....
Microbead via in vitro
compartmentalization
Protein-DNA display
Growth selection via:
-Yeast 2-hybrid
-Protein fragment
complementation
Comparing the main selection platforms for antibodies
Methods for in vitro selection for binding
(a) Immobilized Ag (b) Biotinylated Ag (c) Proximity to other Ag
(d) Recombinant Ag
(e) Eukaryotic/prokaryotic
cells displaying Ag (f) Subcellular fractions
(g) Subtractive cell sorting (h) Tissues with Ag (i) In vivo selection
Steps in antibody selection and screening
B cells
Synthetic
DNA
Antibody
gene pool
Selected antibody lead
Displayed library
B cells
Cloning
of genetic
diversity
Coupling
of geno to
phenotype
Selective
pressure on
phenotype
Screening
Amplification
Selection cycleMutagenesis
and selection
cycle
Creative Biolabs’ Phage Antibody Library Construction Services
Creative Biolabs’ Premade Antibody Libraries
Premade Phage Display
Antibody Libraries
Premade Human Antibody
Library for Yeast Display
Premade Single Domain
Antibody Libraries
Immunized Human
Antibody Libraries
Creative Biolabs’ Antibody Library Screening Services
Solid-phase Library
Screening
Solution-sorting Library
Screening
Cell-based Library
Screening
In vivo Phage Display
Screening
Ex vivo Phage Display
Screening
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com
Antibody Library Screening

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Antibody Library Screening - Creative Biolabs

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  • 9. Address: 45-1 Ramsey Road, Shirley, NY 11967, USA Tel: 1-631-381-2994 Fax: 1-631-207-8356 Email: info@creative-biolabs.com Web: www.creative-biolabs.com Antibody Library Screening