Oar researchhas provedthat lactic acid bacteria possess the wide spectrum of antagonistic activity. They inhibit the
growth of not only agents of intestinal infections but also agents ofdiseases not related to gastrointestinal tract.
This document discusses a study that investigated the antifungal and anti-mycelium activities of biogenic silver, copper, zinc oxide, and gold nanoparticles. The nanoparticles were tested against four fungal strains (Candida albicans, Cryptococcus neoformans, Aspergillus niger, Fusarium oxysporum). The minimum inhibitory concentrations of silver, copper, and zinc oxide nanoparticles were determined to be ≤8 μg/ml for the non-spore forming fungi and ≤16 μg/ml for the spore forming fungi. Anti-mycelium effects were observed for A. niger and F. oxysporum, with silver nanoparticles showing the highest effect at 72.8%. The
The document summarizes an annual probiotic symposium held in Chennai, India in December 2016. It includes an agenda with presentations on the role of probiotics in gastrointestinal diseases, the equilibrium of the gut flora, the history and definitions of probiotics and prebiotics, how probiotics function and their anti-inflammatory and immunomodulatory effects. It also discusses the clinical applications of probiotics in gastrointestinal disorders like diarrhea, IBD, IBS, obesity, and intestinal pain. Studies presented show probiotics can reduce NAFLD in obese children and infections in critical illness.
Mitochondrial Complex 1is Important for Plant Tolerance to Fungal Biotic StressSryahwa Publications
Environmental constraints, such as biotic stress, are detrimental for plant productivity, survival and reproduction. Although plants have evolved metabolic mechanisms to tolerate environmental challenges, our knowledge on the importance of mitochondrial metabolism in biotic stress responses is still fragmentary. This study examined the effects of mutations in mitochondrial complex I (CI) and determined major stress-responsive metabolites associated with decreased tolerance to fungal infection.
Application of probiotics in complex treatment of tuberculosisIJERA Editor
The probiotic bacteria possessing ability to suppress growth of Mycobacterium B5 are revealed. Antagonistic
activity in selected strains studied during the growth on various nutrient media. Strains adapted to the low pH
exposure. They are steady against a number of the antibiotics, used at tuberculosis treatment. This testifies to the
prospects of further studies on the use of probiotics in the
The inhibitory activity of L. crispatus against uropathogenes in vitroIJMCERJournal
ABSTRACT: Bacterial interference refers to the use of bacteria of virulence to compete with and protect against
colonization and infection by disease causing organisms. In this study, L. crispatus strain was used to detect its
antibacterial activity towered five species (one Gram-positive and four Gram-negative ) of the most common bacteria
causing urinary tract infection. L. crispatus completely inhibited growth of Staphylococcus aureus , while it had no
inhibitory effect on three of the other species.
Key Words: inhibitory activity, L. crispatus, uropathogens
Trends in Antibiotic Resistance of Vibrio Cholerae Isolates in Kenya (2006 - ...paperpublications3
Abstract: The evolution of antibiotic resistance was studied among revived Vibrio cholerae strains which were previously archived at -800c between 2006 and 2015. Antibiotic susceptibility testing (AST) on 12 antimicrobials; ampicillin (10µg), cefpodoxime (10 µg), ceftazidime (30 µg), cefotaxime (30 µg), amoxicillin- clavulanic acid (10/ 100 µg ratio) nalidixic acid (30 µg), tetracycline (30 µg), ciprofloxacin (10 µg), SXT (sulphamethoxazole -30 µg trimethoprim -5.2 µg), streptomycin (25 µg), gentamycin (10 µg) and chloramphenicol (30 µg) was carried out using Kirby-Bauer disc diffusion method. AST results revealed susceptibility to tetracycline, which is the drug of choice in Kenya administered as doxycycline during cholera outbreaks, among all isolates. Resistance to βeta-lactams and ciprofloxacin emerged in latter years while a decline in resistance to SXT, Chloramphenicol and Streptomycin was noted. This study gave a clear indication that there were changes in the resistance patterns whereby resistance to some antimicrobials declined and others emerged over the ten year period. In order to slow down the emergence and spread of resistance strains, care should be taken by health professionals when prescribing antimicrobials to patients suffering from cholera disease and should be restricted to only severe cases. It is also recommended that antimicrobial susceptibility testing should be done before giving antimicrobials in management of cholera cases.
Keywords: Antimicrobial resistance, Evolution, Kenya, Vibrio cholera.
Title: Trends in Antibiotic Resistance of Vibrio Cholerae Isolates in Kenya (2006 - 2015)
Author: Penina Muthoni Kung’u, Samuel Njoroge, John Kiiru, Paul Okemo, Samuel Kariuki
ISSN 2349-7823
International Journal of Recent Research in Life Sciences (IJRRLS)
Paper Publications
Quercetin was found to inhibit quorum sensing in foodborne bacteria both in vitro and in silico. In laboratory experiments, quercetin reduced quorum sensing-dependent phenotypes like biofilm formation, exopolysaccharide production, and motility in a concentration-dependent manner in pathogens like Pseudomonas aeruginosa, Yersinia enterocolitica, and Klebsiella pneumoniae. Molecular docking analysis revealed that quercetin binds strongly to the LasR receptor protein involved in quorum sensing. Molecular dynamics simulations further suggested that quercetin inhibits quorum sensing by inducing conformational changes in the receptor-quercetin complex. The study provides evidence that quercetin can act as a competitive inhibitor of qu
This document summarizes research on how maggots combat infection in wounds. It discusses how maggots likely kill bacteria through multiple mechanisms, including ingesting bacteria in their digestive tract and secreting potent antibacterial factors. Recent research has found two discrete antibacterial factors secreted by maggots, including a potent factor under 500 Da that is effective against MRSA. This research improves understanding of maggot secretions' antibacterial properties and their clinical potential for treating antibiotic-resistant infections.
This document discusses a study that investigated the antifungal and anti-mycelium activities of biogenic silver, copper, zinc oxide, and gold nanoparticles. The nanoparticles were tested against four fungal strains (Candida albicans, Cryptococcus neoformans, Aspergillus niger, Fusarium oxysporum). The minimum inhibitory concentrations of silver, copper, and zinc oxide nanoparticles were determined to be ≤8 μg/ml for the non-spore forming fungi and ≤16 μg/ml for the spore forming fungi. Anti-mycelium effects were observed for A. niger and F. oxysporum, with silver nanoparticles showing the highest effect at 72.8%. The
The document summarizes an annual probiotic symposium held in Chennai, India in December 2016. It includes an agenda with presentations on the role of probiotics in gastrointestinal diseases, the equilibrium of the gut flora, the history and definitions of probiotics and prebiotics, how probiotics function and their anti-inflammatory and immunomodulatory effects. It also discusses the clinical applications of probiotics in gastrointestinal disorders like diarrhea, IBD, IBS, obesity, and intestinal pain. Studies presented show probiotics can reduce NAFLD in obese children and infections in critical illness.
Mitochondrial Complex 1is Important for Plant Tolerance to Fungal Biotic StressSryahwa Publications
Environmental constraints, such as biotic stress, are detrimental for plant productivity, survival and reproduction. Although plants have evolved metabolic mechanisms to tolerate environmental challenges, our knowledge on the importance of mitochondrial metabolism in biotic stress responses is still fragmentary. This study examined the effects of mutations in mitochondrial complex I (CI) and determined major stress-responsive metabolites associated with decreased tolerance to fungal infection.
Application of probiotics in complex treatment of tuberculosisIJERA Editor
The probiotic bacteria possessing ability to suppress growth of Mycobacterium B5 are revealed. Antagonistic
activity in selected strains studied during the growth on various nutrient media. Strains adapted to the low pH
exposure. They are steady against a number of the antibiotics, used at tuberculosis treatment. This testifies to the
prospects of further studies on the use of probiotics in the
The inhibitory activity of L. crispatus against uropathogenes in vitroIJMCERJournal
ABSTRACT: Bacterial interference refers to the use of bacteria of virulence to compete with and protect against
colonization and infection by disease causing organisms. In this study, L. crispatus strain was used to detect its
antibacterial activity towered five species (one Gram-positive and four Gram-negative ) of the most common bacteria
causing urinary tract infection. L. crispatus completely inhibited growth of Staphylococcus aureus , while it had no
inhibitory effect on three of the other species.
Key Words: inhibitory activity, L. crispatus, uropathogens
Trends in Antibiotic Resistance of Vibrio Cholerae Isolates in Kenya (2006 - ...paperpublications3
Abstract: The evolution of antibiotic resistance was studied among revived Vibrio cholerae strains which were previously archived at -800c between 2006 and 2015. Antibiotic susceptibility testing (AST) on 12 antimicrobials; ampicillin (10µg), cefpodoxime (10 µg), ceftazidime (30 µg), cefotaxime (30 µg), amoxicillin- clavulanic acid (10/ 100 µg ratio) nalidixic acid (30 µg), tetracycline (30 µg), ciprofloxacin (10 µg), SXT (sulphamethoxazole -30 µg trimethoprim -5.2 µg), streptomycin (25 µg), gentamycin (10 µg) and chloramphenicol (30 µg) was carried out using Kirby-Bauer disc diffusion method. AST results revealed susceptibility to tetracycline, which is the drug of choice in Kenya administered as doxycycline during cholera outbreaks, among all isolates. Resistance to βeta-lactams and ciprofloxacin emerged in latter years while a decline in resistance to SXT, Chloramphenicol and Streptomycin was noted. This study gave a clear indication that there were changes in the resistance patterns whereby resistance to some antimicrobials declined and others emerged over the ten year period. In order to slow down the emergence and spread of resistance strains, care should be taken by health professionals when prescribing antimicrobials to patients suffering from cholera disease and should be restricted to only severe cases. It is also recommended that antimicrobial susceptibility testing should be done before giving antimicrobials in management of cholera cases.
Keywords: Antimicrobial resistance, Evolution, Kenya, Vibrio cholera.
Title: Trends in Antibiotic Resistance of Vibrio Cholerae Isolates in Kenya (2006 - 2015)
Author: Penina Muthoni Kung’u, Samuel Njoroge, John Kiiru, Paul Okemo, Samuel Kariuki
ISSN 2349-7823
International Journal of Recent Research in Life Sciences (IJRRLS)
Paper Publications
Quercetin was found to inhibit quorum sensing in foodborne bacteria both in vitro and in silico. In laboratory experiments, quercetin reduced quorum sensing-dependent phenotypes like biofilm formation, exopolysaccharide production, and motility in a concentration-dependent manner in pathogens like Pseudomonas aeruginosa, Yersinia enterocolitica, and Klebsiella pneumoniae. Molecular docking analysis revealed that quercetin binds strongly to the LasR receptor protein involved in quorum sensing. Molecular dynamics simulations further suggested that quercetin inhibits quorum sensing by inducing conformational changes in the receptor-quercetin complex. The study provides evidence that quercetin can act as a competitive inhibitor of qu
This document summarizes research on how maggots combat infection in wounds. It discusses how maggots likely kill bacteria through multiple mechanisms, including ingesting bacteria in their digestive tract and secreting potent antibacterial factors. Recent research has found two discrete antibacterial factors secreted by maggots, including a potent factor under 500 Da that is effective against MRSA. This research improves understanding of maggot secretions' antibacterial properties and their clinical potential for treating antibiotic-resistant infections.
Isolation and Characterization of Bacteria from Tropical SoilsNicole Rivera
This document describes a study that aimed to isolate bacteria from tropical soils in Puerto Rico that have the potential to produce antibiotics. Soil samples were collected and bacteria were isolated through serial dilution and culturing. Four isolated bacteria were purified and characterized through gram staining, testing for antibiotic production against E. coli and M. luteus, PCR amplification with 16S rRNA primers, and DNA sequencing. Two of the bacteria showed potential as antibiotic producers against M. luteus. The isolation and characterization of soil bacteria with antibiotic properties could help address the growing problem of antibiotic resistance.
This document discusses antibiotic resistance and a new antibiotic called teixobactin. It summarizes that teixobactin is produced by Eleftheria terrae bacteria and has a novel mechanism of action that may be able to evade antibiotic resistance. The document outlines the objectives and methods used to study teixobactin, including cultivating the producing strain, determining its genetic sequence, describing its antimicrobial mechanism and testing its efficacy against resistant bacteria. It concludes that new technologies like iChip will enable discovery of new microbes and antibiotics, teixobactin promises to save lives by being effective against resistant bacteria, and collaborations are needed to address the threat of antibiotic resistance.
Ichip is a novel method for in situ cultivation of environmental microorganisms. A study found that ichips incubated directly in soil and seawater samples led to significantly higher bacterial colony counts compared to traditional petri dishes. Microbial species grown in ichips were also more novel and diverse than those in petri dishes, with little overlap between the two methods. The ichip approach recovered many bacterial strains and genera not previously identified, supporting its ability to cultivate the "uncultivable" majority of environmental microbes.
1) Maggot therapy employs sterile fly larvae to promote wound healing through debridement, disinfection, and stimulation of wound healing.
2) Maggots secrete antibacterial factors that combat wound infections, including potential agents active against methicillin-resistant Staphylococcus aureus (MRSA).
3) Maggots may enhance wound healing through physical stimulation of tissue, secretion of allantoin and ammonia to increase pH, and stimulation of growth factors involved in proliferation and remodeling of extracellular matrix.
— The microbiological content of Lettuce (a vegetable), commonly vended in the Benin metropolis of Edo state were evaluated. Five vending locations were chosen for the study. Whole and soft rot samples were purchased and analysed for microbiological composition. Results showed high counts in soft rot samples in lettuce. Nutrient agar plated lettuce samples had bacterial counts in the range of 2.0x 103 to 4.7x10 7. Pseudomonas species was the dominant species found in lettuce samples. Bacillus species was isolated from one location in the lettuce samples. Mac Conkey agar plated lettuce plated had bacterial counts in the range of 2.3 x 10 3 to 5.7x 10 7. Enterobacter species, E. coli, and Klebsiella species were the dominant species isolated. Though, Proteus species was isolated from lettuce samples obtained from location five only. The study observes that consuming soft rot samples could pose a risk of introducing pathogens to the consumer due to their high microbial counts and could be detrimental to the health of the consumer.
Preventive role of probiotics to face SARS-CoV-2 pandemicHussein Al-tameemi
This document discusses probiotics and their potential preventive role against SARS-CoV-2. It defines probiotics as live microorganisms that provide health benefits when consumed. Common probiotic genera include Lactobacillus, Bifidobacterium, and Enterococcus. Probiotics may have antiviral effects through producing inhibitory substances, direct interaction with viruses, and stimulating the immune system. Studies have found probiotics can reduce severity of respiratory, GI, and other viral infections. The document recommends consuming probiotic foods during the SARS-CoV-2 pandemic and establishing guidelines for their use.
1) The study evaluated the antimicrobial activity of crude culture filtrate and methanol extract of the mushroom Stereum ostrea against both Gram-positive and Gram-negative bacteria.
2) Crude culture filtrate showed higher inhibitory activity compared to methanol extract, as evidenced by larger inhibition zones. The crude filtrate was most effective against Bacillus subtilis and least effective against Klebsiella pneumoniae.
3) The minimal inhibitory concentration of both crude and methanol extracts was 20 μl for Bacillus subtilis and 30 μl for the other bacteria tested, indicating Stereum ostrea was most potent against Bacillus subtilis.
Identification and characterization of actinomycetes forAlexander Decker
This document describes a study that identified and characterized two actinomycete bacterial strains with
antagonistic activity against Streptomyces scabies, the pathogen that causes potato scab disease. The two strains
were isolated from soil samples. Molecular characterization using 16S rDNA gene sequencing identified the first
strain as Streptomyces avermitilis and the second strain as Actinomyces odontolyticus. Both strains showed high
levels of antibiosis against S. scabies in in vitro and in vivo assays, effectively controlling potato scab disease
symptoms on potato varieties Cara and Diamond.
Isolation and Identification of Fungi from fast food restaurants in Langa BazarIJEAB
A total of (218) samples from Eleven different foods were processed between October 2016 and February 2017 which include (Tomato, Chicken meat, red meat, falafel, potato, bread, eggplant, cabbage, celery, cucumber and onion). Samples were collected from 4 different fast food restaurants inoculated on Potato dextrose agar and Sabouraud Dextrose Agar. Isolated fungus identified morphologically and microscopically in accordance with standard procedures. Results showed that six fungal genera were associated with the selected fast food restaurants. The isolated fungal genera were Aspergillus sp., Alternaria sp., Mucor sp., Rhizopus sp., Saccharomyces sp., Brettanomyces sp. The number of total colonies in October were 236 and in February were 119 and the number of colonies were higher when cultured on Potato dextrose agar than Sabouraud Dextrose Agar. There was variation in the pattern of occurrence of the fungus in fast foods Aspergillus sp. appears to be the most pathogenic fungi that present in the food samples.
This study investigated the effectiveness of pulsed UV (PUV) light irradiation in damaging Bacillus cereus and Bacillus megaterium, two bacteria relevant to food safety issues. PUV treatment was found to inflict four types of cellular damage that prevented DNA replication. Total reduction of both bacteria was seen at 120P and 160P of PUV treatment. Bacillus megaterium was more sensitive, experiencing total reduction at 80P as well. Increased concentrations of leaked intracellular proteins also demonstrated the damaging effects of PUV. However, protein leakage did not correlate to reductions in viable bacterial counts. Further research is needed to determine the relationship between protein damage and DNA damage from PUV and its effects on pathogenic toxins produced by these bacteria
This document summarizes a study that isolated three strains of Lactobacillus (L. plantarum, L. acidophilus, L. casei) from human intestinal samples. It evaluated the antibacterial activity of these strains against five strains of Staphylococcus aureus, including three methicillin-resistant S. aureus strains. L. casei showed the highest inhibitory activity. The study aimed to explore the potential of isolated intestinal Lactobacillus strains to control methicillin-resistant S. aureus infections.
This document discusses bacteriophages and their applications in food. It begins with an introduction to bacteriophages, their classification, life cycle and genomic structure. It then discusses how bacteriophages can be used as biocontrol agents for foodborne pathogens in various industries and products. It also reviews some studies on bacteriophages for controlling foodborne pathogens. Large-scale production and purification strategies are described. Some companies involved in bacteriophage products are listed, along with advantages and challenges of using bacteriophages. Applications of bacteriophages in detection of pathogens are also summarized.
This document provides a review of antimicrobial resistance in fish aquaculture. It discusses the increasing use of antimicrobial agents in aquaculture and the development and spread of resistance. Key points include:
1) Antimicrobial agents are commonly used in aquaculture for disease treatment and growth promotion, but their overuse and misuse has led to the development of resistance.
2) Resistance genes can be transferred between bacteria through horizontal gene transfer, affecting the whole ecosystem.
3) Wastewater from aquaculture facilities contains high levels of antimicrobial residues and resistant bacteria, acting as reservoirs for resistance development.
4) There are risks to both animal and human health from antimicrobial resistant
This document summarizes a study that evaluated the antimicrobial activity of crude culture filtrate and methanol extract of the mushroom Stereum ostrea against bacteria. Key findings:
- Crude culture filtrate of S. ostrea showed the highest inhibitory activity against both gram-positive and gram-negative bacteria based on zone of inhibition tests, with the strongest effect against Bacillus subtilis. Methanol extract also inhibited bacterial growth but to a lesser degree.
- The minimum inhibitory concentration of both crude extract and methanol extract was 20μl for B. subtilis and 30μl for other tested bacteria.
- Results indicate S. ostrea contains metabolites with potential broad-spectrum antimicrobial properties that
This document summarizes a study examining the incidence and antibiotic sensitivity of Bacillus cereus isolated from ready-to-eat foods sold in markets in Port Harcourt, Nigeria. Sixty-four food samples were tested and Bacillus cereus was found in all samples, with the highest frequency occurring in cooked rice (29.51%) and masa (26.23%). All Bacillus cereus isolates were susceptible to several antibiotics including rifampin, chloramphenicol, and erythromycin, but were 100% resistant to norfloxapin, floxapen, and ampiclox. The presence of Bacillus cereus in foods sold in markets poses a public health risk due to poor sanitation
This study analyzed the antibiotic susceptibility patterns of gram-negative bacteria isolated from clinical samples at a large specialty hospital in India from 2014. It found that 81% of bacterial isolates were gram-negative bacilli, led by Enterobacteriaceae species, Acinetobacter spp., and Pseudomonas aeruginosa. Antibiotic resistance rates were high and increasing among these pathogens, particularly to beta-lactams, cephalosporins, fluoroquinolones, and carbapenems. The results demonstrate the growing threat of multidrug-resistant gram-negative infections and emphasize the need for prudent antibiotic use.
This document proposes research on experimenting with a novel seaweed extract to control Pseudomonas infections. Pseudomonas aeruginosa commonly causes infections and is difficult to treat due to antibiotic resistance. The aim is to screen Pseudomonas strains using a micro-assay to test if the seaweed extract inhibits their growth. Seaweed extracts contain bioactive compounds and have potential pharmaceutical benefits. The proposed methodology involves culturing bacteria, analyzing growth with and without the extract, and examining changes at the DNA and carbohydrate levels to evaluate the extract's effects on bacterial exploitation and possible adaptation. The goal is to develop a cheap, effective, and environmentally friendly treatment for Pseudomonas infections.
This document describes a study that aimed to isolate and characterize antibiotic-degrading bacteria from soil on a pig farm for penicillin V potassium (PVK). Four bacterial isolates (LM-1, LM-2, LM-3, LM-4) were obtained from the soil. LM-1 and LM-2 showed the highest degradation rates of PVK and were identified as belonging to the Bacillus genus. LM-1 and LM-2 were also found to be resistant to PVK in antibiotic sensitivity tests. Quantitative tests showed LM-1 and LM-2 had degradation rates of 68% and 66% of PVK, respectively, when the initial PVK concentration was 100 μg/mL. The study suggests LM-
Enkelvoudige registratie aan de bron, meervoudig gebruik: FeIT of fICTie?Furore_com
Het idee dat ICT in zorg de registratielast verlaagt, is nog niet terug te zien in de praktijk. Wietske Huizenga en Lililan Minne vertellen in deze presentatie aan de hand van twee praktijkvoorbeelden hoe de registratielast omlaag kan, door geode inrichting van de registratie aan de bron.
Isolation and Characterization of Bacteria from Tropical SoilsNicole Rivera
This document describes a study that aimed to isolate bacteria from tropical soils in Puerto Rico that have the potential to produce antibiotics. Soil samples were collected and bacteria were isolated through serial dilution and culturing. Four isolated bacteria were purified and characterized through gram staining, testing for antibiotic production against E. coli and M. luteus, PCR amplification with 16S rRNA primers, and DNA sequencing. Two of the bacteria showed potential as antibiotic producers against M. luteus. The isolation and characterization of soil bacteria with antibiotic properties could help address the growing problem of antibiotic resistance.
This document discusses antibiotic resistance and a new antibiotic called teixobactin. It summarizes that teixobactin is produced by Eleftheria terrae bacteria and has a novel mechanism of action that may be able to evade antibiotic resistance. The document outlines the objectives and methods used to study teixobactin, including cultivating the producing strain, determining its genetic sequence, describing its antimicrobial mechanism and testing its efficacy against resistant bacteria. It concludes that new technologies like iChip will enable discovery of new microbes and antibiotics, teixobactin promises to save lives by being effective against resistant bacteria, and collaborations are needed to address the threat of antibiotic resistance.
Ichip is a novel method for in situ cultivation of environmental microorganisms. A study found that ichips incubated directly in soil and seawater samples led to significantly higher bacterial colony counts compared to traditional petri dishes. Microbial species grown in ichips were also more novel and diverse than those in petri dishes, with little overlap between the two methods. The ichip approach recovered many bacterial strains and genera not previously identified, supporting its ability to cultivate the "uncultivable" majority of environmental microbes.
1) Maggot therapy employs sterile fly larvae to promote wound healing through debridement, disinfection, and stimulation of wound healing.
2) Maggots secrete antibacterial factors that combat wound infections, including potential agents active against methicillin-resistant Staphylococcus aureus (MRSA).
3) Maggots may enhance wound healing through physical stimulation of tissue, secretion of allantoin and ammonia to increase pH, and stimulation of growth factors involved in proliferation and remodeling of extracellular matrix.
— The microbiological content of Lettuce (a vegetable), commonly vended in the Benin metropolis of Edo state were evaluated. Five vending locations were chosen for the study. Whole and soft rot samples were purchased and analysed for microbiological composition. Results showed high counts in soft rot samples in lettuce. Nutrient agar plated lettuce samples had bacterial counts in the range of 2.0x 103 to 4.7x10 7. Pseudomonas species was the dominant species found in lettuce samples. Bacillus species was isolated from one location in the lettuce samples. Mac Conkey agar plated lettuce plated had bacterial counts in the range of 2.3 x 10 3 to 5.7x 10 7. Enterobacter species, E. coli, and Klebsiella species were the dominant species isolated. Though, Proteus species was isolated from lettuce samples obtained from location five only. The study observes that consuming soft rot samples could pose a risk of introducing pathogens to the consumer due to their high microbial counts and could be detrimental to the health of the consumer.
Preventive role of probiotics to face SARS-CoV-2 pandemicHussein Al-tameemi
This document discusses probiotics and their potential preventive role against SARS-CoV-2. It defines probiotics as live microorganisms that provide health benefits when consumed. Common probiotic genera include Lactobacillus, Bifidobacterium, and Enterococcus. Probiotics may have antiviral effects through producing inhibitory substances, direct interaction with viruses, and stimulating the immune system. Studies have found probiotics can reduce severity of respiratory, GI, and other viral infections. The document recommends consuming probiotic foods during the SARS-CoV-2 pandemic and establishing guidelines for their use.
1) The study evaluated the antimicrobial activity of crude culture filtrate and methanol extract of the mushroom Stereum ostrea against both Gram-positive and Gram-negative bacteria.
2) Crude culture filtrate showed higher inhibitory activity compared to methanol extract, as evidenced by larger inhibition zones. The crude filtrate was most effective against Bacillus subtilis and least effective against Klebsiella pneumoniae.
3) The minimal inhibitory concentration of both crude and methanol extracts was 20 μl for Bacillus subtilis and 30 μl for the other bacteria tested, indicating Stereum ostrea was most potent against Bacillus subtilis.
Identification and characterization of actinomycetes forAlexander Decker
This document describes a study that identified and characterized two actinomycete bacterial strains with
antagonistic activity against Streptomyces scabies, the pathogen that causes potato scab disease. The two strains
were isolated from soil samples. Molecular characterization using 16S rDNA gene sequencing identified the first
strain as Streptomyces avermitilis and the second strain as Actinomyces odontolyticus. Both strains showed high
levels of antibiosis against S. scabies in in vitro and in vivo assays, effectively controlling potato scab disease
symptoms on potato varieties Cara and Diamond.
Isolation and Identification of Fungi from fast food restaurants in Langa BazarIJEAB
A total of (218) samples from Eleven different foods were processed between October 2016 and February 2017 which include (Tomato, Chicken meat, red meat, falafel, potato, bread, eggplant, cabbage, celery, cucumber and onion). Samples were collected from 4 different fast food restaurants inoculated on Potato dextrose agar and Sabouraud Dextrose Agar. Isolated fungus identified morphologically and microscopically in accordance with standard procedures. Results showed that six fungal genera were associated with the selected fast food restaurants. The isolated fungal genera were Aspergillus sp., Alternaria sp., Mucor sp., Rhizopus sp., Saccharomyces sp., Brettanomyces sp. The number of total colonies in October were 236 and in February were 119 and the number of colonies were higher when cultured on Potato dextrose agar than Sabouraud Dextrose Agar. There was variation in the pattern of occurrence of the fungus in fast foods Aspergillus sp. appears to be the most pathogenic fungi that present in the food samples.
This study investigated the effectiveness of pulsed UV (PUV) light irradiation in damaging Bacillus cereus and Bacillus megaterium, two bacteria relevant to food safety issues. PUV treatment was found to inflict four types of cellular damage that prevented DNA replication. Total reduction of both bacteria was seen at 120P and 160P of PUV treatment. Bacillus megaterium was more sensitive, experiencing total reduction at 80P as well. Increased concentrations of leaked intracellular proteins also demonstrated the damaging effects of PUV. However, protein leakage did not correlate to reductions in viable bacterial counts. Further research is needed to determine the relationship between protein damage and DNA damage from PUV and its effects on pathogenic toxins produced by these bacteria
This document summarizes a study that isolated three strains of Lactobacillus (L. plantarum, L. acidophilus, L. casei) from human intestinal samples. It evaluated the antibacterial activity of these strains against five strains of Staphylococcus aureus, including three methicillin-resistant S. aureus strains. L. casei showed the highest inhibitory activity. The study aimed to explore the potential of isolated intestinal Lactobacillus strains to control methicillin-resistant S. aureus infections.
This document discusses bacteriophages and their applications in food. It begins with an introduction to bacteriophages, their classification, life cycle and genomic structure. It then discusses how bacteriophages can be used as biocontrol agents for foodborne pathogens in various industries and products. It also reviews some studies on bacteriophages for controlling foodborne pathogens. Large-scale production and purification strategies are described. Some companies involved in bacteriophage products are listed, along with advantages and challenges of using bacteriophages. Applications of bacteriophages in detection of pathogens are also summarized.
This document provides a review of antimicrobial resistance in fish aquaculture. It discusses the increasing use of antimicrobial agents in aquaculture and the development and spread of resistance. Key points include:
1) Antimicrobial agents are commonly used in aquaculture for disease treatment and growth promotion, but their overuse and misuse has led to the development of resistance.
2) Resistance genes can be transferred between bacteria through horizontal gene transfer, affecting the whole ecosystem.
3) Wastewater from aquaculture facilities contains high levels of antimicrobial residues and resistant bacteria, acting as reservoirs for resistance development.
4) There are risks to both animal and human health from antimicrobial resistant
This document summarizes a study that evaluated the antimicrobial activity of crude culture filtrate and methanol extract of the mushroom Stereum ostrea against bacteria. Key findings:
- Crude culture filtrate of S. ostrea showed the highest inhibitory activity against both gram-positive and gram-negative bacteria based on zone of inhibition tests, with the strongest effect against Bacillus subtilis. Methanol extract also inhibited bacterial growth but to a lesser degree.
- The minimum inhibitory concentration of both crude extract and methanol extract was 20μl for B. subtilis and 30μl for other tested bacteria.
- Results indicate S. ostrea contains metabolites with potential broad-spectrum antimicrobial properties that
This document summarizes a study examining the incidence and antibiotic sensitivity of Bacillus cereus isolated from ready-to-eat foods sold in markets in Port Harcourt, Nigeria. Sixty-four food samples were tested and Bacillus cereus was found in all samples, with the highest frequency occurring in cooked rice (29.51%) and masa (26.23%). All Bacillus cereus isolates were susceptible to several antibiotics including rifampin, chloramphenicol, and erythromycin, but were 100% resistant to norfloxapin, floxapen, and ampiclox. The presence of Bacillus cereus in foods sold in markets poses a public health risk due to poor sanitation
This study analyzed the antibiotic susceptibility patterns of gram-negative bacteria isolated from clinical samples at a large specialty hospital in India from 2014. It found that 81% of bacterial isolates were gram-negative bacilli, led by Enterobacteriaceae species, Acinetobacter spp., and Pseudomonas aeruginosa. Antibiotic resistance rates were high and increasing among these pathogens, particularly to beta-lactams, cephalosporins, fluoroquinolones, and carbapenems. The results demonstrate the growing threat of multidrug-resistant gram-negative infections and emphasize the need for prudent antibiotic use.
This document proposes research on experimenting with a novel seaweed extract to control Pseudomonas infections. Pseudomonas aeruginosa commonly causes infections and is difficult to treat due to antibiotic resistance. The aim is to screen Pseudomonas strains using a micro-assay to test if the seaweed extract inhibits their growth. Seaweed extracts contain bioactive compounds and have potential pharmaceutical benefits. The proposed methodology involves culturing bacteria, analyzing growth with and without the extract, and examining changes at the DNA and carbohydrate levels to evaluate the extract's effects on bacterial exploitation and possible adaptation. The goal is to develop a cheap, effective, and environmentally friendly treatment for Pseudomonas infections.
This document describes a study that aimed to isolate and characterize antibiotic-degrading bacteria from soil on a pig farm for penicillin V potassium (PVK). Four bacterial isolates (LM-1, LM-2, LM-3, LM-4) were obtained from the soil. LM-1 and LM-2 showed the highest degradation rates of PVK and were identified as belonging to the Bacillus genus. LM-1 and LM-2 were also found to be resistant to PVK in antibiotic sensitivity tests. Quantitative tests showed LM-1 and LM-2 had degradation rates of 68% and 66% of PVK, respectively, when the initial PVK concentration was 100 μg/mL. The study suggests LM-
Enkelvoudige registratie aan de bron, meervoudig gebruik: FeIT of fICTie?Furore_com
Het idee dat ICT in zorg de registratielast verlaagt, is nog niet terug te zien in de praktijk. Wietske Huizenga en Lililan Minne vertellen in deze presentatie aan de hand van twee praktijkvoorbeelden hoe de registratielast omlaag kan, door geode inrichting van de registratie aan de bron.
Dokumen tersebut memberikan panduan lengkap tentang teknik-teknik pembalutan luka yang meliputi berbagai bagian tubuh seperti kepala, tangan, siku, dada, perut, dan kaki. Teknik-teknik tersebut mencakup cara meletakkan pembalut, membuat lilitan silang, dan mengikat ujung pembalut untuk mengontrol perdarahan dan memberikan kesejahteraan.
The document provides details of a proposed plan to construct a 10,000 seat FIFA standard international soccer stadium in Corozal Town, Belize. The total estimated cost of construction is $12.7 million USD. The stadium aims to provide facilities and opportunities for youth in Belize to develop their soccer talents, reduce unemployment, and benefit the local economy through hosting events. Revenue from the stadium is estimated to be over $1.9 million BZE annually from sources such as luxury suites, ticketing, concessions, parking, and concerts.
This document discusses the importance of security programs for condo communities. It recommends that condo boards implement comprehensive security programs that include physical, technological, and procedural security measures, as well as security personnel. The security program should be integrated and continuously reviewed through audits. The document emphasizes that resident involvement is also key - a safe community involves residents reporting issues and being aware of suspicious activity. The biggest security investment is encouraging residents to invest in their community.
- There are three types of faith that living beings can have based on the modes of material nature - faith in goodness, passion, or ignorance. Acts performed with faith in passion or ignorance only yield temporary material results, while acts performed with faith in goodness and according to scriptural injunctions purify the heart.
- One's faith is determined by the modes of nature they have acquired from their previous activities. While originally transcendental, when one comes in contact with material nature they take on qualities of sattva, rajas, or tamas.
- People with faith in goodness worship demigods, those with faith in passion worship demons, and those with faith in ignorance worship ghosts.
Read the notes here https://goo.gl/u3fif4
I'm sharing my experience with the programming, the entrepreneurship, personal growing and society awareness trough the Kaba Gaida (Bulgarian Bagpipe) perspective.
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Krishna explains to Arjuna that all wonderful phenomena showing power, beauty, grandeur or sublimity in the material or spiritual worlds are partial manifestations of Krishna's divine energies and opulence. As the supreme cause and essence of everything, Krishna is the supreme object of worship. He then lists and explains various qualities of living beings such as intelligence, knowledge, forgiveness and their opposites. Krishna states that he alone creates all these various qualities in different arrangements for living beings.
Anti-Virulence Potential And In Vivo Toxicity Of Persicaria Maculosa And Bist...Jose Katab
1) The study evaluated the anti-virulence potential and safety of ethanol extracts from Persicaria maculosa and Bistorta officinalis against the opportunistic pathogen Pseudomonas aeruginosa.
2) The extracts showed anti-virulence activity by inhibiting biofilm formation by up to 50%, reducing pyocyanin production by up to 59%, and completely halting swarming motility of P. aeruginosa at a subinhibitory concentration of 50 μg/mL.
3) Neither extract was toxic in a zebrafish model, representing an initial step towards integrating P. maculosa and B. officinalis in treating Pseudomonas infections.
Antimicrobial activity of Lactobacillus spp. especially (L. planetarium and L. acidophilus) against S. aureus were tested using agar-plug, agar well diffusion methods to select the best isolate that could inhibit the growth of multidrug resistance isolates. Further identification for the presence of bacteriocin was done using ELISA kit. Results showed that Lactobacillus spp isolates were bacteriocin producers with different degrees and that L. planetarium (L7) was the most efficient in bacteriocin production. Therefore, L. planetarium (L7) was selected for purification using 70% saturated ammonium sulfate and gel chromatography. The effect of purified bacteriocin was tested on 16 bacterial isolates using micro-titer plate method and well diffusion method. The results showed the ability of the bacteriocin to inhibit bacteria only at concentrations 1866U/ml (50%), 3732U/ml (100%) with a diameter of inhibition zones ranges between (11-23 mm) respectively. The anti-biofilm activity of purified bacteriocin at concentration 100% was investigated and the results showed that biofilm formation was reduced by 100% in the presence of bacteriocin.
Limitations in the screening of potentially anti-cryptosporidial agents using...UniversitasGadjahMada
The emergence of cryptosporidiosis, a zoonotic disease of the gastrointestinal and respiratory tract caused by Cryptosporidium Tyzzer, 1907, triggered numerous screening studies of various compounds for potential anti-cryptosporidial activity, the majority of which proved ineffective. Extracts of Indonesian plants, Piper betle and Diospyros sumatrana, were tested for potential anticryptosporidial activity using Mastomys coucha (Smith), experimentally inoculated with Cryptosporidium proliferans Kváč, Havrdová, Hlásková, Daňková, Kanděra, Ježková, Vítovec, Sak, Ortega, Xiao, Modrý, Chelladurai, Prantlová et McEvoy, 2016. None of the plant extracts tested showed significant activity against cryptosporidia; however, the results indicate that the following issues should be addressed in similar experimental studies. The monitoring of oocyst shedding during the entire experimental trial, supplemented with histological examination of affected gastric tissue at the time of treatment termination, revealed that similar studies are generally unreliable if evaluations of drug efficacy are based exclusively on oocyst shedding. Moreover, the reduction of oocyst shedding did not guarantee the eradication of cryptosporidia in treated individuals. For treatment trials performed on experimentally inoculated laboratory rodents, only animals in the advanced phase of cryptosporidiosis should be used for the correct interpretation of pathological alterations observed in affected tissue. All the solvents used (methanol, methanol-tetrahydrofuran and dimethylsulfoxid) were shown to be suitable for these studies, i.e. they did not exhibit negative effects on the subjects. The halofuginone lactate, routinely administered in intestinal cryptosporidiosis in calves, was shown to be ineffective against gastric cryptosporidiosis in mice caused by C. proliferans. In contrast, the control application of extract Arabidopsis thaliana, from which we had expected a neutral effect, turned out to have some positive impact on affected gastric tissue.
Microbiome: The genes and genomes of the microbiota, as well as the products of the microbiota and the host environment” [the collective genomes of the micro-organisms in a particular environment. Although the composition of the gut microbiota varies between individuals, the community in each individual is relatively stable over time.
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Shrimps survive white spot syndrome virus challenge following treatment with...Don Rahul
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2.11_Golam Sarower_Probiotics in aquaculture competitive exclusion of pathoge...WorldFish
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Protein was extracted from muscles of Channa striatus and attempts were
made to evaluate in vitro antibacterial activity against clinical bacterial isolates. The
higher concentration of protein (100μg/ml) extracts exhibited a pronounced activity
against Pseudomonas aeruginosa (21 mm), Proteus vulgaris (19 mm), Citrobacter sp
(19 mm), Klebsiella pneumoniae (18 mm), Micrococcus sp (17 mm), Bacillus subtilis (16
mm), Staphylococcus aureus (15 mm), E. coli (14 mm) and Serratia marcescens (5
mm). The minimum inhibitory concentration and minimum bactericidal concentration
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This document describes a study on the biological control of bacterial onion diseases using the bacterium Pantoea agglomerans strain 2066-7. The study involved screening 77 microorganisms isolated from different plant and environmental sources for antagonistic activity against four bacterial onion pathogens (Pseudomonas marginalis, Pseudomonas viridiflava, Xanthomonas retroflexus, and Pantoea ananatis). Pantoea agglomerans strain 2066-7 showed the strongest antagonistic activity against all four pathogens in vitro. Further experiments found that strain 2066-7 provided complete control of P. marginalis and P. viridiflava at a concentration of 107 CFU/ml,
The study analyzed urine samples from 100 UTI patients and isolated Proteus bacteria, identifying 10 as ESBL producers. The ESBL-producing Proteus isolates were resistant to several antibiotics but sensitive to imipenem, amikacin, ciprofloxacin, and meropenem. Aqueous extracts of four plants were tested against Proteus isolates, with Hibiscus Rosa-sinensis demonstrating the highest antibacterial activity. The prevalence of ESBL-producing Proteus in urine samples was 10%, with most gram-negative bacteria sensitive to amikacin, nitrofurantoin, and gentamicin.
American Journal of Bioterrorism, Biosecurity and Biodefense is an international scholarly peer reviewed Open Access journal, aims to promote the research in all the related fields of Bioterrorism, Biosecurity and Biodefence.
American Journal of Bioterrorism, Biosecurity and Biodefense is a comprehensive Open Access peer reviewed scientific Journal that covers multidisciplinary fields. We provide limitless access towards accessing our literature hub with colossal range of articles. The journal aims to publish high quality varied article types such as Research, Review, Short Communications, Case Reports, Perspectives (Editorials), Clinical Images.
American Journal of Bioterrorism, Biosecurity and Biodefense support the scientific modernization and enrichment in Bioterrorism, Biosecurity and Biodefense research community by magnifying access to peer reviewed scientific literary works. Austin also brings universally peer reviewed member journals under one roof thereby promoting knowledge sharing, collaborative and promotion of multidisciplinary science.
PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF ESBL IN SOKOTO PDFNuhu Tanko
This study examined the prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamase (ESBL)-producing gram-negative uropathogens in Sokoto, Nigeria. 365 urine samples were collected and analyzed between November 2014 and February 2015. Gram-negative uropathogens made up 60.9% of positive cultures. The most common isolates were E. coli (29.7%) and Salmonella arizonae (23.4%). 15 isolates (23.3%) were confirmed as ESBL producers, with E. coli and Enterobacter gergoviae being the most common. The isolates showed high resistance to cotrimoxazole but high susceptibility to nitrofurantoin. This study demonstrates
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Advanced control scheme of doubly fed induction generator for wind turbine us...IJECEIAES
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Understanding Inductive Bias in Machine LearningSUTEJAS
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Antagonism of lactic acid bacteria against pathogenic agents not related to gastrointestinal tract
1. N.Gavrilova et al. Int. Journal of Engineering Research and Applications www.ijera.com
ISSN: 2248-9622, Vol. 5, Issue 10, (Part - 2) October 2015, pp.13-16
www.ijera.com 13 | P a g e
Antagonism of lactic acid bacteria against pathogenic agents not
related to gastrointestinal tract
N.Gavrilova, I. Ratnikova, A. Sadanov, K. Bayakysheva, Z.Tourlybaeva, L.
Kosheleva
(Microbiology and Virology Institute at the Ministry of Education and Science of the Republic of Kazakhstan,
Almaty)
ABSTRACT
Oar researchhas provedthat lactic acid bacteria possess the wide spectrum of antagonistic activity. They inhibit the
growth of not only agents of intestinal infections but also agents ofdiseases not related to gastrointestinal tract.
Keywords- lactic acid bacteria, antagonistic activity in vitro, infections located outside the gastrointestinal tract
I. INTRODUCTION
Considerable quantity of works is devoted to
antagonistic activity of lactic acid bacteria. The
researches mainly concern the struggle with
pathogenic gut organisms of a human being and
animals [1-4].
Meanwhile there are cases of effective treatment
of some pathological processes not related with the
gastrointestinal tract, when using biopreparations
from living germ culture - representatives of
normoflora [5, 6]. So, Yugoslavian scientists have
paid attention to the established acceleration of the
healing process of wounds and abrasions in patients
taking enterally food substances or preparations on the
basis of living germ cultures - representatives of
Lactobacillus or Bacillus. French scientists have
published data about successful treatment of patients
with pyelonephritis with the help of the preparation
used for treatment of gastrointestinal disturbance
[7].Armenian scientists have suggested that it should
be possible to use products of metabolism of lactic
acid bacteria to treat septic wounds [8]. It is reported
that 1,4% of lactic acid bacteria isolated when
fermenting fish food possess antilisterious activity [9].
In connection with the above-stated the researches on
revealing of antagonistic activity against pathogen
agents not connected with gastrointestinal tract are of
great interest.
II. MATERIAL AND METHODS
50 strains of lactic acid bacteria isolated from
healthy people and animals were used in the study:
Lactobacillus plantarum1, 1n, l0d, 53n, 16n, 7k, 25m,
14d, 311, 17d1Lactobacillus fermentum19n, 50, 15, 16,
21, 175-2, 27, 17, 7n; Lactobacillus brevis65n, 2d, 272,
166; Lactobacillus cellobiosus20, 7n, 28, 58n, 9, 26c;
Lactobacillus casei139, 173a, 7, 261,
271;Lactobacillus acidophilus 15; Lactobacillus
curvatus18d; Streptococcus lactis43n, 33n, 6nc, 41n;
Streptococcusfaecium14в; Streptococcus
faecalis.104-1, 48n; Streptococcus salivarius20n, 3d,
172; Streptococcus cremoris21n, lm; Streptococcus
thermophilus3d5, 31.
Strains were cultivated on MRS medium and on
combined nutrient medium [10].
Antagonistic activity was determined on the basis of
diffusion method in agar [11] with respect to growth
suppression zones of the following test-cultures:
Staphylocaccusaureus, Bacillus subtilis, Vibriu
scholerae, Bacillus anthracis,
Bacieriumcarotavarum, Bacillus mycoides,
Pasteurellamultocida, Mycabacierium В5,
Pseudomonas aeruginosa, Brucellamelitensis16m,
Brucellaabortus54, Brucellasuis1330, Brucellaavis
066, Brucellaneatamae, Candida albicans,
Aspergillusniger.
In order to test the antibiotic-sensitiveness of
bacteria standard disks impregnated with appropriate
antibiotics were used.
Tests were repeated not less than three times.
III. RESULTS AND DISCUSSION
The most sensitive to lactic acid bacteria action
were S.aureus, B.subtilis, B.anthracis,
B.carotovorum. At that the quantity of antagonists
among the lactobacteria cultivated on combined
medium was accordingly 90,3; 87,1; 77,4%, and on
MRS medium - 71; 67,7; 87,1; 76,7% accordingly.
The most stable to lactobacteria influence was
B.mycoidesthe growth of which was suppressed by
45% of lactobacteria strains cultivated on combined
medium and 58%) - on MRS medium.
Active antagonists against B.carotovarumwere
strains of L. plantarum1, L.cellobiosus20 and
L.plantarum53n(diameter of the growth suppression
zone is 22mm), L.fermentum15 (diameter - 24mm),
cultivated on combined medium and S. cremoris21n
(28mm) - on MRS medium.
RESEARCH ARTICLE OPEN ACCESS
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The growth of B. mycoideswas actively suppressed by
strains of L. plantarum1 (30mm) and L.brevis65n
(27mm) cultivated on combined medium; against
S.aureus— L.brevis65n (32mm), L. plantarum1,
L.plantaruml0d (28mm accordingly) when cultivating
in combined medium, and S.cremoris21n - on MRS
medium; B.subtilis - strains of L.cellobiosus7n
(18mm), S.thermophilus31 (20mm) and S.lactis6nc
(17mm), cultivated in combined medium.
Antagonistic activity against V. choleraewas
revealed in 74% of lactobacteria strains cultivated in
combined medium, at that none of them showed the
activity when cultivating in MRS medium. The high
activity with respect to the given culture was
registered in L.plantarum1 (18mm), S.faecium14B
(17mm), L.brevis65n, L. fermentum15, S.
cremoris21n and L. plantarum16n (16mm
accordingly), the rest strains gave suppression growth
zones of the test-culture from 9 till 13 mm.
64%o of the tested lactobacteria strains suppress
the growth of P.aeruginosatest-culture,a main
pathogen of hospital infection of Surgical Department
at the Urology Research Center of the Ministry of
Health of Kazakhstan.
We have formed the association from lactic acid
bacteria (L. casei139, L. fermentum127n, L. casei173a,
L. brevisБ-3) and propionic acid bacteria. The
association possesses high antagonistic activity
against C.albicans,S. aureusand P. aeruginosaclinical
strain. The association is stable to antibiotics used for
treatment of inflammatory diseases: cefoperazonome
(30mkg/ml), cefamandole (30mkg/ml), seftacidine
(30 mkg/ml), norbaxine (l0 mkg/ml), ofloxacine
(5mkg/ml), cifrane (30mkg/ml).
Testing of the associations in vivo was carried out
in the Dzharbusynov Research Center of Urology on
patients with benign hyperplasia of prostate of 1-2
degree complicated with the inflammation of inferior
urinary tracts after the catheterization. Before
treatment urine of those patients was infected by such*
pathogens like S.aureus, P.aeruginosa, P. vulgaris in
titer more than 10000 microbiol cells in 1 ml.
Together with complex antibacterial therapy 50-100
ml of liquid culture of the association of lactic acid
and propionic acid bacteria were introduced in the
urinary bladder of 15 patients in 2001 and 30 patients
in 2002 (group 1) with the durability of 30-40 minutes
within 7-8 days. 15 and 20 patients accordingly (group
2) were treated without the use of eubiotic.
The positive effect - absence of seeding of
pathogenic microbes - was achieved in 73,3-80,3% of
patients (group 1) and in 20,6-22,0%) of patients
(group 2). So the use of eubiotic in case of
"catheter-associated" infection in patients with benign
hyperplasia of prostate allows to increase the
effectiveness of the standard antibacterial treatment by
2,5-3,7 times.
Besides we have tested the effectiveness of the
use of the association of lactic acid and propionic acid
bacteria in treatment of patients with chronic
calculous pyelonephritis.
As the result of bacteriological analyses of urine
in 27 patients from urolithiasis department the
following was determined: E. coli in 25% of patients,
P. vulgaris, P.rettgeri - in 24%, P.aeruginosa -in 18%,
Citrobacter sp. - in 8%, Enterococcus sp. - in 11%,
Staphylococcus epidermidis, Staphylococcus
haemolyticus - in 7%, fungi of Candida type - in 7%.
In the course of treatment 15 patients took
eubiotic as oral dosing 20 minutes before the meal for
10 days together with antibacterial therapy. In the
control group (12 patients) antibacterial therapy with
antibiotics was only used.
After 10 days of therapy 87% of the patients in the
test group needed urine sanation. In 13%) of the
patients had seeding of P.aeruginosaup to 104
microbial cells/ml, but the culture resistance against
the used antibiotics reduced. In the control group in
65% patients the titre of the seeded microorganisms
remained from 104
to 106
microbial cells/ml.
The study of that association was carried out with
the participation of 15patients withurolithiasis
complicated by chronic pyelonephritis
withstaphylococcal, proteus, candidosisdysbacteriosis
of bowels. The quantity of those microorganisms
came to 107
COE/g. Probiotic was taken enterally as
7day-courses of treatment (15 minutes before the
meal). Daily dose was 50 ml of living bacterial cells.
No antibiotics were taken during the treatment.
Complete recovery was after the third treatment
course. At that the level of normal symbiotic flora
increased. Hemolytic strains of enterobacteria and
staphylococcus, as well as Proteus and fungi of
Candida type were not registered.
So, the received results testify the high
antagonistic activity in vivo of the selected association
of lactic acid and propionic acid bacteria. It gives the
foundation to recommend the association for wide use
in the complex therapy of infection diseases of urinary
tract [12].
For the prophylaxis and treatment of tuberculosis
we have selected the strains of lactic acid bacteria
possessing bactericidal activity against
MicbacteriumB5. The most active antagonists against
the mentioned test-culture were L. cellobiosus7n
(growth zone - 19 min), S.faecalis48n, L.brevis65n
andL.plantarum1 (17mm), L.fermentum175-2,
L.curvatus18d, L.casei261 (15mm). Antagonistic
activity of lactobacteria against the given
test-organism also depended on the culture medium.
When growing in milk the antagonism was revealed in
less degree.
The selected strains of lactobacteria are stable
against a number of antibiotics used for treatment of
tuberculosis. And these data prove the possibility to
use them in complex therapy of the mentioned disease.
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Comparative study of lactobacteria antagonistic
activity against five reference strains of brucella has
revealed six active cultures: L.brevis65n (28mm),
L.salivarius8d (25mm), L.fermentum17 (27mm), 7n
(25mm), 175-2 (20mm), and 27(24mm). Their
antagonistic activity against brucella of all kinds was
pronounced and varied only a little.
Chemotherapeutical activity of L.salivarius8d
strain, active in vitro in dilution as 1:10000, was tested
in vivo on 40 outbred white mice infected with
subcutaneous introduction of B. abortus54. The mice
in comparative group were injected with gentamicin.
At that the mice were divided into 8 equal groups
where the following periods were different: periods of
treatment with gentamicin injected intramuscularly;
periods of using lactic acid bacteria (4% of fodder
mass) and periods of cutting with necessary
bacteriological studies of 8 internals. The animals in
the control group were not treated after the infection.
The studies showed that infection indices and
intensity of internals contamination in comparison
with the control group were for sure lower in the first
and second groups in which lactic acid bacteria were
added to food within 5 days before the mice were
infected with brucella. In the third group in which
lactic acid bacteria were added to food within 20 days
after the infection and in group 7 in which the mice
were treated with gentamicin for 10 days in 20 days
after the contamination.
The test results testify that antagonistic activity of
L.salivarius8d in vivo against brucella is highly
competitive with gentamicine in action. This fact
proves the possibility to use that strain of lactic acid
bacteria for treatment and prophylaxis of brucellosis
[13].
We have carried out the test of eubiotic developed
by us –«Plantafermin» (FS RK42-53 5-2003) - in
treatment of gynecological diseases.
Use of antibiotics and sulfanamides for treatment
of those diseases especially in case of recurrents of
chronic diseases is ineffective and can create
complications as a result of impairment of
physiological status of macroorganism, reduction of a
number of antagonists of vaginal autoflora, because,
as a rule, exacerbation iscaused by activation of
autoinfection rather than reinfection. In this
connection we studied the influence of the new
eubiotic«Plantafermin» on the dynamics of
inflammatory gynecological diseases, therapeutic
effectiveness of this preparation in treatment of 135
women at the age of 16-48 years with inflammatory
diseases of genital tract. It was intravaginal
introduction of the preparation on cotton-gauze
tampon (bolsters) once a day for 7 days. The
exposition time was 2-3 hours. The control group
consisted of 20 women who took standard drug
treatment.
In the course of treatment microscopy of smears
and bacteriological study of-pathologic vaginal
discharge were carried out on the basis of standard
methods.
61 of the examined women had colpitis, 9 -
endocervicitis and 2 - adnexitis which was presented
mainly as recurrent of chronic form. 8 women with
adnexitis had abnormal menstruation cycle. In 27
women the inflammation affected uterine appendages
and vagina and in 9 women - vagina and cervical
canal. The control group consists of women with
mixed pathology.
Bacteriological study of vaginal discharge
revealed in patients with inflammatory gynecological
diseases evident disbiotic disturbances of vaginal
microbiocenose. In 90-95% patients there was great
deficiency of lactic-acid flora.
At that the vagina was contaminated with
conditionally-pathogenicmicroorganisms. Colpitis
was associated mainly with fungi of Candida type
(80%),pathogenic enterococcus (75%), and
hemolyzing strains of colibacillus (60%). In
case of adnexitis and mixed pathology pathogenic
colibacillus and fungi ofCandida type were seeded in
most patients. Pathogenic staphylococcus and
enterococcus were seeded in 20% of patients with
adnexitis and in 40% - withmixed pathology.
In case of end ocervicitis
conditionally-pathogenic microorganisms were
presented by hemoly zing enterococcus, often in
association with colibacillus. They were seeded in 57
% of examined women.
After the course of treatment the quantity of
leucocytes in vaginal discharge reduced. The
discharge was moderate, painful sensation and
hyperemia were not registered. Gram-positive bacilli
prevailed in the vaginal discharge. Coccal flora was
presented mainly by pathogenic streptococci. In the
patients with colpitis and endocervicitis the
colibacillus and yeast were seeded in single instances.
Colibacillus was seeded in 13,3% of patients with
adnexitis, the yeast was seeded in 33%. The yeast in
small quantity appeared in 15% of patients with mixed
pathology.
When using eubiotic«Plantafermin» by the
patients with inflammatory gynecological diseases,
positive clinical dynamics appeared after 3-5
procedures, vaginal flora became normal. The patients
noted good tolerance of the preparation and absence of
side effects.
In the control group where the traditional therapy
was used, the therapeutic effect appeared later (in
some cases the disease state was observed in 14-20
days), and the frequency of side effects came to 20%.
Significant improvement in microbiocenose was not
registered. All the patients from the control group had
great deficiency of lactobacilli. Frequency of seeding
of conditionally-pathogenic microorganisms
decreased. But that index is less pronounced in
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comparison with the patients taking «Plantafermin».
The researches revealed inflammatory diseases
ofgenital tract in women of childbearing age. At that
in 67% of cases the patients belonged to the age group
not older than 25. Associate character of etiological
factor and great percent of patients with yeast colpitis
demanding prolonged treatment are alarming
symptoms. Use of traditional drug therapy promotes
quick elimination of pathologic factor including
conditionally-pathogenic microorganisms. However
in this case the deficiency of symbiotic microflora, the
leading factor of colonizational resistance of the
organism is preserved and in some cases is even
redoubled. Absence of this barrier leads to repeated
contamination of-the genital tract or to the
development of endoinfections caused by
conditionally-pathogenic microorganisms and,
especially, by yeast.
Sufficient reduction of lactic acid bacteria in the
vaginal discharge in case of intensive development of
pathogenic flora puts a question on using of bacterial
preparations. The attempt to use a new eubiotic
«Plantafermin» in correction therapy has
demonstrated its effectiveness in case of treatment of
inflammatory process in genital tract and flora
normalization [14].
So lactic acid bacteria and their associations
possess a wide spectrum of antimicrobial action. This
testifies about the possibility of their wider use in
treatment not only enteric infections but also diseases
not related to gastrointestinal tract.
REFERENCES:
[1.] D.E. Corrieretal., Competitive exclusion of
Salmonella enteritidisin Leghorn Chicks,
Avian Diseases,38, 1994, 297-303.
[2.] L. Afanaciev, V. Gushul,G. Matrova et al.
Use ofLactovit-K probiotic for prophylaxis
and treatment of calves with gastrointestinal
disturbance, Epizootology and prophylaxis of
contagious diseases in agricultural animals,
1997,12.
[3.] N.N., Gavrilova, I.A. Ratnikova,
L.M.Lukasheva, T.B. Zhangeldin
"Polilactovit" preparation for prophylaxis
and treatment of gastrointestinal disturbances
in agricultural animals, patent № 6164, 1998,
2.
[4.] G.F.Bovkun, V.F.Semenikhina,
N.D.Tkatchev, Therapeutic action of
bifinorm in case of microecological
disturbances if internals in calves,Veterinary
medicine,3, 1999, 42-43.
[5.] I. Blaznik, I. M. Kumel. et
al,Sdravljenjekronicnegranulomotoznebolez
ni z acidofilnemmlecom, Zdrav. Vestn., 45,
77-79.
[6.] E. Tihole,Fisioloskipomenbacteriemije z
jejunalnomicroflora,Ibid.,51, 1982, 3-5.
[7.] V.V. Smirnov, S.R.Reznicet al., Discussion
matters on use of bacterial preparations for
correction of microflora of warm-blooded
animals,Microbiology journal, 54, 1992,
83-91.
[8.] K.M.Dekhiunyan, A.V. Avakyan,K.M.
Ambartsunyan et al. Experimentalgrounds on
the use of metabolic products of lactic-acid
bacteria for septic wounds treatment.
Postoperative complications: articles -
Erevan, 1986; 40-42.
[9.] A.Ostergaard, K.R.Embare et al.
Characterization of anti-listerial lactic acid
bacteria isolated from Thai fermented fish
products. Food Microbial 1998;
15;2;P.223-233.
[10.] K.Bayakisheva ,I.A.Ratnikova ,
N.N.Gavrilova , Study of antiinterferon
activity of microorganisms. The Izvestiya
Academy of Sciences Republic of
Kazakhstan Med. and Biol. 2001; 3-4;
P.23-25.
[11.] N.S.Egorov Foundations of the doctrine on
antibiotics (M.: Science, 1986).
[12.] L.S.Bectasova, I.A.RatnikovaUseeubiotics
in the therapy of infection diseases of urinary
tract.,сollected articles of 3-Annual
Conference young scientist-medical. Almaty,
2001.
[13.] N.N. Gavrilova, I.A. Ratnikova , T.A.
GrushinaAntagonistic activity of lactic acid
bacteria against pathogenslocaled outside the
gastrointestinal tract, Antibiotics and
chemotherapy, 48;2, 2003, 13-15.
[14.] B.B.Kostenko, L.B.Mishailova,
N.N.Gavrilova Use eubiotics plantaferminin
the therapy of infection diseases of
gynaecology,Medical. Almaty,2000, 53-54.