The presence of high concentration of toxic heavy metals in industrial waste directly leads to contamination of receiving soil and water bodies and has deleterious impact on both human health and aquatic life. In the present study samples from textile mill effluent from different areas of Jessore city were analyzed for the identification and characterization of bacteria which shows tolerance to Copper, Mercury and Zinc. The bacterial isolates were characterized on the basis of their morphological and physiological studies including size and shape of the organisms, arrangement of the cells, presence or absence of spores, regular or irregular forms, gram reaction, cultural characteristics, IMViC test, H 2S production, nitrate reduction, deep glucose agar test etc. All the bacterial isolates belonged to 3 genera Bacillus, Enterobacter and Pseudomonas. All the gram positive isolates used in our study showed highest level of tolerance to Zn and moderate level of tolerance to Cu while gram negative isolates showed higher tolerance to Zn in comparison with Cu in nutrient broth. But all of the isolates showed almost no tolerance to Hg. So, our bacterial isolates have the probability to use in the treatment of industrial effluent containing heavy metals and thus pollution due to heavy metal can be controlled. The goal of this study was to identify heavy metal tolerant bacteria from the textile effluent. This kind of study is very significant for broader investigation to obtain data about metal tolerant bacteria considering their potential use for bioremediation and about the interactions between metals and bacteria.
Removal of Lead Ion Using Maize Cob as a BioadsorbentIJERA Editor
The intensification of industrial activity and environmental stress greatly contributes to the significant rise of
heavy metal pollution in water resources making threats on terrestrial and aquatic life. The toxicity of metal
pollution is slow and interminable, as these metal ions are non bio-degradable. The most appropriate solution for
controlling the biogeochemistry of metal contaminants is sorption technique, to produce high quality treated
effluents from polluted wastewater. Maize cob readily available was used as sorbent for the removal of lead ions
from aqueous media. Adsorption studies were performed by batch experiments as a function of process
parameters such as sorption 500ppm,2.5g, 400minutes, 400 rpm and 5 PH. Concentration, Dosage, time, rpm,
and pH. I have found that the optimized parameters are Freundlich model fits best with the experimental
equilibrium data among the three tested adsorption isotherm models. The kinetic data correlated well with the
Lagergren first order kinetic model for the adsorption studies of lead using maize cob. It was concluded that
adsorbent prepared from maize cob as to be a favorable adsorbent and easily available to remove the heavy
metal lead (II) is 95 % and can be used for the treatment of heavy metals in wastewater.
Evaluation of Cadmium tolerant Fungi in the dying Staff and their removal Pot...AI Publications
Cadmium tolerance and bioremediation capacity of seven isolates including Aspergilus versicolor, Aspergillus fumigatus, Paecilomyces sp.9, Paecilomyces sp.G, Terichoderma sp, Microsporum sp,Cladosporium sp were determined. Minimum inhibitory concentration values among 2,000-6,000 mg lˉ1proved great ability of isolated strains to survive in cadmium polluted environments. The most tolerant fungi, Aspergilus versicolor, showed tolerance index of 0.93 in 100 mg lˉ1 cadmium agar media. Fungal resistance against cadmium is depended directly on strain’s biological function. A. versicolor was found to bioaccumulation over 7.67mg of cadmium per 1 g of mycelium, followed by 6.546, 6.354, and 6.286, 6.134 by Paecilomyces sp, Aspergilus fumigatus, Microsporum sp and Terichoderma sp, respectively. It can be noted that tolerance of the strains appears to be independent from bioaccumulation capacity. Finally, the results indicated that A. versicolor could be a prospective candidate for bioremediation processes.
Objectives :-
* Characterization and study of biochar.
*To examine biochar adsorption capacity.
*To determine the effect of KMnO4 as modifying agent on biochar adsorption.
Multidrug resistance pattern of bacteria isolated from domestic and tannery w...iosrjce
The objective of this study was to screen for the microorganisms in the domestic wastes and tannery
effluents and characterize it’s antibiogram to know the prevalence of resistant pathogens. A total of 109 isolates
of 16 different genera were isolated from 38 samples by aerobic culture method. Fourteen and ninety-five
isolates were obtained from six domestic wastes and 32 tannery waste samples, respectively. The isolates
belonged to the genus Micrococcus (18.3%), Alcaligens (15.6%), Staphylococcus (11.0%), Enterobacter (4.6%),
Shigella (14.7%), Klebsiella (6.4%), Haemophilus (4.6%), Citrobacter (3.7%), Actinobacillus (3.7%),
Escherichia (4.6%), Corynebacterium (4.6%) and others (8.2%). It was interesting to notice that most of the
isolates were Gram-negative bacillus (63.3%) and few were Gram-positive cocci (36.7%). Pathogenic
microorganisms from domestic wastes and tannery effluents have been identified and reported. Most of the
isolates were resistant to chloramphenicol, nalidixic acid, nitrofurontoin and cefixime. Levofloxacin and
imipenem were effective against 108 (99.5%) of the isolates. Multi drug resistance was observed in most of the
isolates. Some isolates were found in both domestic and tannery waste samples, but their antibiotic resistance
patterns were not similar. Serratia spp. and two Bacillus spp. with different antibiogram pattern were found only in tannery waste samples. The significant number of Multiple Antibiotic Resistant (MAR) bacteria was observed in both the samples. Human infections caused by these bacteria could be difficult to treat with available drugs.
Removal of Lead Ion Using Maize Cob as a BioadsorbentIJERA Editor
The intensification of industrial activity and environmental stress greatly contributes to the significant rise of
heavy metal pollution in water resources making threats on terrestrial and aquatic life. The toxicity of metal
pollution is slow and interminable, as these metal ions are non bio-degradable. The most appropriate solution for
controlling the biogeochemistry of metal contaminants is sorption technique, to produce high quality treated
effluents from polluted wastewater. Maize cob readily available was used as sorbent for the removal of lead ions
from aqueous media. Adsorption studies were performed by batch experiments as a function of process
parameters such as sorption 500ppm,2.5g, 400minutes, 400 rpm and 5 PH. Concentration, Dosage, time, rpm,
and pH. I have found that the optimized parameters are Freundlich model fits best with the experimental
equilibrium data among the three tested adsorption isotherm models. The kinetic data correlated well with the
Lagergren first order kinetic model for the adsorption studies of lead using maize cob. It was concluded that
adsorbent prepared from maize cob as to be a favorable adsorbent and easily available to remove the heavy
metal lead (II) is 95 % and can be used for the treatment of heavy metals in wastewater.
Evaluation of Cadmium tolerant Fungi in the dying Staff and their removal Pot...AI Publications
Cadmium tolerance and bioremediation capacity of seven isolates including Aspergilus versicolor, Aspergillus fumigatus, Paecilomyces sp.9, Paecilomyces sp.G, Terichoderma sp, Microsporum sp,Cladosporium sp were determined. Minimum inhibitory concentration values among 2,000-6,000 mg lˉ1proved great ability of isolated strains to survive in cadmium polluted environments. The most tolerant fungi, Aspergilus versicolor, showed tolerance index of 0.93 in 100 mg lˉ1 cadmium agar media. Fungal resistance against cadmium is depended directly on strain’s biological function. A. versicolor was found to bioaccumulation over 7.67mg of cadmium per 1 g of mycelium, followed by 6.546, 6.354, and 6.286, 6.134 by Paecilomyces sp, Aspergilus fumigatus, Microsporum sp and Terichoderma sp, respectively. It can be noted that tolerance of the strains appears to be independent from bioaccumulation capacity. Finally, the results indicated that A. versicolor could be a prospective candidate for bioremediation processes.
Objectives :-
* Characterization and study of biochar.
*To examine biochar adsorption capacity.
*To determine the effect of KMnO4 as modifying agent on biochar adsorption.
Multidrug resistance pattern of bacteria isolated from domestic and tannery w...iosrjce
The objective of this study was to screen for the microorganisms in the domestic wastes and tannery
effluents and characterize it’s antibiogram to know the prevalence of resistant pathogens. A total of 109 isolates
of 16 different genera were isolated from 38 samples by aerobic culture method. Fourteen and ninety-five
isolates were obtained from six domestic wastes and 32 tannery waste samples, respectively. The isolates
belonged to the genus Micrococcus (18.3%), Alcaligens (15.6%), Staphylococcus (11.0%), Enterobacter (4.6%),
Shigella (14.7%), Klebsiella (6.4%), Haemophilus (4.6%), Citrobacter (3.7%), Actinobacillus (3.7%),
Escherichia (4.6%), Corynebacterium (4.6%) and others (8.2%). It was interesting to notice that most of the
isolates were Gram-negative bacillus (63.3%) and few were Gram-positive cocci (36.7%). Pathogenic
microorganisms from domestic wastes and tannery effluents have been identified and reported. Most of the
isolates were resistant to chloramphenicol, nalidixic acid, nitrofurontoin and cefixime. Levofloxacin and
imipenem were effective against 108 (99.5%) of the isolates. Multi drug resistance was observed in most of the
isolates. Some isolates were found in both domestic and tannery waste samples, but their antibiotic resistance
patterns were not similar. Serratia spp. and two Bacillus spp. with different antibiogram pattern were found only in tannery waste samples. The significant number of Multiple Antibiotic Resistant (MAR) bacteria was observed in both the samples. Human infections caused by these bacteria could be difficult to treat with available drugs.
Introduction
The food and water contamination with heavy metals is increasing due to the environmental pollutions. Heavy metals are the elements with the density of more than 5 g/cm3 and have become a serious problem as a result of the urbanization and industrialization. These toxic metals pollute water, soil, plants, and eventually foodstuffs and our bodies. Several methods exist to remediate heavy metal pollution in waters such as membrane filtration, ion exchange mechanisms, or by precipitation. Yet, these techniques are not cost effective, in some cases, and do produce wastes that need to be properly disposed of. Microbial bioremediation could be an alternative. The use of microbes for remediation of heavy metals has been well studied. Some microorganisms, especially soil bacteria, have the ability to tolerate these contaminants. In addition, certain bacterial strains are capable of binding to heavy metals or transforming them into less toxic forms. Low operating costs, usable in foodstuffs, selective removal for specific toxic metals, minimal use of chemicals (resulting in low sludge production) and high efficiencies at very low levels of heavy metals are some of the advantages of biosorption methods. In this regard, the purpose of this study was to investigate the ability of active and passive absorption of heavy metals by a number of Lactic Acid Bacteria (LAB) strains in laboratory environment and food.
Materials and Methods
Seven LAB isolates including Lacticaseibacillus casei (RTCC 1296-3), Lacticaseibacillus rhamnosus (RTCC 1293-2), Lactiplantibacillus plantarum (RTCC 1290), Limosilactobacillus fermentum (RTCC 1303), Enterococcus faecium (RTCC 2347), Lactobacillus helveticus (RTCC 1304) and Lactobacillus acidophilus (RTCC 1299) were obtained from Razi type culture collection (RTCC), located at Razi vaccine and Serum Research Institute, Iran. All isolates were cultured in MRS (Scharlau, Spain) broth medium, at 37 °C for 24 hours, under anaerobic conditions. Pure cultures were preserved for long term by freezing at -70°C with 20% Glycerol. Heavy metals including Nitrate of Pb (II), Cd (II) and Ni (II) were purchased from Merck (Darmstadt, Germany). All standard solutions were prepared from the stock solutions containing 1000 mgl-1 in distilled water. Other chemicals used in study including Nitric acid (65%) and Hydrogen peroxide (37%), were also purchased from Merck, Germany. This study was conducted in two in- vitro and in-vivo phases; in the in- vitro phase, seven strains of bacteria with probiotic properties (L. casei, L. rhamnosus, L. plantarum, L. fermentum, Ent. facium, L. helveticus and L. acidofilous) were screened and then their ability to bind to cadmium (Cd), Lead (Pb) and nickel (Ni) in aqueous solution was investigated. Then, in the in-vivo stage, three probiotic strains that had the highest biosorption efficiency in the previously stage were selected and their effect with a ratio of 1:1:1 and contact time of 15 and 30 min
Isolation and Characterization of Nickel Tolerant Bacterial Strains from Elec...Agriculture Journal IJOEAR
Abstract— In the present study, an attempt was made to isolate and characterize nickel tolerant bacterial strains from the electroplating effluent contaminated soil. The effluent sample was collected at the direct outlet of electroplating industry and analyzed for physico-chemical characteristics such as pH (6.5), temperature (33), electrical conductivity (15.1 ms/cm), total solids (2309mg/l), total dissolved solids (5573 mg/l), chloride (0.20mg/l), sodium (0.13ppm), calcium (2.23ppm), potassium (0.20ppm), Biological Oxygen Demand (4200mg/l), Chemical Oxygen Demand (5243 mg/l) and nickel (4.063ppm). Enumeration of total bacterial population from the electroplating effluent contaminated soil sample was made in nutrient agar medium. Sixteen bacterial colonies were selected based on their abundance growth all of them were identified through morphological and biochemical characteristics. All the sixteen bacterial isolates were screened for its metal tolerance using nutrient agar medium incorporated with nickel metal. Based on the better growth performance, six bacterial strains were selected as potential metal tolerant organism. The selected metal tolerant bacterial strains were further characterized in the various environmental conditions such as pH (5, 7 & 9) temperature (5°C, 28°C, 37°C & 45°C) and concentration of metal ions (100ppm, 200ppm, 300ppm & 400ppm) for 5 days. The result reveals that one bacterial strain, Pseudomonas sp 1 was showed better growth in nickel metal based medium with pH 7 at 37°C temperature.
Study of Biocidal Activity of Copper A Reviewijtsrd
Copper ions, either alone or in copper complexes, have been used to disinfect liquids, solids and human tissue for centuries. Today copper is used as a water purifier, algaecide, fungicide, nematocide, molluscicide as well as an anti bacterial and anti fouling agent. Copper also displays potent anti viral activity. We have explained i the biocidal properties of copper ii the possible mechanisms by which copper is toxic to microorganisms and iii the systems by which many microorganisms resist high concentrations of heavy metals, with an emphasis on copper. Health care associated infections HAIs are a global problem associated with significant morbidity and mortality. Controlling the spread of antimicrobial resistant bacteria is a major public health challenge, and antimicrobial resistance has become one of the most important global problems in current times. The antimicrobial effect of copper has been known for centuries, and ongoing research is being conducted on the use of copper coated hard and soft surfaces for reduction of microbial contamination and, subsequently, reduction of HAIs. Dr. Pragya Tank "Study of Biocidal Activity of Copper: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-5 , August 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50696.pdf Paper URL: https://www.ijtsrd.com/chemistry/other/50696/study-of-biocidal-activity-of-copper-a-review/dr-pragya-tank
Parametric Studies on Detergent Using Low Cost SorbentIOSR Journals
: Water is a valued natural resource for the existence of all living organisms. Indian rivers are
polluted due to the discharge of untreated sewage and industrial effluents. Management of the quality of this
precious resource is, therefore, of special importance. In these study industrial effluents samples from the
various detergent factories were collected and analyzed for physicochemical and bacteriological evaluation of
pollution. The use and effectiveness of granular and powder activated carbon made from agricultural waste i.e.
coconut husk and that coconut husk is a suitable adsorbent for such an effluent. Maximum adsorption capacity
is a derived from Langmuir isotherm. A series of fixed bed experiments was carried out and the results were
applied to a bed-depth/service time model for column adsorption. The validity of such a model is discussed. In
the removal of organic matter in wastewater effluents from a industry waste water were investigated. The effect
of process variables such as carbonization temperature, carbonization duration and activation temperature on
the production and quality of activated carbon was studied as well as adsorption capacity was studied
Phylogenetic Analysis of the Potential Microorganism for Remediation of Heavy...CSCJournals
The present research work has been carried out to study the waste disposal contaminated site for its physico chemical and microbial characterization and identification of potential microorganism capable of bioaccumulation and biodegradation of heavy metals. The ambient conditions present in the metal contaminated environment shows the values: pH(5.4),temperature(30°C), moisture(11.71%), nutrients; Nitrogen(0.2mg/l), phosphorus(22.65mg/l) and sulphur(559.3mg/l) respectively. The biological parameters studied indicate Dissolved oxygen (7.4mg/l), Biological oxygen demand (3.8 mg/l), Chemical oxygen demand (64.6 mg/l). The microbial consortium identified was found to survive and multiply in the present environmental conditions. Microbial consortium was sequenced and compared using Bioinformatics tools like BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentrations of heavy metals viz 5mg/l, 25mg/l, 50mg/l, 100mg/l up to 800mg/l with special reference to Iron. At a concentration of 500mg/l, only one microorganism was found survived and multiplied. This shows that potential microorganism was only survived at higher concentration of iron. The 16SrRNA sequence and phylogenetic tree characterized the organism as Klebsiella pneumoniae, which was also confirmed by biochemical tests. The potential microorganism identified by BLAST technique can be used for remediation of the heavy metal from contaminated environment.
DOI:10.21276/ijlssr.2016.2.4.19
ABSTRACT- Halophiles, the most predominant organisms found in the mangrove forest, include halophilic bacteria in different environment such as salt lakes, saline soils and salted food. The majo rviatyri eodf hgaelnoeprhai loicf pmriocproerotrigesa nwishmicsh s tfuadciielidta steo iftasr upsreo dwuicteh ccoommpmoeurncdias l waiitmh sg. rIena tt hpiost esntutidayl ionf inSduunsdterirabla pnr osoceils,s faoncdu st hheays hbaeveen pmhaydsieo loong itchael iasnodla wtieorne oufs ehda lfooprh pilhiyc soiorgcahnemismicsa la anndd t mheiicrr ochbaioralocgteicriasla atinoanl.y sSiosi. lQs uwaelritea tciovlel escctreede nfirnogms foofu trh ed iifsfoelraetnets pwlaecree sd oofn eS uanndd etrhbraene aCmhaornagc tethriesmat iowna so f stehlee citseodl ahteasv iwnegr em doodneer aatneldy bgaoseodd ognr o1w6Sth rRwNhiAch g ewnaes sefuqrutehnecr inogp tpimhyisloegde nine tidci ftfreeree nwt egrer ocwotnhs trmucetdeida.. aTsh we eisllo laast evsa rsihoouws eedx tmrauceltlilpullea rh eenazvyym mee ptarol dtoulcetriaonncse t haenrde bayn teixbpiolotirci nrge stihsetairn cues.a gTeh efo or rvgaarnioisums sb iwoeterceh fnuortlohgeric taels pteudr pfoosr eEs.P S Key-words- Sundarban, Halophiles, EPS, NaCl
Dissertation ppt biostimulation- a potential practice for wastewater treaat...Sumer Pankaj
Phycoremediation is a green technology that supports the direct use of living green microalgae for in situ, or in place removal, degradation, of contaminants in soils, sludge, sediments, surface water and ground waters by the mechanisms of bio-transformation, bio-accumulation, bio-concentration, bio-sparging.
It can be said by the current study that microalgae has a great potential for the treatment of industrial and municipal wastewaters as compared to the chemical treatments available commercially. Biological systems are much more efficient in cleaning the excess nutrients from the waste water followed by generation of valuable biomass which can be applied in the food, fertilizer, energy production as use of inorganic chemicals like lime and ferrous sulphate generates huge amount of sludge in textile industries, but on the other hand static anaerobic treatment using acclimatized MLSS gives better colour reduction with zero sludge generation. Microalgal cells can be used in free form to treat waste waters containing high C.O.D., high ammonical nitrogen and high TDS. It not only provides a better reduction of chemicals from wastewaters but it also helps to reduce the operational cost of ETP. Microalgaes not only helps to remediate industrial waste waters but also to treat sweage water and to restore natural water bodies like lakes and ponds. As they are active in remediating the chemicals but also it shows an antagonistic effect against some pathogenic germs like total coliforms and fecal coliforms.
These microalgal cells can also be combined with bacterial biomass of activated sludge process to develop an Algal-Bacterial consortium (ALBA) for better enhancement in the reduction of chemicals from the wastewaters as this symbiotic relation of algae and bacteria provides high satiability of the microalgae along with MLSS and faceable in terms of price and economy for instance the bacterial biomass provides carbon dioxide to algal cells for photosynthesis and in return the bacteria acquires oxygen from algae. The harvested biomass from the ETP’s can be used as bio-fertilizers as it consists of appropriate ratio of vital macro and micro nutrients like N,P,K etc. which enhance the growth of plantlets. It can also be used as aqua feeds for shrimps, fishes and molluscs. Furthermore these microlgal cells are non-toxic in the environment as it becomes a part of food chain and do not cause eutrophication. Therefore, micro-algal based treatment is most suitable for the treating the waste waters and restoring the natural water bodies as compared to other chemical treatments.
Fish to finance: unraveling the economic threads of Bangladesh’s Blue EconomyAbdullaAlAsif1
Bangladesh, with its extensive river network, vast coastlines, and rich maritime heritage, stands at the crossroads of a transformative Blue Economy. This comprehensive review explores the rich tapestry of Bangladesh's maritime journey. Commencing with a historical lens, it traces the nation's roots in fisheries, showcasing its transformation into a diversified and dynamic Blue Economy. The significance of this transition unfolds across the review, underscoring the sector's contributions to GDP, employment, and food security, while candidly addressing challenges such as overfishing and environmental impact. This work not only chronicles historical milestones but distills valuable lessons from both successes and setbacks, offering a nuanced understanding of the economic resilience embedded in Bangladesh's fisheries sector. Beyond a historical retrospective, the review navigates through the contemporary landscape, emphasizing diversification beyond fisheries. It illuminates economic opportunities in maritime trade, explores non-fish marine resources, and delves into the transformative role of aquaculture. The policy framework governing Bangladesh's Blue Economy comes into focus, dissecting government strategies, legal landscapes, and international collaborations. The review also champions environmental sustainability, highlighting the impact of Blue Economy activities on marine ecosystems and advocating for conservation measures, thus positioning Bangladesh as a steward of its rich maritime heritage. Financial mechanisms and investments emerge as pivotal elements, shaping the trajectory of Blue Economy projects, as evidenced by case studies that weave success stories and lessons learned into the narrative. Concluding with a forward-looking perspective, the study identifies emerging trends, growth opportunities, and strategies to tackle challenges, offering a comprehensive roadmap for sustainable development. In weaving together economic vibrancy, environmental stewardship, and future aspirations, this review illuminates Bangladesh's maritime odyssey as a beacon for the global Blue Economy community.
Successful hybridization between Clarias microstomus♂ and Clarias gariepinus♀AbdullaAlAsif1
Hybridization is a significant approach to breeding that enables the creation of new varieties that enhance genetic diversity. Through fish inter-species hybridization, it is possible to produce hybrids that can be utilized in aquaculture and stocking programs to enhance growth rate, transfer desirable traits between species, and combine the favourable attributes of two parents into a single progeny. The present study revealed the successful hybridization between Clarias microstomus (Ng, 2001) ♂ and Clarias gariepinus (Burchell, 1822) ♀ for the first time in the aquaculture industry. C. microstomus is a species of Clariid catfish endemic to the island of Borneo and C. gariepinus is an introduced species widely aquacultured throughout the southeast Asian region. The embryonic development of the hybrid offspring is described with the hatching percentage of 58.63% and the early survival rate for the first 72 hours for the larvae of 85.76%. The hybrids showed no signs of deformities and developed normally. The findings of the study provide a new high yield variety of catfish for farmers and aquaculture industry for increasing production and profit margin.
More Related Content
Similar to An investigation on heavy metal tolerance properties of bacteria isolated from textile effluent
Introduction
The food and water contamination with heavy metals is increasing due to the environmental pollutions. Heavy metals are the elements with the density of more than 5 g/cm3 and have become a serious problem as a result of the urbanization and industrialization. These toxic metals pollute water, soil, plants, and eventually foodstuffs and our bodies. Several methods exist to remediate heavy metal pollution in waters such as membrane filtration, ion exchange mechanisms, or by precipitation. Yet, these techniques are not cost effective, in some cases, and do produce wastes that need to be properly disposed of. Microbial bioremediation could be an alternative. The use of microbes for remediation of heavy metals has been well studied. Some microorganisms, especially soil bacteria, have the ability to tolerate these contaminants. In addition, certain bacterial strains are capable of binding to heavy metals or transforming them into less toxic forms. Low operating costs, usable in foodstuffs, selective removal for specific toxic metals, minimal use of chemicals (resulting in low sludge production) and high efficiencies at very low levels of heavy metals are some of the advantages of biosorption methods. In this regard, the purpose of this study was to investigate the ability of active and passive absorption of heavy metals by a number of Lactic Acid Bacteria (LAB) strains in laboratory environment and food.
Materials and Methods
Seven LAB isolates including Lacticaseibacillus casei (RTCC 1296-3), Lacticaseibacillus rhamnosus (RTCC 1293-2), Lactiplantibacillus plantarum (RTCC 1290), Limosilactobacillus fermentum (RTCC 1303), Enterococcus faecium (RTCC 2347), Lactobacillus helveticus (RTCC 1304) and Lactobacillus acidophilus (RTCC 1299) were obtained from Razi type culture collection (RTCC), located at Razi vaccine and Serum Research Institute, Iran. All isolates were cultured in MRS (Scharlau, Spain) broth medium, at 37 °C for 24 hours, under anaerobic conditions. Pure cultures were preserved for long term by freezing at -70°C with 20% Glycerol. Heavy metals including Nitrate of Pb (II), Cd (II) and Ni (II) were purchased from Merck (Darmstadt, Germany). All standard solutions were prepared from the stock solutions containing 1000 mgl-1 in distilled water. Other chemicals used in study including Nitric acid (65%) and Hydrogen peroxide (37%), were also purchased from Merck, Germany. This study was conducted in two in- vitro and in-vivo phases; in the in- vitro phase, seven strains of bacteria with probiotic properties (L. casei, L. rhamnosus, L. plantarum, L. fermentum, Ent. facium, L. helveticus and L. acidofilous) were screened and then their ability to bind to cadmium (Cd), Lead (Pb) and nickel (Ni) in aqueous solution was investigated. Then, in the in-vivo stage, three probiotic strains that had the highest biosorption efficiency in the previously stage were selected and their effect with a ratio of 1:1:1 and contact time of 15 and 30 min
Isolation and Characterization of Nickel Tolerant Bacterial Strains from Elec...Agriculture Journal IJOEAR
Abstract— In the present study, an attempt was made to isolate and characterize nickel tolerant bacterial strains from the electroplating effluent contaminated soil. The effluent sample was collected at the direct outlet of electroplating industry and analyzed for physico-chemical characteristics such as pH (6.5), temperature (33), electrical conductivity (15.1 ms/cm), total solids (2309mg/l), total dissolved solids (5573 mg/l), chloride (0.20mg/l), sodium (0.13ppm), calcium (2.23ppm), potassium (0.20ppm), Biological Oxygen Demand (4200mg/l), Chemical Oxygen Demand (5243 mg/l) and nickel (4.063ppm). Enumeration of total bacterial population from the electroplating effluent contaminated soil sample was made in nutrient agar medium. Sixteen bacterial colonies were selected based on their abundance growth all of them were identified through morphological and biochemical characteristics. All the sixteen bacterial isolates were screened for its metal tolerance using nutrient agar medium incorporated with nickel metal. Based on the better growth performance, six bacterial strains were selected as potential metal tolerant organism. The selected metal tolerant bacterial strains were further characterized in the various environmental conditions such as pH (5, 7 & 9) temperature (5°C, 28°C, 37°C & 45°C) and concentration of metal ions (100ppm, 200ppm, 300ppm & 400ppm) for 5 days. The result reveals that one bacterial strain, Pseudomonas sp 1 was showed better growth in nickel metal based medium with pH 7 at 37°C temperature.
Study of Biocidal Activity of Copper A Reviewijtsrd
Copper ions, either alone or in copper complexes, have been used to disinfect liquids, solids and human tissue for centuries. Today copper is used as a water purifier, algaecide, fungicide, nematocide, molluscicide as well as an anti bacterial and anti fouling agent. Copper also displays potent anti viral activity. We have explained i the biocidal properties of copper ii the possible mechanisms by which copper is toxic to microorganisms and iii the systems by which many microorganisms resist high concentrations of heavy metals, with an emphasis on copper. Health care associated infections HAIs are a global problem associated with significant morbidity and mortality. Controlling the spread of antimicrobial resistant bacteria is a major public health challenge, and antimicrobial resistance has become one of the most important global problems in current times. The antimicrobial effect of copper has been known for centuries, and ongoing research is being conducted on the use of copper coated hard and soft surfaces for reduction of microbial contamination and, subsequently, reduction of HAIs. Dr. Pragya Tank "Study of Biocidal Activity of Copper: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-5 , August 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50696.pdf Paper URL: https://www.ijtsrd.com/chemistry/other/50696/study-of-biocidal-activity-of-copper-a-review/dr-pragya-tank
Parametric Studies on Detergent Using Low Cost SorbentIOSR Journals
: Water is a valued natural resource for the existence of all living organisms. Indian rivers are
polluted due to the discharge of untreated sewage and industrial effluents. Management of the quality of this
precious resource is, therefore, of special importance. In these study industrial effluents samples from the
various detergent factories were collected and analyzed for physicochemical and bacteriological evaluation of
pollution. The use and effectiveness of granular and powder activated carbon made from agricultural waste i.e.
coconut husk and that coconut husk is a suitable adsorbent for such an effluent. Maximum adsorption capacity
is a derived from Langmuir isotherm. A series of fixed bed experiments was carried out and the results were
applied to a bed-depth/service time model for column adsorption. The validity of such a model is discussed. In
the removal of organic matter in wastewater effluents from a industry waste water were investigated. The effect
of process variables such as carbonization temperature, carbonization duration and activation temperature on
the production and quality of activated carbon was studied as well as adsorption capacity was studied
Phylogenetic Analysis of the Potential Microorganism for Remediation of Heavy...CSCJournals
The present research work has been carried out to study the waste disposal contaminated site for its physico chemical and microbial characterization and identification of potential microorganism capable of bioaccumulation and biodegradation of heavy metals. The ambient conditions present in the metal contaminated environment shows the values: pH(5.4),temperature(30°C), moisture(11.71%), nutrients; Nitrogen(0.2mg/l), phosphorus(22.65mg/l) and sulphur(559.3mg/l) respectively. The biological parameters studied indicate Dissolved oxygen (7.4mg/l), Biological oxygen demand (3.8 mg/l), Chemical oxygen demand (64.6 mg/l). The microbial consortium identified was found to survive and multiply in the present environmental conditions. Microbial consortium was sequenced and compared using Bioinformatics tools like BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentrations of heavy metals viz 5mg/l, 25mg/l, 50mg/l, 100mg/l up to 800mg/l with special reference to Iron. At a concentration of 500mg/l, only one microorganism was found survived and multiplied. This shows that potential microorganism was only survived at higher concentration of iron. The 16SrRNA sequence and phylogenetic tree characterized the organism as Klebsiella pneumoniae, which was also confirmed by biochemical tests. The potential microorganism identified by BLAST technique can be used for remediation of the heavy metal from contaminated environment.
DOI:10.21276/ijlssr.2016.2.4.19
ABSTRACT- Halophiles, the most predominant organisms found in the mangrove forest, include halophilic bacteria in different environment such as salt lakes, saline soils and salted food. The majo rviatyri eodf hgaelnoeprhai loicf pmriocproerotrigesa nwishmicsh s tfuadciielidta steo iftasr upsreo dwuicteh ccoommpmoeurncdias l waiitmh sg. rIena tt hpiost esntutidayl ionf inSduunsdterirabla pnr osoceils,s faoncdu st hheays hbaeveen pmhaydsieo loong itchael iasnodla wtieorne oufs ehda lfooprh pilhiyc soiorgcahnemismicsa la anndd t mheiicrr ochbaioralocgteicriasla atinoanl.y sSiosi. lQs uwaelritea tciovlel escctreede nfirnogms foofu trh ed iifsfoelraetnets pwlaecree sd oofn eS uanndd etrhbraene aCmhaornagc tethriesmat iowna so f stehlee citseodl ahteasv iwnegr em doodneer aatneldy bgaoseodd ognr o1w6Sth rRwNhiAch g ewnaes sefuqrutehnecr inogp tpimhyisloegde nine tidci ftfreeree nwt egrer ocwotnhs trmucetdeida.. aTsh we eisllo laast evsa rsihoouws eedx tmrauceltlilpullea rh eenazvyym mee ptarol dtoulcetriaonncse t haenrde bayn teixbpiolotirci nrge stihsetairn cues.a gTeh efo or rvgaarnioisums sb iwoeterceh fnuortlohgeric taels pteudr pfoosr eEs.P S Key-words- Sundarban, Halophiles, EPS, NaCl
Dissertation ppt biostimulation- a potential practice for wastewater treaat...Sumer Pankaj
Phycoremediation is a green technology that supports the direct use of living green microalgae for in situ, or in place removal, degradation, of contaminants in soils, sludge, sediments, surface water and ground waters by the mechanisms of bio-transformation, bio-accumulation, bio-concentration, bio-sparging.
It can be said by the current study that microalgae has a great potential for the treatment of industrial and municipal wastewaters as compared to the chemical treatments available commercially. Biological systems are much more efficient in cleaning the excess nutrients from the waste water followed by generation of valuable biomass which can be applied in the food, fertilizer, energy production as use of inorganic chemicals like lime and ferrous sulphate generates huge amount of sludge in textile industries, but on the other hand static anaerobic treatment using acclimatized MLSS gives better colour reduction with zero sludge generation. Microalgal cells can be used in free form to treat waste waters containing high C.O.D., high ammonical nitrogen and high TDS. It not only provides a better reduction of chemicals from wastewaters but it also helps to reduce the operational cost of ETP. Microalgaes not only helps to remediate industrial waste waters but also to treat sweage water and to restore natural water bodies like lakes and ponds. As they are active in remediating the chemicals but also it shows an antagonistic effect against some pathogenic germs like total coliforms and fecal coliforms.
These microalgal cells can also be combined with bacterial biomass of activated sludge process to develop an Algal-Bacterial consortium (ALBA) for better enhancement in the reduction of chemicals from the wastewaters as this symbiotic relation of algae and bacteria provides high satiability of the microalgae along with MLSS and faceable in terms of price and economy for instance the bacterial biomass provides carbon dioxide to algal cells for photosynthesis and in return the bacteria acquires oxygen from algae. The harvested biomass from the ETP’s can be used as bio-fertilizers as it consists of appropriate ratio of vital macro and micro nutrients like N,P,K etc. which enhance the growth of plantlets. It can also be used as aqua feeds for shrimps, fishes and molluscs. Furthermore these microlgal cells are non-toxic in the environment as it becomes a part of food chain and do not cause eutrophication. Therefore, micro-algal based treatment is most suitable for the treating the waste waters and restoring the natural water bodies as compared to other chemical treatments.
Similar to An investigation on heavy metal tolerance properties of bacteria isolated from textile effluent (20)
Fish to finance: unraveling the economic threads of Bangladesh’s Blue EconomyAbdullaAlAsif1
Bangladesh, with its extensive river network, vast coastlines, and rich maritime heritage, stands at the crossroads of a transformative Blue Economy. This comprehensive review explores the rich tapestry of Bangladesh's maritime journey. Commencing with a historical lens, it traces the nation's roots in fisheries, showcasing its transformation into a diversified and dynamic Blue Economy. The significance of this transition unfolds across the review, underscoring the sector's contributions to GDP, employment, and food security, while candidly addressing challenges such as overfishing and environmental impact. This work not only chronicles historical milestones but distills valuable lessons from both successes and setbacks, offering a nuanced understanding of the economic resilience embedded in Bangladesh's fisheries sector. Beyond a historical retrospective, the review navigates through the contemporary landscape, emphasizing diversification beyond fisheries. It illuminates economic opportunities in maritime trade, explores non-fish marine resources, and delves into the transformative role of aquaculture. The policy framework governing Bangladesh's Blue Economy comes into focus, dissecting government strategies, legal landscapes, and international collaborations. The review also champions environmental sustainability, highlighting the impact of Blue Economy activities on marine ecosystems and advocating for conservation measures, thus positioning Bangladesh as a steward of its rich maritime heritage. Financial mechanisms and investments emerge as pivotal elements, shaping the trajectory of Blue Economy projects, as evidenced by case studies that weave success stories and lessons learned into the narrative. Concluding with a forward-looking perspective, the study identifies emerging trends, growth opportunities, and strategies to tackle challenges, offering a comprehensive roadmap for sustainable development. In weaving together economic vibrancy, environmental stewardship, and future aspirations, this review illuminates Bangladesh's maritime odyssey as a beacon for the global Blue Economy community.
Successful hybridization between Clarias microstomus♂ and Clarias gariepinus♀AbdullaAlAsif1
Hybridization is a significant approach to breeding that enables the creation of new varieties that enhance genetic diversity. Through fish inter-species hybridization, it is possible to produce hybrids that can be utilized in aquaculture and stocking programs to enhance growth rate, transfer desirable traits between species, and combine the favourable attributes of two parents into a single progeny. The present study revealed the successful hybridization between Clarias microstomus (Ng, 2001) ♂ and Clarias gariepinus (Burchell, 1822) ♀ for the first time in the aquaculture industry. C. microstomus is a species of Clariid catfish endemic to the island of Borneo and C. gariepinus is an introduced species widely aquacultured throughout the southeast Asian region. The embryonic development of the hybrid offspring is described with the hatching percentage of 58.63% and the early survival rate for the first 72 hours for the larvae of 85.76%. The hybrids showed no signs of deformities and developed normally. The findings of the study provide a new high yield variety of catfish for farmers and aquaculture industry for increasing production and profit margin.
Freshwater gastropod diversity in the selected lotic environment, Betong, Sar...AbdullaAlAsif1
Freshwater Gastropoda can be considered as the biological indicator in ecosystems such as rivers, streams, lakes, and ponds. The objectives of this study are to identify freshwater gastropod species, diversity, and distribution along Sungai Penebak, Sungai Nanga Tiga, and Sungai Kabo in the Betong division. The study was conducted on 10 November 2020. A 50 m transect was laid on the riverbank of all three stations. The existing specimens were counted and collected from each sampling point and stored were taken to the laboratory for species identification. The diversity index and morphological study of freshwater gastropods were performed. Five species of freshwater gastropods belonging to four families were discovered. The five species of freshwater gastropods consisted of Sulcospira pageli, which shows great abundance, followed by Clea nigricans, Brotia costula, Pila ampullacea, and Vittina pennata. The diversity indices of collected Gastropoda species from the different stations, for instance, Shannon Weiner diversity index (H’), Pielou’s evenness index (J’), and Margalef’s richness index were assessed; where station 3 showed higher diversity of Gastropoda compared to other two stations. The information presented in this paper might be helpful for ecological wealth studies and considered as the baseline data for the stream ecosystem in Sarawak, Malaysia.
Macrofaunal diversity on the surface sediment of mangrove habitat adjacent to...AbdullaAlAsif1
Macrofaunal diversity and composition are essential to understanding the ecosystem structures and functions of mangroves in any dynamic estuarine coast. To understand the temporal distribution of macrofaunal community structures and diversity on the surface sediment in the intertidal mangrove forest next to the seagrass beds, this study was conducted at Punang-Sari-River estuary, Lawas, Sarawak (Malaysian Borneo), South China Sea. Sampling was done during post-monsoon, intermediate-September, premonsoon, and monsoon from July 2019 to February 2020. The mean macrofauna abundance was higher pre-monsoon, while Gastropoda Optediceros breviculum (L. Pfeiffer, 1855) was the most abundant (RA = 0.704). Important species index (ISI = 70.36) and percentage of contribution (64.97%) were also higher of this species than any other records from the site. The ANOSIM (Analysis of Similarity), Jaccard index and SIMPER (Similarity Percentage Analysis) analysis suggested that the highest species abundance similarity was observed between pre-monsoon and monsoon, while the highest dissimilarity was observed between intermediate-September and pre-monsoon (74.67%). Seasonal PCA, nMDS, and cluster analysis revealed that pre-monsoon and monsoon were comparable in species and individual abundance. The dominance (0.85) was found significantly higher (P < 0.0001) in intermediate-September, while Simpson (0.734), Shannon (1.56) and Evenness indices (0.528) were found higher in monsoon, followed by the higher Margalef richness indices (3.21) in post-monsoon. It was observed that the seasonal ecological parameters were found to be significantly (P<0.05) different, including the temperature, salinity, rainfall, pore water nitrogenous compounds (NO2, NO3 and NH3-N), phosphorus compound (PO4) and micro minerals. The Canonical Correspondence Analysis (CCA) results suggested that ecological factors influenced several species. Considering the few studies on mangrove macrofauna in Malaysian Borneo, further research on their trophic structures and food web interactions is very important to disclose their overall characteristics and ecosystem functions.
Population characteristics of the Japanese threadfin bream Nemipterus japonic...AbdullaAlAsif1
Nemipterus japonicus (Japanese threadfin bream) is one of the most significant fish species throughout the Indo-Pacific regions. No previous studies on the population dynamics of N. japonicus have been carried out in the Eastern Malaysia (Malaysian Borneo). The present study focused on the population dynamics of N. japonicus from April 2013 to March 2014 in the coastal area of Bintulu, South China Sea. Local fishermen provided samples, which were then analysed. The FiSAT II software was used to evaluate the collected length frequency data. The asymptotic length (L ∞) and growth coefficient (K) were 26.78 cm and 0.85 yr-1, respectively. The growth performance index (ϕ'), total mortality coefficient (Z), natural mortality (M) and fishing mortality (F) were 2.785, 2.97, 1.63 and 1.34 per year, respectively. According to the estimates, the exploitation rate (E) for N. japonicus was 0.45, while the E 0.1 , E 0.5 and E max were determined as 0.72, 0.4 and 0.806, respectively. The obtained value of exploitation was less than E max, although slightly higher than E 0.5. The recruitment characteristics of N. japonicus have been stable throughout the year, with one peak pulse. The results contribute to the knowledge of the dynamics of N. japonicus populations and are important for the sustainable management of the Sarawak fishing resources.
Proximate and mineral composition of the long-spined Sea Urchin (Diadema seto...AbdullaAlAsif1
Gonad of sea urchin is considered as food delicacy in many parts of the world due to high nutrition and mineral contents. Several species of sea urchins are available in Malaysia and reported from different habitat, including seagrass beds, coastal breakwater, coral reefs and other ecologically important habitats. The current study was undertaken to evaluate the proximate and mineral composition of long spined sea urchin (Diadema setosum) roe collected from breakwater of Terengganu, Malaysia in October 2019, November 2019 and January 2020. The samples were analysed for proximate including protein, lipid, moisture, and ash content of sea urchin roe. The macro, micro minerals and heavy metals were also evaluated including Ca, Fe, Zn, Cu, Co, Se, Mg, Ni, Pb, Al, and Cd. The result suggested that, long spined sea urchin (Diadema setosum) roe can be considered as good sources of food due to high percentage of protein (ranged between 36.21±0.44 and 50.14±4.63). The presence of heavy metal such as Ni, Pb, and Cd suggesting the breakwater environments were not good enough and the possible sources of heavy metals contamination in sea water environment must be stopped and eradicated. This study provides important information with regard to the proximate and mineral values of D. setosum, as well as the need for a better management of its habitat before D. setosum can be widely promoted as delicacy in this region.
Trends in seagrass research and conservation in Malaysian watersAbdullaAlAsif1
The seagrass ecosystems found in the marine and coastal areas, with substantial economic and ecological services and span all over the globe excluding the Antarctic region. The Coral Triangle and Southeast Asia are recognized as a worldwide hotspot of seagrass species and habitats, encompassing 10-21 species of seagrass in every nation , although the study, understanding, and quantity of publications on seagrass ecosystems are rather limited in the region, including Malaysia. Malaysia contains 18 seagrass species from three families, which occupy 16.8 km 2 of coastal area, where the study and discovery of seagrass species and meadows began in 1904 with the report of Beccari. All of the published papers reviewed reported on Malaysian seagrass-related research, which was divided into nine topic groups: biology and distribution, carbon sequestration, fauna, remote sensing, impact and pollution genetic study, restoration , microbiological investigation, and others. The extensive study of the seagrass ecosystem began in 1993, and we have identified 183 published papers from Scopus, 141 publications from Web of Science, and 42 from Google Scholar. However, the average trend of the number of publications from 1993 to 1999 was 0.71 ± 0.36, while from 2000 to 2022 was 7.70 ± 1.16 followed by the average trend of the yearly number of publications was 6.78 ± 1.08. The highest number of publications was found on faunal categories (43.17%), followed by biology and distribution (21.85%). The number of articles that were published on Malaysian seagrass meadows each year has been discovered to be rising, which indicates that the trends in seagrass study and publishing were progressively garnering the attention of researchers, academics, and the government. However, to better understand the sustainable ecology and ecosystem services provided by seagrass habitats, an emphasis on certain research niches, such as the genetic study of flora and fauna in seagrass meadows, microbial ecology, and restoration as well as conservation of seagrass species might be helpful.
First record of saucer scallop Ylistrum balloti (Bernardi, 1861) from equator...AbdullaAlAsif1
Ylistrum balloti is one of the Pectinidae species distributed within the Indo-Pacific region. Recently, 15 live specimens of Y. balloti were recorded from the continental shelf of Sarawak, Malaysia. The main morphological characteristics were displayed on the outer valve, these being a brown-red colour and a clear concentric pattern of thin brown lines. The prominent internal ribbing numbers on both valves also helped species identification. The preliminary report of Y. balloti revealed that the species is present in Sarawak waters and can be further explored in the future.
Culture and production of Lucilia sericata Meigen (1826) larvae for rearing s...AbdullaAlAsif1
The fly larvae (Lucilia sericata) are being used as an alternative protein source in any kind of animal feed as it reduces the cost of preparation of feed. This current study focused on the physico-chemical and proximate properties of raw poultry waste, while this study also revealed the production process of fly larvae and the proximate composition of it. The poultry waste was collected from Suvro poultry farm, Sutiakhali, Mymensingh, then the physic-chemical properties of the raw poultry waste were determined using different procedures. Three treatments were considered for production of fly larvae, T 1 (3 kg), T 2 (6 kg) and T 3 (9 kg) in a 15 kg capacity tray with three replications. After production, the proximate composition were also measured of fly larvae. Physico-chemical properties such as, color, odor, texture, temperature, pH, total solids (TSS+TDS), chemical oxygen demand, dissolved oxygen, alkalinity, available N, available P, and fiber in raw poultry waste were determined and presented. Proximate composition such as, moisture, total N, total P, available N, total Ca, ash and crude fiber were found in significant amount in the poultry waste. It was found that T 3 (1350±68g) produced highest volume of live maggot compared to the other treatments, but T 2 (17.50±1.10%) produced highest percentage volume. The proximate compositions of fly larvae were assessed and found 56.60±0.25% protein value in it, suggesting that fly larvae could be the protein replacer in fish feed. The result of this study revealed cheap protein source in aquaculture production, such as production and rearing of stinging catfish Heteropneustes fossilis, and the findings might be helpful for cost reduction in aquaculture operation.
Replacement of fish meal by Lucilia sericata (Meigen, 1826) live larvae and p...AbdullaAlAsif1
Reduction of aquaculture operations and production cost is key to the success and profitability of this enterprise, while protein needs, especially the cost of fish meal, are cery expensive. The current study was conducted to investigate the post-larval production performance of Heteropneustes fossilis using Lucilia sericata maggot as a fish meal replacer in two ways, namely, live larvae and powder form. A 28 days growth trial was performed where five isonitrogenous diets for Heteropneustes fossilis post-larvae were experimented using live maggots and maggot meal, respectively. The proximate composition of each formulated diet, growth parameters of fish post-larvae, such as, weight gain, specific growth rate, protein efficiency ratio, apparent protein utilization, survival rate and the food conversion ratio were examined. After the experiment, the carcass composition of the experimental fishes were evaluated. The best final weight (1.61 g), weight gain (1.418 g), percentage of weight gain (739±1.18%), specific growth rate (2.63), protein efficiency ratio (2.29), apparent protein utilization (85%), survival rate (90%) and lower food conversion ratio (2.06) were observed in fish fed with 75% maggot meal as a substitute of fish meal. This study will help the aquaculture industry, especially the catfish culture in identifying an alternate source of protein and lowering the cost of aquaculture operation.
The molecular approach reveals the relationship among Venus clams (Meretrix s...AbdullaAlAsif1
Molecular study is important to detect variations and similarities among species from the same genus, in case if they do not encompass any morphological or physiological differences. The study was conducted to differentiate among species of Meretrix spp. (Meretrix lyrata, M. meretrix, and M. lusoria) obtained from two locations in Malaysia through the phylogenetic tree. The adductor muscle tissues were used to extract DNA and to perform other procedures; the samples were subjected to analyses using PCR and gel electrophoresis. The multiple sequence comparison was conducted by MUSCLE and the phylogenetic relationships were established using Maximum Likelihood (ML) statistical methods with MEGA 6.0 statistical software. M. lyrata samples showed 99% similarity to the three accessions sequence, where M. lyrata indicated 87% similarities, and M. meretrix showed not more than 89% similarities from the deposited sequence. The nucleotide base composition sequences consisted of the mean of Thiamine (T) 37.9%, Cytosine (C) 15.4%, Adenine (A) 27.4%, and Guanine (G) 19.4%. Maximum Likelihood (ML) analysis was conducted using the Tamura 3-parameter model to establish five major clades on Meretrix spp. and two out-groups clades significantly different from the Meretrix spp. These major clades were closely related to each other at the 50% evidence of bootstrap, which grouped as genus Meretrix. The present study on Meretrix spp. from the Sarawak locality was able to differentiate COI sequences between M. lyrata, M. meretrix, and M. lusoria. M. lusoria was close related to M. meretrix with strong bootstrap supporting evidence at 96% scoring. Moreover, M. lyrata was inferred as the ancestor to M. meretrix, and M. lusoria from Sarawak, Malaysia.
Disappearance or overlooked or untouched? - A brief history of aquatic gastro...AbdullaAlAsif1
The aquatic gastropods (marine and freshwater) in Malaysian Borneo (MYB) have an ancient history of 255 years, while the first record was observed in 1767 by the pioneer Carl Linnaeus. Over time, many European, native taxonomists and conservationists have worked on the diverse group of gastropods in the Malaysian part of Borneo (states of Sabah and Sarawak). No previous work has been conducted to assess the historical status of this particular class of Mollusca. The study objective was to reveal the types of aquatic gastropods and the number of published research and expeditions conducted in MYB. The bibliometric analysis found 145 research publications mentioning the names of the region and reporting at least one gastropod species from this geographic region. Over the history of 255 years, a total of 559 gastropod species were reported by different research groups. Between 1767 and 1900, a total of 150 gastropod species were reported, followed by 79 species from 1900 to 2000 and 330 species from 2001 to 2022. The Grubbs test (p<0.05) identified outlier years for the reporting of gastropod species from MYB with 54 new species each in 2011 and 2020 and 39 species in 2001. Over the history of aquatic gastropod research in MYB, the taxonomists making the greatest contribution were Han Raven and his colleagues from Naturalis who reported 111 new species from the region, followed by Nur Leena Wong from Universiti Putra Malaysia (54 new species). The results suggested that reporting of new species from MYB is increasing, which may reflect the many unexplored sites and suggests the existence of as yet unrecorded species in this ecoregion. In this regard further expeditions and research should improve estimates of the true number of aquatic gastropod species in this area. These results can help the regional governments in making a regional biodiversity database as well as in taking decisions regarding aquatic gastropod research and management.
A ray of hope in the darkness: What we have learned from Yangtze giant soft-s...AbdullaAlAsif1
The Swinhoe's softshell turtle, Rafetus swinhoei (Gray, 1873),) is one of the world's largest freshwater turtles, and possibly the most endangered turtle species on the planet (Stanford et al., 2018). It has an overall length of over 100 cm and a width of up to 70 cm, and it can easily weigh up to 70–100 kg, maximum weight was recorded at 169 kg (Solimine, 2013; Trong, 2018). Despite its enormous size and unusual look, this species is incredibly secretive and only comes to the surface to breathe, preferring to remain submerged deep down. For this species, there is very little ecological information, and the remaining distribution is unclear. This could explain why it's so difficult to positively identify and confirm occurrences of this species in the wild (Trong, 2018). If we look back to the history and biogeography of this species, it can be found that the existential records were documented in the historical literature of the Chinese and Vietnamese dynasties. This species was once thought to only live along the Red River in China and Vietnam, as well as the lower Yangtze River floodplain in China, but its current population size is estimated to be just one wild individual of undetermined sex and a solitary captivity male in Suzhou Zoo, China. Although recent thorough searches in Yunnan, China, and Vietnam failed to confirm the presence of more wild specimens, some sightings were reported until around a decade ago (Stanford et al., 2018), giving hope that more individuals may yet exist in Vietnam.
Snapper shrimp is a symbiotic organism usually hidden under the rocks, sponges and pen shells in the seagrass and coral habitats. The relationship study within snapper shrimp and pen shell was conducted from Merambong shoal, one of the biggest seagrass beds in peninsular Malaysia. A total of 40 individual pen shells were collected randomly and four species of pen shells were identified. 40 Anchistus custoides were found inhabiting symbiotically in the mantle cavity of the pen shell as solitary males and females and heterosexual pairs. Pen shell, Pinna bicolour and Atrina vexillum recorded the highest average SH 217.79±53.15 mm, SV 2.62±1.36 dm3 and SH 164.10-224.78 mm with the SV 1.18±0.43 dm3, respectively compared to the other species. The size of Anchistus custoides ranged from 15.00 to 20.00 mm in length and it was determined to be female due to the presence of eggs in the pleopods. The length of the cephalothorax and its length were highly related (rs=0.563, p≤0.01, N=40) and found wider in females. A little difference in size between the left and right chela in males of identical length was noticed, although the left chela is much bigger than the right. The significant relationship (rs=0.450, p≤0.01, N=40) between the pen shell length and shrimp (male-female) length revealed that the size of the shell is important to be hosted the snapper shrimp in the shell cavity.
Diversity of fisheries in Sarawak, Northwest Borneo: Present status and conse...AbdullaAlAsif1
Information on fisheries resources in East Malaysia is scarce and poorly known. The current review aims to compile a checklist of fisheries resources in the Northwest Borneo (Sarawak, Malaysia) from available published literature, address the resources' present status, and suggest future monitoring needs for selected critical species. The study also aims to determine several Sarawak land-use issues that are in flux and responsible for habitat degradation. Five hundred sixty-four species belonging to 123 families and 32 orders were recorded from Sarawak waters. Freshwater fish species comprised 48.0% of the total, followed by a marine (36.6%), marine-euryhaline (12.9%), and brackish water (2.5%) species. Of this, Cyprinidae was the most dominant group accounting for the greatest number of species (82 species), followed by Balitoridae (34 species), Bagridae (21 species) and Penaeidae (21 species). Therefore, available fisheries resources should be managed carefully as 48 species (9.0%) are currently vulnerable to extinction. Furthermore, the presence of 20 alien species in Sarawak water bodies also requires attention from the authorities due to the potential disruption of aquatic ecological balance. Changing land use issues in Sarawak such as forest degradation, agricultural expansion, peatland deforestation and conversion, logging, destruction of mangrove forests, and construction of hydroelectric power dams and flood mitigation channels pose significant challenges to fishery management in Sarawak. Our study documents the priority of fishery monitoring and conservation in Sarawak water bodies to ensure sustainable management of fisheries resources.
Availability and potentiality of small indigenous species of fish throughout ...AbdullaAlAsif1
This study was conducted with aiming to know the present status of small indigenous fish species (SIS) in Oxbow Lakes/baors and rivers as well as the availability of SIS of fish and their potentiality in the study area. Regular data were collected from some important fish landing center cum markets, Oxbow Lakes and rivers of the study area. The availability of small indigenous species (SIS) of fish declined to a great extent over the years and many of them are rare or endangered due to death of rivers and baors were brought under aquaculture. Besides these, Many of SIS of fish were being increasingly used as trash fish for fish feed purpose. As an over populated country, demand of SIS of fish is increasing day by day. After 1990s almost all Oxbow Lakes have been gradually trying to bring under aquaculture through local community or Department of Fisheries. The most significant message of this study is that, some vulnerable and endangered SIS of fish are locally abundant both in fish landing center, baors and rivers. So they must be conserved for their bioavailability and nutrition throughout the country that would be most intelligent efforts in favor of global ichthyo-biodiversity conservation.
Socio-economic conditions of the fish farmers in Jessore, BangladeshAbdullaAlAsif1
The study was conducted the fish farmers in some selected area at sadar upazila in Jessore under the district of Jessore from September to December 2013. A total of 100 fishermen were selected by stratified random sampling method and interviewed. The average me mbers of single family were 5-6 people per household. Single families were much more (62 %) than joint families. Most of the fish far mers were Muslims 92% and few of them were Hindus 8%Most of them had the secondary educational background and some of them with different levels of higher education. It was found that the number of school going children was 2-3 per house. Full katcha (17 %) houses were few, while the semi-pucca (40%) and pucca (43%) houses were more abundant. Regarding health and sanitation, 31% fishermen reported to suffer fro m gastric and 17% suffered from fever. Most of the interviewees were found to take credit facilities from different sources for the subsistence of their family and their venture. The 98% were found to have their tube-wells, others using Governmental tube-well, or those belonging to schools or neighbors However, they need more institutional, organizational, and technical and credit support for their better socioeconomic and sustainable livelihood.
Socio-economic condition of fish fry and fingerling traders in greater Jessor...AbdullaAlAsif1
The present study was conducted to assess the livelihood status of fish fry and fingerling traders and socioeconomic condition in the greater Jessore, Bangladesh from April 2013 to September 2013. Data were collected through the use of well-structured questionnaire from the selected area. 73% of traders have single family but only 27% have joint family. 45% fish fry and fingerling traders family has four members. 20% family has five members, 14% family has six members, 12% family has three members 9%family has seven to eight members respectively. 92% traders has own bank account but only 7% traders uses their bank account when they trade fry and fingerling. 87% traders have secondary business like rickshaw business, fruit business, cloth business, fish trading etc. Among 300 fish fry and fingerling traders the sanitation facilities are present 100%. It was found that 69% and 31% of fish farmers used semi-pucca and pucca toilet respectively. Among 300 fish fry and fingerling traders 78% of traders have own house but only 22% of trader live in rental house or place. 46% of traders have institutional education that range from primary to higher education and other 54% of traders have no experience in education. In case of illness 58% of traders and trader's family goes to government hospital, 24% uses private clinic, 15% traders are uses local village doctor and another 3% does not get any treatment or not require no treatment. 93% traders have television, 5% house consist radio and another 2% house no instrument for entertainment. Only 10% trader wants their son and grandson engages with this fish fry and fingerling trading profession. The average monthly income of traders in the peak season is 18000 taka and off-peak season the income is less than 7000 taka per month.
Marketing of fish and fishery products in Dinajpur and livelihoods of the fis...AbdullaAlAsif1
Marketing of fish and fishery products in Dinajpur and livelihoods of the fish retailers were studied during the period from January to May, 2013. A total of 60 fish retailers were interviewed for obtaining pertinent data. A survey questionnaire was developed, pre-tested and finally used for collection of data. The collected data were tabulated using conventional statistical tools. The result showed that the livelihoods of a large number of people are associated with fish and fishery products distribution and marketing in six different markets. Three types of marketing channel were found to be operated in the selected markets. The shorter marketing chain which included the fish farmers, retailers and consumers was found to be more beneficial to the fish producers. Fish traders in village markets generally operate a capital of around TK. 8,000-10,000 and traders in town markets a capital of around TK. 10,000-20,000 per day. It was found that most of the fish traders used their own money. Price of fish depends on market structure, species, and freshness, supply demand of fish and size of fishes. There are seasonal variation in prices with the highest in summer (March to May), and lowest in pre-winter: (September to November) during the fish harvesting season. Different types of dried and salted fish were sold in the markets. Traders have broadly improved their food consumption facilities, standard of living, and purchasing power as an economic sector. However, the unhygienic conditions of the marketplace, lack of ice facilities, poor infrastructure, inadequate storage and poor transportation facilities were reported to be the major constraints hindering the marketing system in the surveyed areas. It is therefore necessary to provision of governmental, institutional and banking assistance for improvement of the marketing system.
Socio economic status of fry collectors at Sundarban regionAbdullaAlAsif1
The study was conducted on the livelihood approach of fry collectors at Shyamnagar upazila under Satkhira district. The objective of the study was to assess the present livelihood status, to identify the socio-economic condition and problem associated with the alternative livelihood approach of fry collectors. The survey was conducted on three unions of Shyamnagar upazila adjacent to Sundarban as study area. In the study area average family size was 5 persons per family. The maximum age level was 20-30 years old. Among the fry collectors 66% was male and 34% was female. Among them 62% was Muslim and 38% was Hindu. About 78% of total fry collectors are landless in the study area. Literacy was 31% of them where female literacy was 28% and male literacy was 72% and most of them can sign only. Most of the collectors about 63% lived in mud walled with golpata shed house. About 73% families have two earner and 68% have single family. Peak season of fry collection was March to May. For collecting fry they use push net, set bag net mainly. They collect fry in the early morning and in early evening for about 6-8 hours. Men collect 150-200 pieces of fry per day in peak season and 40-60 pieces of fry by women. Price varies from 800-3500 Tk. per thousand golda fry. Price is higher in the month on March-May. About 52% collectors engaged in fishing during off season as alternative income source. Their preferable alternative income source are sustainable agriculture, fry nursing, poultry farming, planned fish cultivation, cattle and goat rearing etc. The main problem of converting of fry collection to other profession was lack of other training of other works, lack of capital, poor infrastructure.facilities, absence of industrialization, lack of land, government and NGO level work is very limited.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
@kuddlelifefoundation
Our Linkedin Page:
https://www.linkedin.com/company/kuddlelifefoundation/
and write to us if you have any questions:
info@kuddlelife.org
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
Follow us on: Pinterest
Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Micro RNA genes and their likely influence in rice (Oryza sativa L.) dynamic ...Open Access Research Paper
Micro RNAs (miRNAs) are small non-coding RNAs molecules having approximately 18-25 nucleotides, they are present in both plants and animals genomes. MiRNAs have diverse spatial expression patterns and regulate various developmental metabolisms, stress responses and other physiological processes. The dynamic gene expression playing major roles in phenotypic differences in organisms are believed to be controlled by miRNAs. Mutations in regions of regulatory factors, such as miRNA genes or transcription factors (TF) necessitated by dynamic environmental factors or pathogen infections, have tremendous effects on structure and expression of genes. The resultant novel gene products presents potential explanations for constant evolving desirable traits that have long been bred using conventional means, biotechnology or genetic engineering. Rice grain quality, yield, disease tolerance, climate-resilience and palatability properties are not exceptional to miRN Asmutations effects. There are new insights courtesy of high-throughput sequencing and improved proteomic techniques that organisms’ complexity and adaptations are highly contributed by miRNAs containing regulatory networks. This article aims to expound on how rice miRNAs could be driving evolution of traits and highlight the latest miRNA research progress. Moreover, the review accentuates miRNAs grey areas to be addressed and gives recommendations for further studies.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
UNDERSTANDING WHAT GREEN WASHING IS!.pdfJulietMogola
Many companies today use green washing to lure the public into thinking they are conserving the environment but in real sense they are doing more harm. There have been such several cases from very big companies here in Kenya and also globally. This ranges from various sectors from manufacturing and goes to consumer products. Educating people on greenwashing will enable people to make better choices based on their analysis and not on what they see on marketing sites.
Diabetes is a rapidly and serious health problem in Pakistan. This chronic condition is associated with serious long-term complications, including higher risk of heart disease and stroke. Aggressive treatment of hypertension and hyperlipideamia can result in a substantial reduction in cardiovascular events in patients with diabetes 1. Consequently pharmacist-led diabetes cardiovascular risk (DCVR) clinics have been established in both primary and secondary care sites in NHS Lothian during the past five years. An audit of the pharmaceutical care delivery at the clinics was conducted in order to evaluate practice and to standardize the pharmacists’ documentation of outcomes. Pharmaceutical care issues (PCI) and patient details were collected both prospectively and retrospectively from three DCVR clinics. The PCI`s were categorized according to a triangularised system consisting of multiple categories. These were ‘checks’, ‘changes’ (‘change in drug therapy process’ and ‘change in drug therapy’), ‘drug therapy problems’ and ‘quality assurance descriptors’ (‘timer perspective’ and ‘degree of change’). A verified medication assessment tool (MAT) for patients with chronic cardiovascular disease was applied to the patients from one of the clinics. The tool was used to quantify PCI`s and pharmacist actions that were centered on implementing or enforcing clinical guideline standards. A database was developed to be used as an assessment tool and to standardize the documentation of achievement of outcomes. Feedback on the audit of the pharmaceutical care delivery and the database was received from the DCVR clinic pharmacist at a focus group meeting.
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
An investigation on heavy metal tolerance properties of bacteria isolated from textile effluent
1. J. Bio. & Env. Sci. 2015
62 | Ashikuzzaman et al.
RESEARCH PAPER OPEN ACCESS
An investigation on heavy metal tolerance properties of
bacteria isolated from textile effluent
Md. Ashikuzzaman1
, Sayeed Shahriyar2*
, Mohammed Bakhtiar Lijon3
, Md. Atiqur
Rahman4
, Md. Mahedi Hassan5
, Abdulla-Al-Asif6
1
Department of Microbiology, Jessore University of Science and Technology, Bangladesh
2
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh Bangladesh
3
Modern Food Testing Laboratory, Chittagong City Corporation, Bangladesh
4
Department of Environmental Science, Bangladesh Agricultural University, Mymensingh,
Bangladesh
5
The ACME Laboratories Ltd. Dhaka, Bangladesh
6
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh
Article published on December 12, 2015
Key words: Heavy metal, Textile effluent, Bacteria.
Abstract
The presence of high concentration of toxic heavy metals in industrial waste directly leads to contamination of receiving soil and
water bodies and has deleterious impact on both human health and aquatic life. In the present study samples from textile mill
effluent from different areas of Jessore city were analyzed for the identification and characterization of bacteria which shows
tolerance to Copper, Mercury and Zinc. The bacterial isolates were characterized on the basis of their morphological and
physiological studies including size and shape of the organisms, arrangement of the cells, presence or absence of spores, regular
or irregular forms, gram reaction, cultural characteristics, IMViC test, H2S production, nitrate reduction, deep glucose agar test
etc. All the bacterial isolates belonged to 3 genera Bacillus, Enterobacter and Pseudomonas. All the gram positive isolates used
in our study showed highest level of tolerance to Zn and moderate level of tolerance to Cu while gram negative isolates showed
higher tolerance to Zn in comparison with Cu in nutrient broth. But all of the isolates showed almost no tolerance to Hg. So, our
bacterial isolates have the probability to use in the treatment of industrial effluent containing heavy metals and thus pollution
due to heavy metal can be controlled. The goal of this study was to identify heavy metal tolerant bacteria from the textile
effluent. This kind of study is very significant for broader investigation to obtain data about metal tolerant bacteria considering
their potential use for bioremediation and about the interactions between metals and bacteria.
*Corresponding Author: Sayeed Shahriyar m15121415@bau.edu.bd
Journal of Biodiversity and Environmental Sciences (JBES)
ISSN: 2220-6663 (Print) 2222-3045 (Online)
Vol. 7, No. 6, p. 62-71, 2015
http://www.innspub.net
2. J. Bio. & Env. Sci. 2015
63 | Ashikuzzaman et al.
Introduction
Various manufacturing processes are carried out for
different types of textiles. This process uses a large
amount of water. Finishing is the final step in
manufacturing and uses chemicals like HS-
ULTRAPHIL, ECODESIZEPS- 10 and Amino silicone
fluid to treat the cloths for obtaining a better quality
(Wang et al., 2002). So the different manufacturing
steps, such as mercerization, bleaching,
neutralization, dyeing, printing and finishing in
Textile industry consumes huge amount of dyes,
chemicals and water and also produces large volumes
of textile wastewater effluents containing heavy
metals Chromium (Cr), Lead (Pb), Copper (Cu),
Mercury (Hg) and Zinc (Zn), dyes, organic and
inorganic acids and salts, bleaching agent etc., in
variable concentration. These untreated industrial
effluents not only deteriorate surface water quality,
ground water, soil and vegetation, but also cause
many diseases like haemorrhage, ulceration of skin,
nausea, severe irritation of skin and dermatitis (Nese
et al., 2007). The term heavy metal applies to the
group of metals and metalloids with atomic density
greater than 4000 kg m-3, or 5 times more than water
(Garbarino et al., 1995). In nature, there are about 50
heavy metals of special concern because of their
toxicological effect to human beings and other living
organisms although some are necessary for living
organisms at certain concentration levels. Heavy
metals cannot be degraded or destroyed because they
are stable and so persistent environmental
contaminants. Bacteria that demonstrate the capacity
of surviving in toxic heavy metal concentrations have
been isolated from different sources (Basu et al.,
1997; Castro-Silva, 2003; Choudhury and Kumar,
1998; Duxbury, 1986; Haefeli et al., 1984; Lima-
Bittencourt et al., 2007; Otth et al., 2005). Many
bacteria have specific genetic mechanisms of
resistance to toxic metals (Mindlin et al., 2001; Silver
and Misra, 1988). Aquatic microbes become resistant
to antibiotics and metals as a result of contamination
with effluents. The term tolerance seems more
appropriate to refer to the ability of a bacterial strain
to grow in the presence of high concentration of a
metal. The presence of those elements in the
environment can result in impacts on ecosystems,
with alterations in the biomass, diversity of microbial
communities and cycling of elements (Roane, 1999;
Sobolev, 2008). Microbiology and biotechnological
approaches have been used for the removal of
metallic ions from industrial waste water appear to
present a low cost application, not an additional
factor of environmental pollution and allows recovery
of heavy metals from industrial waste waters.
Advantages of microbiological method for removing
heavy metals led to increased laboratory studies to
improve the removal efficiency of metals from
industrial waste waters. Despite the large number of
papers describing the action of heavy metals on
microorganisms, there are few studies on the effects
of toxic metals in the physiology of metal tolerant
bacteria, in comparison to those about their
inhibitory or deleterious effects on susceptible
organisms (Lima et al., 2012). So the major goal of
this study was identification and characterization of
bacteria isolated from the sample of textile effluents
possessing tolerance to heavy metals like Cu, Hg and
Zn.
Materials and methods
Collection and Preservation of samples
The sample for the research work was taken from
textile mill effluent from different sites of Khayertala
of Jessore city. Different types of samples were
collected in labeled bottles aseptically. After
collection, the samples were preserved in the
refrigerator.
Isolation, purification and preservation of
microorganisms
The success of biotechnological process chiefly
depends on the used strain of microorganism. For
this reason, the first step is the isolation of concerned
microbes from their natural habitats.
a. Media Used for Isolation: For the isolation of
microbes nutrient agar media was used.
3. J. Bio. & Env. Sci. 2015
64 | Ashikuzzaman et al.
b. Isolation Procedures: Pour plate technique
was applied for enumeration and isolation of bacteria
through serial dilution was carried out (Greenberg et
al., 1980). One ml of the suspension of effluent was
transferred to 9 ml of sterile distilled water for tenfold
(1:10) dilution and further diluted up to 106 dilutions.
Then the media were incubated at 37°C for 24 to 48
hours.
c. Purification: The isolated organisms were
then purified through both pour plate and streak
plate methods repeatedly on nutrient agar media.
d. Preservation and Maintenance: The purified
bacterial and fungal isolates were then transferred to
nutrient agar slant in two sets of culture tubes to
reduce the possibility of contamination. One set of
culture tubes was preserved as stock culture. The
second set was used for laboratory work.
Identification of selected isolates
For the identification of selected isolates the following
morphological characteristics and physiological
behaviors were observed.
A. Morphological studies of the selected
isolates
Morphological characters such as size, shape,
arrangement, color etc., of selected isolates were
observed by microscopic methods.
With a view to identify the selected strains the
following morphological characters were studied:
i. Cultural characteristics
Agar colony
Morphological characters such as size, shape,
elevation, opacity, surface and color of the colony
were studied.
Agar slant
The modes of bacterial growth on slants were studied.
Broth culture
Production of turbidity, sedimentation and surface
growth in nutrient broth was observed and noted.
ii. Microscopic characteristics
For the study of size, shapes and sporulation of the
vegetative cells microscopic method was used. The
length and breadth of cells and spores were
measured.
Preparation for microscopic examination
Fixed Stained Smears
The Techniques used to obtain information on shape,
anatomy, and taxonomic features of the cells that
cannot be easily seen in unstained materials.
Simple staining
For this purpose 5% aqueous solution of basic stains
such as methylene blue, crystal violet,
mercurochrome, safranin etc. were used. The slides
were examined under microscope.
Gram staining
The fixed smear was flooded with ammonium oxalate
crystal violet solution for 3 minutes. This was gently
rinsed off and an iodine solution was applied for 30
seconds. Followed by gentle washing with water ethyl
alcohol (95%) was then applied for 20 seconds to
decolorize the strain. Finally safranine was used as a
counter stain for 3 minutes. Then the slide was gently
rinsed off with water and blotted dry. The result was
recorded as gram positive and gram negative.
B. Physiological studies of the selected isolates
Physiological characteristics are more important than
morphological characteristics in the identification of
bacteria. For this reason, the following studies were
done on the physiological activities of the selected
bacterial isolates.
i) Catalase test
To observe the activity, nutrient broth tubes were
inoculated with 48 to 72 hours old culture and
incubated at 3720C for 48 hours. After incubation
then observation was made closely for the appearance
of oxygen bubbles. Production of bubbles indicates
4. J. Bio. & Env. Sci. 2015
65 | Ashikuzzaman et al.
the positive result Fig. 1.
ii) Glucose broth
To observe the growth of organism in liquid culture,
glucose broth medium was prepared and inoculated
with selected strains. After incubation the appearance
of growth were recorded.
iii) Indole test
Indole is nitrogenous compound and a degradation
product of amino acid tryptophane by various
bacteria. Indole can be detected by the following two
methods:
(a) Tryptophane broth tubes were inoculated with 48
to 72 hours nutrient broth cultures. A filter paper
strip, which was soaked in a saturated solution of
oxalic acid, then dried and kept in the tube by holding
them between the cotton plug and the wall of the test
tube. After over right incubation the paper becomes
pink. This indicates the formation of indole.
(b) Kovac’s Method
Tryptophane broth tubes were inoculated with the 48
to 72 hours nutrient broth cultures. After incubation
at 3720C for 24 to 48 hours, few drops of kovac’s
reagent was added to it, shaken and allowed the
reagent to float. Indole formation is detected by the
appearance of a pink colour at the top. Controlled
tube will produce bottle green ring at the top of the
medium.
iv) Test for nitrate
Test reduction of nitrate is brought about by several
bacteria. The end products of nitrate reduction are
nitrite ammonia, nitrous oxide, nitrogen gas etc. The
organisms contain the enzyme nitrate reductase is
able to reduce the nitrate to nitrite.
The following reagents were required for this test
Reagent A
Sulfanilic acid was dissolved in one liter of 5N acetic
acid and stored in brown glass bottle.
Reagent B
6.0 ml of dimethyl napthalamine was added to one
liter of 5N acetic acid and stored in brown bottle.
Reagent C
Small amount of zinc dust.
After incubation a few drops of solution A and equal
volume of solution B was added and shaken well.
Formation of red to pink color indicates the reduction
of nitrate to nitrite. A small piece of Zn dust was
added to the tubes, where solution A and B was
already added. Any remaining nitrate (in case) would
be reduced to nitrite resulting red to pink color.
v) Citrate utilization
The ability to utilize citrate as sole source of carbon
and energy can be used to distinguish certain gram
negative rods. For this test citrate medium was
inoculated with at 3720C for 48 hours. Appearance
of turbidity or growth in the medium indicated the
utilization of citrate.
vi) Production of hydrogen sulphide
Peptone iron agar medium was inoculated and lead
acetate paper was introduced in each of the tubes
containing peptone iron agar medium by holding
them between the plug and the wall of the test tube.
After incubation the production of hydrogen sulphide
was indicated by blackening of the lead acetate paper.
vii) Starch hydrolysis
After the microbial growth iodine solution was added
to each of the starch agar plates. Development of clear
white to bluish brown color indicated the complete
and partial hydrolysis of the starch respectively,
hence the presence of enzyme amylase. Development
of deep blue color indicated that the starch had not
been hydrolyzed.
viii) Voges-Proskaur (V.P.) test
The acidic product produced by some bacteria
converted to metabolic intermediate, pyruvic acid and
then to neutral products and CO2. Voges-Proskaur
medium was inoculated and incubated. After the
5. J. Bio. & Env. Sci. 2015
66 | Ashikuzzaman et al.
growth 3 ml napthol solution was added to each of the
test tubes followed by one ml of potassium hydroxide-
creatine solution. The tubes were then shaken
vigorously for 1-2 minutes. Appearance of a crimson
ruby color in the tube indicated the positive result.
ix) Methyl red reaction
After incubation of the prepared V.P. medium a few
drops of methyl red solution were added in each tube.
A distinct red color indicated methyl red positive and
yellow color indicated methyl red negative.
x) Casein hydrolysis
Nutrient skimmed milk medium was prepared by
mixing sterilized skimmed milk (1-2 ml) and sterilized
nutrient agar (10-15 ml) in petri plates. After
inoculation and incubation, the plates were observed
as the organisms produced caseinase were
surrounded by clear zone of hydrolysis of casein. But
the controlled plate was found opaque due to the
presence of casein Fig. 2.
xi) Motility test
Deep tube of nutrient agar medium was prepared
with indicator terazolium chloride and sterilized. At
450C the tubes were inoculated by the selected
isolated microorganisms and incubated for 2 to 5
days. The organisms which were motile changed the
color of the medium form colorless to pink to red.
Evaluation of metal tolerance
The bacterial isolates were tested for their tolerance
to Cu, Hg and Zn by broth dilution method. For this
test, different concentration of heavy metal
containing nutrient broth media was prepared and
dispensed into the test tubes (10 ml/tubes) and
sterilized in an autoclave. These modified broths were
inoculated with the equal amounts of individual
selected organism isolated from the industrial
effluent by preparing suspension. For comparing the
growth response at different ppm, one set of nutrient
broth (without the heavy metal) containing test tubes
were inoculated with the selected organisms and used
as control. Then incubated at 37°C for 2 to 4 days and
observed periodically.
Results
During the period of study, a total number of 10
bacterial colonies were isolated. Out of these isolates,
5 isolates were selected for further study on the basis
of their morphological and cultural characteristics
and better growth on nutrient agar media. The
selected isolates were designated as NS1, NS2, NS3,
ES1 and CS1.
On nutrient agar plate bacterial isolates showed
different type of colonies. They were differed from
each other in color, form, margin and elevation.
Bacterial isolates were characterized on the basis of
their morphological characteristics including size and
shape of the organisms, arrangement of the cells,
presence or absence of spores, regular or irregular
forms, gram reaction, cultural characteristics, IMViC
test, H2S production, nitrate reduction, deep glucose
agar test etc.,(Table.1). All these characteristics were
then compared with the standard description of
“Bergey’s Manual of Determinative Bacteriology”
8thed. (Buchanan and Gibbons, 1974). All the isolates
were found to belong to 3 genera, such as, Bacillus,
Enterobacter and Pseudomonas. An attempt was
made to identify them up to species and provisionally
identified as Bacillus circulans (NS3), Bacillus
pulvifaciens (NS2), Bacillus sphaericus (NS1),
Enterobacter cloacae (ES1) and Pseudomonas putida
(CS1).
All the three gram positive bacteria NS1, NS2 and
NS3 shown similar kind of characteristics specially
spore formation and turbid and sediment formation
in both Nutrient and Glucose broth. On the other
hand positive gram negative bacteria ES1 and
CS1shown similar characteristics especially no spore
formation. But differences were noticed for belonging
to different genus. Microbial tolerance to Copper
(Cu), Mercury (Hg) and Zinc (Zn) was studied
visually. Selected five isolates were allowed to grow in
nutrient broth modified with Cu, Hg and Zn
concentrations range from 10 ppm to 150 ppm.
6. J. Bio. & Env. Sci. 2015
67 | Ashikuzzaman et al.
Table 1. Morphological, cultural and biochemical characteristics of different isolates.
ES1 CS1 NS1 NS2 NS3
Vegetative cell Short rod Short rod Rod Rod Rod
Cell arrangement Single Single Single or in pair Single or in pair Single or in
pair
Gram staining Gram Negative Gram Negative Gram positive Gram Positive Gram Positive
Spore staining Non spore
former
Non spore former Spore former Spore former Spore former
Nutrient broth Turbid,
Sediment
Sediment Turbid, Sediment Turbid,
Sediment
Turbid,
Sediment
Glucose broth Turbid,
Sediment
Turbid, Ring Turbid, Sediment Turbid,
Sediment
Turbid,
Sediment
Motility test - - - - -
Indole test - - - - -
MR test - - - - -
VP test + - - + -
Catalase test Slightly + + + + +
Citrate test + + + + +
Nitrate reduction
test
- - - - -
H2S production - - - - -
Casein hydrolysis + + + + +
Starch hydrolysis - - - - -
Enterobacter
cloacae
Pseudomonas
putida
Bacillus sphaericus Bacillus
pulvifaciens
Bacillus
circulans
+: Positive reaction, -: Negative reaction.
In the present study only the NS1 isolates were able to
grow with copper (CuSO4. 5H2O) upto the
concentration of 130 ppm. The gram positive bacteria
have shown more tolerance than the gram negatives
(Table 2).
Table 2. Copper (CuSO4. 5H2O) tolerance of selected isolates.
Isolate
No.
Growth activity
(turbidity) in nutrient broth
Tolerance activity at various ppm
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150
ES1 +++ +++ +++ ++ ++ ++ + + + + - - - - - -
CS1 +++ +++ +++ +++ +++ ++ ++ ++ ++ + + - - - - -
NS1 +++ +++ +++ +++ +++ +++ +++ +++ +++ ++ ++ + + + - -
NS2 +++ +++ +++ +++ +++ +++ ++ ++ ++ ++ + + - - - -
NS3 +++ +++ +++ +++ +++ +++ +++ +++ ++ ++ ++ + + - - -
Note: + = Positive (+= scanty, ++=moderate, +++= heavy), - = Negative.
All the isolates were sensitive to mercury being able to
tolerate only 10 ppm mercury (HgCl2). This states
that the textile effluent discharges no or very small
amount of Hg (Table 3).
Table 3. Mercury (HgCl2) tolerance of selected isolates.
Isolate No. Growth activity
(turbidity) in nutrient broth
Tolerance activity at various ppm
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150
ES1 +++ + - - - - - - - - - - - - - -
CS1 +++ + - - - - - - - - - - - - - -
NS1 +++ + - - - - - - - - - - - - - -
NS2 +++ + - - - - - - - - - - - - - -
NS3 +++ + - - - - - - - - - - - - - -
Note: + = Positive (+= scanty, ++=moderate, +++= heavy), - = Negative.
7. J. Bio. & Env. Sci. 2015
68 | Ashikuzzaman et al.
All the isolates of this study were more tolerant to
zinc (ZnCl2) than copper and mercury. The result also
indicates the higher Zn tolerance of gram positives
than gram negatives. The highest (140 ppm) tolerance
was observed in NS1 while the isolate ES1 was able to
grow up to the concentration of 110 ppm Zn. It result
also tells that the textile effluent releases Zn at very
high concentration (Table 4).
Table 4. Zinc (ZnCl2) tolerance of selected isolates.
Isolate
No.
Growth activity
(turbidity) in nutrient
broth
Tolerance activity at various ppm
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150
ES1 +++ +++ +++ +++ +++ +++ ++ ++ ++ + + + - - - -
CS1 +++ +++ +++ +++ +++ +++ +++ +++ +++ ++ ++ ++ + + - -
NS1 +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ ++ ++ + + -
NS2 +++ +++ +++ +++ +++ +++ +++ ++ +++ ++ ++ ++ + + - -
NS3 +++ +++ +++ +++ +++ +++ +++ ++ ++ ++ + + + - - -
Note: + = Positive (+ = scanty, ++ = moderate, +++ = heavy), - = Negative.
Discussion
Industrial effluents discharged into river or in land
without any treatment can cause severe pollution with
carcinogenic substances. Microbes have wide spread
capacity to remove, transform and precipitate these
chemical pollutants from the surroundings (Faisal
and Hasnain, 2004; Kumar et al. 2005; Raghukumar
et al. 2006).
Fig. 1. Catalase Test: Production of oxygen bubbles
by Bacillus, Enterobacter and Pseudomonas.
In the present study, five bacterial species were
isolated from the textile mill effluent belonging to 3
genera, such as, Bacillus, Enterobacter and
Pseudomonas. The isolates were putatively
indentified as Bacillus circulans, Bacillus
pulvifaciens, Bacillus sphaericus, Enterobacter
cloacae and Pseudomonas putida on the basis of
their cultural, morphological and biochemical
characteristics. Their heavy metal tolerance was
studied by growing them in nutrient broth modified
with Cu, Hg and Zn concentrations ranging from 10
ppm to 150 ppm. Similar findings of occurrence of
heavy metal resistant bacteria in contaminated soil
samples have been reported (Olukoya et al., 1997;
Fagade and Adetutu, 1999; Oyetibo et al., 2010;).
Fig. 2. Casein hydrolysis by Enterobacter cloacae.
The study says that Bacillus sphaericus and Bacillus
circulans shows highest tolerance to Cu of 130 ppm
and 120ppm respectively in comparison with the rests
showing tolerance to 100 ppm. This result states that
the textile effluent discharges considerable amout of
8. J. Bio. & Env. Sci. 2015
69 | Ashikuzzaman et al.
Co. In case Hg, all the isolates were sensitive to
mercury being able to tolerate only 10 ppm whereas
De et al., (2003) isolated two strains from an area
with intense shipping traffic, which grew on seawater
nutrient agar solid medium with 75 ppm mercury.
According to another study of Durve et al., (2012) the
actual heavy metal concentration was calculated and
it was seen that Pseudomonas aeruginosa could
tolerate 294.60 ppm of Mercury resulting that the
effluent of the textile do not release considerable
amount of Hg.
The study shows that all the isolates were more
tolerant to Zn than Cu and Hg where both
Pseudomonas putida and Bacillus pulvifaciens were
moderately tolerant to 110 ppm and able to grow up
to the concentration of 110 ppm on the other hand
Enterobacter cloacae could tolerate up to 140 ppm
concentration of Zn. The result states that the effluent
releases Zn more than Cu and Hg and in considerable
amount.
Presence of metal tolerant bacteria in a given
environment may be an indication that such area is
affected by heavy metals. Isolation of bacteria from
metal polluted environment would represent an
appropriate practice to select metal
resistant strains that could be used for heavy metal
removal and bioremediation purposes (Malik, 2004).
Microbial metal bioremediation is an efficient
strategy due to its low cost, high efficiency and eco-
friendly nature. Microbiological detoxification of
polluted water is economical, safe and sustainable
(Eccles, 1995).
Under conditions of high levels of heavy metals in
their environment, metal resistance in bacteria most
likely help them to adapt faster by the spread of
resistant factors. The organisms with heavy-metal
resistance isolated and identified in this study have
potential application in bioremediation of
environments polluted with metals and may also help
to overcome the inhibition that heavy metals exert on
the biodegradation of organic pollutants. The studies
assessing the potential ability of the selected isolates
to remove heavy metals from contaminated industrial
waste water and their plasmid profiles are currently
underway.
Conclusion
The present study demonstrates that all the isolates
used in our study showed high level of resistance to
zinc and moderate level of resistance to copper and
mercury in nutrient broth. More investigation can be
done to obtain data about metal tolerant bacteria
considering their potential use for bioremediation, as
well as about the impact resulting from the
interactions between metals and metal tolerant
bacteria. Pollution by heavy metal can be prevented
or reduced in a large through the implication of
biotechnology in huge textile effluent management.
Acknowledgement
The authors are grateful to the Department of
Microbiology, Jessore University of Science and
Technology, Jessore-7408, Bangladesh.
References
Basu M, Bhattacharya S, Paul AK. 1997.
Isolation and characterization of chromium-resistant
bacteria from tannery effluents. Bulletin of
Environmental Contamination and Toxicology 58,
535-542.
Castro-Silva MA, Souza Lima, AO, Gerchenski
AV, Jaques DB, Rodrigues AL, Lima de Souza
P, Rörig LR. 2003. Heavy metal resistance of
microorganisms isolated from coal mining
environments of Santa Catarina. Brazillian Journal of
Microbiology 34, 45-47.
Choudhury P, Kumar R. 1998. Multidrug and
metal-resistant strains of Klebsiellapneumoniae
isolated from Penaeusmonodon of the coastal waters
of deltaic Sundarban. Canadian Journal of
Microbiology 44, 186-189.
De J, Ramaiah N, Mesquita A, Verlekar XN.
9. J. Bio. & Env. Sci. 2015
70 | Ashikuzzaman et al.
2003. Tolerance to various toxicants by marine
bacteria highly resistant to mercury. Marine
Biotechnology 5, 185-193.
Durve A, Naphade S, Bhot M, Varghese J,
Chandra N. 2012. Characterisation of metal and
xenobiotic resistance in bacteria isolated from textile
effluent. Pelagia Research Library Advances in
Applied Science Research 3, 2801-2806.
Duxbury T. 1986. Microbes and heavy metals: an
ecological overview. Microbiology Science 3, 330-333.
Eccles H. 1995. Removal of heavy metals from
effluents streams- Why select a biological process?
International Biodeterioration & Biodegradation 35,
5–16.
Fagade OE, Adetutu EM. 1999. Lead solubilization
and accumulation by two strains of Pseudomonas
species obtained from a battery manufacturing
factory effluent. Nigeria Journal of Miccrobiology 13,
39-46.
Faisal M, Hasnain S. 2004. Microbial conversion
of Cr (VI) in to Cr (III) in industrial effluent. African
Journal Biotechnology 3, 610-617.
Garbarino JR, Hayes H, Roth D. 1995.
Contaminants in the Mississippi river. U. S.
Geological Survey Circular, Virginia, U.S.A. 1133 p.
Haefeli C, Franklin C, Hardy K. 1984. Plasmid-
determined silver resistance in Pseudomonas stutzeri
isolated from a silver mine. Journal of Bacteriology
158: 389-392.
Kumar RA, Moharikar A, Purohit HJ. 2005.
Microbial population dynamics at effluent treatment
plants. Journal of Environmental Monitoring 7, 552-
558.
Lima e Silva AA. 2012. Heavy metal tolerance (Cr,
Ag AND Hg) in bacteria isolated from sewage.
Brazilian Journal of Microbiology: 1620-1631.
Malik A. 2004. Metal bioremediation through
growing cells. Environmental International 30, 261-
278.
Mindlin S, Kholodii G, Gorlenko Z, Minakhina
S, Minakhin L, Kalyaeva E, Kopteva A, Petrova
M, Yurieva O, Nikiforov V. 2001. Mercury
resistance transposons of Gram-negative
environmental bacteria and their classification.
Research in Microbiology 152, 811-822.
Nese T, Sivri N, Toroz I. 2007. Pollutants of
Textile Industry Wastewater an Assessment of its
Discharge Limits by Water Quality Standards.
Turkish Journal of Fisheries and Aquatic Sciences 7,
97-103.
Olukoya DK, Smith SI, Ilori MO. 1997. Isolation
and characterization of heavy metals resistant
bacteria from Lagos Lagoon. Folia Microbiol (Praha).
42, 441-444.
Oyetibo GO, Ilori MO, Adebusoye SA, Obayori
OS, Amund OO. 2010. Bacteria with dual resistance
to elevated concentrations of heavy metals and
antibiotics in Nigeria in contaminated systems.
Environmental Monitoring Assessment 168, 305-314.
Raghukumar C, DeSouza DT, Tiwari R, Sah
AK. 2006. Enhanced production of laccase by a
marine fungus during treatment of coloured effluents
and synthetic dyes. Enzyme and Microbial
Technology 38, 504-511.
Silver S, Misra TK. 1988. Plasmid-mediated heavy
metal resistances. Annual Review of Microbiology 42,
717-743.
Sobolev D, Begonia MF. 2008. Effects of heavy
metal contamination upon soil microbes: lead-
induced changes in general and denitrifying microbial
communities as evidenced by molecular markers.
10. J. Bio. & Env. Sci. 2015
71 | Ashikuzzaman et al.
International Journal of Environmental Research and
Public Health 5, 450-456.
Wang C, Yediler A, Lienert D, Wang Z,
Kettrup A. 2002. Toxicity evaluation of reactive
dyestuffs, auxiliaries and selected effluents in textile
finishing industry to luminescent bacteria Vibrio
fischeri. Chemosphere 46, 339-344.