Gonad of sea urchin is considered as food delicacy in many parts of the world due to high nutrition and mineral contents. Several species of sea urchins are available in Malaysia and reported from different habitat, including seagrass beds, coastal breakwater, coral reefs and other ecologically important habitats. The current study was undertaken to evaluate the proximate and mineral composition of long spined sea urchin (Diadema setosum) roe collected from breakwater of Terengganu, Malaysia in October 2019, November 2019 and January 2020. The samples were analysed for proximate including protein, lipid, moisture, and ash content of sea urchin roe. The macro, micro minerals and heavy metals were also evaluated including Ca, Fe, Zn, Cu, Co, Se, Mg, Ni, Pb, Al, and Cd. The result suggested that, long spined sea urchin (Diadema setosum) roe can be considered as good sources of food due to high percentage of protein (ranged between 36.21±0.44 and 50.14±4.63). The presence of heavy metal such as Ni, Pb, and Cd suggesting the breakwater environments were not good enough and the possible sources of heavy metals contamination in sea water environment must be stopped and eradicated. This study provides important information with regard to the proximate and mineral values of D. setosum, as well as the need for a better management of its habitat before D. setosum can be widely promoted as delicacy in this region.
Proximate Analysis of Bait Polychaetes from Port Dickson, Malaysia as Prospec...AI Publications
Bait polychaete worms were obtained from areas around Port Dickson coasts of Negeri Sembilan Darul Khusus in Malaysia. There were four species of bait polychaete species used in this study Perinereis quatrefagesi, Halla parthenopeia, Diopatra neapolitana, and Marphysa mossambica. These polychaete species were subjected to lipid, protein, water, carbohydrate, and ash content analysis. The lipid contents in polychaete were determined using Soxhlet analysis. The protein contents were determined using Kjeldahl analysis. The water and ash content were determined by using the oven drying method. Marphysa mossambica has the highest percentage of lipid as much as 27.98. Diopatra Neapolitana has the highest protein content in their body, as much as 51.87% and followed closer by Marphysa mossambica as much as 51.14% respectively. The highest carbohydrate content was found in the polychaete species Perinereis quatrefagesi, as much as 24.61%. The highest ash content was found in Diopatra neapolitana yet Marphysa mossambbica has comparatively high ash content as well as much as 3.12% compared to 3.24% in Diopatra neapolitana. Similar pattern as in ash content can be seen for water content as well. Diopatra neapolitana has the highest percentage of water as much as 71.38% and followed closer by Marphysa mossambica with water content as much as 70.23%. The biochemical composition in polychaete species varies because of species size, feeding biology, habitat and environmental factor as well. The biochemical composition within species also shows a constant composition even though it varies in terms of value.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
This research was done in Pelabuhan Ratu Bay, about 60 km South Sukabumi Regency. The aim of this study is to determine the suitable area for floating net cage culture that can be developed. The method used in this study is spatial analysis using GIS technique. The data used include secondary and primary data from 2007 until 2008. Spatial analysis result shows, about 8,500 ha of location is suitable for the development of floating net cage culture or about 98% of the total research region worked.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region
Technology of artificial breeding of catfish species in the hatcheries in Jes...AbdullaAlAsif1
The present study was carried out to know the artificial breeding of Ompk pabda, Heteropneustes fossilis and Pangasius hypophthalmus using pituitary gland (PG) from June to August 2014, in the hatcheries in Jessore region. Brood stock of Pangus, Shing, and Pabda was developed in the hatchery. A total of 10 hatcheries were included in the study. Brood fishes were reared in the brood rearing pond by providing artificial diet for good health and full maturation. Brood fishes were injected with PG in different months June to August. Both male and female were injected with PG. In Pabda, Shing and Pangus first PG dose was applied at 8-10, 30-35 and 1-2 mg PG/kg of body weight of females respectively. After 1 st PG dose of Pabda, Shing, and Pangus (6 hours later) female were treated with 2nd doses at 10-12, 60, 8-10 mg PG/kg of body weight, respectively. At the time of second dose of female, the males were also treated with single PG dose. The males of Pabda, Shing and Pangus were treated with 8-10, 30-35 and 1-2 mg PG/kg body weight respectively. It was observed that the female and male brood fishes eject eggs and milt within 12-14 hours respectively. Ovulation occurred in Pabda, Shing, and Pangus naturally after, 6-7 hours. The fertilized eggs of Pabda, Shing and Pangus were hatched within 22-24 hours. The temperature, pH, dissolved oxygen and transparency ranged from 22-34 0 C, 6-8.5, 5-7.5 ppm and 22-32 cm of the water of brood rearing ponds respectively. The average spawn production and body weight of female Pabda, Shing and Pangus were 11500/kg and 120/gm, 21000/kg and 130/gm, 44500/kg and 3.5/kg, respectively. The average fertilization, hatching and survival rate of Pangus (80%, 73% and 64%), Shing (73%, 68% and 58%) and Pabda (78%, 65% and 60%) respectively. The male and female ratio was maintained as 1:2 for each fishes.
Proximate Analysis of Bait Polychaetes from Port Dickson, Malaysia as Prospec...AI Publications
Bait polychaete worms were obtained from areas around Port Dickson coasts of Negeri Sembilan Darul Khusus in Malaysia. There were four species of bait polychaete species used in this study Perinereis quatrefagesi, Halla parthenopeia, Diopatra neapolitana, and Marphysa mossambica. These polychaete species were subjected to lipid, protein, water, carbohydrate, and ash content analysis. The lipid contents in polychaete were determined using Soxhlet analysis. The protein contents were determined using Kjeldahl analysis. The water and ash content were determined by using the oven drying method. Marphysa mossambica has the highest percentage of lipid as much as 27.98. Diopatra Neapolitana has the highest protein content in their body, as much as 51.87% and followed closer by Marphysa mossambica as much as 51.14% respectively. The highest carbohydrate content was found in the polychaete species Perinereis quatrefagesi, as much as 24.61%. The highest ash content was found in Diopatra neapolitana yet Marphysa mossambbica has comparatively high ash content as well as much as 3.12% compared to 3.24% in Diopatra neapolitana. Similar pattern as in ash content can be seen for water content as well. Diopatra neapolitana has the highest percentage of water as much as 71.38% and followed closer by Marphysa mossambica with water content as much as 70.23%. The biochemical composition in polychaete species varies because of species size, feeding biology, habitat and environmental factor as well. The biochemical composition within species also shows a constant composition even though it varies in terms of value.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
This research was done in Pelabuhan Ratu Bay, about 60 km South Sukabumi Regency. The aim of this study is to determine the suitable area for floating net cage culture that can be developed. The method used in this study is spatial analysis using GIS technique. The data used include secondary and primary data from 2007 until 2008. Spatial analysis result shows, about 8,500 ha of location is suitable for the development of floating net cage culture or about 98% of the total research region worked.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region
Technology of artificial breeding of catfish species in the hatcheries in Jes...AbdullaAlAsif1
The present study was carried out to know the artificial breeding of Ompk pabda, Heteropneustes fossilis and Pangasius hypophthalmus using pituitary gland (PG) from June to August 2014, in the hatcheries in Jessore region. Brood stock of Pangus, Shing, and Pabda was developed in the hatchery. A total of 10 hatcheries were included in the study. Brood fishes were reared in the brood rearing pond by providing artificial diet for good health and full maturation. Brood fishes were injected with PG in different months June to August. Both male and female were injected with PG. In Pabda, Shing and Pangus first PG dose was applied at 8-10, 30-35 and 1-2 mg PG/kg of body weight of females respectively. After 1 st PG dose of Pabda, Shing, and Pangus (6 hours later) female were treated with 2nd doses at 10-12, 60, 8-10 mg PG/kg of body weight, respectively. At the time of second dose of female, the males were also treated with single PG dose. The males of Pabda, Shing and Pangus were treated with 8-10, 30-35 and 1-2 mg PG/kg body weight respectively. It was observed that the female and male brood fishes eject eggs and milt within 12-14 hours respectively. Ovulation occurred in Pabda, Shing, and Pangus naturally after, 6-7 hours. The fertilized eggs of Pabda, Shing and Pangus were hatched within 22-24 hours. The temperature, pH, dissolved oxygen and transparency ranged from 22-34 0 C, 6-8.5, 5-7.5 ppm and 22-32 cm of the water of brood rearing ponds respectively. The average spawn production and body weight of female Pabda, Shing and Pangus were 11500/kg and 120/gm, 21000/kg and 130/gm, 44500/kg and 3.5/kg, respectively. The average fertilization, hatching and survival rate of Pangus (80%, 73% and 64%), Shing (73%, 68% and 58%) and Pabda (78%, 65% and 60%) respectively. The male and female ratio was maintained as 1:2 for each fishes.
Productivity of phytoplankton using different organic fertilizers in the glas...AbdullaAlAsif1
The experiment intended to estimate the productivity using different organic fertilizer and to identify the species of phytoplankton in the aquarium tanks. The study was conducted in the laboratory Phytoplankton samples for culture were collected from fisheries experimental pond by using conical-shaped monofilament nylon net (phytoplankton net). Three treatment such as cow dung (T₁), chicken manure (T₂) and control (T₃) were designed. Each treatment had two replications. In case of T₁ and T₂, 10 g of fertilizer was used in each aquarium tank. Sampling of phytoplankton for counting and identification was done every week and 1 litre of water sample was taken from each tank. Phytoplankton sample for identification were preserved by Lugol's solution. Three physicochemical parameters namely water temperature, dissolved oxygen (DO) and pH were measured in every sampling week during the study period. Water quality parameters of the aquarium tank varied with the variation of sampling week. There was no significant difference between water temperature, pH and DO in case of three treatment. The mean water temperature, pH and DO were 21.85±1.95 °C, 8.38±0.23 and 5.55±0.58 mg/l, respectively. The abundance of phytoplankton in T₁, T₂ and T₃ were 25-65, 25-105 and 6-20 individual/l, respectively. The phytoplankton abundance were influenced by different organic fertilizer and noticed higher in number by using chicken manure. Irregular relationship between phytoplankton abundance and water temperature was found. Phytoplankton showed positive relationship with DO. In this study 15 species of phytoplankton were identified under four groups namely Chlorophyta, Cyanophyta, Bacillariophyta and Euglenophyta. Chlorophyta (52%) was the dominant group in the aquarium. The findings of the present study will help to improve the management strategies of water quality, for estimating the productivity of phytoplankton and for the best use of organic fertilizer especially chicken manure.
The effects of different water quality parameters on zooplankton distribution...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online
The Growth of Oreochromis niloticus (2.6g initial average size) was studied in 100m2 earthen ponds for 180 days under tropical field environment. Varying fertilizing regimes consisting of Pennisetum purpureum (PP) and Musa sapientum (MS), two locally available weedy grasses were applied in duplicates in the experimental ponds, at the rate of 0.1-0.2 kg dry matter per m2 per day. Water quality parameters were monitored bimonthly between 8:00am-9:00am . Final average fish weight varied significantly (p<0.05) as follows: 43.85g, 35.5g, 59.5g, 24.9g and 50g, 2 respectively for PP, MS, MS+PP, T0 and T1. Corresponding daily growth were 2.2g/d, 0.1g/d, 0.3g/d, 0.1g/d and 0.2g/d. Survival rate equally varied significantly as follow: 60 %, 67 %, and 98 %, 70 % and 97 % respectively. During the study period, the results indicated the water quality were permissibole limits and can be used for livestock of Oreochromis niloticus .
A potential endemic cyprinid for aquaculture and food security: The embryonic...AbdullaAlAsif1
The Bornean Spotted Barb is endemic fish species and widely distributed throughout Borneo in various freshwater bodies and can be considered as a potential aquaculture candidate for food security and the ornamental trade. However there has yet to be scientific studies on the early life history and developmental stages of this species. Thus the present study reveals the embryonic development of Puntius sealei embryo, from fertilization to post-hatching using the induced breeding method. The induced spawning on P. sealei using Ovaprim hormone at a dose of 0.5 ml/kg for females and 0.25ml/kg for males respectively was successfully conducted and the embryonic development of the fertilized eggs were observed. The zygote, cleavage, blastula, gastrula, segmentation, and larval phases were the six major stages of embryogenesis that investigated in this experiment. This study contributed major knowledge to the early developmental biology of this particular species. The information will be useful for accessing the potentiality of this species for aquaculture as well as the management, and production of the species.
Growth of freshwater mud eel (Monopterus cuchia) in different water condition...AbdullaAlAsif1
An experiment was conducted to study the effect of different feeds on growth and production of freshwater Mud Eel (Monopterus cuchia) in nine aquariums over a period of 90 days during June to August 2016. Three different feeds namely Feed A (live feed), Feed B (supplementary feed) and Feed C (probiotics) were applied to treatments I, II and III respectively. Three types of water used in aquarium. These are pond water, ground water and turbid water. Each of the tanks was stocked with nine fish that's total fish amount of 27. Significantly highest weight gain was obtained in pond water, treatment III, (16.39a ± 2.35). Treatment III yielded the highest (65.56a ±9.4 g/m 2 /year) production with the highest survival rate of fish. Water quality parameters like temperature, dissolved oxygen, P H , and alkalinity were optimum. On the basis of better growth and production, it is suggested that pond water condition with probiotics are suitable for the culture of M. cuchia in aquarium.
Information about composition of deep-sea edible part might raise their value as table food. Proximate composition of few fin fish species from North Atlantic, eastern North Pacific has been reported. No literature is available for either fin fish or shell fish from deep waters off the Indian EEZ.
Evaluation of the oyster farming potential of the Cintra bay (southern Morocco)Origins publication
The bay of Cintra is a marine ecosystem located in the southern Moroccan Atlantic known for its
biological richness and therefore for its high fishery productivity. This bay was chosen as a new destination for the
development of aquaculture in the southern regions of Morocco. To highlight its potential in terms of oyster
farming, a first rearing trial of triploid cupped oysters (Crassostrea gigas) as well as parallel monitoring of the
phytoplankton population and ecological parameters were undertaken. The results obtained showed that the oysters
adapt well to the conditions of the new environment where mortality was negligible, or even absent after a month
of launching. In terms of biological performance, the growth of individuals is continuous during the annual cycle
and after eight months the weight could reach 30.5 g which is a minimum weight for marketing. The AFNOR and
Lawrence and Scott indices showed a good physiological state and a better commercial quality of the oysters. The
filling rate of these oysters, with an average weight of 39.7g when lifting, is very high according to the Lawrence and
Scott index (126.3) and the average AFNOR index (around 21.8) rank them in the “Special” category.
Use of liquid rice starch as a source of carbon for growth of Spirulina plate...AbdullaAlAsif1
A study was conducted to examine the culture and growth performance of S. platensis in three different concentrations (25%, 50% and 100%) of digested liquid rice starch media (DLRSM) with 0.2 g/L urea and Kosaric medium (KM) as control for a period of three months from March to May 2018. Each experiment was done in triplicates for a period of 14 days. The growth rate of S. platensis was found to vary with different concentrations of the medium. The cell weight of S. platensis was attained to highest 12.42 mg/L (dry weight) in KM followed by 11.26 mg/L, 8.35 mg/L and 9.11 mg/L in 25%, 50% and 100% of DLRSM, respectively on the 10 th day of culture period. Similar trend also observed in case of chlorophyll a content of S. platensis. The proximate composition of rice starch was analyzed. The percentage of moisture, protein, lipid, ash, crude fiber and nitrogen free extract (NFE) were95.1, 0.2616, 0.1055, 0.1097, 0.1468 and 3.9669, respectively on the basis of moisture (%) and 4.59, 5.70, 2.30, 2.39, 3.20 and 86.41, respectively on the basis of dry matter (%). The results on the growth performance showed that the growth of S. platensis was significantly (P<0.01) higher when grown in 25% concentration of DLRSM than other concentrations in 50% and 100% of DLRSM. The physico-chemical parameters viz. light intensity (2685 to 2773 lux/m 2 /s), temperature (29.6 to 31.5°C), pH (8.3 to 9.9), alkalinity (1150 to 3032 mg/L), Nitrate-N (1.32 to 6.30 mg/L) and phosphate-P (9.75 to 61.50 mg/L) were within optimum level during the culture period.
ABSTRACT- We conducted a first order analysis on the proximate composition (protein, carbohydrate, fat and astaxanthin) of three dominant seaweed species viz. Enteromorpha intestinalis, Ulva lactuca and Catenella repens inhabiting Indian Sundarbans. The study was conducted at three stations (Gosaba, Bali Island and Jharkhali) during premonsoon, monsoon and postmonsoon of 2014-15. The relevant hydrological parameters (surface water temperature, salinity, pH, dissolved oxygen and dissolved nutrients) were monitored simultaneously during the tenure of the work. ANOVA carried out on the observed data reflects pronounced variations of all hydrological parameters except surface water temperature and salinity between stations. Pronounced seasonal variations were observed for all the selected hydrological parameters. In the domain of proximate composition, ANOVA results exhibit pronounced variations between stations and seasons (except carbohydrate in U. lactuca and C. repens between stations and astaxanthin in U. lactuca between seasons).
Keywords - Seaweed, Indian Sundarbans, Proximate composition, ANOVA, Seasonal variation
Qualitative and quantitative analysis of phytoplankton in culture pond of Noa...AbdullaAlAsif1
A study was conducted on qualitative and quantitative analysis of phytoplankton in cultured ponds of two fish farms of Noakhali district, Bangladesh from 15 September to 15 November 2012 to identify and estimate the abundance of phytoplankton in various culture ponds of two fish farms. Analyses of phytoplankton samples recorded a total of 4 classes phytoplankton viz.; Bacillariophyceae, Chlorophyceae, Cyanophyceae, Euglenophyceae. Out of 21 phytoplankton genera identified, 5 belong to Cyanophyceae, 7 to Chlorophyceae, 5 to Bacillariophyceae and 4 to Euglenophyceae. Among the identified genera, Euglena, Microcystis, Eurolena were found to be dominant genera. Total phytoplankton abundance was varied from 36×105 cells/L to 94.92×105 cells/L in the experimental ponds. Among all experimental ponds, Chlorophyceae was found dominant (44.4×105 cells/L). Second dominant group was Euglenophyceae (39.6×105 cells/L) observed in pond-1 of Bismillah Agro Production (BAP). Total phytoplankton densities were recorded 47.82×105cells/L and 51×105 cells/L in pond-1 and pond-2 of Subarna Agro Based Initiative (SABI) respectively. In BAP, total phytoplankton densities were recorded 94.92×105cells/L and 36×105 cells/L in pond-1 and pond-2 respectively. Management technique and water quality parameters were also studied during study period namely water temperature, conductivity, salinity, transparency, dissolve oxygen and pH. The present study reveals that phytoplankton species are variable among the culture ponds and their density is also variable. The information provides for more research to compare water quality and pond phytoplankton characteristics in earthen aquaculture systems with and without fish stocking. Further studies on the seasonal changes of water quality parameters and its effects on phytoplankton production in the fish ponds and all year extended monitoring is recommended in future studies.
ADDITION OF ORGANIC WASTE ON NILE TILAPIA (OREOCHROMIS NILOTICUS) COMMERCIAL ...IAEME Publication
Nile tilapia (Oreochromis niloticus) is an important commodity in the aquaculture
of freshwater fish in Indonesia, apart from its distinctive taste but also a good protein
content for fulfilling community nutrition. However, the high demand for nile tilapia is
not enough just to rely on fishermen, so it takes effort to increase the growth rate of it.
This study aimed to determine the effect of shrimp waste and coconut pulp addition to
enhance protein retention, energy retention and growth rate of nile tilapia
(Oreochromis niloticus). This was an experimental study by means of completely
randomized design method. There were five treatments and four replications in each
treatment. The treatment used was the addition of shrimp waste and coconut pulp on
commercial feed. Two hundred nile tilapia were used in this study. The study was
conducted for 30 days by feeding three times a day. The research data were analyzed
using ANOVA and Duncan. Based on the results of the study, it can be seen that the
addition of 30% shrimp waste and coconut pulp flour to nile tilapia commercial feed
(Oreochromis niloticus) significantly affected the energy retention value of 12,050%,
protein retention of 21 245% and growth rate of 1.471%. Adding organic waste to
commercial fish feed can boost the growth rate of nile tilapia.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
Zooplankton species composition and diversity in the seagrass habitat of Lawa...AbdullaAlAsif1
Seagrass habitats are considered to be some of the most biodiverse ecosystems on the planet and safeguard some ecologically and economically important fauna, amongst which are some globally threatened species, including dugong. Malaysian seagrass ecosystems are not widespread, but their existence supports some significant marine fauna. A rigorous zooplankton study was conducted from May 2016 to February 2017, in the seagrass habitat of Lawas, Sarawak, Malaysia, to examine their temporal composition and diversity, together with their ecological influences. A total of 45 zooplankton species from 13 significant groups were recorded in the seagrass habitat. The population density of zooplankton ranged between 2,482 ind/m³ and 22,670 ind/m³ over three different seasons. A single zooplankton copepod was found to be dominant (47.40%), while bivalves were the second largest (31.8%) group in terms of total abundance. It was also noticed that the average relative abundance (0.62) and important species index (62.08) of copepods were higher than for other groups that exist in the seagrass meadow, whereas copepod Parvocalanus crassirostris showed both the highest average relative abundance (0.41) and the highest important species index (41.15). The diversity (H') and richness index of the intermediate season were found to be highest due to favourable physico-chemical conditions. Within the referred seasonal cluster, the wet and dry seasons were almost similar in terms of species abundance, while the intermediate season was distinct, with high species diversity backed by ANOSIM analysis results. Copepod and bivalves formed one group with a common similarity level of 0.80. The CCA (Canonical Correspondence Analysis) model established that abiotic factors, especially turbidity, NO , rainfall, dissolved oxygen and pH were significantly correlated with abundance of individual groups of zooplankton. Zooplankton assemblage and abundance in Lawas were found to be very rich in multiple seasons, indicating that the productivity of uninterrupted seagrass habitat might be high and the system rich in biodiversity.
DOI:10.21276/ijlssr.2016.2.4.19
ABSTRACT- Halophiles, the most predominant organisms found in the mangrove forest, include halophilic bacteria in different environment such as salt lakes, saline soils and salted food. The majo rviatyri eodf hgaelnoeprhai loicf pmriocproerotrigesa nwishmicsh s tfuadciielidta steo iftasr upsreo dwuicteh ccoommpmoeurncdias l waiitmh sg. rIena tt hpiost esntutidayl ionf inSduunsdterirabla pnr osoceils,s faoncdu st hheays hbaeveen pmhaydsieo loong itchael iasnodla wtieorne oufs ehda lfooprh pilhiyc soiorgcahnemismicsa la anndd t mheiicrr ochbaioralocgteicriasla atinoanl.y sSiosi. lQs uwaelritea tciovlel escctreede nfirnogms foofu trh ed iifsfoelraetnets pwlaecree sd oofn eS uanndd etrhbraene aCmhaornagc tethriesmat iowna so f stehlee citseodl ahteasv iwnegr em doodneer aatneldy bgaoseodd ognr o1w6Sth rRwNhiAch g ewnaes sefuqrutehnecr inogp tpimhyisloegde nine tidci ftfreeree nwt egrer ocwotnhs trmucetdeida.. aTsh we eisllo laast evsa rsihoouws eedx tmrauceltlilpullea rh eenazvyym mee ptarol dtoulcetriaonncse t haenrde bayn teixbpiolotirci nrge stihsetairn cues.a gTeh efo or rvgaarnioisums sb iwoeterceh fnuortlohgeric taels pteudr pfoosr eEs.P S Key-words- Sundarban, Halophiles, EPS, NaCl
Effect of water parameters on temporal distribution and abundance of zooplank...AbdullaAlAsif1
Seasonal abundance of zooplankton in relation to different physico-chemical conditions of Kaptai lake has been studied. A total of four major groups of zooplankton were identified from study area of Kaptai lake during three seasons observation at pre-monsoon, monsoon and Post-monsoon. These four groups were categorized into rotifers, copepods, cladocerans and others (fish larvae, shrimp larvae, insects, mosquito larvae etc.). The mean abundance of group rotifers, copepods, cladoceras and others were 103, 84.67, 38.33 and 41.83 ind. L-1 respectively. The highest group was rotifers in terms of abundance and composition where compositions were recorded 38 % at three season's observation. The lowest group was cladoceras in term of abundance and composition where compositions were recorded 14 %. The air temperature, water temperature, transparency, water pH, DO, BOD5, PO 4-P, NO 2-N, SiO 3-Si, TSS, TDS, Alkalinity ranged between 21 and 27.5C, 23.5 and 31.5C, 0.8 and 3 m, 7.1 and 7.5, 5.72 and 8.58 mg/l, 8.55 and 12.87 mg/l, 3.22 and 4.13 µg/l, 0.992 and 1.19 µg/l, 161.18 and 201.15 µg/l, 0.28 and 0.48 g/l, 0.18 and 0.68 g/l, 40 and 60 ppm, respectively. Margalef richness index (d), Pielou's evenness index (J'), Shannon-Wiener diversity index (H'), Simpson dominance index (λ) are analyzed by Primer V6 software to quantify the collected species. Through same software SIMPER, Cluster analysis also done to compare similarity between season.
Influence of Enclosure Conditions and Visitors on the Behavior of Captive Mal...KALAI ARASI
The population of Malayan tapirs (Tapirus indicus) in the wild is experiencing a radical decline mainly due to habitat destruction throughout their regions. Therefore, as an initiative measure to sustain the population, ex-situ conservation was established. However, the ability of captive management to maintain and breed endangered species has been proved challenging. In this study, we investigated how the behavior of Malayan tapirs in captivity is affected by enclosure conditions; type (semi-natural versus zoo enclosures/artificial) and weather (temperature and humidity), and visitors. Behaviors (categorized as resting, locomotion, ingestion, swimming, investigative) were observed using instantaneous sampling over 20 minute periods with intervals of 30 seconds and analyzed using generalized linear mixed-effects model, glmer. Enclosure type had a significant effect on feeding behavior where tapirs in semi-natural enclosures fed more frequently than tapirs in artificial environments, mirroring natural feeding activity. Significant adverse effects from background noises and visitors caused tapirs in artificial enclosure to be more alarmed and increased their investigative behaviors. High number of visitors overall lowered the activity of tapirs, while low humidity caused tapirs to suffer from dryness, and thus resulted in frequent ingestion (drinking) and locomotion behavior which indicative of thermal stress. Overall from this study, it is concluded that unsuitable enclosure conditions of extreme dryness and number of visitors are prone to be potential stressors that lower the activity pattern which possibly alter the natural behaviors of Malayan tapir in captivity. Therefore, further evaluation on exhibit design and management practices are encouraged to identify variables that could increase the well-being of captive Malayan tapirs.
Distribution of zooplankton community in Toli shad (Tenualosa toli) habitats,...AbdullaAlAsif1
Distribution of zooplankton community in Toli shad (Tenualosa toli) habitats, Sarawak, Malaysia. Biodiversitas 21: 4022-4033. The river ecosystems of Sarawak has been identified as important-spawning ground for economically important Toli shad (Tenualosa toli) fisheries. In these river ecosystems, zooplankton serves as a secondary producer to the different types of fish larvae including Toli shad. The productivity and biomass of zooplankton are important factors to control the production of higher trophic level organisms like fish larvae and fishes in different stages including T. toli larvae. In this viewpoint, the composition and abundance of zooplankton were investigated at 5 (five) sampling stations (considered to be spawning and nursing areas of Toli shad) at Batang Lupar and Batang Sadong estuaries from July 2016 to June 2017. A total of 29 zooplankton taxa were recorded that belong to 14 major groups comprised of Copepoda (Arthropoda), Crustacean nauplii (Arthropoda), Fish larvae (Chordata), Mollusca (Mollusca), Luciferidae (Arthropoda), Cnidaria (Cnidaria), Ostracoda (Arthropoda), Cirripedia (Arthropoda), Polychaete larvae (Annelida), Chaetognatha (Chaetognatha), Appendicularia (Chordata), Amphipoda (Arthropoda), Echinodermata larvae (Echinodermata) and Cladoceran (Arthropoda). Copepod was dominant group of zooplankton (82.40-94.41%) at all stations, contributing 477.37-25921.89 ind/m3 and 88.38% of total abundance. The second highest was crustacean larvae (2.45-9.38%) in all stations, contributing 0-2391.7 ind/m3 and 5.88% of total abundance. Zooplankton abundance was higher at downstream station 1 (8460.72 ind/m³) followed by station 2 (5479.80 indi/m³), station 3 (4571.95 ind/m³), station 4 (4394.20 ind/m³) and upstream station 5 (4156.73 ind/m³). Canonical Correspondence Analysis showed that the abundance of copepods was highly influenced by total turbidity and nitrate in the riverine and estuarine ecosystems. Abundance of zooplankton was relatively higher (9549.31 ind/m³) during the post-monsoon and lower (3534.56 ind/m³) during the southwest monsoon.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Fish to finance: unraveling the economic threads of Bangladesh’s Blue EconomyAbdullaAlAsif1
Bangladesh, with its extensive river network, vast coastlines, and rich maritime heritage, stands at the crossroads of a transformative Blue Economy. This comprehensive review explores the rich tapestry of Bangladesh's maritime journey. Commencing with a historical lens, it traces the nation's roots in fisheries, showcasing its transformation into a diversified and dynamic Blue Economy. The significance of this transition unfolds across the review, underscoring the sector's contributions to GDP, employment, and food security, while candidly addressing challenges such as overfishing and environmental impact. This work not only chronicles historical milestones but distills valuable lessons from both successes and setbacks, offering a nuanced understanding of the economic resilience embedded in Bangladesh's fisheries sector. Beyond a historical retrospective, the review navigates through the contemporary landscape, emphasizing diversification beyond fisheries. It illuminates economic opportunities in maritime trade, explores non-fish marine resources, and delves into the transformative role of aquaculture. The policy framework governing Bangladesh's Blue Economy comes into focus, dissecting government strategies, legal landscapes, and international collaborations. The review also champions environmental sustainability, highlighting the impact of Blue Economy activities on marine ecosystems and advocating for conservation measures, thus positioning Bangladesh as a steward of its rich maritime heritage. Financial mechanisms and investments emerge as pivotal elements, shaping the trajectory of Blue Economy projects, as evidenced by case studies that weave success stories and lessons learned into the narrative. Concluding with a forward-looking perspective, the study identifies emerging trends, growth opportunities, and strategies to tackle challenges, offering a comprehensive roadmap for sustainable development. In weaving together economic vibrancy, environmental stewardship, and future aspirations, this review illuminates Bangladesh's maritime odyssey as a beacon for the global Blue Economy community.
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Productivity of phytoplankton using different organic fertilizers in the glas...AbdullaAlAsif1
The experiment intended to estimate the productivity using different organic fertilizer and to identify the species of phytoplankton in the aquarium tanks. The study was conducted in the laboratory Phytoplankton samples for culture were collected from fisheries experimental pond by using conical-shaped monofilament nylon net (phytoplankton net). Three treatment such as cow dung (T₁), chicken manure (T₂) and control (T₃) were designed. Each treatment had two replications. In case of T₁ and T₂, 10 g of fertilizer was used in each aquarium tank. Sampling of phytoplankton for counting and identification was done every week and 1 litre of water sample was taken from each tank. Phytoplankton sample for identification were preserved by Lugol's solution. Three physicochemical parameters namely water temperature, dissolved oxygen (DO) and pH were measured in every sampling week during the study period. Water quality parameters of the aquarium tank varied with the variation of sampling week. There was no significant difference between water temperature, pH and DO in case of three treatment. The mean water temperature, pH and DO were 21.85±1.95 °C, 8.38±0.23 and 5.55±0.58 mg/l, respectively. The abundance of phytoplankton in T₁, T₂ and T₃ were 25-65, 25-105 and 6-20 individual/l, respectively. The phytoplankton abundance were influenced by different organic fertilizer and noticed higher in number by using chicken manure. Irregular relationship between phytoplankton abundance and water temperature was found. Phytoplankton showed positive relationship with DO. In this study 15 species of phytoplankton were identified under four groups namely Chlorophyta, Cyanophyta, Bacillariophyta and Euglenophyta. Chlorophyta (52%) was the dominant group in the aquarium. The findings of the present study will help to improve the management strategies of water quality, for estimating the productivity of phytoplankton and for the best use of organic fertilizer especially chicken manure.
The effects of different water quality parameters on zooplankton distribution...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online
The Growth of Oreochromis niloticus (2.6g initial average size) was studied in 100m2 earthen ponds for 180 days under tropical field environment. Varying fertilizing regimes consisting of Pennisetum purpureum (PP) and Musa sapientum (MS), two locally available weedy grasses were applied in duplicates in the experimental ponds, at the rate of 0.1-0.2 kg dry matter per m2 per day. Water quality parameters were monitored bimonthly between 8:00am-9:00am . Final average fish weight varied significantly (p<0.05) as follows: 43.85g, 35.5g, 59.5g, 24.9g and 50g, 2 respectively for PP, MS, MS+PP, T0 and T1. Corresponding daily growth were 2.2g/d, 0.1g/d, 0.3g/d, 0.1g/d and 0.2g/d. Survival rate equally varied significantly as follow: 60 %, 67 %, and 98 %, 70 % and 97 % respectively. During the study period, the results indicated the water quality were permissibole limits and can be used for livestock of Oreochromis niloticus .
A potential endemic cyprinid for aquaculture and food security: The embryonic...AbdullaAlAsif1
The Bornean Spotted Barb is endemic fish species and widely distributed throughout Borneo in various freshwater bodies and can be considered as a potential aquaculture candidate for food security and the ornamental trade. However there has yet to be scientific studies on the early life history and developmental stages of this species. Thus the present study reveals the embryonic development of Puntius sealei embryo, from fertilization to post-hatching using the induced breeding method. The induced spawning on P. sealei using Ovaprim hormone at a dose of 0.5 ml/kg for females and 0.25ml/kg for males respectively was successfully conducted and the embryonic development of the fertilized eggs were observed. The zygote, cleavage, blastula, gastrula, segmentation, and larval phases were the six major stages of embryogenesis that investigated in this experiment. This study contributed major knowledge to the early developmental biology of this particular species. The information will be useful for accessing the potentiality of this species for aquaculture as well as the management, and production of the species.
Growth of freshwater mud eel (Monopterus cuchia) in different water condition...AbdullaAlAsif1
An experiment was conducted to study the effect of different feeds on growth and production of freshwater Mud Eel (Monopterus cuchia) in nine aquariums over a period of 90 days during June to August 2016. Three different feeds namely Feed A (live feed), Feed B (supplementary feed) and Feed C (probiotics) were applied to treatments I, II and III respectively. Three types of water used in aquarium. These are pond water, ground water and turbid water. Each of the tanks was stocked with nine fish that's total fish amount of 27. Significantly highest weight gain was obtained in pond water, treatment III, (16.39a ± 2.35). Treatment III yielded the highest (65.56a ±9.4 g/m 2 /year) production with the highest survival rate of fish. Water quality parameters like temperature, dissolved oxygen, P H , and alkalinity were optimum. On the basis of better growth and production, it is suggested that pond water condition with probiotics are suitable for the culture of M. cuchia in aquarium.
Information about composition of deep-sea edible part might raise their value as table food. Proximate composition of few fin fish species from North Atlantic, eastern North Pacific has been reported. No literature is available for either fin fish or shell fish from deep waters off the Indian EEZ.
Evaluation of the oyster farming potential of the Cintra bay (southern Morocco)Origins publication
The bay of Cintra is a marine ecosystem located in the southern Moroccan Atlantic known for its
biological richness and therefore for its high fishery productivity. This bay was chosen as a new destination for the
development of aquaculture in the southern regions of Morocco. To highlight its potential in terms of oyster
farming, a first rearing trial of triploid cupped oysters (Crassostrea gigas) as well as parallel monitoring of the
phytoplankton population and ecological parameters were undertaken. The results obtained showed that the oysters
adapt well to the conditions of the new environment where mortality was negligible, or even absent after a month
of launching. In terms of biological performance, the growth of individuals is continuous during the annual cycle
and after eight months the weight could reach 30.5 g which is a minimum weight for marketing. The AFNOR and
Lawrence and Scott indices showed a good physiological state and a better commercial quality of the oysters. The
filling rate of these oysters, with an average weight of 39.7g when lifting, is very high according to the Lawrence and
Scott index (126.3) and the average AFNOR index (around 21.8) rank them in the “Special” category.
Use of liquid rice starch as a source of carbon for growth of Spirulina plate...AbdullaAlAsif1
A study was conducted to examine the culture and growth performance of S. platensis in three different concentrations (25%, 50% and 100%) of digested liquid rice starch media (DLRSM) with 0.2 g/L urea and Kosaric medium (KM) as control for a period of three months from March to May 2018. Each experiment was done in triplicates for a period of 14 days. The growth rate of S. platensis was found to vary with different concentrations of the medium. The cell weight of S. platensis was attained to highest 12.42 mg/L (dry weight) in KM followed by 11.26 mg/L, 8.35 mg/L and 9.11 mg/L in 25%, 50% and 100% of DLRSM, respectively on the 10 th day of culture period. Similar trend also observed in case of chlorophyll a content of S. platensis. The proximate composition of rice starch was analyzed. The percentage of moisture, protein, lipid, ash, crude fiber and nitrogen free extract (NFE) were95.1, 0.2616, 0.1055, 0.1097, 0.1468 and 3.9669, respectively on the basis of moisture (%) and 4.59, 5.70, 2.30, 2.39, 3.20 and 86.41, respectively on the basis of dry matter (%). The results on the growth performance showed that the growth of S. platensis was significantly (P<0.01) higher when grown in 25% concentration of DLRSM than other concentrations in 50% and 100% of DLRSM. The physico-chemical parameters viz. light intensity (2685 to 2773 lux/m 2 /s), temperature (29.6 to 31.5°C), pH (8.3 to 9.9), alkalinity (1150 to 3032 mg/L), Nitrate-N (1.32 to 6.30 mg/L) and phosphate-P (9.75 to 61.50 mg/L) were within optimum level during the culture period.
ABSTRACT- We conducted a first order analysis on the proximate composition (protein, carbohydrate, fat and astaxanthin) of three dominant seaweed species viz. Enteromorpha intestinalis, Ulva lactuca and Catenella repens inhabiting Indian Sundarbans. The study was conducted at three stations (Gosaba, Bali Island and Jharkhali) during premonsoon, monsoon and postmonsoon of 2014-15. The relevant hydrological parameters (surface water temperature, salinity, pH, dissolved oxygen and dissolved nutrients) were monitored simultaneously during the tenure of the work. ANOVA carried out on the observed data reflects pronounced variations of all hydrological parameters except surface water temperature and salinity between stations. Pronounced seasonal variations were observed for all the selected hydrological parameters. In the domain of proximate composition, ANOVA results exhibit pronounced variations between stations and seasons (except carbohydrate in U. lactuca and C. repens between stations and astaxanthin in U. lactuca between seasons).
Keywords - Seaweed, Indian Sundarbans, Proximate composition, ANOVA, Seasonal variation
Qualitative and quantitative analysis of phytoplankton in culture pond of Noa...AbdullaAlAsif1
A study was conducted on qualitative and quantitative analysis of phytoplankton in cultured ponds of two fish farms of Noakhali district, Bangladesh from 15 September to 15 November 2012 to identify and estimate the abundance of phytoplankton in various culture ponds of two fish farms. Analyses of phytoplankton samples recorded a total of 4 classes phytoplankton viz.; Bacillariophyceae, Chlorophyceae, Cyanophyceae, Euglenophyceae. Out of 21 phytoplankton genera identified, 5 belong to Cyanophyceae, 7 to Chlorophyceae, 5 to Bacillariophyceae and 4 to Euglenophyceae. Among the identified genera, Euglena, Microcystis, Eurolena were found to be dominant genera. Total phytoplankton abundance was varied from 36×105 cells/L to 94.92×105 cells/L in the experimental ponds. Among all experimental ponds, Chlorophyceae was found dominant (44.4×105 cells/L). Second dominant group was Euglenophyceae (39.6×105 cells/L) observed in pond-1 of Bismillah Agro Production (BAP). Total phytoplankton densities were recorded 47.82×105cells/L and 51×105 cells/L in pond-1 and pond-2 of Subarna Agro Based Initiative (SABI) respectively. In BAP, total phytoplankton densities were recorded 94.92×105cells/L and 36×105 cells/L in pond-1 and pond-2 respectively. Management technique and water quality parameters were also studied during study period namely water temperature, conductivity, salinity, transparency, dissolve oxygen and pH. The present study reveals that phytoplankton species are variable among the culture ponds and their density is also variable. The information provides for more research to compare water quality and pond phytoplankton characteristics in earthen aquaculture systems with and without fish stocking. Further studies on the seasonal changes of water quality parameters and its effects on phytoplankton production in the fish ponds and all year extended monitoring is recommended in future studies.
ADDITION OF ORGANIC WASTE ON NILE TILAPIA (OREOCHROMIS NILOTICUS) COMMERCIAL ...IAEME Publication
Nile tilapia (Oreochromis niloticus) is an important commodity in the aquaculture
of freshwater fish in Indonesia, apart from its distinctive taste but also a good protein
content for fulfilling community nutrition. However, the high demand for nile tilapia is
not enough just to rely on fishermen, so it takes effort to increase the growth rate of it.
This study aimed to determine the effect of shrimp waste and coconut pulp addition to
enhance protein retention, energy retention and growth rate of nile tilapia
(Oreochromis niloticus). This was an experimental study by means of completely
randomized design method. There were five treatments and four replications in each
treatment. The treatment used was the addition of shrimp waste and coconut pulp on
commercial feed. Two hundred nile tilapia were used in this study. The study was
conducted for 30 days by feeding three times a day. The research data were analyzed
using ANOVA and Duncan. Based on the results of the study, it can be seen that the
addition of 30% shrimp waste and coconut pulp flour to nile tilapia commercial feed
(Oreochromis niloticus) significantly affected the energy retention value of 12,050%,
protein retention of 21 245% and growth rate of 1.471%. Adding organic waste to
commercial fish feed can boost the growth rate of nile tilapia.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
Zooplankton species composition and diversity in the seagrass habitat of Lawa...AbdullaAlAsif1
Seagrass habitats are considered to be some of the most biodiverse ecosystems on the planet and safeguard some ecologically and economically important fauna, amongst which are some globally threatened species, including dugong. Malaysian seagrass ecosystems are not widespread, but their existence supports some significant marine fauna. A rigorous zooplankton study was conducted from May 2016 to February 2017, in the seagrass habitat of Lawas, Sarawak, Malaysia, to examine their temporal composition and diversity, together with their ecological influences. A total of 45 zooplankton species from 13 significant groups were recorded in the seagrass habitat. The population density of zooplankton ranged between 2,482 ind/m³ and 22,670 ind/m³ over three different seasons. A single zooplankton copepod was found to be dominant (47.40%), while bivalves were the second largest (31.8%) group in terms of total abundance. It was also noticed that the average relative abundance (0.62) and important species index (62.08) of copepods were higher than for other groups that exist in the seagrass meadow, whereas copepod Parvocalanus crassirostris showed both the highest average relative abundance (0.41) and the highest important species index (41.15). The diversity (H') and richness index of the intermediate season were found to be highest due to favourable physico-chemical conditions. Within the referred seasonal cluster, the wet and dry seasons were almost similar in terms of species abundance, while the intermediate season was distinct, with high species diversity backed by ANOSIM analysis results. Copepod and bivalves formed one group with a common similarity level of 0.80. The CCA (Canonical Correspondence Analysis) model established that abiotic factors, especially turbidity, NO , rainfall, dissolved oxygen and pH were significantly correlated with abundance of individual groups of zooplankton. Zooplankton assemblage and abundance in Lawas were found to be very rich in multiple seasons, indicating that the productivity of uninterrupted seagrass habitat might be high and the system rich in biodiversity.
DOI:10.21276/ijlssr.2016.2.4.19
ABSTRACT- Halophiles, the most predominant organisms found in the mangrove forest, include halophilic bacteria in different environment such as salt lakes, saline soils and salted food. The majo rviatyri eodf hgaelnoeprhai loicf pmriocproerotrigesa nwishmicsh s tfuadciielidta steo iftasr upsreo dwuicteh ccoommpmoeurncdias l waiitmh sg. rIena tt hpiost esntutidayl ionf inSduunsdterirabla pnr osoceils,s faoncdu st hheays hbaeveen pmhaydsieo loong itchael iasnodla wtieorne oufs ehda lfooprh pilhiyc soiorgcahnemismicsa la anndd t mheiicrr ochbaioralocgteicriasla atinoanl.y sSiosi. lQs uwaelritea tciovlel escctreede nfirnogms foofu trh ed iifsfoelraetnets pwlaecree sd oofn eS uanndd etrhbraene aCmhaornagc tethriesmat iowna so f stehlee citseodl ahteasv iwnegr em doodneer aatneldy bgaoseodd ognr o1w6Sth rRwNhiAch g ewnaes sefuqrutehnecr inogp tpimhyisloegde nine tidci ftfreeree nwt egrer ocwotnhs trmucetdeida.. aTsh we eisllo laast evsa rsihoouws eedx tmrauceltlilpullea rh eenazvyym mee ptarol dtoulcetriaonncse t haenrde bayn teixbpiolotirci nrge stihsetairn cues.a gTeh efo or rvgaarnioisums sb iwoeterceh fnuortlohgeric taels pteudr pfoosr eEs.P S Key-words- Sundarban, Halophiles, EPS, NaCl
Effect of water parameters on temporal distribution and abundance of zooplank...AbdullaAlAsif1
Seasonal abundance of zooplankton in relation to different physico-chemical conditions of Kaptai lake has been studied. A total of four major groups of zooplankton were identified from study area of Kaptai lake during three seasons observation at pre-monsoon, monsoon and Post-monsoon. These four groups were categorized into rotifers, copepods, cladocerans and others (fish larvae, shrimp larvae, insects, mosquito larvae etc.). The mean abundance of group rotifers, copepods, cladoceras and others were 103, 84.67, 38.33 and 41.83 ind. L-1 respectively. The highest group was rotifers in terms of abundance and composition where compositions were recorded 38 % at three season's observation. The lowest group was cladoceras in term of abundance and composition where compositions were recorded 14 %. The air temperature, water temperature, transparency, water pH, DO, BOD5, PO 4-P, NO 2-N, SiO 3-Si, TSS, TDS, Alkalinity ranged between 21 and 27.5C, 23.5 and 31.5C, 0.8 and 3 m, 7.1 and 7.5, 5.72 and 8.58 mg/l, 8.55 and 12.87 mg/l, 3.22 and 4.13 µg/l, 0.992 and 1.19 µg/l, 161.18 and 201.15 µg/l, 0.28 and 0.48 g/l, 0.18 and 0.68 g/l, 40 and 60 ppm, respectively. Margalef richness index (d), Pielou's evenness index (J'), Shannon-Wiener diversity index (H'), Simpson dominance index (λ) are analyzed by Primer V6 software to quantify the collected species. Through same software SIMPER, Cluster analysis also done to compare similarity between season.
Influence of Enclosure Conditions and Visitors on the Behavior of Captive Mal...KALAI ARASI
The population of Malayan tapirs (Tapirus indicus) in the wild is experiencing a radical decline mainly due to habitat destruction throughout their regions. Therefore, as an initiative measure to sustain the population, ex-situ conservation was established. However, the ability of captive management to maintain and breed endangered species has been proved challenging. In this study, we investigated how the behavior of Malayan tapirs in captivity is affected by enclosure conditions; type (semi-natural versus zoo enclosures/artificial) and weather (temperature and humidity), and visitors. Behaviors (categorized as resting, locomotion, ingestion, swimming, investigative) were observed using instantaneous sampling over 20 minute periods with intervals of 30 seconds and analyzed using generalized linear mixed-effects model, glmer. Enclosure type had a significant effect on feeding behavior where tapirs in semi-natural enclosures fed more frequently than tapirs in artificial environments, mirroring natural feeding activity. Significant adverse effects from background noises and visitors caused tapirs in artificial enclosure to be more alarmed and increased their investigative behaviors. High number of visitors overall lowered the activity of tapirs, while low humidity caused tapirs to suffer from dryness, and thus resulted in frequent ingestion (drinking) and locomotion behavior which indicative of thermal stress. Overall from this study, it is concluded that unsuitable enclosure conditions of extreme dryness and number of visitors are prone to be potential stressors that lower the activity pattern which possibly alter the natural behaviors of Malayan tapir in captivity. Therefore, further evaluation on exhibit design and management practices are encouraged to identify variables that could increase the well-being of captive Malayan tapirs.
Distribution of zooplankton community in Toli shad (Tenualosa toli) habitats,...AbdullaAlAsif1
Distribution of zooplankton community in Toli shad (Tenualosa toli) habitats, Sarawak, Malaysia. Biodiversitas 21: 4022-4033. The river ecosystems of Sarawak has been identified as important-spawning ground for economically important Toli shad (Tenualosa toli) fisheries. In these river ecosystems, zooplankton serves as a secondary producer to the different types of fish larvae including Toli shad. The productivity and biomass of zooplankton are important factors to control the production of higher trophic level organisms like fish larvae and fishes in different stages including T. toli larvae. In this viewpoint, the composition and abundance of zooplankton were investigated at 5 (five) sampling stations (considered to be spawning and nursing areas of Toli shad) at Batang Lupar and Batang Sadong estuaries from July 2016 to June 2017. A total of 29 zooplankton taxa were recorded that belong to 14 major groups comprised of Copepoda (Arthropoda), Crustacean nauplii (Arthropoda), Fish larvae (Chordata), Mollusca (Mollusca), Luciferidae (Arthropoda), Cnidaria (Cnidaria), Ostracoda (Arthropoda), Cirripedia (Arthropoda), Polychaete larvae (Annelida), Chaetognatha (Chaetognatha), Appendicularia (Chordata), Amphipoda (Arthropoda), Echinodermata larvae (Echinodermata) and Cladoceran (Arthropoda). Copepod was dominant group of zooplankton (82.40-94.41%) at all stations, contributing 477.37-25921.89 ind/m3 and 88.38% of total abundance. The second highest was crustacean larvae (2.45-9.38%) in all stations, contributing 0-2391.7 ind/m3 and 5.88% of total abundance. Zooplankton abundance was higher at downstream station 1 (8460.72 ind/m³) followed by station 2 (5479.80 indi/m³), station 3 (4571.95 ind/m³), station 4 (4394.20 ind/m³) and upstream station 5 (4156.73 ind/m³). Canonical Correspondence Analysis showed that the abundance of copepods was highly influenced by total turbidity and nitrate in the riverine and estuarine ecosystems. Abundance of zooplankton was relatively higher (9549.31 ind/m³) during the post-monsoon and lower (3534.56 ind/m³) during the southwest monsoon.
Similar to Proximate and mineral composition of the long-spined Sea Urchin (Diadema setosum) roe (20)
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Fish to finance: unraveling the economic threads of Bangladesh’s Blue EconomyAbdullaAlAsif1
Bangladesh, with its extensive river network, vast coastlines, and rich maritime heritage, stands at the crossroads of a transformative Blue Economy. This comprehensive review explores the rich tapestry of Bangladesh's maritime journey. Commencing with a historical lens, it traces the nation's roots in fisheries, showcasing its transformation into a diversified and dynamic Blue Economy. The significance of this transition unfolds across the review, underscoring the sector's contributions to GDP, employment, and food security, while candidly addressing challenges such as overfishing and environmental impact. This work not only chronicles historical milestones but distills valuable lessons from both successes and setbacks, offering a nuanced understanding of the economic resilience embedded in Bangladesh's fisheries sector. Beyond a historical retrospective, the review navigates through the contemporary landscape, emphasizing diversification beyond fisheries. It illuminates economic opportunities in maritime trade, explores non-fish marine resources, and delves into the transformative role of aquaculture. The policy framework governing Bangladesh's Blue Economy comes into focus, dissecting government strategies, legal landscapes, and international collaborations. The review also champions environmental sustainability, highlighting the impact of Blue Economy activities on marine ecosystems and advocating for conservation measures, thus positioning Bangladesh as a steward of its rich maritime heritage. Financial mechanisms and investments emerge as pivotal elements, shaping the trajectory of Blue Economy projects, as evidenced by case studies that weave success stories and lessons learned into the narrative. Concluding with a forward-looking perspective, the study identifies emerging trends, growth opportunities, and strategies to tackle challenges, offering a comprehensive roadmap for sustainable development. In weaving together economic vibrancy, environmental stewardship, and future aspirations, this review illuminates Bangladesh's maritime odyssey as a beacon for the global Blue Economy community.
Successful hybridization between Clarias microstomus♂ and Clarias gariepinus♀AbdullaAlAsif1
Hybridization is a significant approach to breeding that enables the creation of new varieties that enhance genetic diversity. Through fish inter-species hybridization, it is possible to produce hybrids that can be utilized in aquaculture and stocking programs to enhance growth rate, transfer desirable traits between species, and combine the favourable attributes of two parents into a single progeny. The present study revealed the successful hybridization between Clarias microstomus (Ng, 2001) ♂ and Clarias gariepinus (Burchell, 1822) ♀ for the first time in the aquaculture industry. C. microstomus is a species of Clariid catfish endemic to the island of Borneo and C. gariepinus is an introduced species widely aquacultured throughout the southeast Asian region. The embryonic development of the hybrid offspring is described with the hatching percentage of 58.63% and the early survival rate for the first 72 hours for the larvae of 85.76%. The hybrids showed no signs of deformities and developed normally. The findings of the study provide a new high yield variety of catfish for farmers and aquaculture industry for increasing production and profit margin.
Freshwater gastropod diversity in the selected lotic environment, Betong, Sar...AbdullaAlAsif1
Freshwater Gastropoda can be considered as the biological indicator in ecosystems such as rivers, streams, lakes, and ponds. The objectives of this study are to identify freshwater gastropod species, diversity, and distribution along Sungai Penebak, Sungai Nanga Tiga, and Sungai Kabo in the Betong division. The study was conducted on 10 November 2020. A 50 m transect was laid on the riverbank of all three stations. The existing specimens were counted and collected from each sampling point and stored were taken to the laboratory for species identification. The diversity index and morphological study of freshwater gastropods were performed. Five species of freshwater gastropods belonging to four families were discovered. The five species of freshwater gastropods consisted of Sulcospira pageli, which shows great abundance, followed by Clea nigricans, Brotia costula, Pila ampullacea, and Vittina pennata. The diversity indices of collected Gastropoda species from the different stations, for instance, Shannon Weiner diversity index (H’), Pielou’s evenness index (J’), and Margalef’s richness index were assessed; where station 3 showed higher diversity of Gastropoda compared to other two stations. The information presented in this paper might be helpful for ecological wealth studies and considered as the baseline data for the stream ecosystem in Sarawak, Malaysia.
Macrofaunal diversity on the surface sediment of mangrove habitat adjacent to...AbdullaAlAsif1
Macrofaunal diversity and composition are essential to understanding the ecosystem structures and functions of mangroves in any dynamic estuarine coast. To understand the temporal distribution of macrofaunal community structures and diversity on the surface sediment in the intertidal mangrove forest next to the seagrass beds, this study was conducted at Punang-Sari-River estuary, Lawas, Sarawak (Malaysian Borneo), South China Sea. Sampling was done during post-monsoon, intermediate-September, premonsoon, and monsoon from July 2019 to February 2020. The mean macrofauna abundance was higher pre-monsoon, while Gastropoda Optediceros breviculum (L. Pfeiffer, 1855) was the most abundant (RA = 0.704). Important species index (ISI = 70.36) and percentage of contribution (64.97%) were also higher of this species than any other records from the site. The ANOSIM (Analysis of Similarity), Jaccard index and SIMPER (Similarity Percentage Analysis) analysis suggested that the highest species abundance similarity was observed between pre-monsoon and monsoon, while the highest dissimilarity was observed between intermediate-September and pre-monsoon (74.67%). Seasonal PCA, nMDS, and cluster analysis revealed that pre-monsoon and monsoon were comparable in species and individual abundance. The dominance (0.85) was found significantly higher (P < 0.0001) in intermediate-September, while Simpson (0.734), Shannon (1.56) and Evenness indices (0.528) were found higher in monsoon, followed by the higher Margalef richness indices (3.21) in post-monsoon. It was observed that the seasonal ecological parameters were found to be significantly (P<0.05) different, including the temperature, salinity, rainfall, pore water nitrogenous compounds (NO2, NO3 and NH3-N), phosphorus compound (PO4) and micro minerals. The Canonical Correspondence Analysis (CCA) results suggested that ecological factors influenced several species. Considering the few studies on mangrove macrofauna in Malaysian Borneo, further research on their trophic structures and food web interactions is very important to disclose their overall characteristics and ecosystem functions.
Population characteristics of the Japanese threadfin bream Nemipterus japonic...AbdullaAlAsif1
Nemipterus japonicus (Japanese threadfin bream) is one of the most significant fish species throughout the Indo-Pacific regions. No previous studies on the population dynamics of N. japonicus have been carried out in the Eastern Malaysia (Malaysian Borneo). The present study focused on the population dynamics of N. japonicus from April 2013 to March 2014 in the coastal area of Bintulu, South China Sea. Local fishermen provided samples, which were then analysed. The FiSAT II software was used to evaluate the collected length frequency data. The asymptotic length (L ∞) and growth coefficient (K) were 26.78 cm and 0.85 yr-1, respectively. The growth performance index (ϕ'), total mortality coefficient (Z), natural mortality (M) and fishing mortality (F) were 2.785, 2.97, 1.63 and 1.34 per year, respectively. According to the estimates, the exploitation rate (E) for N. japonicus was 0.45, while the E 0.1 , E 0.5 and E max were determined as 0.72, 0.4 and 0.806, respectively. The obtained value of exploitation was less than E max, although slightly higher than E 0.5. The recruitment characteristics of N. japonicus have been stable throughout the year, with one peak pulse. The results contribute to the knowledge of the dynamics of N. japonicus populations and are important for the sustainable management of the Sarawak fishing resources.
Trends in seagrass research and conservation in Malaysian watersAbdullaAlAsif1
The seagrass ecosystems found in the marine and coastal areas, with substantial economic and ecological services and span all over the globe excluding the Antarctic region. The Coral Triangle and Southeast Asia are recognized as a worldwide hotspot of seagrass species and habitats, encompassing 10-21 species of seagrass in every nation , although the study, understanding, and quantity of publications on seagrass ecosystems are rather limited in the region, including Malaysia. Malaysia contains 18 seagrass species from three families, which occupy 16.8 km 2 of coastal area, where the study and discovery of seagrass species and meadows began in 1904 with the report of Beccari. All of the published papers reviewed reported on Malaysian seagrass-related research, which was divided into nine topic groups: biology and distribution, carbon sequestration, fauna, remote sensing, impact and pollution genetic study, restoration , microbiological investigation, and others. The extensive study of the seagrass ecosystem began in 1993, and we have identified 183 published papers from Scopus, 141 publications from Web of Science, and 42 from Google Scholar. However, the average trend of the number of publications from 1993 to 1999 was 0.71 ± 0.36, while from 2000 to 2022 was 7.70 ± 1.16 followed by the average trend of the yearly number of publications was 6.78 ± 1.08. The highest number of publications was found on faunal categories (43.17%), followed by biology and distribution (21.85%). The number of articles that were published on Malaysian seagrass meadows each year has been discovered to be rising, which indicates that the trends in seagrass study and publishing were progressively garnering the attention of researchers, academics, and the government. However, to better understand the sustainable ecology and ecosystem services provided by seagrass habitats, an emphasis on certain research niches, such as the genetic study of flora and fauna in seagrass meadows, microbial ecology, and restoration as well as conservation of seagrass species might be helpful.
First record of saucer scallop Ylistrum balloti (Bernardi, 1861) from equator...AbdullaAlAsif1
Ylistrum balloti is one of the Pectinidae species distributed within the Indo-Pacific region. Recently, 15 live specimens of Y. balloti were recorded from the continental shelf of Sarawak, Malaysia. The main morphological characteristics were displayed on the outer valve, these being a brown-red colour and a clear concentric pattern of thin brown lines. The prominent internal ribbing numbers on both valves also helped species identification. The preliminary report of Y. balloti revealed that the species is present in Sarawak waters and can be further explored in the future.
Culture and production of Lucilia sericata Meigen (1826) larvae for rearing s...AbdullaAlAsif1
The fly larvae (Lucilia sericata) are being used as an alternative protein source in any kind of animal feed as it reduces the cost of preparation of feed. This current study focused on the physico-chemical and proximate properties of raw poultry waste, while this study also revealed the production process of fly larvae and the proximate composition of it. The poultry waste was collected from Suvro poultry farm, Sutiakhali, Mymensingh, then the physic-chemical properties of the raw poultry waste were determined using different procedures. Three treatments were considered for production of fly larvae, T 1 (3 kg), T 2 (6 kg) and T 3 (9 kg) in a 15 kg capacity tray with three replications. After production, the proximate composition were also measured of fly larvae. Physico-chemical properties such as, color, odor, texture, temperature, pH, total solids (TSS+TDS), chemical oxygen demand, dissolved oxygen, alkalinity, available N, available P, and fiber in raw poultry waste were determined and presented. Proximate composition such as, moisture, total N, total P, available N, total Ca, ash and crude fiber were found in significant amount in the poultry waste. It was found that T 3 (1350±68g) produced highest volume of live maggot compared to the other treatments, but T 2 (17.50±1.10%) produced highest percentage volume. The proximate compositions of fly larvae were assessed and found 56.60±0.25% protein value in it, suggesting that fly larvae could be the protein replacer in fish feed. The result of this study revealed cheap protein source in aquaculture production, such as production and rearing of stinging catfish Heteropneustes fossilis, and the findings might be helpful for cost reduction in aquaculture operation.
Replacement of fish meal by Lucilia sericata (Meigen, 1826) live larvae and p...AbdullaAlAsif1
Reduction of aquaculture operations and production cost is key to the success and profitability of this enterprise, while protein needs, especially the cost of fish meal, are cery expensive. The current study was conducted to investigate the post-larval production performance of Heteropneustes fossilis using Lucilia sericata maggot as a fish meal replacer in two ways, namely, live larvae and powder form. A 28 days growth trial was performed where five isonitrogenous diets for Heteropneustes fossilis post-larvae were experimented using live maggots and maggot meal, respectively. The proximate composition of each formulated diet, growth parameters of fish post-larvae, such as, weight gain, specific growth rate, protein efficiency ratio, apparent protein utilization, survival rate and the food conversion ratio were examined. After the experiment, the carcass composition of the experimental fishes were evaluated. The best final weight (1.61 g), weight gain (1.418 g), percentage of weight gain (739±1.18%), specific growth rate (2.63), protein efficiency ratio (2.29), apparent protein utilization (85%), survival rate (90%) and lower food conversion ratio (2.06) were observed in fish fed with 75% maggot meal as a substitute of fish meal. This study will help the aquaculture industry, especially the catfish culture in identifying an alternate source of protein and lowering the cost of aquaculture operation.
The molecular approach reveals the relationship among Venus clams (Meretrix s...AbdullaAlAsif1
Molecular study is important to detect variations and similarities among species from the same genus, in case if they do not encompass any morphological or physiological differences. The study was conducted to differentiate among species of Meretrix spp. (Meretrix lyrata, M. meretrix, and M. lusoria) obtained from two locations in Malaysia through the phylogenetic tree. The adductor muscle tissues were used to extract DNA and to perform other procedures; the samples were subjected to analyses using PCR and gel electrophoresis. The multiple sequence comparison was conducted by MUSCLE and the phylogenetic relationships were established using Maximum Likelihood (ML) statistical methods with MEGA 6.0 statistical software. M. lyrata samples showed 99% similarity to the three accessions sequence, where M. lyrata indicated 87% similarities, and M. meretrix showed not more than 89% similarities from the deposited sequence. The nucleotide base composition sequences consisted of the mean of Thiamine (T) 37.9%, Cytosine (C) 15.4%, Adenine (A) 27.4%, and Guanine (G) 19.4%. Maximum Likelihood (ML) analysis was conducted using the Tamura 3-parameter model to establish five major clades on Meretrix spp. and two out-groups clades significantly different from the Meretrix spp. These major clades were closely related to each other at the 50% evidence of bootstrap, which grouped as genus Meretrix. The present study on Meretrix spp. from the Sarawak locality was able to differentiate COI sequences between M. lyrata, M. meretrix, and M. lusoria. M. lusoria was close related to M. meretrix with strong bootstrap supporting evidence at 96% scoring. Moreover, M. lyrata was inferred as the ancestor to M. meretrix, and M. lusoria from Sarawak, Malaysia.
Disappearance or overlooked or untouched? - A brief history of aquatic gastro...AbdullaAlAsif1
The aquatic gastropods (marine and freshwater) in Malaysian Borneo (MYB) have an ancient history of 255 years, while the first record was observed in 1767 by the pioneer Carl Linnaeus. Over time, many European, native taxonomists and conservationists have worked on the diverse group of gastropods in the Malaysian part of Borneo (states of Sabah and Sarawak). No previous work has been conducted to assess the historical status of this particular class of Mollusca. The study objective was to reveal the types of aquatic gastropods and the number of published research and expeditions conducted in MYB. The bibliometric analysis found 145 research publications mentioning the names of the region and reporting at least one gastropod species from this geographic region. Over the history of 255 years, a total of 559 gastropod species were reported by different research groups. Between 1767 and 1900, a total of 150 gastropod species were reported, followed by 79 species from 1900 to 2000 and 330 species from 2001 to 2022. The Grubbs test (p<0.05) identified outlier years for the reporting of gastropod species from MYB with 54 new species each in 2011 and 2020 and 39 species in 2001. Over the history of aquatic gastropod research in MYB, the taxonomists making the greatest contribution were Han Raven and his colleagues from Naturalis who reported 111 new species from the region, followed by Nur Leena Wong from Universiti Putra Malaysia (54 new species). The results suggested that reporting of new species from MYB is increasing, which may reflect the many unexplored sites and suggests the existence of as yet unrecorded species in this ecoregion. In this regard further expeditions and research should improve estimates of the true number of aquatic gastropod species in this area. These results can help the regional governments in making a regional biodiversity database as well as in taking decisions regarding aquatic gastropod research and management.
A ray of hope in the darkness: What we have learned from Yangtze giant soft-s...AbdullaAlAsif1
The Swinhoe's softshell turtle, Rafetus swinhoei (Gray, 1873),) is one of the world's largest freshwater turtles, and possibly the most endangered turtle species on the planet (Stanford et al., 2018). It has an overall length of over 100 cm and a width of up to 70 cm, and it can easily weigh up to 70–100 kg, maximum weight was recorded at 169 kg (Solimine, 2013; Trong, 2018). Despite its enormous size and unusual look, this species is incredibly secretive and only comes to the surface to breathe, preferring to remain submerged deep down. For this species, there is very little ecological information, and the remaining distribution is unclear. This could explain why it's so difficult to positively identify and confirm occurrences of this species in the wild (Trong, 2018). If we look back to the history and biogeography of this species, it can be found that the existential records were documented in the historical literature of the Chinese and Vietnamese dynasties. This species was once thought to only live along the Red River in China and Vietnam, as well as the lower Yangtze River floodplain in China, but its current population size is estimated to be just one wild individual of undetermined sex and a solitary captivity male in Suzhou Zoo, China. Although recent thorough searches in Yunnan, China, and Vietnam failed to confirm the presence of more wild specimens, some sightings were reported until around a decade ago (Stanford et al., 2018), giving hope that more individuals may yet exist in Vietnam.
Snapper shrimp is a symbiotic organism usually hidden under the rocks, sponges and pen shells in the seagrass and coral habitats. The relationship study within snapper shrimp and pen shell was conducted from Merambong shoal, one of the biggest seagrass beds in peninsular Malaysia. A total of 40 individual pen shells were collected randomly and four species of pen shells were identified. 40 Anchistus custoides were found inhabiting symbiotically in the mantle cavity of the pen shell as solitary males and females and heterosexual pairs. Pen shell, Pinna bicolour and Atrina vexillum recorded the highest average SH 217.79±53.15 mm, SV 2.62±1.36 dm3 and SH 164.10-224.78 mm with the SV 1.18±0.43 dm3, respectively compared to the other species. The size of Anchistus custoides ranged from 15.00 to 20.00 mm in length and it was determined to be female due to the presence of eggs in the pleopods. The length of the cephalothorax and its length were highly related (rs=0.563, p≤0.01, N=40) and found wider in females. A little difference in size between the left and right chela in males of identical length was noticed, although the left chela is much bigger than the right. The significant relationship (rs=0.450, p≤0.01, N=40) between the pen shell length and shrimp (male-female) length revealed that the size of the shell is important to be hosted the snapper shrimp in the shell cavity.
Diversity of fisheries in Sarawak, Northwest Borneo: Present status and conse...AbdullaAlAsif1
Information on fisheries resources in East Malaysia is scarce and poorly known. The current review aims to compile a checklist of fisheries resources in the Northwest Borneo (Sarawak, Malaysia) from available published literature, address the resources' present status, and suggest future monitoring needs for selected critical species. The study also aims to determine several Sarawak land-use issues that are in flux and responsible for habitat degradation. Five hundred sixty-four species belonging to 123 families and 32 orders were recorded from Sarawak waters. Freshwater fish species comprised 48.0% of the total, followed by a marine (36.6%), marine-euryhaline (12.9%), and brackish water (2.5%) species. Of this, Cyprinidae was the most dominant group accounting for the greatest number of species (82 species), followed by Balitoridae (34 species), Bagridae (21 species) and Penaeidae (21 species). Therefore, available fisheries resources should be managed carefully as 48 species (9.0%) are currently vulnerable to extinction. Furthermore, the presence of 20 alien species in Sarawak water bodies also requires attention from the authorities due to the potential disruption of aquatic ecological balance. Changing land use issues in Sarawak such as forest degradation, agricultural expansion, peatland deforestation and conversion, logging, destruction of mangrove forests, and construction of hydroelectric power dams and flood mitigation channels pose significant challenges to fishery management in Sarawak. Our study documents the priority of fishery monitoring and conservation in Sarawak water bodies to ensure sustainable management of fisheries resources.
Availability and potentiality of small indigenous species of fish throughout ...AbdullaAlAsif1
This study was conducted with aiming to know the present status of small indigenous fish species (SIS) in Oxbow Lakes/baors and rivers as well as the availability of SIS of fish and their potentiality in the study area. Regular data were collected from some important fish landing center cum markets, Oxbow Lakes and rivers of the study area. The availability of small indigenous species (SIS) of fish declined to a great extent over the years and many of them are rare or endangered due to death of rivers and baors were brought under aquaculture. Besides these, Many of SIS of fish were being increasingly used as trash fish for fish feed purpose. As an over populated country, demand of SIS of fish is increasing day by day. After 1990s almost all Oxbow Lakes have been gradually trying to bring under aquaculture through local community or Department of Fisheries. The most significant message of this study is that, some vulnerable and endangered SIS of fish are locally abundant both in fish landing center, baors and rivers. So they must be conserved for their bioavailability and nutrition throughout the country that would be most intelligent efforts in favor of global ichthyo-biodiversity conservation.
Socio-economic conditions of the fish farmers in Jessore, BangladeshAbdullaAlAsif1
The study was conducted the fish farmers in some selected area at sadar upazila in Jessore under the district of Jessore from September to December 2013. A total of 100 fishermen were selected by stratified random sampling method and interviewed. The average me mbers of single family were 5-6 people per household. Single families were much more (62 %) than joint families. Most of the fish far mers were Muslims 92% and few of them were Hindus 8%Most of them had the secondary educational background and some of them with different levels of higher education. It was found that the number of school going children was 2-3 per house. Full katcha (17 %) houses were few, while the semi-pucca (40%) and pucca (43%) houses were more abundant. Regarding health and sanitation, 31% fishermen reported to suffer fro m gastric and 17% suffered from fever. Most of the interviewees were found to take credit facilities from different sources for the subsistence of their family and their venture. The 98% were found to have their tube-wells, others using Governmental tube-well, or those belonging to schools or neighbors However, they need more institutional, organizational, and technical and credit support for their better socioeconomic and sustainable livelihood.
Socio-economic condition of fish fry and fingerling traders in greater Jessor...AbdullaAlAsif1
The present study was conducted to assess the livelihood status of fish fry and fingerling traders and socioeconomic condition in the greater Jessore, Bangladesh from April 2013 to September 2013. Data were collected through the use of well-structured questionnaire from the selected area. 73% of traders have single family but only 27% have joint family. 45% fish fry and fingerling traders family has four members. 20% family has five members, 14% family has six members, 12% family has three members 9%family has seven to eight members respectively. 92% traders has own bank account but only 7% traders uses their bank account when they trade fry and fingerling. 87% traders have secondary business like rickshaw business, fruit business, cloth business, fish trading etc. Among 300 fish fry and fingerling traders the sanitation facilities are present 100%. It was found that 69% and 31% of fish farmers used semi-pucca and pucca toilet respectively. Among 300 fish fry and fingerling traders 78% of traders have own house but only 22% of trader live in rental house or place. 46% of traders have institutional education that range from primary to higher education and other 54% of traders have no experience in education. In case of illness 58% of traders and trader's family goes to government hospital, 24% uses private clinic, 15% traders are uses local village doctor and another 3% does not get any treatment or not require no treatment. 93% traders have television, 5% house consist radio and another 2% house no instrument for entertainment. Only 10% trader wants their son and grandson engages with this fish fry and fingerling trading profession. The average monthly income of traders in the peak season is 18000 taka and off-peak season the income is less than 7000 taka per month.
Marketing of fish and fishery products in Dinajpur and livelihoods of the fis...AbdullaAlAsif1
Marketing of fish and fishery products in Dinajpur and livelihoods of the fish retailers were studied during the period from January to May, 2013. A total of 60 fish retailers were interviewed for obtaining pertinent data. A survey questionnaire was developed, pre-tested and finally used for collection of data. The collected data were tabulated using conventional statistical tools. The result showed that the livelihoods of a large number of people are associated with fish and fishery products distribution and marketing in six different markets. Three types of marketing channel were found to be operated in the selected markets. The shorter marketing chain which included the fish farmers, retailers and consumers was found to be more beneficial to the fish producers. Fish traders in village markets generally operate a capital of around TK. 8,000-10,000 and traders in town markets a capital of around TK. 10,000-20,000 per day. It was found that most of the fish traders used their own money. Price of fish depends on market structure, species, and freshness, supply demand of fish and size of fishes. There are seasonal variation in prices with the highest in summer (March to May), and lowest in pre-winter: (September to November) during the fish harvesting season. Different types of dried and salted fish were sold in the markets. Traders have broadly improved their food consumption facilities, standard of living, and purchasing power as an economic sector. However, the unhygienic conditions of the marketplace, lack of ice facilities, poor infrastructure, inadequate storage and poor transportation facilities were reported to be the major constraints hindering the marketing system in the surveyed areas. It is therefore necessary to provision of governmental, institutional and banking assistance for improvement of the marketing system.
Socio economic status of fry collectors at Sundarban regionAbdullaAlAsif1
The study was conducted on the livelihood approach of fry collectors at Shyamnagar upazila under Satkhira district. The objective of the study was to assess the present livelihood status, to identify the socio-economic condition and problem associated with the alternative livelihood approach of fry collectors. The survey was conducted on three unions of Shyamnagar upazila adjacent to Sundarban as study area. In the study area average family size was 5 persons per family. The maximum age level was 20-30 years old. Among the fry collectors 66% was male and 34% was female. Among them 62% was Muslim and 38% was Hindu. About 78% of total fry collectors are landless in the study area. Literacy was 31% of them where female literacy was 28% and male literacy was 72% and most of them can sign only. Most of the collectors about 63% lived in mud walled with golpata shed house. About 73% families have two earner and 68% have single family. Peak season of fry collection was March to May. For collecting fry they use push net, set bag net mainly. They collect fry in the early morning and in early evening for about 6-8 hours. Men collect 150-200 pieces of fry per day in peak season and 40-60 pieces of fry by women. Price varies from 800-3500 Tk. per thousand golda fry. Price is higher in the month on March-May. About 52% collectors engaged in fishing during off season as alternative income source. Their preferable alternative income source are sustainable agriculture, fry nursing, poultry farming, planned fish cultivation, cattle and goat rearing etc. The main problem of converting of fry collection to other profession was lack of other training of other works, lack of capital, poor infrastructure.facilities, absence of industrialization, lack of land, government and NGO level work is very limited.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
2. Proximate and Mineral Composition of the Long Spined Sea Urchin (Diadema setosum) Roe
31
2016. Japan appeared to the highest-produced country of sea urchin products with 75% of the total
imports [15].
In Malaysia, previous studies have highlighted the abundance and distribution of sea urchin inhabit
Malaysian waters [16],[17],[18],[19]. Twelve tropical species that have been documented in Malaysia
waters, such as Diadema setosum, D. savignyi, Echinometra mathaei, Astropyga radiata, Toxopneustes
pileolus, Echinothrix calamaris, Echinothrix diadema, Parasalenia gratiosa, Salmacis sphaeroides,
Pseudoboletia maculata, Tripneustes gratilla and Salmaciella dussumieri [19],[20]. The most abundance
sea urchin available in Peninsular Malaysia is D. setosum. Unlike the ecological status, information on
nutritional quality such a protein and trace element composition of D. setosum are still scarce. However,
the nutrient composition of other species such as Stomopneustes variolaris [21],[22], Paracentrotus
lividus [15] and Echinometra vanbrunti [23] have been studied to ensure their suitability for human
consumption. From the health point of view, sea urchin roe has high nutritional value as compared to
other seafoods which consists of mainly, protein, carbohydrates, vitamins and minerals
[24],[25],[26],[27]. In Malaysia, D. setosum is still under-consumed, mainly because it is not part of local
gastronomic culture except in some local villages in Sabah. In contrast, commercial harvesting of this
species is increasing in other countries [28],[29].
The construction of breakwater in Kuala Nerus district created a new habitat with different
characteristics and functions. These new habitats have in turn, increased the population of sea urchin
[10]. The emergence of D. setosum in the breakwater areas offer a new opportunity to be explored in
terms of seafood product. Whilst the suitability of D. setosum for human consumption is still unknown, a
study on the nutrient compositions of this species is imperative before in can be commercialized.
The aim of the present study, therefore, is to determine the proximate and element composition of D.
setosum collected around the breakwater in Kuala Nerus coasts. Findings from this study will provide an
important baseline information on the proximate value of D. setosum. In addition, due to its
characteristics such as sedentary, tolerant to pollution and grazing-feeder [30] the present understanding
the proximate values of D. setosum will provide more insights on the safety of seafood products.
MATERIALS AND METHODOLOGY
Collection and preparation of specimens
The sea urchins were collected around Tok Jembal breakwater area located in Kuala Nerus district,
Terengganu, Malaysia (Figure 1) in October 2019, November 2019 and January 2020. Three individuals of
sea urchins were collected by hand from the same locality at the same low tides in each month, making
altogether 9 individuals of this organism collected. All specimens were transported to the laboratory in 10
L bucket and processed within 4 h of collection. Sea urchin roe, the important part for human
consumption was used for all analyses. The shells were opened, and gonads removed. The weight of the
roe was recorded using an analytical balance with precision of up to 0.001 g (And, HR-250AZ). Individual
roe was divided into four pieces for proximate (moisture and ash, lipid and protein) and trace elements
analysis.
Proximate analysis
Moisture content analysis
Moisture content was calculated based on the percentage of weight loss after drying to a constant weight
at 60ºC for a minimum 48 h in a heat oven (Memmert, UFB500). To determine the ash content, the dry
samples were weighed and transferred to a muffle furnace (Carbolite, ELF1123) at 600ºC for 24 h and the
ash content was calculated as a percentage value [31].
3. Rahman, et al. │ Borneo Journal of Sciences & Technology | Vol. 05, Issue 01 (2023)
32
Figure 1. Map of the sample collection in Tok Jembal breakwater.
Protein analysis
Composite samples of urchin used to analyze protein content by taking 0.5 g of homogenized samples
were taken. The protein content was determined according to the [31]. The process started by, taking the
hydrolyzed sample with 5 ml concentrated sulphuric acid (h2so4) and adding one tablet of kjedahl in the
micro kjedahl tube at 420ºc for 1 h. After cooling, h2o was added to the hydrolysates before
neutralization and titration. The amount of total nitrogen in the raw material was multiplied with a
traditional conversion factor of 6.25 to determine the e total protein content. Protein content was
expressed as a % of the dry sample weight.
Lipid content
Lipids were extracted using a Soxtec apparatus, as described by [32]. Samples were heated with 50 ml
petroleum ether at 90ºC for 1 hour. The extracted lipid was removed from the apparatus at 100ºC in the
oven. The lipid content was expressed as a % of the dry sample weight.
Analysis of mineral composition
For mineral analysis, 0.5 g samples of urchin-hins roe were dissolved in HNO3 and subjected to acid
digestion procedure US EPA Method, 1994 [33]. Mineral concentrations were analysed by inductively
coupled plasma mass spectrometry (ICP-MS).
Statistical analysis
One-way ANOVA (analysis of variance) was performed using the SAS 9.4 software for Windows (version
9.4; SAS Institute Inc., 2012, Cary, NC, USA) [34], while tukey-test was performed to determine the mean
comparison and significance level among different sampling time. A multivariate analysis by mean of
Principal component analysis (PCA) was performed on mineral compositions of sea urchin roe. This
ordination was measured usingthe Euclidean distance matrix (dissimilarity) to determine the similarity
between months. All the multivariate analysis was performed using PRIMER v6 [35].
4. Proximate and Mineral Composition of the Long Spined Sea Urchin (Diadema setosum) Roe
33
RESULTS AND DISCUSSION
The ash content was found significantly (p < 0.0001) higher in October (10.89±0.18%), followed by
December (5.72±0.14%), and January (5.37±0.25%) (Figure 2). In contrast, no significant difference was
observed in moisture content during sampling periods. Meanwhile, the lipid content was significantly (p
= 0.0007) higher in October (26.18±1.24%), followed by January (20.87±1.02%), and December
(20.17±0.80%) (Figure 2). The protein content of sea urchin roe was found significantly (p = 0.0016)
higher in January (50.14±4.63 %), followed by October (38.60±0.61%), and December (36.21±0.44%)
(Figure 2).
Figure 2. Proximate composition of sea urchin roe for three different months. Data expressed in mean ±
standard error
The zinc (Zn) content of sea urchin roe was significantly (p = 0.0048) higher in December (57.27±5.85
µg/Kg), followed by October (39.83±5.84 µg/Kg), and January (30.53±6.60 µg/Kg). The cobalt (Co)
content was significantly (p = 0.0037) higher in October (0.13±0.01 µg/Kg), followed by December
(0.09±0.02 µg/Kg), and January (0.06±0.00 µg/Kg). The selenium content of sea urchin roe was also
temporally significantly different (p = 0.0027) with the highest recorded in December (1.98±0.22 µg/Kg),
followed by October (1.38±0.22 µg/Kg) and January (0.69±0.33 µg/Kg). The highest manganese content
was recorded in October with 11.37±2.47 µg/Kg, which was significantly higher (p = 0.0027) than in
January (4.72±0.84 µg/Kg) and December (3.86±1.19 µg/Kg). October also recorded the highest
cadmium (Cd) content with 0.23±0.05 µg/Kg. This was significantly higher (p = 0.0018) than in
December (0.13±0.03 µg/Kg), and January (0.05±0.01 µg/Kg) (Table 1). Meanwhile, no significant
difference between months was recorded for calcium, iron, copper, nickel, lead, and aluminum
5. Rahman, et al. │ Borneo Journal of Sciences & Technology | Vol. 05, Issue 01 (2023)
34
concentration. The differencein mineral composition between months is graphically presented in the
Principal Component Analysis (PCA) (Figure 3).
Table 1. Mineral composition of the sea urchin roe in three different months
Elements (µg/Kg) October December January p-value
Ca 538.00±185.07 784.00±758.72 401.00±136.96 0.6091
Fe 158.67±36.25 114.63±42.11 112.37±15.48 0.2377
Zn 39.83±5.84 57.27±5.85 30.53±6.60 0.0048*
Cu 184.57±157.23 55.00±77.20 15.59±12.14 0.1833
Co 0.13±0.01 0.09±0.02 0.06±0.00 0.0037*
Se 1.38±0.22 1.98±0.22 0.69±0.33 0.0027*
Mn 11.37±2.47 3.86±1.19 4.72±0.84 0.0027*
Ni 1.25±0.25 0.82±0.06 0.90±0.30 0.1108
Pb 0.33±0.08 0.29±0.14 0.18±0.06 0.2367
Al 48.37±7.83 43.47±13.50 87.53±91.38 0.5735
Cd 0.23±0.05 0.13±0.03 0.05±0.01 0.0018*
*Indicate significant difference p < 0.05; all data presented based on fresh weight. Values are mean value and ± SD:
n, the number of samples.
Figure 3. A PCA ordination plot of the difference between months according to the trace element composition.
The difference is based on the Euclidean distance
6. Proximate and Mineral Composition of the Long Spined Sea Urchin (Diadema setosum) Roe
35
The PCA shows that all samples were different in terms of trace elements composition. This is
evidenced by the clear cluster of samples according to months. However, the influence of each element on
contributing to the difference is somewhat low, judging from the short vector lines, except for Zn and Se.
Samples in October are separated from December largely on PC2, in the direction of increasing Mn, Ni,
Cu, Fe, Cd and Pb, while decreasing levels of Zn, Se, Ca, and Pb, where these elements were recorded
higher in December to October. The difference in C2 is also shown between samples in December and
January. December recorded a higher content of Zn, Se, Ca and Pb while the only element with higher
proportion recorded in January was Al. This element (Al) also determined the difference between October
and January (as seen on PC1). The distribution of samples based on trace elements composition was also
influenced by the weight of the sea urchins (Figure 4). Samples that were influenced by Zn, Se, Ca and
Palso corresponded to the higher weight.
Figure 4. A PCA ordination plot of the trace elements composition with super-imposed samples weight of each
sample
DISCUSSION
Evaluation of the proximate analysis of the sea urchin roe indicates that this species has high protein,
comparable to other commercial sea urchins from other parts of the world. This study also provides the
trace element composition available in the sea urchin roe. The main outcomes of this study are consistent
with h previous study on sea urchin showing high protein [21],[36], with low lipid content [25],[37],[38]
and, rich in essential elements such as Fe and Zn [39],[40].
The ash content of D. setosum ranged between 5.3% and 10.9% in the current study was also in
accordance to the previous studies on the sea urchin roe. [41], found 8% of ash in gonadal parts of D.
setosum collected in the northeast Kenya. However, a lower range was recorded in the offshore waters on
off Nagasaki ranging from 1.8 to 2.6 % [42]. Study on other species of sea urchin by [21] reported the ash
content of Stomopneustes variolaris might reach up to 3.76% ± 0.25% in Indian waters. The moisture
content of D. setosum roe ranged between 14.7 and 18.2% in the present study was consistent with the
study of Kaneko36 where the ash content varied between 63.9 and 76.4%. Meanwhile different species
Stomopneustes variolaris contained 69.3% [25] and 77.5% ± 0.80% of moisture in gonad [21].
Nevertheless, the food quality available in the environment could influence the proximate composition of
the urchins [43].
7. Rahman, et al. │ Borneo Journal of Sciences & Technology | Vol. 05, Issue 01 (2023)
36
Protein plays a vital role as it serves as an energy supply, particularly during reproductive activity [26].
The protein content of the D. setosum (ranged between 36.2% and 50.1%) in the present study was on the
higher end of the range recorded in previously studied sea urchins [36] found 187.5 mg/g of protein in D.
setosum from Hong Kong coast, whereas [42] reported the gonadal protein of the D. setosum might be
ranged between 14.1 and 17.0% from Nagasaki, Japan coast. The other species of sea urchin,
Stomopneustes variolaris recorded an average of 12.1% of the protein content from the Indian water [21].
Protein is the main constituent of the sea urchin roe which possibly can be as an alternative’s sources
instead of other seafood supplements.
After protein, lipid was the next dominant organic component of the urchins. Lipid content of D.
setosum ranged between 20.2% and 26.2% in the present study. This supported by previous study on the
lipid content of D. setosum collected from central Vietnam was found to be 25.0% [44]. On the other
hand, previous research observed a lower lipid content on the urchins available in Nagasaki and Kenya
[42] and Kenya [41]. Overall, D. setosum in the present study recorded a comparable content of lipid to
the other sea urchin species such as Paracentrotus lividus, S. variolaris and Evechinus chloroticus
[21],[25],[37],[38]. However, by comparison the lipid content in the present study was lower than protein,
which was in contrast with study by [36] and [40] where the lipid was consistently higher in composition
compared to the protein.
In general, mineral compositions of D. setosum in the present study were within the recommended
daily intake [45] (Table 2), except for Zn which recorded a much higher range. The possible explanation is
that the Zn content is highly fluctuate especially among samples taken at different time or environmental
conditions [39],[40]. Most of minerals contained in D. setosum in the present study were also
comparable to previous studies [39][25][40]. In addition, the findings from the present study were also
consistent with previous studies on other sea urchin species such as P. lividus, S. variolaris and E.
chloroticus [15],[21],[30],[46].
As for the multivariate perspective on mineral compositions, temporal variation seemed to affect the
mineral compositions, and this is conformed to the findings in the previous study [40]. The variation was
fairly apparent for Al where this mineral was highly corresponded to January while other minerals were
more associated to October and December.
This contrast composition might be due to the uptake of aluminium in aquatic organisms essentially
increases when the water hardness decreasing, such as in the condition low dissolve minerals in the water
[47],[48]. The aluminium composition in the present study was generally higher when the other minerals
were lower, and vice versa when the other minerals were higher. Similarly, [49] also found out that in the
presence of zinc and copper, the aluminum content in flagfish Jordanella flowidae was generally low.
Table 2. Recommended Daily Intake (RDI) and Tolerable Upper Intake (TUI) of nutrients based on Malaysian
standard [45]
Elements Recommended Daily Intake Tolerable Upper Intake
Calcium (Ca) 1000 mg 2500 mg
Magnesium (Mg) 320 mg 350 mg
Iron (Fe) 1.3 – 2.94 mg 4.7 – 5.5 mg
Zinc (Zn) 4.3 – 6.2 mg 35 mg
Copper (Cu) 900 µg 1000 µg
Manganese (Mn) 1.8 – 2.3 mg 11 mg
Selenium (Se) 24 µg 400 µg
Cadmium (Cd) 1 µg NA
Lead (Pb) 3.57 µg NA
8. Proximate and Mineral Composition of the Long Spined Sea Urchin (Diadema setosum) Roe
37
CONCLUSION
This study revealed the high proximate and mineral compositions of sea urchin D. setosum roe, which
can be considered as one the major nutritional sources. This study also found the presence of heavy
metals in the D. setosum roe, which suggest that environment is moderately polluted and D. setosum can
be used as the bio-indicator for marine habitats. To better understand the environmental variation and
its effects on the mineral uptake by organisms, it is recommended that this type of study be expanded for
a longer spatial scale across different monsoon seasons.
ACKNOWLEDGEMENT
I would like to thank Associated Professor Dr Abu Hena Mustafa Kamal for reviewing the manuscript.
Also, the authors would like to thank Mr Zafrul Abu Bakar, Mr Mohamad Tarmizi Bin Abdullah and Mr
Shahrul Ali for their assistance.
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