The document discusses methods for measuring habitat quality, including chemical analysis of water to detect contaminants and biotic analysis to evaluate species richness, abundance, and water quality. It notes that some species are more tolerant of poor water quality while others require high quality. These bioindicator species can reveal the quality of the environment. The document also describes using amphibians as bioindicators through an index that accounts for their permeable skin and sensitivity in both aquatic larval and terrestrial adult stages. It lists species of amphibians that can be found at the Fernald Preserve site.