Arthropod vectors of pathogens cause enormous economic losses and are a fundamental challenge for sustainable increases in food production, yet agricultural pathosystems remain an underserved area of research. To more effectively fight plant diseases, data pertaining to a disease system needs to be consolidated, made searchable and amenable to data mining. The AgriVectors platform is an open access and comprehensive resource for growers, researchers and industry working on plant pathogens and pathosystems spread by arthropod vectors. The portal connects established public repositories with pathosystem-specific data repositories. The AgriVectors system will provide tools to enable technologies such as RNAi, CRISPR, screening bioassays, etc. to leverage current and emerging knowledge across disciplines. It will also include private and unpublished data, using passwords and secure protocols for restricted access. The portal will be based on the Citrusgreening.org (https://citrusgreening.org/) community resource that was developed as a model for systems biology of tritrophic disease complexes. Citrusgreening.org provides omics and biology resources for the Huanglongbing pathosystem. In addition, it includes a biochemical pathway database for each organism in this disease complex, and an expression atlas with proteomics and RNAseq data from psyllids (http://pen.citrusgreening.org) and citrus (http://cen.citrusgreening.org) across multiple infection states. The AgriVectors portal will extend this model beyond gene-centric omics data to the broader Pathosystem-wide information, with integrated pest management, behavioral, plant health, soil health and climate data to incorporate rapid phenotyping information from research trials, building a foundation for more effectively identifying solutions to combat plant diseases.

1. www.agrivectors.orgwww.agrivectors.org
AgriVectors.org
A Systems Biology resource for
Vector Biologists
Data to Disease Management
Surya Saha
Boyce Thompson Institute, Ithaca, New York, USA
ss2489@cornell.edu | @SahaSurya
Agricultural Vectors Consortium

2. www.agrivectors.orgwww.agrivectors.org
Mueller Lab @ Boyce Thompson Institute
Open source toolkit

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Project Workflow
Mueller Lab

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Genome Annotation
Target for interdiction molecules
Pathway Databases
Expression Networks
…….
Omics resources and databases are required for identification of targets for interdiction
Vector Host
Pathogen

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DiaphorinaCyc Pathways overlaid with Gut RNAseq results

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Psyllid Expression Network (PEN)
Colored by
level of
expression
Genes correlated with
DcitrG075700
tyrosine protein
kinase Fps85D-RA
Mirella Flores
Mueller Lab
Gut RNAseq (Citron)
Male and female abdomen (C.
reticulata)
Male and female antennae (C.
reticulata)
Whole body (C. macrophylla)
Whole body (Citrus spp.)

8. www.agrivectors.orgwww.agrivectors.org
Outreach
• News
• Events
• Blog
• Publications

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Portal for all Agricultural Disease
Vector Systems
Citrusgreening
Zebra chip
Pierce’s disease
Pathogens: Bacteria, virus and fungi
AgriVectors Home Page
Andrew Jensen
Alex Wild

10. www.agrivectors.org
AgriVectors
Knowledge Base
Data Producers Data Consumers
Public
Repository
(remote)
Pathosystem
Repository
(local and remote)
Topic
Repository
(local and remote)
Researchers
Extension agents
Industry
INRA
USDA ARS / APHIS
CRISPR / RNAi genes
Bacterial effectors
Microbiome
Geospatial disease data
Your Pathosystem
Zebrachip
Citrusgreening
Pierce’s disease
Researchers
INRA / USDA ARS / APHIS
IPM product development
Outreach and extension
Educators
Secure portal
Patents
Commercial
AgriVectors
Data Schema
NCBI / EMBL / DDBJ
Ag Data Commons
i5k

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Omics data sets
• Genome assembly and official annotation
• Transcriptomes
• Mass spectrometry proteomics
Population biology
• Insecticide resistance
• SNPs and Indels
• Variants
• Simple Sequence Repeats
• Mitochondrial genes
Data types

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Data types
Integrated pest management pathosystem-wide data
• Inclusive of Vector, pathogen, host, environment and
beyond...
• Gene family based data sets (P450, RNAi pathway)
• E.g. Virus, Bacteria, or fungal infection assays
• Electrical Penetration Graph (EPG) feeding data
• Phenotyping data from disease trials
• Ecological and climactic data
• Behavioral assays
• Toxicology, Insecticide resistance, etc.
Genes (structural and functional curation)
Publications, notes, posters, videos and abstracts…………

13. www.agrivectors.orgwww.agrivectors.org
Project ideas and Preliminary data
Salivary gland transcriptomics of hemipterans
• Host range based on orthologous enzymes and proteins
• Pathogen acquisition/transmission/…replication?
• Chemical and pathogen response
Host immunity based on pathogen exposure
Fecundity and development assays
Microbiome and virome characterization

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AgriVectors Roadmap
2018
• Build research, government and industry partnerships
• Design Citrusgreening.org as a model system
• Generate preliminary data sets
2019
• Establish primary and secondary consortia members
• Present to community at scientific meetings
• Fundraising for sub-projects
• Integrate and mine novel data types at Citrusgreening.org
2020+
• Fundraising for AgriVectors umbrella project
• Apply for research coordination network (RCN) funding for AgriVectors community
meetings

15. www.agrivectors.orgwww.agrivectors.org
Agricultural Vectors Consortium
United States Department of Agriculture
Wayne Hunter (Ft. Pierce, FL)
Michelle Heck (Ithaca, NY)
Rodney Cooper (Wapato, WA)
Lucy R. Stewart (Wooster, OH)
University of Idaho
Shirley Luckhart
Edwin Lewis
Sanford Eigenbrode
Washington State University
Daisy Fu
Texas A & M University
Cecilia Tamborindeguy
Oklahoma State University
Astri Wayadande
University of Florida
Jawwad Qureshi
You??

16. www.agrivectors.orgwww.agrivectors.org
@Citrusgreening // @SahaSurya
Thank you!!
Poster PO0237

17. www.agrivectors.orgwww.agrivectors.org
AgriVectors
Knowledge Base
Data Producers Data Consumers
Public
Repository
(remote)
Pathosystem
Repository
(local and remote)
Topic
Repository
(local and remote)
Researchers
Extension agents
Industry
INRA
USDA ARS / APHIS
CRISPR / RNAi genes
Bacterial effectors
Microbiome
Geospatial disease data
Zebrachip
Citrusgreening
Pierce’s disease
Researchers
INRA / USDA ARS / APHIS
IPM product development
Outreach and extension
Educators
Secure portal
Patents
Commercial
AgriVectors
Data Schema
NCBI / EMBL / DDBJ
Ag Data Commons
i5k
Improved Rapid Data Cluster Analyses
and Access