Ag Ab I (Dwd)

Antigen - Antibody Reactions DWD

Antigen – Antibody Reactions ,[object Object],[object Object],[object Object]

Uses of Antigen – Antibody Reactions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Nature of Ag/Ab Reactions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Stages of Antigen – Antibody Reactions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Secondary Stage ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Secondary Stage contd . --------- ,[object Object],[object Object],[object Object],[object Object]

Role of Immunoglobulin Classes in different Serological Reactions. Negative Strong Weak Lysis Negative Weak Strong Complement fixation Moderate Strong Weak Agglutination Variable Weak Strong Precipitation IgA IgM IgG Immunoglobulin Class Serological Reactions

Antigen – Antibody reactions ,[object Object],[object Object],[object Object],[object Object]

Characteristics of Ag – Ab Reaction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Affinity ,[object Object],[object Object],[object Object],[object Object]

Measurement of Ag & Ab ,[object Object],[object Object],[object Object],[object Object],[object Object]

Cross Reactivity ,[object Object],[object Object]

Factors Affecting Measurement of Ag/Ab Reactions ,[object Object],[object Object],[object Object],[object Object]

Tests Based on Ag/Ab Reactions ,[object Object],[object Object]

Precipitation Tests ,[object Object],Equivalence – Lattice formation

Precipitation Reaction ,[object Object],[object Object],[object Object]

Applications ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Immunodiffusion ( Precipitation in Gel) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Immunodiffusion ‘ Oudin procedure’ ‘ Oakley – Fulthorpe procedure

Radial Immunodiffusion (Mancini) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Oucheterlony procedure e.g. : Elek’s test for toxigenicity in C. diphtheriae

Countercurrent electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Ag Ab - +

Rocket Electrophoresis ,[object Object],[object Object]

Laurell’s two dimensional electrophoresis ,[object Object],[object Object],[object Object],[object Object]

Agglutination Reactions ,[object Object],Equivalence – Lattice formation

Agglutination Reactions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Applications ,[object Object],[object Object],[object Object],[object Object],[object Object],Y Y Y + ↔

Applications ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Coombs (Antiglobulin)Tests ,[object Object],[object Object],[object Object],+ ↔ Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Patient’s RBCs Coombs Reagent (Antiglobulin)

Coombs (Antiglobulin)Tests ,[object Object],[object Object],Detection of Anti Rh Ab & Autoimmune hemolytic anemia. Y Y Y Y Y Patient’s Serum Target RBCs + ↔ Step 1 + ↔ Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Coombs Reagent (Antiglobulin ) Step 2

Passive Agglutination Test ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Passive Agglutination Test ,[object Object],[object Object],[object Object],[object Object]

Passive Agglutination Test ,[object Object],[object Object],[object Object]

THANKS That’s all for Now! Stay tuned for more exciting topics on immunology!

Ag Ab I (Dwd)

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