This document summarizes a study that investigated co-culturing endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) to generate vascularized tissue engineered grafts. In monolayer cultures, the co-cultures generated networks through endothelial cell aggregation and sprouting, similarly to physiological blood vessel formation. MSC-conditioned media alone did not elicit this response, indicating direct cell contact is needed. The co-cultures were able to form spheroids containing established endothelial networks that were maintained for up to 21 days. When loaded into scaffolds and cultured in a bioreactor, the spheroids showed outgrowth and presence of pre-vascularized networks, unlike direct cell seeding
Development of cancer therapeutics is often carried out in 2D cultures prior to testing on animal model. In comparison to 2D cultures, discuss the potential of using 3D in vitro models for drug efficiency testing.
Genes and Tissue Culture Technology Assignment (G6)Rohini Krishnan
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant.
Development of cancer therapeutics is often carried out in 2D cultures prior to testing on animal model. In comparison to 2D cultures, discuss the potential of using 3D in vitro models for drug efficiency testing.
Genes and Tissue Culture Technology Assignment (G6)Rohini Krishnan
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant.
3D tumor spheroid models for in vitro therapeutic screening: a systematic app...Arun kumar
The potential of a spheroid tumor model composed of cells in different proliferative and metabolic
states for the development of new anticancer strategies has been amply demonstrated. However, there
is little or no information in the literature on the problems of reproducibility of data originating from
experiments using 3D models. Our analyses, carried out using a novel open source software capable of
performing an automatic image analysis of 3D tumor colonies, showed that a number of morphology
parameters affect the response of large spheroids to treatment. In particular, we found that both
spheroid volume and shape may be a source of variability. We also compared some commercially
available viability assays specifically designed for 3D models. In conclusion, our data indicate the need
for a pre-selection of tumor spheroids of homogeneous volume and shape to reduce data variability to
a minimum before use in a cytotoxicity test. In addition, we identified and validated a cytotoxicity test
capable of providing meaningful data on the damage induced in large tumor spheroids of up to diameter
in 650 μm by different kinds of treatments.
Cancer stem cell theory and evidence from colorecatalKareem Ahmed
This is a presentation of a review article explaining theory of cancer stem cells with evidences from colorectal cancer at a glance. It was presented at Student Research Symposium at Faculty OF Medicine, Assiut University, Assiut, Egypt,
Cytes Biotechnologies S.L. offers in its portfolio an assortment of various Human Endothelial Cells for research. These hepatic non-parenchymal liver cells are offered both fresh and cryopreserved to better meet the customer’s specific needs. All products are fully characterized by phenotypic markers, induction, metabolism, 3D, and transporters.
Building on the sell-out success of the launch event, SMi Group is delighted to announce the return of 3D Cell Culture, taking place on 21st and 22nd of February 2018, in London UK.
3D Cell Culture is rapidly growing with incredible potential for industrial application and a widespread reach that can be seen across many different fields, such as 3D bioprinting and microfluidics.
The 2nd annual conference will explore these overlapping areas and will combine pioneering breakthroughs with scientific research to strengthen your commercial success. Join us for exclusive insight into key topics such as disease models, organoids, organ-on-a-chip technologies, Ipsc advances and CRISPR technology. Notable speakers on the agenda for 2018 will include experts from Aurelia Bioscience, ReInnervate Ltd, Cell and Gene Therapy Catapult, University College London, Novartis Institutes for Biomedical Research, Kugelmeiers, GSK, AstraZeneca, Roche and more!
Extracellular molecules, such as nucleotides, lipids, short peptides or proteins, are released by cells and bind to receptors on the other cells, which are important mediators in cell-to-cell communications in multicellular organisms. In addition to single molecules, eukaryotic cells can also release membrane vesicles into extracellular environment, such as microvesicles, apoptotic blebs and exosomes.
Maria A. Diroma – MEWAs: sviluppo di un sistema bioinformatico per studi di a...eventi-ITBbari
MEWAs (Mitochondriome-Exome Wide Associations): sviluppo di un sistema bioinformatico per studi di associazione fra l’intero esoma nucleare e il DNA mitocondriale in fenotipi fisiologici o patologici.
3D tumor spheroid models for in vitro therapeutic screening: a systematic app...Arun kumar
The potential of a spheroid tumor model composed of cells in different proliferative and metabolic
states for the development of new anticancer strategies has been amply demonstrated. However, there
is little or no information in the literature on the problems of reproducibility of data originating from
experiments using 3D models. Our analyses, carried out using a novel open source software capable of
performing an automatic image analysis of 3D tumor colonies, showed that a number of morphology
parameters affect the response of large spheroids to treatment. In particular, we found that both
spheroid volume and shape may be a source of variability. We also compared some commercially
available viability assays specifically designed for 3D models. In conclusion, our data indicate the need
for a pre-selection of tumor spheroids of homogeneous volume and shape to reduce data variability to
a minimum before use in a cytotoxicity test. In addition, we identified and validated a cytotoxicity test
capable of providing meaningful data on the damage induced in large tumor spheroids of up to diameter
in 650 μm by different kinds of treatments.
Cancer stem cell theory and evidence from colorecatalKareem Ahmed
This is a presentation of a review article explaining theory of cancer stem cells with evidences from colorectal cancer at a glance. It was presented at Student Research Symposium at Faculty OF Medicine, Assiut University, Assiut, Egypt,
Cytes Biotechnologies S.L. offers in its portfolio an assortment of various Human Endothelial Cells for research. These hepatic non-parenchymal liver cells are offered both fresh and cryopreserved to better meet the customer’s specific needs. All products are fully characterized by phenotypic markers, induction, metabolism, 3D, and transporters.
Building on the sell-out success of the launch event, SMi Group is delighted to announce the return of 3D Cell Culture, taking place on 21st and 22nd of February 2018, in London UK.
3D Cell Culture is rapidly growing with incredible potential for industrial application and a widespread reach that can be seen across many different fields, such as 3D bioprinting and microfluidics.
The 2nd annual conference will explore these overlapping areas and will combine pioneering breakthroughs with scientific research to strengthen your commercial success. Join us for exclusive insight into key topics such as disease models, organoids, organ-on-a-chip technologies, Ipsc advances and CRISPR technology. Notable speakers on the agenda for 2018 will include experts from Aurelia Bioscience, ReInnervate Ltd, Cell and Gene Therapy Catapult, University College London, Novartis Institutes for Biomedical Research, Kugelmeiers, GSK, AstraZeneca, Roche and more!
Extracellular molecules, such as nucleotides, lipids, short peptides or proteins, are released by cells and bind to receptors on the other cells, which are important mediators in cell-to-cell communications in multicellular organisms. In addition to single molecules, eukaryotic cells can also release membrane vesicles into extracellular environment, such as microvesicles, apoptotic blebs and exosomes.
Maria A. Diroma – MEWAs: sviluppo di un sistema bioinformatico per studi di a...eventi-ITBbari
MEWAs (Mitochondriome-Exome Wide Associations): sviluppo di un sistema bioinformatico per studi di associazione fra l’intero esoma nucleare e il DNA mitocondriale in fenotipi fisiologici o patologici.
Adipose Tissue and Mesenchymal Stem Cells: State of the Art and Lipogems® Technology Development
Carlo Tremolada1 & Valeria Colombo1 & Carlo Ventura2
Abstract Inthepastfewyears,interestinadiposetissueasan ideal source of mesenchymal stem cells (MSCs) has increased. These cells are multipotent and may differentiate in vitro into several cellular lineages, such as adipocytes, chondrocytes, osteoblasts, and myoblasts. In addition, they secrete many bioactive molecules and thus are considered Bmini-drugstores.^ MSCs are being used increasingly for many clinical applications, such as orthopedic, plastic, and reconstructive surgery. Adipose-derived MSCs are routinely obtained enzymatically from fat lipoaspirate as SVF and/or may undergo prolonged ex vivo expansion, with significant senescence and a decrease in multipotency, leading to unsatisfactory clinicalresults.Moreover, these techniquesare hampered by complex regulatory issues. Therefore, an innovative technique (Lipogems®; Lipogems International SpA, Milan, Italy) was developed to obtain microfragmented adipose tissue with an intact stromal vascular niche and MSCs with a high regenerative capacity. The Lipogems® technology, patented in 2010 and clinically available since 2013, is an easyto-use system designed to harvest, process, and inject refined fat tissue and is characterized by optimal handling ability and a great regenerative potential based on adipose-derived MSCs. In this novel technology, the adipose tissue is washed, emulsified, and rinsed and adipose cluster dimensions gradually are reduced to about 0.3 to 0.8 mm. In the resulting
Lipogems® product, pericytes are retained within an intact stromal vascular niche and are ready to interact with the recipient tissue after transplantation, thereby becoming MSCs and starting the regenerative process. Lipogems® has been used in more than 7000 patients worldwide in aesthetic medicineandsurgery,aswellasinorthopedicandgeneralsurgery, with remarkable and promising results and seemingly no drawbacks. Now, several clinical trials are under way to supporttheinitialencouragingoutcomes.Lipogems®technology is emerging as a valid intraoperative system to obtain an optimal final product that may be used immediately for regenerative purposes.
Stem cell therapy for the bladder has been conducted mainly on an experimental basis in the areas of bladder dysfunction. The therapeutic efficacy of stem cells was originally thought to be derived from their ability to differentiate into various cell types. For more details visit: http://www.cryobanksindia.com/moms-corner/case-studies/
Stem cell therapy for the bladder has been conducted mainly on an experimental basis in the areas of bladder dysfunction. The therapeutic efficacy of stem cells was originally thought to be derived from their ability to differentiate into various cell types. For more details visit: http://www.cryobanksindia.com/moms-corner/case-studies/
Rotator cuff repair using a stem cell approachZakary Bondy
This presentation communicates current methods for rotator cuff repair mainly focusing on mesenchymal and tendon-derived stem cells. It looks to expand on future research in this field by communicating a future experiment to expand on current knowledge of tendon-derived stem cells.
Stem cells and nanotechnology in regenerative medicine and tissue engineeringDr. Sitansu Sekhar Nanda
Alexis Carrel, winner of the Nobel Prize in Physiology or Medicine in 1912 and the father of whole-organ transplant, was the first to develop a successful technique for end to end arteriovenous anastomosis in transplantation.
Genes and Tissue Culture Assignment Presentation (Group 3)Lim Ke Wen
The culture of cells in two dimensions does not reproduce the histological characteristics of a tissue for informative or useful study. Growing cells as three-dimensional (3D) models more analogous to their existence in vivo may be more clinically relevant. Discuss the potential of using three dimensional cell cultures for anti-cancer drug screening.
As a tubularized organ in the distal portion of the urinary tract,
the urethra can often develop strictures due to congenital defects (e.g. hypospadias), injury, and infections. In particular, urethral stricture is a common urological problem in men. Urethral strictures thus present a significant economic impact and burden, because they are relatively frequent and repeated surgical intervention is often needed.
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Presentation Abstract
Title: CO-CULTURES OF ENDOTHELIAL PROGENITOR CELLS AND
MESENCHYMAL STEM CELLS FOR THE GENERATION OF
VASCULARISED TISSUE ENGINEERED GRAFTS
Category: Stem Cells and Tissue Engineering
Secondary
Category:
Endothelial Cells/Hemangioblasts
Presentation
Start:
6/13/2013 6:00:00 PM
Presentation
End:
6/13/2013 8:00:00 PM
Poster
Board
Number:
T-1193
Author
Block
Mark Chong1, Dedy Sandikin1, Renyi Teo2, Wenhao Leow2, Junwei Goh2,
Toon Tien Foo2, Mahesh Choolani1, Jerry K Y Chan3
1Obstetrics and Gynaecology, National Univ of Singapore, Singapore, Singapore,
2Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore,
Singapore, 3Reproductive Medicine, KK Women's and Children's Hospital,
Singapore, Singapore
Abstract: Engineered bone tissues are currently limited by inadequate vascularisation in
vivo following implantation. Recent research has turned to the use of angiogenic
cell sources, including endothelial progenitor cells (EPCs) to generate pre-
vascularised tissue prior to implantation. In this study, co-cultures of umbilical
cord-blood derived EPCs and fetal bone marrow mesenchymal stem cells (MSCs)
were studied for use in the pre-vascularisation of tissue engineered constructs.
Cells were fluorescently-labelled to facilitate imaging and identification in co-
cultures. Culture conditions were then optimised in monolayer cultures, and
subsequently extended to three-dimensional cultures for applications in tissue
engineering. In monolayer cultures, time-lapsed observations demonstrate the
cocultures to generate networks, following endothelial cell aggregation and
angiogenic sprouting, in a process akin to that of physiological vaculogenesis.
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Cultures of EPC in MSC-conditioned media failed to elicit similar results,
suggesting the need for direct cell contact and the stromal/supportive role of MSC
in the system. A modified image analysis method was then developed to
characterise vasculogenic events, and used in the optimisation of culture
conditions to elicit maximal pre-vascularisation. It was established that culture in
complete endothelial growth medium extensive prevascular networks (1.5-fold
increase in tube length over conventional culture conditions, p<0.01). In addition,
MSC were critical for the provision of stromal support, extending vascular
longevity in a dose-dependent manner. Extending these results to three-
dimensional conditions relevant for tissue engineering, the EPC:MSC co-cultures
were induced to form spheroids by culture on non-adhesive plates. Spheroids
measuring 500 um in diameter were generated with well-established endothelial
networks, which were sustained for up to 21 days of culture. Scaling up to
physiologically relevant conditions, co-cultured spheroids were loaded into tissue
engineering scaffolds and maintained in a bioreactor for 3 weeks. Outgrowth of
cells from spheroids onto the scaffolds was observed and pre-vascularised
networks were found to be present, as opposed to direct cell seeding methods. In
conclusion, a method to generate pre-vascularised tissue engineered constructs
was developed and shown to have several promising features. Work in progress
includes evaluation of the constructs in a murine model.
The information about presentations at the ISSCR 11th Annual Meeting is available for
planning purposes only and is strictly embargoed until presentation.
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