The document outlines the maturation process of erythrocytes (red blood cells), detailing the stages from erythroblasts to mature erythrocytes and the time each stage requires. Key characteristics such as nuclear chromatin patterns, n:c ratios, and cytoplasmic colors during maturation are emphasized. It describes the overall decrease in cell and nuclear sizes as well as changes in appearance and timing throughout the maturation stages.

1. Erythrocy
te
Maturatio
n
Here is where your
presentation begins

2. ● RBCs are formally called
erythrocytes.
● Erythroblasts – nucleated
precursors in the BM. AKA
“normoblast” which refers
to developing nucleated
cells (i.e. blasts) with
normal appearance.
Introducti
on

3. ● Megaloblasts – abnormal
appearance of the
developing nucleated cells
because of their large size.
● Megaloblastic anemia - is a
type of anemia
characterized by very large
red blood cells.
Introducti
on

4. ●BFU-E takes about 1 week to mature to the CFU-
E.
●CFU-E takes 1 week to become pronormoblasts.
●Pronormoblasts takes approximately another 6-7
days to become mature enough to enter the
circulation.
●So, approximately 18-21 days are required to
produce a mature RBC from BFU-E.
(Figure 8-1)
Erythroid Progenitors

6. ● The stage of maturation of any blood cell is determined by
careful examination of nucleus and the cytoplasm.
● Important qualities in identification of RBCs are:
a. Nuclear chromatin pattern
b. Nuclear diameter
c. Nucleus : cytoplasm (N:C) ratio
d. Presence or absence of nucleoli
e. Cytoplasmic color
Maturation Process

8. ● As RBCs mature, several general trends affect their
appearance:
1. The overall diameter of the cell decreases.
2. The diameter of the nucleus decreases more rapidly than
does the size of the cell. As a result, N:C ratio also decreases.
3. The nuclear chromatin pattern becomes coarser, clumped,
and condensed.
4. Nucleoli disappear.
Maturation Process

9. Stage
PRONORMOBLAST
NUCLEUS • N:C Ratio – 8:1
• Purpled
Chromatin
CYTOPLASM • Dark blue
LOCATION • Present only in
the BM
LENGTH OF
TIME IN THIS
STAGE
• Lasts slightly
more than 24
hours

10. Stage
BASOPHILIC NORMOBLAST
NUCLEUS • N:C Ratio – 6:1
• Deep purple red
Chromatin
• Condensed
chromatin
CYTOPLASM • Deeper, richer
blue
LOCATION • Present only in
the BM
LENGTH OF
TIME IN THIS
STAGE
• Lasts slightly
more than 24
hours

11. Stage
POLYCHROMATIC
NORMOBLAST
NUCLEUS • N:C Ratio – 4:1
• No nucleoli
present
CYTOPLASM • A mixture of
pink and blue
resulting in a
murky gray-blue
• Slightly pink
means
accumulation of
hgb
LOCATION • Present only in
the BM
LENGTH OF
TIME IN THIS
STAGE
• Lasts approx.
30 hours

12. Stage
ORTHOCHROMIC
NORMOBLAST
NUCLEUS • N:C Ratio – 1:2
• Nucleus is
completely
condensed
CYTOPLASM • Salmon pink
color w/c
reflects nearly
complete hgb
production
LOCATION • Present only in
the BM
LENGTH OF
TIME IN THIS
STAGE
• Lasts approx.
48 hours

13. Stage
RETICULOCYTE
NUCLEUS • No nucleus
CYTOPLASM • Salmon pink
color same as
mature RBCs
• Larger than
mature RBCs
(8-10 um)
• Has irregular
shape
LOCATION • Resides in BM
for 2 days
• Moves in PB for
about 1 day
before maturity
LENGTH OF
TIME IN THIS
STAGE
• 2 days in BM
• 1 day in PB

14. Stage
ERYTHROCYTE
NUCLEUS • No nucleus
CYTOPLASM • Salmon pink
with a central
pale that
corresponds to
concave
• 7-8 um in
diameter
LOCATION • Active in the
circulation for
approx.120
days
LENGTH OF
TIME IN THIS
STAGE
• Apoptosis
• hemolysis

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