This power point will give you a clear cut information about maturation & development of RBC

2. Erythropoiesis
 Maturation & development of RBC is called
Erythropoiesis or Hematopoiesis

3. Site of Erythropoiesis
 Mesoblastic stage
 First 2 months of intrauterine life
 Hepatic stage
 From third month of intra uterine life
 Myeloid stage
 Last three months of intra uterine life

4. Newborn & Adults
 RBC are produced only from Red bone marrow
 In adults liver & spleen will produce the RBC if the
bone marrow is fibrosed
 75% of bone marrow is involved in production of
leukocytes
 25% of bone marrow is involved in production of RBC

5.  Up to age of 20years
 Red bone marrow of all long & flat bones
 After age of 20years
 Membranous bones like
 Sternum
 Scapula
 Vertebra
 From the ends of the long bones

6. Stem Cells
 Primary cells
 Having capability of self renewal
 This can differentiate into many specialized cells

7. UCPHSC
 Un committed pluripotent hematopoietic stem cell
 This can give rise to all the types of cells
 Hence named un committed

8. CPHSC Committed pluripotent hematopoietic stem cell
 Designed to form a particular type of cell
 It will give rise to two groups of cells
 Lymphoid stem cells
 Lymphocytes & Natural killer cells
 Colony forming blastocytes
 Colony forming –E
 Erythrocytes
 Colony froming-M
 Megakaryocytes
 Colony forming –GM
 Neutrophils, Eosinophils, Basophils & monocytes

9. Colony forming unit-E
 Pro-erythroblast
 Early Normoblast
 Intermediate Normoblast
 Late normoblast
 Reticulocyte
 Matured RBC

10. Proerythroblast
 It is also called Megaloblast
 First cell derived from CFU-E
 Bigger in size 20u
 Nucleus is present
 Cytoplasam is basophilic
 Hemoglobin is not present

11. Early Normoblast
 It s is the 2nd cell formed from CFU-E
 Little smaller that Megaloblast
 Size is 15u
 Nuclear membrane disappears
 Nucleus is little bit condensed
 Cytoplasm is basophilic
 Hemoglobin is not present

12. Intermediate Normoblast
 3rd cell derived from CFU-E
 Cell is little smaller when compared with Early
normoblast
 Size is 12u
 Nucleus is condensed
 Hemoglobin starts appearing
 Cytoplasm is basophilic & acidic
 Hence called polychromatic erythroblast

13. Late Normoblast
 4th cell formed from the CFU-E
 Size is 10u
 Nucleus starts disappering
 Quantity if hemoglobin increases
 Cytoplasm becomes more acidic
 Hence called orthochromatic erythroblast

14. Reticulocyte Reticulocyte is also called immatured RBC
 Slightly larger in size when compared with Matured RBC
 Size is 8u
 Cytoplasm contains a remnants if nucleus
 It forms a net like structure hence called reticulocyte
 Cytoplasm is acidic
 2-6 reticulocytes are present in 100-RBC in new born babies
 In adults only-1% of reticulocytes are present
 When the production of RBC is increased count of reticulocytes
increases

15. Matured RBC
 Reticular network disappears
 Size of the cell is 7.2u
 Contains full hemoglobin
 Cytoplasm stains acidic
 Shape is biconcave

16. Important points to remember
 Duration of erythropoiesis is 7 days
 5-days needed for cell to develop up to reticulocyte
 2-days needed to develop into matured RBC

17. Changes occur during
Erythropoiesis
 Cell size decreases
 Cell division stops
 Cytoplasm changes from basic to acidic
 Hemoglobin appears
 Nucleus dsiappears

18. Factors necessary for
Erythropoiesis
 Hypoxia
 Erythropoietin
 Thyroxine
 Vitamins
 Iron
 Folic acid