The effects of cadmium and cow manure on nodulation and growth attributes of ...Agriculture Journal IJOEAR
Abstract— Different biotic and abiotic factors are involved in the availability of heavy metals in soil including organic matter. In order to study the effects of cow manure on cadmium availability and their interactions on common bean nodulation and growth parameters, an experiment was conducted under greenhouse conditions. The treatments included five cadmium levels (0, 2, 5, 10, 20 mg cadmium per kg soil) and four cow manure levels (0, 15, 30, 60 t ha-1) based on a completely randomized design with three replications. Results indicated that increased soil cadmium concentrations caused higher cadmium uptake by root tissues whereas, Root nodulation and total N content of shoot tissues decreased significantly at all cadmium concentration levels except for 2 mg Kg-1. In addition, the interaction of cow manure and high concentrations of cadmium caused a decrease in nodule number, nodule fresh weights and total N content of shoot in common bean.
Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soilijtsrd
In-vivo experiment was conducted for toxicity evaluation of Imidacloprid on soil enzyme activities arylsulphatase, acid-phosphatase and dehydrogenase activities in the treated soil under control condition for 60 days at different application rate 3.97µg mL FR , 7.94µg mL 2FR and 39.7µg mL 10FR . Imidacloprid has significant toxic at 2FR and 10FR doses but not on the recommended field rate FR . Acid phosphatase activity was stimulated at FR rate, whereas at higher dose 10FR inhibited the activity. A significant drop of dehydrogenase activity was observed irrespective of doses at 30 days of application and after that the enzymatic activity slowly increased. Application of Imidacloprid at FR to agriculture soil of Tripura is not likely to show any detrimental effects on soil acid phosphatase and dehydrogenase activities but at higher elevated doses there appear some harmful effects which was also very much transient. Aloke Purkait | Dipak Kumar Hazra | Pabitra Kumar Biswas | Ashim Chowdhury "Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21406.pdf
Paper URL: https://www.ijtsrd.com/chemistry/analytical-chemistry/21406/studies-the-effects-of-imidacloprid-on-enzymatic-activities-in-clay-loam-soil/aloke-purkait
Monitoring of Selected Heavy Metals Uptake by Plant around Fagbohun Dumpsite,...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online
Bioremediating Effect of Glomus Hoi and Pseudomonas Aeruginosa on the Organic...IJEAB
This study analyzed the degrading effect of Glomus hoi and Pseudomonas aeruginosa on the organic content and heavy metals of oil refinery effluent polluted soil using Amaranthus cruentus as the test plant. This study was carried out to determine if agricultural activities can be improved using any or both of the micoorganisms. Eight different treatment layouts were used with three replicates for each level of pollution in the treatment layout. Ninety six (96) pots, each containing three kilograms of soil from both sterilized and unsterilized soil were used for the study. Fifty (50) grams of soil inoculum from propagated Arbuscular mycorrhiza was inoculated to a set of twenty four (24) experimental pots containing both sterilized and unsterilized soil before A. cruentus seedlings were transplanted to them. Another set of twenty four (24) pots containing both sterilized and unsterilized soil were injected with thirty (30) mL of P. aeruginosa inoculum solution before transplanting A. cruentus seedlings to them. The third set of twenty four (24) pots received dual inoculation of both fifty (50) grams of soil inoculum containing G. hoi and thirty (30) mL of P. aeruginosa inoculum solution before A. cruentus were transplanted to them. The residual twenty four (24) pots served as the control. Thereafter, pot preparation was arranged in the screenhouse in a randomized block design. The A. cruentus seedlings were raised in nursery for a period of two weeks before they were transplanted to the pots, seedlings were left for 3 days to overcome transplanting shock before contaminating the soil with refinery effluent at various concentrations of 0%, 2%, 4% and 6% v/w. The seedlings were allowed to grow for eight weeks before the termination of the experiment. The pre planting analysis of soil showed that heavy metals analyses (zinc and iron) of sterilized soil had a lower concentration to the unsterilized. The soil pH ranged from 6.3 to 6.8. It also revealed that organic matter and organic carbon content ranged from 0.8% to 1.3% and 0.4% to 1.7%. However, after the experiment, it was discovered in this study that treatments without any microorganism inoculation in sterilized and unsterilized soil had a higher level of % organic carbon and % organic matter content compared to the other treatments that were inoculated with one or two micro-organisms across all the levels of effluent concentration. Heavy metals of soil in all the soil samples were found to increase as the petrochemical effluent increased in concentration. The results obtained were analyzed using Duncan Multiple Range Test (DMRT) and other descriptive statistics. This study opined that the combined use of G. hoi and P. aeruginosa was more effective in improving the organic contentand the reduce heavy metals of oil refinery effluent polluted soil than when either is used singly.
Soil is a mixture of broken rocks and mineral which contain living organisms, in which microorganisms can transfer either by erosion or wind dispersal to other living things when come in close contact which can be as a source of contact to pathogenic microbes and can ponderous to health hazard of the community. This study investigates the microbial analysis and also to check the influence of soil with fallen rotten cocoa against soil without rotten cocoa on soil microbes in Ikeji-Arakeji, Osun state, Nigeria. Total bacterial and fungal counts were determined using pour plating method. Total bacterial count was higher from soil with fallen rotten cocoa pod 92 ×105 cfu/ml to soil without fallen rotten cocoa which is 86 ×105 cfu/ml and Total fungal count ranges was higher from soil with fallen rotten cocoa pod is 44 ×105 cfu/ml to soil without rotten cocoa pod which is 33 ×105 cfu/ml. The isolation and enumeration of microbial population was carried out using standard culture-based methods. Bacteria isolates such as Proteus vulgaris, Escherichia coli, Pseudomonas sp, Enterobacter aerogene., Bacillus spp, Staphylococcus spp, Micrococcus sp, Erysipelothrix spp and the fungi isolate include Aspergillus niger, Aspergillus flavus and Rhizopus stolonifer. All the microbes were present for the two samples except Micrococcus sp and Enterobacter aerogenes. The bacteria count were more alpine and higher in bacterial count than the fungal count. The presence of these microorganisms indicates a suitable soil for general plantation by the microbial degradative activities.
The effects of cadmium and cow manure on nodulation and growth attributes of ...Agriculture Journal IJOEAR
Abstract— Different biotic and abiotic factors are involved in the availability of heavy metals in soil including organic matter. In order to study the effects of cow manure on cadmium availability and their interactions on common bean nodulation and growth parameters, an experiment was conducted under greenhouse conditions. The treatments included five cadmium levels (0, 2, 5, 10, 20 mg cadmium per kg soil) and four cow manure levels (0, 15, 30, 60 t ha-1) based on a completely randomized design with three replications. Results indicated that increased soil cadmium concentrations caused higher cadmium uptake by root tissues whereas, Root nodulation and total N content of shoot tissues decreased significantly at all cadmium concentration levels except for 2 mg Kg-1. In addition, the interaction of cow manure and high concentrations of cadmium caused a decrease in nodule number, nodule fresh weights and total N content of shoot in common bean.
Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soilijtsrd
In-vivo experiment was conducted for toxicity evaluation of Imidacloprid on soil enzyme activities arylsulphatase, acid-phosphatase and dehydrogenase activities in the treated soil under control condition for 60 days at different application rate 3.97µg mL FR , 7.94µg mL 2FR and 39.7µg mL 10FR . Imidacloprid has significant toxic at 2FR and 10FR doses but not on the recommended field rate FR . Acid phosphatase activity was stimulated at FR rate, whereas at higher dose 10FR inhibited the activity. A significant drop of dehydrogenase activity was observed irrespective of doses at 30 days of application and after that the enzymatic activity slowly increased. Application of Imidacloprid at FR to agriculture soil of Tripura is not likely to show any detrimental effects on soil acid phosphatase and dehydrogenase activities but at higher elevated doses there appear some harmful effects which was also very much transient. Aloke Purkait | Dipak Kumar Hazra | Pabitra Kumar Biswas | Ashim Chowdhury "Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21406.pdf
Paper URL: https://www.ijtsrd.com/chemistry/analytical-chemistry/21406/studies-the-effects-of-imidacloprid-on-enzymatic-activities-in-clay-loam-soil/aloke-purkait
Monitoring of Selected Heavy Metals Uptake by Plant around Fagbohun Dumpsite,...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online
Bioremediating Effect of Glomus Hoi and Pseudomonas Aeruginosa on the Organic...IJEAB
This study analyzed the degrading effect of Glomus hoi and Pseudomonas aeruginosa on the organic content and heavy metals of oil refinery effluent polluted soil using Amaranthus cruentus as the test plant. This study was carried out to determine if agricultural activities can be improved using any or both of the micoorganisms. Eight different treatment layouts were used with three replicates for each level of pollution in the treatment layout. Ninety six (96) pots, each containing three kilograms of soil from both sterilized and unsterilized soil were used for the study. Fifty (50) grams of soil inoculum from propagated Arbuscular mycorrhiza was inoculated to a set of twenty four (24) experimental pots containing both sterilized and unsterilized soil before A. cruentus seedlings were transplanted to them. Another set of twenty four (24) pots containing both sterilized and unsterilized soil were injected with thirty (30) mL of P. aeruginosa inoculum solution before transplanting A. cruentus seedlings to them. The third set of twenty four (24) pots received dual inoculation of both fifty (50) grams of soil inoculum containing G. hoi and thirty (30) mL of P. aeruginosa inoculum solution before A. cruentus were transplanted to them. The residual twenty four (24) pots served as the control. Thereafter, pot preparation was arranged in the screenhouse in a randomized block design. The A. cruentus seedlings were raised in nursery for a period of two weeks before they were transplanted to the pots, seedlings were left for 3 days to overcome transplanting shock before contaminating the soil with refinery effluent at various concentrations of 0%, 2%, 4% and 6% v/w. The seedlings were allowed to grow for eight weeks before the termination of the experiment. The pre planting analysis of soil showed that heavy metals analyses (zinc and iron) of sterilized soil had a lower concentration to the unsterilized. The soil pH ranged from 6.3 to 6.8. It also revealed that organic matter and organic carbon content ranged from 0.8% to 1.3% and 0.4% to 1.7%. However, after the experiment, it was discovered in this study that treatments without any microorganism inoculation in sterilized and unsterilized soil had a higher level of % organic carbon and % organic matter content compared to the other treatments that were inoculated with one or two micro-organisms across all the levels of effluent concentration. Heavy metals of soil in all the soil samples were found to increase as the petrochemical effluent increased in concentration. The results obtained were analyzed using Duncan Multiple Range Test (DMRT) and other descriptive statistics. This study opined that the combined use of G. hoi and P. aeruginosa was more effective in improving the organic contentand the reduce heavy metals of oil refinery effluent polluted soil than when either is used singly.
Soil is a mixture of broken rocks and mineral which contain living organisms, in which microorganisms can transfer either by erosion or wind dispersal to other living things when come in close contact which can be as a source of contact to pathogenic microbes and can ponderous to health hazard of the community. This study investigates the microbial analysis and also to check the influence of soil with fallen rotten cocoa against soil without rotten cocoa on soil microbes in Ikeji-Arakeji, Osun state, Nigeria. Total bacterial and fungal counts were determined using pour plating method. Total bacterial count was higher from soil with fallen rotten cocoa pod 92 ×105 cfu/ml to soil without fallen rotten cocoa which is 86 ×105 cfu/ml and Total fungal count ranges was higher from soil with fallen rotten cocoa pod is 44 ×105 cfu/ml to soil without rotten cocoa pod which is 33 ×105 cfu/ml. The isolation and enumeration of microbial population was carried out using standard culture-based methods. Bacteria isolates such as Proteus vulgaris, Escherichia coli, Pseudomonas sp, Enterobacter aerogene., Bacillus spp, Staphylococcus spp, Micrococcus sp, Erysipelothrix spp and the fungi isolate include Aspergillus niger, Aspergillus flavus and Rhizopus stolonifer. All the microbes were present for the two samples except Micrococcus sp and Enterobacter aerogenes. The bacteria count were more alpine and higher in bacterial count than the fungal count. The presence of these microorganisms indicates a suitable soil for general plantation by the microbial degradative activities.
Impact of Compost Prepared from Invasive Alien Species in Alleviating Water S...YogeshIJTSRD
Invasive alien plant species are major thread to biodiversity, climate change and environmental sustainability. Management of these invasive alien plant species become a typical task at global level. Composting can be an efficient and environment friendly solution for management of these invasive alien species. The aim of present study was to evaluate the effect of compost prepared from three invasive alien species Cuscutareflexa, Eupatorium adenophorum and Lantana camaraon the tomato plant vigour, antioxidant and nutrient content under water deficit and irrigated well watered conditions. The results revealed that Cuscutareflexa CR compost treatment gave highest shoot length 23.0 , 23.7 , root length 30.0 , 21.4 , shoot fresh weight 47.9 , 52.2 , shoot dry weight 71.0 , 49.4 and root dry weight 66.7 , 51.5 , under water stressand irrigated conditions, respectively. The application of compostCR under water stress has enhanced chlorophyll and prolinecontent over control. Similarly, antioxidant enzymes analysis showed the increased superoxide dismutase 1.33 2.17fold , peroxidase 1.38 1.82fold and catalase 1.06 1.73fold activity under water deficit condition. Nutrient content such as nitrogen, phosphorus, potassium and sodiumin tomato leaf were higher under both water stress and irrigated conditions compared to their respective control. It can be concluded from above outcomes that compost prepared from invasive alien species have potential to ameliorate the negative effects of water stress and enhance the tomato growth. Sandhya Bind | A. K. Sharma "Impact of Compost Prepared from Invasive Alien Species in Alleviating Water Stress in Tomato (Solanum Lycopersicum L.)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-3 , April 2021, URL: https://www.ijtsrd.com/papers/ijtsrd39961.pdf Paper URL: https://www.ijtsrd.com/biological-science/botany/39961/impact-of-compost-prepared-from-invasive-alien-species-in-alleviating-water-stress-in-tomato-solanum-lycopersicum-l/sandhya-bind
Effectiveness of various salinity on leaf growth of Gazania | IJAARInnspub Net
Salt stress is induced by Nacl and it minimizes the growth owing to variation in interior plant structure. The present work was designed in line to investigate the behavior of Gazania harlequin (L.) seedlings were raised with dissimilar salt combinations (25 ppm, 50 ppm, 75 ppm & 100 ppm of NaCl solution). The CRD experiment with 4 repetitions was done. The NaCl treatments were repeated in 4 times with 20 days interval totally for 80 days after transplanting in pots. Anatomical observations were recorded by the microscope of in NaCl affected growth leaf. The results illustrated that salinity levels had a negative effect on anatomical characters as (xylem region, phloem region, cortex region, epidermis region and density of leaf lamina). So, it is concluded from the study that control treatment (without salinity effect) showed improved outcome comparatively others treatments which were under the saline situation. As the salinity increases ultimately it affects leaf anatomy of the plant.
Cost effective Analysis of Different Soil Samples to Check their Potential fo...YogeshIJTSRD
The present investigation was undertaken to isolate and screen the cellulase producing bacteria from soil. Cellulose is considered to be the most abundant carbohydrate on earth along with hemicelluloses and lignin, which constitutes the plant cell wall. Cellulolytic enzymes are the backbone of various industries including food, animal feed, brewing, wine, agricultural biomass refining, pulp, paper, textile and ethanol production. In this paper, cellulose degrading bacteria was isolated and screened from different soil samples from agriculture farm, waste dumping area and termite soil using Carboxymethyl cellulose CMC , wheat bran and waste paper plus nutrient agar as a selective medium. After screening, only five isolates KHU2, KHU8, KHU9, KHU13 and KHU14 were selected due to the maximum diameter of zone of substrate hydrolysis. The diameter of zone of substrate hydrolysis in NAM plates containing wheat bran for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 8±3mm, 21±2mm, 13±1mm, 12±1mm and 10±2mm, respectively, whereas the diameter of zone of substrate hydrolysis in NAM plates containing waste paper for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 13±1mm, 24±1mm, 17±1mm, 14±2mm and 15±1mm respectively. This was compared with the diameter of zone of substrate hydrolysis in NAM plates containing CMC cellulose was also measured for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 16±1mm, 25±1mm, 22±1mm, 19±2mm and 20±2mm. It was concluded that the diameter of zone of substrate hydrolysis on waste paper was nearly same as obtained with CMC cellulose and the highest diameter of zone of substrate hydrolysis was seen in isolate KHU8 25±1mm in NAM plates plus CMC cellulose from termite soil sample. So, termite soil has the maximum potential to produce cellulose degrading bacteria. Khushboo Pal | Anshika Sharma | Tanu Sharma | Dr. Divya Sharma "Cost-effective Analysis of Different Soil Samples to Check their Potential for Producing Cellulose Degrading Bacteria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43937.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/43937/costeffective-analysis-of-different-soil-samples-to-check-their-potential-for-producing-cellulose-degrading-bacteria/khushboo-pal
Chemical and Microbial Reactions Occurring during VermicompostingYogeshIJTSRD
Vermicomposting is a unique process that occurs in earthworms gut to convert organic wastes into organic fertilizer or vermicompost by using joint action of earthworms and microorganisms. Organic wastes used were the mixture of spent mushrooms waste, coconut husks, cow dung, vegetables residue, and leaf litter. Locally available earthworms are also used for vermicomposting but their mode of feeding is very slow and the earthworm which lives below the soil is also not suitable for vermicompost production. The Red worms Eisenia foetida and African earthworm Eudrillus engenae are promising worms used for vermicompost production. All the two worms can be mixed together for vermicompost production. In the composting process, egg shells flour and fish meal are added into the vermicompost as additives materials. The results indicate that the combined vermicomposting and composting process with addition the additives materials improves the chemical composition of vermicompost compared to using vermicomposting process alone. The percentage of nitrogen, phosphorous, potassium, calcium and magnesium in vermicompost was found to increase while C N ratio, pH and total organic carbon declined as a function of the vermicomposting. Dr. Atul Kumar Sharma | Dr. Kamlesh Rawat "Chemical and Microbial Reactions Occurring during Vermicomposting" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45029.pdf Paper URL: https://www.ijtsrd.com/chemistry/other/45029/chemical-and-microbial-reactions-occurring-during-vermicomposting/dr-atul-kumar-sharma
Rice is one of the most important cereal crops of developing countries and the staple food of about 65% of the world’s population. The rice crops have been greatly disturbed by the heavy metals. The present study deals with the toxic effect of sodium arsenate on morphological and molecular variation through SDS-PAGE in 10 rice (Oryza sativa L.) varieties. Ten varieties of rice were grown under different concentration (25 ppm, 50 ppm and 100 ppm) of sodium arsenate against control. Morphological parameters like shoot length, root length, leaf area and biomass showed marked differences among ten rice varieties. The proteins were separated through SDS-PAGE gel electrophoresis and calculated their molecular weight. The morphological and molecular variations induced in rice varieties by arsenic stress provide a new insight leading to a better understanding of the heavy metal response in plants.
Article Citation:
John De Britto R, Mary Sujin R, Steena Roshan Sebastian and Dharmar K.
Toxic effect of arsenic on ten rice varieties.
Journal of Research in Agriculture (2011) 1(1): 011-016.
Full Text:
http://www.jagri.info/documents/AG0003.pdf
Rice is one of the most important cereal crops of developing countries and
the staple food of about 65% of the world’s population. The rice crops have been
greatly disturbed by the heavy metals. The present study deals with the toxic effect of
sodium arsenate on morphological and molecular variation through SDS
-
PAGE in 10
rice (
Oryza sativa
L.) varieties. Ten varieties of rice were grown under different
concentration (25 ppm, 50 ppm and 100 ppm) of sodium arsenate against control.
Morphological parameters like shoot length, root length, leaf area and biomass
showed marked differences among ten rice varieties. The proteins were separated
through SDS
-
PAGE gel electrophoresis and calculated their molecular weight. The
morphological and molecular variations induced in rice varieties by arsenic stress
provide a new insight leading to a better understanding of the heavy metal response
in plants.
A study on physiological, anatomical characterization of selected carrot plan...Innspub Net
Carrots were first used for medical purposes and gradually used as food. It is also a good source of magnesium and manganese. Cadmium is a nonessential element that adversely affects plant growth and development. It is considered as one of the significant pollutants due to its high toxicity and more solubility in water. Experiment was conducted in Old Botanical Garden of University of Agriculture Faisalabad to check the response of carrot genotypes grown under Cadmium Chloride stress (0µM, 10µM, 15µM and 20µM). Variety of carrot (gajar) used was Red Gold. Seeds were sown in 12 pots, which were filled with sand, in 2nd week of November. Germination observed after one week of sowing. The experiment was laid out in a completely randomized design with three replicates. Seeds of carrot (red gold) were purchased from Ayyub Agriculture Research Institute (AARI) Faisalabad and sown directly in the plastic pot. 8 seeds per pot were distributed. Hogland solution was also applied to replicates and then I applied Cadmium Chloride stress to the plants to start my experiment. Cadmium stress decreased the uptake and distribution of essential elements in plant. Studies had revealed that heavy metals cause adverse effects on plant growth, which further lead to decrease plant yield and inhibition of enzymatic activities.In the present study, plant growth characteristics, root length and shoot length decreased under Cd stress. In fact, salt stress decreased all attributes in carrot when the concentrations of Cadmium chloride increased higher and higher.
Effect of potassium fertilization and organic nutrient (Reef Amirich) in the ...Innspub Net
A field study was conducted at the college of Agriculture, Baghdad University- Jadiriyah to investigate the effect of adding potassium fertilizer and organic nutrient (Reef Amirich) on the population density of two sucking pests of cucumber, cotton whitefly, Bemisia tabaci and onion thrips, Thrips tabaci during the spring season/2016. Results indicated that potassium sulphate (50, 100 and 150 kg/ha) and organic nutrient (0.8 and 1.6ml/l) reduced both the population density of B. tabaci and T. tabaci nymphs depending on the fertilizer level of the user, the treatment 150 kg/ha for the potassium fertilizer and 1.6 ml/L for organic nutrient was the highest among others when minimized density of nymphs by 1.62 nymphs of B. tabaci/disk leaf and 0.38 nymph of T. tabaci / disk leaf. suggesting a possible increase in cucumber resistance to this pest as a result of the treatments, and the result showed the infestation with B. tabaci was higher in the second half of May, the average number of nymph were 5.35 nymph/ disk leaf, while the highest number of T. tabaci was observed in the first half of May, the average number reached to 2.73 nymph/ disk leaf. The result showed the interaction treatment K150A1.6 was significant superior of percentage of K and increased of content in leaves of Fe, Cu and Zn.
Bioremediation Through The Use of Indigenous Natural Resources vis-a-vis Its ...Agriculture Journal IJOEAR
Abstract— An assessment was undertaken to study the combating capacity of bacterial consortia isolated from different sources viz. oil spillage sludge and water spillage of petrol pump and rhizosphere of rice plant against the fluoride toxicity under field condition. Oryza sativa was selected as a test species. The recommended doses of chemical fertilizers (70:35:35) and different concentration of sodium fluoride (25 , 50 , 100 , 200 , 300 , 400 and 500 mg Kg-1 F) were used for first set of experiment and the second set were treated with vermicompost, compost, bacterial consortia and different concentration of sodium fluoride(25 ; 50 , 100 , 200 , 300 , 400 and 500 mg Kg-1 F). Among all of the species Penicillium, Aspergillus and Fusarium were resistant and survived under fluoride polluted condition. One unique thing was observed from these experiment that paddy which were grown under indigenous organic inputs treated plots gave maximum yield under T1 treatment (25 mg Kg-1 F) which was above the control set. Moreover, stress enduring metabolites viz. proline content of flag leaves were lowest recorded under indigenous organic inputs treated plots as compared to chemical fertilizer treated plots. Data were significantly different at 5% level using Duncan’s Multiple Range Test. From the Air Pollution Tolerance Index (APTI) value of paddy clearly depicted that the crop of those plots were treated with indigenous organic inputs were more resistant for enduring stress condition. In these experiment combination of vermicompost, compost and bacterial consortia were capable of reducing the amount of fluoride within plant parts especially in grains (< 0.3 mg Kg-1 as recommended by EPA, FAO and WHO), where fluoride was within permissible range as well as they reduced the fluoride content within the soil (2.57-16.44 mg Kg-1. as recommended by FAO, EPA, and WHO) as was noted by measuring the fluoride in the plant parts and soil after the experiment. Therefore, bacterial consortia could be an alternative for bioremediation of fluoride.
Litter Generated Ammonia Captured by Activated Carbon Derived from Broiler Li...LPE Learning Center
http://www.extension.org/67575 In 2011, the production rate of broilers was 8.6 billion with a value of $23.2 billion (USDA 2012). Both CERCLA and EPCRA have reporting requirements for ammonia (NH3) of 100 lb of NH3/d or 18.3 tons/yr, a level that may affect large animal production facilities (NRC 2003). Although USEPA (2009) has provided an exemption for animal waste producing farms under CERCLA for reporting hazardous air emissions, it is expected that this exemption will be revoked once valid methodologies are established for monitoring. Two of the 24 sites in the NAEMS monitoring study reported similar NH3 emissions of 3.6 – 5.3 tons of NH3 per house per year (Burns et al. 2009, Heber 2010). Emissions of this level indicate a need for developing technologies that can reduce the NH3 levels produced by broiler operations. This research is focused on the use of broiler litter as activated carbon (BAC) to reduce aerial NH3 generated by litter, an opportunity to not only reuse the manure, but also treat the emissions from or within broiler houses. The objective of this study was to evaluate the efficacy of BAC to remove NH3 volatilized from litter samples in a laboratory acid-trap system. Preliminary studies using NH3/air mixture indicated that the BAC capacity to adsorb NH3 was approximately double that of Vapure 612, a commercial carbon. In the litter emission study, the BAC and Vapure performance was comparable. Breakthrough for both carbons occurred within 14 hours of the test start. At the end of the 3 day test, the NH3 emission for BAC was 75% of the litter only control, whereas, the Vapure emission was 64% of the control. The results of the study demonstrate the potential for a cyclical waste utilization strategy in using broiler litter activated carbon to capture NH3 volatilized from litter.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
Isolation, characterization of aspergillus fumigatus and optimization of cult...eSAT Journals
Abstract The soil samples were collected from different depths of paddy and sugarcane fields. The samples were primarily screened for isolation of amylase producing fungi. Among the isolated fungi, amylase producing isolates were identified by growing on starch agar media. The isolate (15F) which form the maximum zone of clearance on starch agar media by iodine was identified and it was subcultured on potato dextrose agar (PDA). The isolate (15F) was morphologically characterized by performing cotton blue staining and scanning electron microscopic observations under required magnifications. Molecular characterization of isolate (15F) was performed by ITS/5.8S rRNA and β-tubulin gene sequence analysis and it was confirmed as Aspergillus fumigatus (MTCC Acc No 11399). Optimization of cultural conditions for maximum production of amylase was carried out by different cereal flours, incubation periods, temperatures, nitrogen sources and with different phosphate concentrations. Aspergillus fumigatus showed maximum amylase activity (230±0.7U/mg protein) when cultured in finger millet at 350C for 72hrs of incubation period. Keywords: Amylase, Aspergillus fumigatus, cereal flour, submerged fermentation
Impact of Compost Prepared from Invasive Alien Species in Alleviating Water S...YogeshIJTSRD
Invasive alien plant species are major thread to biodiversity, climate change and environmental sustainability. Management of these invasive alien plant species become a typical task at global level. Composting can be an efficient and environment friendly solution for management of these invasive alien species. The aim of present study was to evaluate the effect of compost prepared from three invasive alien species Cuscutareflexa, Eupatorium adenophorum and Lantana camaraon the tomato plant vigour, antioxidant and nutrient content under water deficit and irrigated well watered conditions. The results revealed that Cuscutareflexa CR compost treatment gave highest shoot length 23.0 , 23.7 , root length 30.0 , 21.4 , shoot fresh weight 47.9 , 52.2 , shoot dry weight 71.0 , 49.4 and root dry weight 66.7 , 51.5 , under water stressand irrigated conditions, respectively. The application of compostCR under water stress has enhanced chlorophyll and prolinecontent over control. Similarly, antioxidant enzymes analysis showed the increased superoxide dismutase 1.33 2.17fold , peroxidase 1.38 1.82fold and catalase 1.06 1.73fold activity under water deficit condition. Nutrient content such as nitrogen, phosphorus, potassium and sodiumin tomato leaf were higher under both water stress and irrigated conditions compared to their respective control. It can be concluded from above outcomes that compost prepared from invasive alien species have potential to ameliorate the negative effects of water stress and enhance the tomato growth. Sandhya Bind | A. K. Sharma "Impact of Compost Prepared from Invasive Alien Species in Alleviating Water Stress in Tomato (Solanum Lycopersicum L.)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-3 , April 2021, URL: https://www.ijtsrd.com/papers/ijtsrd39961.pdf Paper URL: https://www.ijtsrd.com/biological-science/botany/39961/impact-of-compost-prepared-from-invasive-alien-species-in-alleviating-water-stress-in-tomato-solanum-lycopersicum-l/sandhya-bind
Effectiveness of various salinity on leaf growth of Gazania | IJAARInnspub Net
Salt stress is induced by Nacl and it minimizes the growth owing to variation in interior plant structure. The present work was designed in line to investigate the behavior of Gazania harlequin (L.) seedlings were raised with dissimilar salt combinations (25 ppm, 50 ppm, 75 ppm & 100 ppm of NaCl solution). The CRD experiment with 4 repetitions was done. The NaCl treatments were repeated in 4 times with 20 days interval totally for 80 days after transplanting in pots. Anatomical observations were recorded by the microscope of in NaCl affected growth leaf. The results illustrated that salinity levels had a negative effect on anatomical characters as (xylem region, phloem region, cortex region, epidermis region and density of leaf lamina). So, it is concluded from the study that control treatment (without salinity effect) showed improved outcome comparatively others treatments which were under the saline situation. As the salinity increases ultimately it affects leaf anatomy of the plant.
Cost effective Analysis of Different Soil Samples to Check their Potential fo...YogeshIJTSRD
The present investigation was undertaken to isolate and screen the cellulase producing bacteria from soil. Cellulose is considered to be the most abundant carbohydrate on earth along with hemicelluloses and lignin, which constitutes the plant cell wall. Cellulolytic enzymes are the backbone of various industries including food, animal feed, brewing, wine, agricultural biomass refining, pulp, paper, textile and ethanol production. In this paper, cellulose degrading bacteria was isolated and screened from different soil samples from agriculture farm, waste dumping area and termite soil using Carboxymethyl cellulose CMC , wheat bran and waste paper plus nutrient agar as a selective medium. After screening, only five isolates KHU2, KHU8, KHU9, KHU13 and KHU14 were selected due to the maximum diameter of zone of substrate hydrolysis. The diameter of zone of substrate hydrolysis in NAM plates containing wheat bran for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 8±3mm, 21±2mm, 13±1mm, 12±1mm and 10±2mm, respectively, whereas the diameter of zone of substrate hydrolysis in NAM plates containing waste paper for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 13±1mm, 24±1mm, 17±1mm, 14±2mm and 15±1mm respectively. This was compared with the diameter of zone of substrate hydrolysis in NAM plates containing CMC cellulose was also measured for the isolates KHU2, KHU8, KHU9, KHU13 and KHU14 was 16±1mm, 25±1mm, 22±1mm, 19±2mm and 20±2mm. It was concluded that the diameter of zone of substrate hydrolysis on waste paper was nearly same as obtained with CMC cellulose and the highest diameter of zone of substrate hydrolysis was seen in isolate KHU8 25±1mm in NAM plates plus CMC cellulose from termite soil sample. So, termite soil has the maximum potential to produce cellulose degrading bacteria. Khushboo Pal | Anshika Sharma | Tanu Sharma | Dr. Divya Sharma "Cost-effective Analysis of Different Soil Samples to Check their Potential for Producing Cellulose Degrading Bacteria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43937.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/43937/costeffective-analysis-of-different-soil-samples-to-check-their-potential-for-producing-cellulose-degrading-bacteria/khushboo-pal
Chemical and Microbial Reactions Occurring during VermicompostingYogeshIJTSRD
Vermicomposting is a unique process that occurs in earthworms gut to convert organic wastes into organic fertilizer or vermicompost by using joint action of earthworms and microorganisms. Organic wastes used were the mixture of spent mushrooms waste, coconut husks, cow dung, vegetables residue, and leaf litter. Locally available earthworms are also used for vermicomposting but their mode of feeding is very slow and the earthworm which lives below the soil is also not suitable for vermicompost production. The Red worms Eisenia foetida and African earthworm Eudrillus engenae are promising worms used for vermicompost production. All the two worms can be mixed together for vermicompost production. In the composting process, egg shells flour and fish meal are added into the vermicompost as additives materials. The results indicate that the combined vermicomposting and composting process with addition the additives materials improves the chemical composition of vermicompost compared to using vermicomposting process alone. The percentage of nitrogen, phosphorous, potassium, calcium and magnesium in vermicompost was found to increase while C N ratio, pH and total organic carbon declined as a function of the vermicomposting. Dr. Atul Kumar Sharma | Dr. Kamlesh Rawat "Chemical and Microbial Reactions Occurring during Vermicomposting" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45029.pdf Paper URL: https://www.ijtsrd.com/chemistry/other/45029/chemical-and-microbial-reactions-occurring-during-vermicomposting/dr-atul-kumar-sharma
Rice is one of the most important cereal crops of developing countries and the staple food of about 65% of the world’s population. The rice crops have been greatly disturbed by the heavy metals. The present study deals with the toxic effect of sodium arsenate on morphological and molecular variation through SDS-PAGE in 10 rice (Oryza sativa L.) varieties. Ten varieties of rice were grown under different concentration (25 ppm, 50 ppm and 100 ppm) of sodium arsenate against control. Morphological parameters like shoot length, root length, leaf area and biomass showed marked differences among ten rice varieties. The proteins were separated through SDS-PAGE gel electrophoresis and calculated their molecular weight. The morphological and molecular variations induced in rice varieties by arsenic stress provide a new insight leading to a better understanding of the heavy metal response in plants.
Article Citation:
John De Britto R, Mary Sujin R, Steena Roshan Sebastian and Dharmar K.
Toxic effect of arsenic on ten rice varieties.
Journal of Research in Agriculture (2011) 1(1): 011-016.
Full Text:
http://www.jagri.info/documents/AG0003.pdf
Rice is one of the most important cereal crops of developing countries and
the staple food of about 65% of the world’s population. The rice crops have been
greatly disturbed by the heavy metals. The present study deals with the toxic effect of
sodium arsenate on morphological and molecular variation through SDS
-
PAGE in 10
rice (
Oryza sativa
L.) varieties. Ten varieties of rice were grown under different
concentration (25 ppm, 50 ppm and 100 ppm) of sodium arsenate against control.
Morphological parameters like shoot length, root length, leaf area and biomass
showed marked differences among ten rice varieties. The proteins were separated
through SDS
-
PAGE gel electrophoresis and calculated their molecular weight. The
morphological and molecular variations induced in rice varieties by arsenic stress
provide a new insight leading to a better understanding of the heavy metal response
in plants.
A study on physiological, anatomical characterization of selected carrot plan...Innspub Net
Carrots were first used for medical purposes and gradually used as food. It is also a good source of magnesium and manganese. Cadmium is a nonessential element that adversely affects plant growth and development. It is considered as one of the significant pollutants due to its high toxicity and more solubility in water. Experiment was conducted in Old Botanical Garden of University of Agriculture Faisalabad to check the response of carrot genotypes grown under Cadmium Chloride stress (0µM, 10µM, 15µM and 20µM). Variety of carrot (gajar) used was Red Gold. Seeds were sown in 12 pots, which were filled with sand, in 2nd week of November. Germination observed after one week of sowing. The experiment was laid out in a completely randomized design with three replicates. Seeds of carrot (red gold) were purchased from Ayyub Agriculture Research Institute (AARI) Faisalabad and sown directly in the plastic pot. 8 seeds per pot were distributed. Hogland solution was also applied to replicates and then I applied Cadmium Chloride stress to the plants to start my experiment. Cadmium stress decreased the uptake and distribution of essential elements in plant. Studies had revealed that heavy metals cause adverse effects on plant growth, which further lead to decrease plant yield and inhibition of enzymatic activities.In the present study, plant growth characteristics, root length and shoot length decreased under Cd stress. In fact, salt stress decreased all attributes in carrot when the concentrations of Cadmium chloride increased higher and higher.
Effect of potassium fertilization and organic nutrient (Reef Amirich) in the ...Innspub Net
A field study was conducted at the college of Agriculture, Baghdad University- Jadiriyah to investigate the effect of adding potassium fertilizer and organic nutrient (Reef Amirich) on the population density of two sucking pests of cucumber, cotton whitefly, Bemisia tabaci and onion thrips, Thrips tabaci during the spring season/2016. Results indicated that potassium sulphate (50, 100 and 150 kg/ha) and organic nutrient (0.8 and 1.6ml/l) reduced both the population density of B. tabaci and T. tabaci nymphs depending on the fertilizer level of the user, the treatment 150 kg/ha for the potassium fertilizer and 1.6 ml/L for organic nutrient was the highest among others when minimized density of nymphs by 1.62 nymphs of B. tabaci/disk leaf and 0.38 nymph of T. tabaci / disk leaf. suggesting a possible increase in cucumber resistance to this pest as a result of the treatments, and the result showed the infestation with B. tabaci was higher in the second half of May, the average number of nymph were 5.35 nymph/ disk leaf, while the highest number of T. tabaci was observed in the first half of May, the average number reached to 2.73 nymph/ disk leaf. The result showed the interaction treatment K150A1.6 was significant superior of percentage of K and increased of content in leaves of Fe, Cu and Zn.
Bioremediation Through The Use of Indigenous Natural Resources vis-a-vis Its ...Agriculture Journal IJOEAR
Abstract— An assessment was undertaken to study the combating capacity of bacterial consortia isolated from different sources viz. oil spillage sludge and water spillage of petrol pump and rhizosphere of rice plant against the fluoride toxicity under field condition. Oryza sativa was selected as a test species. The recommended doses of chemical fertilizers (70:35:35) and different concentration of sodium fluoride (25 , 50 , 100 , 200 , 300 , 400 and 500 mg Kg-1 F) were used for first set of experiment and the second set were treated with vermicompost, compost, bacterial consortia and different concentration of sodium fluoride(25 ; 50 , 100 , 200 , 300 , 400 and 500 mg Kg-1 F). Among all of the species Penicillium, Aspergillus and Fusarium were resistant and survived under fluoride polluted condition. One unique thing was observed from these experiment that paddy which were grown under indigenous organic inputs treated plots gave maximum yield under T1 treatment (25 mg Kg-1 F) which was above the control set. Moreover, stress enduring metabolites viz. proline content of flag leaves were lowest recorded under indigenous organic inputs treated plots as compared to chemical fertilizer treated plots. Data were significantly different at 5% level using Duncan’s Multiple Range Test. From the Air Pollution Tolerance Index (APTI) value of paddy clearly depicted that the crop of those plots were treated with indigenous organic inputs were more resistant for enduring stress condition. In these experiment combination of vermicompost, compost and bacterial consortia were capable of reducing the amount of fluoride within plant parts especially in grains (< 0.3 mg Kg-1 as recommended by EPA, FAO and WHO), where fluoride was within permissible range as well as they reduced the fluoride content within the soil (2.57-16.44 mg Kg-1. as recommended by FAO, EPA, and WHO) as was noted by measuring the fluoride in the plant parts and soil after the experiment. Therefore, bacterial consortia could be an alternative for bioremediation of fluoride.
Litter Generated Ammonia Captured by Activated Carbon Derived from Broiler Li...LPE Learning Center
http://www.extension.org/67575 In 2011, the production rate of broilers was 8.6 billion with a value of $23.2 billion (USDA 2012). Both CERCLA and EPCRA have reporting requirements for ammonia (NH3) of 100 lb of NH3/d or 18.3 tons/yr, a level that may affect large animal production facilities (NRC 2003). Although USEPA (2009) has provided an exemption for animal waste producing farms under CERCLA for reporting hazardous air emissions, it is expected that this exemption will be revoked once valid methodologies are established for monitoring. Two of the 24 sites in the NAEMS monitoring study reported similar NH3 emissions of 3.6 – 5.3 tons of NH3 per house per year (Burns et al. 2009, Heber 2010). Emissions of this level indicate a need for developing technologies that can reduce the NH3 levels produced by broiler operations. This research is focused on the use of broiler litter as activated carbon (BAC) to reduce aerial NH3 generated by litter, an opportunity to not only reuse the manure, but also treat the emissions from or within broiler houses. The objective of this study was to evaluate the efficacy of BAC to remove NH3 volatilized from litter samples in a laboratory acid-trap system. Preliminary studies using NH3/air mixture indicated that the BAC capacity to adsorb NH3 was approximately double that of Vapure 612, a commercial carbon. In the litter emission study, the BAC and Vapure performance was comparable. Breakthrough for both carbons occurred within 14 hours of the test start. At the end of the 3 day test, the NH3 emission for BAC was 75% of the litter only control, whereas, the Vapure emission was 64% of the control. The results of the study demonstrate the potential for a cyclical waste utilization strategy in using broiler litter activated carbon to capture NH3 volatilized from litter.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
Isolation, characterization of aspergillus fumigatus and optimization of cult...eSAT Journals
Abstract The soil samples were collected from different depths of paddy and sugarcane fields. The samples were primarily screened for isolation of amylase producing fungi. Among the isolated fungi, amylase producing isolates were identified by growing on starch agar media. The isolate (15F) which form the maximum zone of clearance on starch agar media by iodine was identified and it was subcultured on potato dextrose agar (PDA). The isolate (15F) was morphologically characterized by performing cotton blue staining and scanning electron microscopic observations under required magnifications. Molecular characterization of isolate (15F) was performed by ITS/5.8S rRNA and β-tubulin gene sequence analysis and it was confirmed as Aspergillus fumigatus (MTCC Acc No 11399). Optimization of cultural conditions for maximum production of amylase was carried out by different cereal flours, incubation periods, temperatures, nitrogen sources and with different phosphate concentrations. Aspergillus fumigatus showed maximum amylase activity (230±0.7U/mg protein) when cultured in finger millet at 350C for 72hrs of incubation period. Keywords: Amylase, Aspergillus fumigatus, cereal flour, submerged fermentation
This slide is only for educational purpose. It is based on the diseases prevention program for commercial broiler production. I believed that by using this ppt students would be benefited who are particularly in this discipline.
Microbiological and physicochemical analyses of top soils obtained from four ...Innspub Net
Several methodologies were utilized to evaluate the microbiological and physico chemical properties of top soil samples bored from four municipal waste dumpsites and a farmland (control sample) all located in Benin City, Edo State. The soil samples were obtained during the month of January, 2013. The mean aerobic bacterial counts for the soil samples ranged from 9.7 × 103 cfu/g for the control soil to 1.80 × 104 cfu/g for the soil sourced from the dump site at Ikheuniro. The mean heterotrophic fungal counts varied from 7.0 × 102 cfu/g for capitol dumpsite to 3.3 × 103 cfu/g for the control soil. Ten (10) microbial isolates were characterized and identified; Bacillus sp., Pseudomonas sp., Aeromonas sp., Enterobacter sp., Klebsiella sp. and Staphylococcus sp., Aspergillus sp., Mucor sp., Saccharomyces sp. and Fusarium sp. respectively. Both Bacillus sp. and Pseudomonas sp. were the most dominant amongst the bacterial isolates whilst Staphylococcus sp. was the least occurring bacterial isolate. Aspergillus sp. was the highest occurring fungal isolate while the least isolated fungal culture was Saccharomyces sp. The physico chemical results showed values which ranged from 5.60 to 8.08, 164.00 µS/cm to 540.00 µS/cm, 2.378 mg/kg to 3.444 mg/kg, 0.009 mg/kg to 0.016 mg/kg for pH, electrical conductivity, sulphate and cadmium. Despite the positive impacts of the dumped municipal wastes on the microbial and organic properties of the analyzed soils, disposal of municipal wastes in open dump sites is an archaic and unsustainable option in the management of municipal wastes. Get the full articles at: http://www.innspub.net/volume-1-number-1-september-2013/
Biochar impact on physiological and biochemical attributes of spinachGJESM Publication
Disastrous effect of nickel on spinach was discussed by number of authors but the effect of amendments like biochar with nickel on Spinacea oleraceaL. is not still discussed by any author of the world because biochar was used as soil amendments which play a vital role in reducing mobilization and uptake of nickel by spinach plants. As nickel contaminated plants are very harmful for the consumption by living organisms. Nickel can be gathered in agronomic soils by anthropogenic actions such as Ni-Cd batteries. In this study, the growth, physiological, photosynthetic and biochemical responses of Spinacia oleracea grown in Ni-spiked soil (0, 25, 50 and 100 mg Ni/Kg soil) at three levels of cotton-sticks-derived biochar “CSB” (0, 3 and 5 %) were evaluated. The results exposed significant decrease in growth, photosynthetic, physiological, and biochemical traits of S. oleracea when grown in Ni-polluted soil. However, this decrease was less pronounced in CSB amended soil. A steady rise in the MDA (0.66 µg/g to 2.08 µg g-1), ascorbic acid (1.24 mg/g to 1.57 mg/g)and sugar concentrations (1.73 mg/g to 2.16 mg/g)was observed with increased concentration of Ni. The increasing percentages of CSB from 3 % to 5 % decreased Ni concentrations in root and shoot of experimental plant. Higher production of chlorophyll, amino acids and protein with CSB amendment looked like alleviation in Ni toxicity. Therefore, it is concluded that, Ni toxicity and availability to the plants can be reduced by CSB amendments.
The present study intended to compare the efficiency of the wetland plants Phragmites australis (cav.)
Trin. ex Steudel and Cyperus papyrus L. for the accumulation of heavy metals. Maximum accumulation
of the heavy metals Pb (132.5-175 μg/g DW), Zn (97.5-100 μg/g DW) and Cr (80.5-90 μg/g DW) was
recorded in roots of the two wetland plants C. papyrus and P. australis, respectively. Cadmium showed
the lowest metal accumulation in the tissues of both plants (0.55-5.5 μg/g DW). C. papyrus roots
accumulated higher levels of most metals than those of P. australis. Heavy metals were shown to be not
only accumulated in roots of both plants but also were translocated to the shoots and accumulated in the
harvestable plant parts. The results showed variation in the levels of the heavy metals accumulated in the
different parts of C. papyrus and P. australis, in spite of being planted at the same site and subjected to
the same conditions. The potential use of Phragmites australis and Cyperus papyrus in phytoremediation
is also discussed.
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Evaluation of Heavy Metals in Commonly Edible Vegetables available in Markets...AI Publications
Vegetables are considered vital given that they supply to humans vitamins, minerals, nutritional fiber, and phytochemicals. This study aimed to evaluate the concentrations of heavy metals in common vegetables, locally available in Akure South LGAs markets for consumers. Samples of vegetables belonging to Amaranthaceae, Malvaceae, Cucurbitaceae, and Basellaceae families were procured from local markets of different areas of Akure south LGAs and analyzed for Cu, Pb, Fe, Zn and Mnby Atomic Absorption Spectrophotometry (AAS). The results indicated that the trace metal content of Telfariaoccidentalis, Corchorusolitorus, Amaranthushybridusand Abelmoschusesculentus from Oja Oba were higher than the other markets. Basellaalba purchased from Isikan possessed the highest metal content. Maximum concentration of Fe was found to be 1.93μg/g in Basellaalba, Zn 0.76 μg/g in Basella alba, Mn 1.12 μg/g in Basella alba, Cu 0.76μg/g in Telfairiaoccidentalis. The overall content of metals appeared to be within the limit laid down for safe human consumption.
Proximate and heavy metals composition of Plantain (Musa paradisiaca L.) frui...IJEAB
Plantain thrives well in waste dumpsites. These wastes usually contaminate the soil with heavy metals which become absorbed by the plants. The risk of heavy metal toxicity in humans is an issue of serious concern globally. Proximate composition of plantain fruits harvested from three randomly selected waste dumpsites in Uyo metropolis, Nigeria were determined using AOAC standard analytical techniques while their heavy metals (Pb, Cr, Ni, Cu, Co, Cd and As) concentrations and that of their rhizosphere soil were analysed with Unicam Atomic Absorption Spectrophotometer. Proximate analyses results revealed that carbohydrate content was higher in the fruits harvested from the control uncontaminated soil sites (91.61%) than in the fruits harvested from dumpsite soils (87.23%; 87.89%; 88.00%). Dumpsite soils had higher heavy metals concentrations than the control soil. Lead (Pb) was the highest occurring heavy metal in all the dumpsite soils. Fruits harvested from the dumpsite soils had higher heavy metals concentrations than those from the control soil. Pb was the only heavy metal whose concentration in the plantain fruits was higher than the WHO/FAO permissible limit. This work has established that the selected dumpsite soils have been contaminated with heavy metals which have been absorbed by the plantain cultivated there. Cultivation and consumption of plantain from these dumpsite soils should be discouraged.
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Uptake and translocation of copper by mycorrhized seedlings Sterculia setiger...researchagriculture
Pot culture experiments were established to determine the effects of arbuscular mycorrhizal fungus (AMF) (Glomus fasciculatum) on tropical gum tree (Sterculia setigera Del.) grown in Copper contaminated soils. AMF and non-AMF inoculated plants were grown in sterilized substrates and subjected to different copper level (0, 200, 400,600, 800 mg kg-1) concentrations. Root and shoot biomasses of inoculated plants were significantly higher than those of non-inoculated. Copper concentrations in roots were significantly higher than those in shoots in both the inoculated and non-inoculated plants, indicating this heavy metal mostly accumulated in the roots of plants. Copper translocation efficiency from root to shoot was lower in mycorrhizal plants than in nonmycorrhizal ones at any Copper addition levels. However, at high soil Copper concentrations, shoot Copper concentration of inoculated plant were significantly reduced by about 50% compared to non-inoculated plants. These results indicated that AMF could promote tropical gum tree growth and decrease the uptake of Cu at higher soil concentrations, thus protecting their hosts from the toxicity of Copper contaminated soils.
Article Citation:
Malick Ndiaye, Cavalli Eric, Diouf Adama, Diop Tahir Abdoulaye.
Uptake and translocation of copper by mycorrhized seedlings Sterculia setigera (Del.) under Copper-contamined soil.
Journal of Research in Agriculture (2012) 1(1): 022-028.
Full Text:
http://www.jagri.info/documents/AG0012.pdf
Uptake and translocation of copper by mycorrhized seedlings Sterculia setige...researchagriculture
Pot culture experiments were established to determine the effects of
arbuscular mycorrhizal fungus (AMF) (
Glomus fasciculatum
) on tropical gum tree
(
Sterculia setigera
Del.) grown in Copper contaminated soils. AMF and non
-
AMF
inoculated plants were grown in sterilized substrates and subjected to different
copper level (0, 200, 400,600, 800 mg kg
-
1
) concentrations. Root and shoot biomasses
of inoculated plants were significantly higher than those of non
-
inoculated. Copper
concentrations in roots were significantly higher than those in shoots in both the
inoculated and non
-
inoculated plants, indicating this heavy metal mostly accumulated
in the roots of plants. Copper translocation efficiency from root to shoot was lower in
mycorrhizal plants than in nonmycorrhizal ones at any Copper addition levels.
However, at high soil Copper concentrations, shoot Copper concentration of
inoculated plant were significantly reduced by about 50% compared to non
-
inoculated plants. These results indicated that AMF could promote tropical gum tree
growth and decrease the uptake of Cu at higher soil concentrations, thus protecting
their hosts from the toxicity of Copper contaminated soils.
International Journal of Computational Engineering Research (IJCER) is dedicated to protecting personal information and will make every reasonable effort to handle collected information appropriately. All information collected, as well as related requests, will be handled as carefully and efficiently as possible in accordance with IJCER standards for integrity and objectivity.
Germination and seedling growth of Moringa oleifera, Moringa stenopetala and ...Open Access Research Paper
A germination test was carried out to identify plants that can germinate and survive in polluted soil (with and without ash) collected 2.5km east and 2.5km west, 20km west and 55km west (control) of the BCL Cu/Ni mine smelter in Selebi-Phikwe, Botswana. The experiment was carried out using Phaseolus vulgaris, Moringa oleifera and Moringa stenopetala. Soil acidity and heavy metal stress reduced germination percentage, coefficient rate of germination, root and shoot growth and dry weight, root: shoot, vigour index and tolerance index of all species. Percentage reduction followed the order 2.5km west < 20km west < 2.5km east < 55km west. Phaseolus vulgaris, Moringa oleifera and Moringa stenopetala germinated in all soils. Their ability to germinate in polluted soil indicates tolerance to heavy metal and soil acidity stress and so they have potential for use in phytoremediation of polluted soils around the mine. Phaseolus vulgaris had the highest overall germination performance but there was no significant difference between the Moringas. Application of coal fly ash increased all the germination parameters and so coal fly ash has potential for use in amending polluted soil around the mine for phytoremediation purposes.
Germination and seedling growth of Moringa oleifera, Moringa stenopetala and ...Innspub Net
A germination test was carried out to identify plants that can germinate and survive in polluted soil (with and without ash) collected 2.5km east and 2.5km west, 20km west and 55km west (control) of the BCL Cu/Ni mine smelter in Selebi-Phikwe, Botswana. The experiment was carried out using Phaseolus vulgaris, Moringa oleifera and Moringa stenopetala. Soil acidity and heavy metal stress reduced germination percentage, coefficient rate of germination, root and shoot growth and dry weight, root: shoot, vigour index and tolerance index of all species. Percentage reduction followed the order 2.5km west < 20km west < 2.5km east < 55km west. Phaseolus vulgaris, Moringa oleifera and Moringa stenopetala germinated in all soils. Their ability to germinate in polluted soil indicates tolerance to heavy metal and soil acidity stress and so they have potential for use in phytoremediation of polluted soils around the mine. Phaseolus vulgaris had the highest overall germination performance but there was no significant difference between the Moringas. Application of coal fly ash increased all the germination parameters and so coal fly ash has potential for use in amending polluted soil around the mine for phytoremediation purposes.
The Influence of Soil Organic Matter on the Uptake of Silver Nanoparticles in...Agriculture Journal IJOEAR
— The uptake of silver from silver nanoparticles in soil was investigated in the presence of increasing concentrations of soil organic matter. Especially, the effect of Humus component of soil organic matter on the uptake of Ag from silver nanoparticles was studied. Two insect species, Acheta domesticus and Tenebrio molitor, and two plant species, Helianthus annuus and Sorghum vulgare, were exposed to silver nanoparticles(25 ppm in the presence of increasing concentrations of Humus (0, 1, 5, 10, 15, and 20% Humus) in soil (by weight). The techniques of transmission electron microscopy, dynamic light scattering, and powder X-ray diffraction were used to characterize the silver nanoparticles used in the study. An inductively coupled plasma-optical emission spectrometer was used to measure the levels of silver in test samples. Increasing concentrations of Humus in soil has resulted in an increase in the sulfur content and cation exchange capacity of the soil. A general decrease in the concentrations of silver was observed in Acheta domesticus and both the plant species, as a function of increasing concentrations of Humus in soil. In the case of plant species, the accumulation of silver nanoparticles was predominantly observed in the root tissue. Additionally, the translocation of silver from the roots to other plant tissues was observed in the case of Helianthus annuus. Results from this study suggest that the presence of Humus in soil could possibly decrease the uptake of silver from silver nanoparticles by insect and plant species.
1. 1
E3 Journal of Microbiology Research Vol. 1(1). pp. 001-008, June, 2013
Available online http://www.e3journals.org
Full length research paper
Tolerance potential of different Species of Aspergillus
as bioremediation tool - Comparative analysis
Shazia Iram1
, Kousar Perveen1
*, Naila Shuja1
, Kanwal Waqar1
, Ijaz Akhtar1
, Iftikhar Ahmad2
1
Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi.
2
National Agricultural Research Center, Islamabad, Pakistan
Accepted April 30, 2013
Due to increase of industrialization in public and private sectors along with urbanization, are reflected in
varying degree of pollution in the different compartments (water, soil and air) of environment. The present
study has been carried out to understand the tolerance potential of different species of Aspergillus (Aspergillus
niger, Aspergillus flavus, Aspegillus fumigatus) isolated from agriculture soil of Kasur irrigated with water
contaminated with sewage and industrial effluent. The degree of tolerance of fungi was measured by minimum
inhibitory concentration in the presence of different concentration of metals (Cr and Pb) and compared to
control sample. Results are shown the variation in the tolerance level of different isolates of Aspergillus. Few
isolates are tolerant, moderately tolerant and some are sensitive.
Keywords: Heavy metals; Tolerance potential; Aspergillus; MIC; Chromium; Lead
INTRODUCTION
Contamination of sediments and natural aquatic
receptors with different pollutant is a major environmental
problem all over the world (Baldrian and Gabriel, 2002;
Gavrilesca, 2004; Malik, 2004). Industrialization and
urbanization especially in developing countries have led
to the accumulation of heavy metals and petroleum
hydrocarbons in the environment (Adveniyi, 1996;
Bamgbose and Sibanjo, 1998; Byomi et al., 1999; Manay
et al., 1999; Ngodigha et al., 1999; Yamasoet et al., 2000;
Adedniyi and Folabi, 2002). Metals that are released into
the environment tend to persist indefinitely, accumulating
in to living tissues and posing a serious threat to the
environment and public health. These harmful
substances accumulate in crops via food chain; find their
way into our bodies, where they can cause a variety of
illness.
They are cytogenic, mutagenic, and carcinogenic in
nature and are posing threats to the urban population,
which rely on vegetables and foliage crops grown in pre-
urban lands. Introduction of heavy metal in environment
generally induces morphological and physiological
changes in microbial communities (Vadkertiova and
*Corresponding Author email kosar_ahmed111@yahoo.com
Slavikova, 2006), hence exerting a selective pressure on
the microbiota (Verma et al., 2001). These contaminated
sites are source of metal resistance microorganisms
(Gadd, 1993).
Among microorganisms fungi are very important
microorganism; it can tolerate heavy metals to a limit and
can also help to remove heavy metals from contaminated
soil. Fungi and yeast biomasses are known to tolerate
heavy metals (Khan, 2001, Baldrian, 2003; Gavrilesca,
2004). They are a versatile group, as they can adapt and
grow under various extreme conditions of pH,
temperature and nutrient availability, as well as high
metal concentrations (Anand et al., 2006).They offer the
advantage of having cell wall material which shows
excellent metal-binding properties (Gupta et al., 2000).
They are used for bioremediation because of their
mycelia nature and ability to accumulate metals of all
families.
Many fungal species such as Rhizopus arrhizus,
Penicilium spinulum and Aspergillus niger have been
extensively studied for heavy metals biosorption and the
process mechanism seems to be dependent upon
species (Zhou and Kiff, 1991; Hafez et al., 1997 ; Kapoor
and Viraraghavan, 1997). The accumulation of heavy
metals in agricultural soils is of increasing concern due to
food safety issues and potential health risks as well as its
2. 2
002 E3 J. Microbiol. Res
Figure 1: Map showing sampling sit
determinate effects on soil ecosystem. Farmers in big
cities where water from natural surface rain is not
available use sewerage water and water of natural drains
for crop production, due their being less expensive.
In most parts of Pakistan, untreated city effluent is
utilized for growing vegetables around large urban
settlements such as Kasur. Farmers use it as a source of
irrigation water and plant nutrients. However, its
continuous use may have serious environmental
implications, since it also contains heavy metals. Use of
untreated city effluent for irrigation without risk
assessment and management could be a serious hazard,
impacting soil and crop quality and ultimately human
health.
In Pakistan, heavy metals contaminated land is
becoming an environmental and economic issue.
Combination of inadequately plan effluent disposal
techniques and a rapidly growing population has lead to
gradual accumulation of heavy metals in the soils and
water of Pakistan. According to EPA, the industrial
effluents being dumped into the land, by electroplating,
tanner industries and textile mills and contains toxic
metals such as Cr, Ni, As, Hg, Cu, and Pb, which have
contaminated the soil and the biota residing in and
around soils in Pakistan (EPA, 1990). Over the years
there have been increasing concerns over the rising level
of heavy metal contamination, as they tend to persist in
the environment, accumulating in the biota, thereby
entering the food chain causing deleterious harm to
higher animals and humans (Zhang et al., 1998).
The aim of present study was to check the tolerance
potential of different species of Aspergillus was
investigated against heavy metals. For the present study
soil samples were collected from peri-urban agricultural
areas of Kasur which is irrigated by the contaminated
industrial water and containing heavy metals and toxic
chemicals. Fungi were isolated from the contaminated
soil and studied for their tolerance analysis.
Materials and Methods
Sampling and Sampling site: The main purpose of the
present study was to evaluate the tolerance potential of
different isolated fungal strain towards heavy metals (Pb,
Cr). For this purpose, soil samples were collected from
peri-urban agricultural areas of Kasur (Figure 1). The
agriculture land of Kasur is contaminated by sewage and
industrial effluents from the nearby industries and
contains heavy metals and toxic chemicals. From the
contaminated soil fungi were isolated and preserved for
further detail investigation of heavy metal tolerance.
Sterilization of Apparatus: Petri plates, media bottles,
distilled water, McCartney bottles and syringes were
sterilized in autoclave. For sterilization purpose all
apparatus were autoclaved for 40 minutes at 121°C. After
autoclaving all sterilized material dried in oven at 95°C.
Media Preparation and Fungal Isolation: Potato
Dextrose Agar (PDA) media was used for the isolation of
fungi. For the preparation of PDA, potatoes (200g) were
peeled, sliced and boiled, and then sieved through a
clean muslin cloth to get a broth in which agar (7.5g) and
dextrose sugar (7.5g) was added. The media was then
autoclaved for 30 minutes at 121°C (Martin, 1995). Fungi
were isolated on Potato Dextrose Agar (PDA) by soil
dilution method (Razak et al., 1999).
3. 3
Shazia et al. 003
Table 1: List of fungal isolates
Isolated fungus Sample code
Aspergillus fumigatus K3, K4, K12, K18, K26, K27, K28, K29, K30
Aspergillus niger K 14, K 16, K 22, K 23, K 24
Aspergillus flavus K 5, K 7, K 17, K 33, K 34, K 36, K 35, K 37, K 41
Preparation of plates
Fungi were isolated on Potato Dextrose Agar (PDA) by
soil dilution method (Razak et al., 1999).Poured the
media in Petri-dishes and allowed to solidify for 24 hour.
To suppress the bacterial growth, 30 mg/lit of
streptomycin was added in the medium (Martin, 1995).
Once the agar was solidified, and then put plates in an
inverted position for 24 hours at room temperature
(Martin, 1995).
Identification of fungi:
The fungal cultures were identified on the basis of
macroscopic (colonial morphology, color, texture, shape,
diameter and appearance of colony) and microscopic
(septation in mycelium, presence of specific reproductive
structures, shape and structure of conidia, and presence
of sterile mycelium) characteristics (Table 1) (Zafar et al.,
2006). Pure cultures of fungi isolates were identified with
the help of literature (Domsch et al., 1980; Barnett and
Hunter 1999).
Heavy metal tolerance test experiment
For the evaluation of tolerance potential among isolated
fungal strains, Potato Dextrose Agar (PDA) medium was
prepared and amended with various concentrations(0,
200, 400, 600, 800 and 1000 mg/l) of heavy
metal{Cr(NO3)3.9H2O and Pb(NO3)2} and 5.6 pH was
maintained by adding 5 molar solution of NaOH. Media
was autoclaved for 20 minutes at 121°C and poured in
Petri plates. The plates were incubated at 29ºC for 7 days.
The growth of fungi was monitored from the point of
inoculation or centre of the colony. Tolerance is
measured by observing minimum inhibitory concentration
(MIC) and tolerance index.
Heavy metals analysis of soil
Each soil sample (1g) was taken in the conical flask
(50ml), added 10ml of HNO3:HClO4 (1:2) solution (50ml)
and heated for half an hour. Solutions were filtered
through Whatman No. 1 filter paper and volume was
made to 50ml by adding distilled water. Soil samples
were digested in triplicates and analyzed for Zn, Cd, Cr,
Cu, Ni and Pb. The blank was prepared for quality
assurance of samples. The blank sample contained 10ml
of HNO3:HClO4 (1:2) solution and heated for half an hour
and volume was made 50ml by adding distilled water. For
the determination of heavy metals the atomic absorption
spectrophotometer was powered on and warmed up for
30 minutes. After the heating of cathode lamp, the air
acetylene flame was ignited and instrument was
calibrated or standardized with different working
standards (Vanloon and Lichwa, 1973)
Statistical analysis
The experiments were set up with three replicates.
Analysis of variance was performed by using statistical
software (SPSS 17) to compare resistance to metal
among individual isolates.
RESULTS AND DISCUSSION
Heavy metals released to the environment have been
increasing continuously as a result of the industrial
activities and technological development and poses
significant threat. Application of domestic and industrial
effluents to nearby agricultural fields is a common
practice of irrigation and wastewater disposal in Pakistan
(Hashem, 1995; Hafez et al., 1997). When wastewater is
applied to agricultural fields, heavy metals enter the soil
and get fixed into the soil components. Thus continuous
application of wastewater tends to accumulate large
quantities of heavy metals in soil, which persists there for
an indefinite period to have long lasting effects in the soil.
With increasing environmental awareness and legal
constraints being imposed on discharge of effluents,
different type of techniques presently in existence for
removal of heavy metals from contaminated waters
including reverse osmosis, electrodialysis, ion-exchange,
chemical precipitation, phytoremediation, etc. However,
all these methods have disadvantages like incomplete
metal removal, high reagent and energy requirements,
generation of toxic sludge or other waste products that
require careful disposal (Ahalya et al., 2003). Recently
research interest has diverted towards use of
4. 4
004 E3 J. Microbiol. Res
Table 2: Concentration of Heavy Metals in Kasur Soil (mg/kg)
Pb Cd Cr Cu Ni Zn
Min 42.1 2.0 54.1 31.2 32.6 81.4
Max 70.2 3.4 210.2 60.8 56.6 156.7
Mean 50.6 2.6 99.6 44.6 46.4 125.6
Median 47.2 2.6 93.2 43.9 50.0 131.4
S.D ±10.0 ±0.5 ±49.4 ±8.3 ±8.2 ±28.4
Kurtosis 0.852364 -0.95102 4.182443 2.326493 -0.60081 -1.26948
Skewness 1.285644 0.437593 1.824419 0.578925 -0.75136 -0.57778
Table 3: Tolerance index of fungal isolates against lead.
Isolated fungus Pb (TI) Isolated fungus Pb (TI)
Aspergillus fumigatus(K3) 0.29 Aspergillus fumigatus(K26) 0.28
Aspergillus fumigatus (K4) 0.30 Aspergillus fumigatus(K27) 0.17
Aspergillus flavus (K 5) 0.47 Aspergillus fumigatus (K28) 0.23
Aspergillus flavus (K 7) 1.09 Aspergillus fumigatus (K29) 0.28
Aspergillus niger(K 14) 0.71 Aspergillus fumigatus (K30) 0.25
Aspergillus niger (K 16) 0.48 Aspergillus flavus (K 33) 0.89
Aspergillus flavus (K 17) 1.18 Aspergillus flavus (K 34) 1.26
Aspergillus niger (K 22) 0.62 Aspergillus flavus (K 36) 0.12
Aspergillus niger (K 23) 0.54 Aspergillus flavus (K 35) 0.14
Aspergillus niger(K 24) 0.63 Aspergillus flavus (K 37) 0.39
Aspergillus flavus (K 41) 0.55
biomaterials like microbial biomass has emerged as an
alternative technique.
Fungi are natural inhabitants of soil and have greater
potential for remediation by virtue of their aggressive
growth; greater biomass, production and extensive
hyphal reach in soil. They are a versatile group, as they
can adapt and grow under various extreme conditions of
pH, temperature and nutrient availability, as well as high
metal concentrations (Anand et al., 2006). They offer the
advantage of having cell wall material which shows
excellent metal-binding properties (Gupta et al., 2000).
Describing the ability to grow at high metal
concentrations, (Malik, 2004) distinguished fungi tolerant
to heavy metals. Generally, the contaminated sites are
the sources of metal resistant micro-organism (Gadd,
1993).
The results of present study depicted that all tested
isolates shows different tolerance behavior for different
metals. Some isolates were sensitive, moderately tolerant
and tolerant (see table 2). Most of the isolates were
sensitive and very few were tolerant. Among all tested
isolates of Aspergillus, A.flavus was more tolerant specie
while A.niger was moderately tolerant. As Baldrian and
Gabriel, 2002 reported that various genera and also
isolates of the same genus did not necessarily have the
same heavy metal tolerance.
It is apparent that these fungal strains exhibit
considerable tolerance towards Cr and Pb and can
become dominant microorganisms in some polluted
habitats. Aspergillus .flavus and Aspergillus niger showed
tolerance against Pb but it showed sensitiveness for
Chromium metal. On the other hand, A.fumigtus shows
exactly contrasting behavior.
This response (i.e. resistance to Cr and Pb) of
Aspergillus flavus can be exploited for the remediation of
heavy metal contaminated soil (McGrath, 2002).
The variation in the metal tolerance may be due to the
presence of one or more strategies of tolerance or
resistance mechanisms exhibited by fungi (Ezzouhri et al.,
2009). The level of resistance depended on the isolate
5. 5
Shazia et al. 005
(A)
(B)
Figure 2: Tolerance index of isolates of A.fumigatus against (a) chromium and (b) lead
tested as well as the site of its isolation. The statistical
analysis showed the diverseness in heavy metal
tolerance of different isolates (see Figures 2, 3 and 4).
Fungi growth phase, which was observed from the
tolerance index of the fungi with time upon exposure to
heavy metals, was found to be a predictable pattern for
each strain and metal concentrations. The determination
of Minimum inhibitory concentration (MIC) of metals
suggests that the resistance level against individual
metals dependent on different fungal isolates. MIC was
defined as the minimum inhibitory concentration of the
heavy metal that inhibited visible growth of test fungi.
MIC of Cr and Pb is determined. It ranged from
800mg/l to 1000mg/l for chromium and lead for all
tested isolates (figure 3). The growth phase was
characterized by a lag, retarded, similar and enhanced
rates compared to control which appear to reflect the
tolerance development or adaptation of fungi in the
presence of heavy metals (Hashem et al., 1995).
The results revealed that growth rate of all tested
isolates at lower concentration was higher but with but
with passage of time and exposure of higher
concentration density and growth of species were
reduced leading to no growth. It was of interest to
establish the effect of type of heavy metal, the metal
concentration and the strain on this adaptive behavior.
The same increment and reduction in growth was observed
during study on filamentous fungi, which belonged to the
genera Aspergillus, were more resistant to Cr at higher
metal concentrations and suddenly the growth pattern
changes (Ngodigha, 1999)
El-Morsy (2004) studied 32 fungal species isolated from
polluted water in Egypt for their resistance to metals and
found that Cunninghamela echinulata biomass could be
employed as a biosorbent of metal ions in wastewater.
However, although some authors found that micro-
organisms isolated from contaminated sites were more
tolerant than those from natural environments (Ngodigha et
al., 1999; Verma et al., 2001).
0
0.4
0.8
1.2
k4 k3 k27 k28 k26 k29 k30
Toleranceindex
7. 7
Shazia et al. 007
(A)
(B)
Figure 4: Tolerance index of isolates of A.niger against (a) chromium and (b) lead
Figure 5:Minimum inhibitory concentration of different species of Aspergillus
against metals
Our preliminary findings indicate that fungi from soil
contaminated with heavy metals have tolerance potential
due to their physiological adaptation and they have
greater potential for remediation of metals. A.flavus and
A.niger showed higher tolerance potential than other
tested fungal strain which makes them more attractive
potential candidates for further investigations regarding
their ability to remove metals from contaminated sites.
Conclusion
The present study concludes that soil irrigated with waste
water contains tolerant fungus. The findings are A.flavus
and A.niger showed higher tolerance potential than other
tested fungal strain which makes them more attractive
potential candidates. It is affirmed that the response of
the isolates to heavy metals depended on the metal
0
0.2
0.4
0.6
0.8
K16 K22 k23 K14 K24
Toleranceindex
8. 8
008 E3 J. Microbiol. Res
tested; its concentration in the medium and on the isolate
considered and can be used further for the purposes of
bioremediation.
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