This document outlines how to implement the 5S methodology to organize and improve efficiency in a laboratory environment. The 5S system includes five phases: Sort, Set in Order, Shine, Standardize, and Sustain. Benefits of 5S include increased safety, employee involvement, organization, cleanliness, and space savings. Key aspects of implementation involve sorting necessary from unnecessary items, determining locations for necessary items, developing standard operating procedures, establishing performance measures, and sustaining practices through daily checklists and audits. Successful 5S requires commitment from all laboratory personnel.