These are the slides of the keynote talk in the science communication session, at the Plant Biology Europe FESPB/EPSO Congress, 24 June 2014 in Dublin (http://europlantbiology.org/).
3 Things Every Sales Team Needs to Be Thinking About in 2017Drift
Thinking about your sales team's goals for 2017? Drift's VP of Sales shares 3 things you can do to improve conversion rates and drive more revenue.
Read the full story on the Drift blog here: http://blog.drift.com/sales-team-tips
An immersive workshop at General Assembly, SF. I typically teach this workshop at General Assembly, San Francisco. To see a list of my upcoming classes, visit https://generalassemb.ly/instructors/seth-familian/4813
I also teach this workshop as a private lunch-and-learn or half-day immersive session for corporate clients. To learn more about pricing and availability, please contact me at http://familian1.com
3 Things Every Sales Team Needs to Be Thinking About in 2017Drift
Thinking about your sales team's goals for 2017? Drift's VP of Sales shares 3 things you can do to improve conversion rates and drive more revenue.
Read the full story on the Drift blog here: http://blog.drift.com/sales-team-tips
An immersive workshop at General Assembly, SF. I typically teach this workshop at General Assembly, San Francisco. To see a list of my upcoming classes, visit https://generalassemb.ly/instructors/seth-familian/4813
I also teach this workshop as a private lunch-and-learn or half-day immersive session for corporate clients. To learn more about pricing and availability, please contact me at http://familian1.com
The Purpose Economy: Business Principles for the Post-Capitalist SocietyDavid Fossas
This presentation explores key trends and principles that business leaders, and especially marketers, should be thinking about. The story came about when I started asking myself "What's next?" With technologies and sciences converging, it's hard to conceive the pace of innovation slowing. If anything, it will continue to accelerate, and we'll see a new wave of technological revolutions advance our society. While I don't make any predictions about what that next wave will be, I do share some thoughts on how we can (1) anticipate the timing, and (2) how business leaders can adapt to this shifting landscape.
When Karma Won't Cut It: Disrupting the Status Quo as a Woman (or Male Advoc...PayScale, Inc.
An examination of what's truly driving gender inequity and the gender pay gap in the workplace, why companies should be committed to addressing it and how women working in tech can advance their own careers (and salaries!) while we all wait for the world to change.
LinkedIn: Home to the Global Business EliteLinkedIn
The latest round of the Global Business Elite survey shows over a third of the world’s most influential and affluent people using LinkedIn at least once a month.
The research shows the same level of penetration for LinkedIn amongst the highest-level C-Suite executives, 118,000 of whom use the network daily. And the platform also reaches 58% of international business travellers.
Top business users are amongst LinkedIn’s most engaged and digitally savvy members, with 83% accessing content via a smartphone. And with high personal net worth and big business-purchase budgets, they represent the most valuable consumers on the planet.
http://nextberlin.eu/2012/08/elisabeth-stangl-on-the-creative-track/
Where is the future of the digital creative agency in times where their core business is disappearing? How can creatives provide more value for a brand in a world where the boundaries between physical and digital are being blurred. How can creatives spearhead innovation and pioneer brand communication? How does it affect their business models? Is there a post digital age of advertising? The creative track is designed to inspire and to deliver best of case studies in a world where idea is king and digital is just a technique. We will meet some of the most influential inventors of our post digital lives, see how a co-creation platform from China can inspire the world and invite ‘the new creators’ to present their tools for a post-digital future.
تقرير بحثي: مواقف مستخدمي الإنترنت في منطقة الشرق الأوسط حيال السلامة والأمن ...MOTC Qatar
قامت وزارة الاتصالات وتكنولوجيا المعلومات بتدشين النتائج الرئيسية لدراسة جديدة تمت على مستوى منطقة الشرق الأوسط وشمال أفريقيا وتناولت توجهات وسلوكيات مستخدمي الإنترنت
النتائج تم التوصل إليها باستخدام عينة بحثية قوامها 2793 من مستخدمي الإنترنت في المنطقة، تم مقارنة إجاباتهم بتلك البيانات المستخلصة من دراسات قام بها المنتدى الاقتصادي العالمي مستخدماً عينة من 8432 موزعين على 44 دولة من حول العالم
تمت الدراسة بالمشاركة مع "معهد أكسفورد للإنترنت" التابع لجامعة أكسفورد، و "كلية صمويل كيرتس جونسون العليا للإدارة" التابعة لجامعة كورنيل. العمل الميداني تم من خلال اثنين من الشركات العالمية التي تقود دراسات السوق: "كومسكور" و " تولونا
From the Stem Cell workshop at the Bio-Link Summer Fellows Forum 2011
presenters: Edie Kaeuper, Carin Zimmerman, Golnar Afshar, Samantha Croft, & Bill Woodruff
Evolutionary bio essay for final
Statement Of Purpose For Molecular Biology
The Bioecological Model of Human Development
Essay On Biotechnology
Biology Is The Science Of Life
Ap Biology Reflection
Why I Chose Biology
Bio 101 Essay
Biofuels Essay
Bio Ethics
Evolutionary Biology Essay
Biological Aspects Of Biological Anthropology
Reflection Paper On Bioethics
BioEthics Essay example
Bio Engineered Foods Essay
Biotechnology Essay
The Biopsychosocial Model
Biology : A Career In The Career Of Biology
The Biopsychosocial Model Essay
My Biography Essay
Scholarly video journals to increase productivity in research and educationNASIG
The biological and medical research chronically suffers from the low reproducibility of experimental studies since the traditional text format of science journals cannot provide an adequate description of details and nuances of complex experimental procedures. This creates a critical “bottleneck” problem of knowledge transfer for research and education. Addressing this challenge, a new generation of science journals employs video online to provide a systematic visualized publication of experimental studies. Visualization through video greatly facilitates the understanding and learning of experimental procedures enhancing knowledge transfer and productivity in research and education. The video-based publication in the rigorous academic format requires a new set of concepts and technical approaches to production, publication and dissemination processes. As the publisher of Journal of Visualized Experiments (JoVE), the first video journal for biological sciences indexed in MEDLINE and PubMed, I would like to provide an overview of the growing field of video publication and discuss its technical challenges, implications for scholarly communication and acceptance in the academic and library community.
Presenters: Moshe Pritsker, JoVE
Now available a new issue !!
Hysteroscopy and cystic adenomyosis, interview with Prof. Osama Shawki, Tamoxifen and hysteroscopy, Postmenstrual vaginal bleeding, endoscopycongress, new devices, and much more
The Purpose Economy: Business Principles for the Post-Capitalist SocietyDavid Fossas
This presentation explores key trends and principles that business leaders, and especially marketers, should be thinking about. The story came about when I started asking myself "What's next?" With technologies and sciences converging, it's hard to conceive the pace of innovation slowing. If anything, it will continue to accelerate, and we'll see a new wave of technological revolutions advance our society. While I don't make any predictions about what that next wave will be, I do share some thoughts on how we can (1) anticipate the timing, and (2) how business leaders can adapt to this shifting landscape.
When Karma Won't Cut It: Disrupting the Status Quo as a Woman (or Male Advoc...PayScale, Inc.
An examination of what's truly driving gender inequity and the gender pay gap in the workplace, why companies should be committed to addressing it and how women working in tech can advance their own careers (and salaries!) while we all wait for the world to change.
LinkedIn: Home to the Global Business EliteLinkedIn
The latest round of the Global Business Elite survey shows over a third of the world’s most influential and affluent people using LinkedIn at least once a month.
The research shows the same level of penetration for LinkedIn amongst the highest-level C-Suite executives, 118,000 of whom use the network daily. And the platform also reaches 58% of international business travellers.
Top business users are amongst LinkedIn’s most engaged and digitally savvy members, with 83% accessing content via a smartphone. And with high personal net worth and big business-purchase budgets, they represent the most valuable consumers on the planet.
http://nextberlin.eu/2012/08/elisabeth-stangl-on-the-creative-track/
Where is the future of the digital creative agency in times where their core business is disappearing? How can creatives provide more value for a brand in a world where the boundaries between physical and digital are being blurred. How can creatives spearhead innovation and pioneer brand communication? How does it affect their business models? Is there a post digital age of advertising? The creative track is designed to inspire and to deliver best of case studies in a world where idea is king and digital is just a technique. We will meet some of the most influential inventors of our post digital lives, see how a co-creation platform from China can inspire the world and invite ‘the new creators’ to present their tools for a post-digital future.
تقرير بحثي: مواقف مستخدمي الإنترنت في منطقة الشرق الأوسط حيال السلامة والأمن ...MOTC Qatar
قامت وزارة الاتصالات وتكنولوجيا المعلومات بتدشين النتائج الرئيسية لدراسة جديدة تمت على مستوى منطقة الشرق الأوسط وشمال أفريقيا وتناولت توجهات وسلوكيات مستخدمي الإنترنت
النتائج تم التوصل إليها باستخدام عينة بحثية قوامها 2793 من مستخدمي الإنترنت في المنطقة، تم مقارنة إجاباتهم بتلك البيانات المستخلصة من دراسات قام بها المنتدى الاقتصادي العالمي مستخدماً عينة من 8432 موزعين على 44 دولة من حول العالم
تمت الدراسة بالمشاركة مع "معهد أكسفورد للإنترنت" التابع لجامعة أكسفورد، و "كلية صمويل كيرتس جونسون العليا للإدارة" التابعة لجامعة كورنيل. العمل الميداني تم من خلال اثنين من الشركات العالمية التي تقود دراسات السوق: "كومسكور" و " تولونا
From the Stem Cell workshop at the Bio-Link Summer Fellows Forum 2011
presenters: Edie Kaeuper, Carin Zimmerman, Golnar Afshar, Samantha Croft, & Bill Woodruff
Evolutionary bio essay for final
Statement Of Purpose For Molecular Biology
The Bioecological Model of Human Development
Essay On Biotechnology
Biology Is The Science Of Life
Ap Biology Reflection
Why I Chose Biology
Bio 101 Essay
Biofuels Essay
Bio Ethics
Evolutionary Biology Essay
Biological Aspects Of Biological Anthropology
Reflection Paper On Bioethics
BioEthics Essay example
Bio Engineered Foods Essay
Biotechnology Essay
The Biopsychosocial Model
Biology : A Career In The Career Of Biology
The Biopsychosocial Model Essay
My Biography Essay
Scholarly video journals to increase productivity in research and educationNASIG
The biological and medical research chronically suffers from the low reproducibility of experimental studies since the traditional text format of science journals cannot provide an adequate description of details and nuances of complex experimental procedures. This creates a critical “bottleneck” problem of knowledge transfer for research and education. Addressing this challenge, a new generation of science journals employs video online to provide a systematic visualized publication of experimental studies. Visualization through video greatly facilitates the understanding and learning of experimental procedures enhancing knowledge transfer and productivity in research and education. The video-based publication in the rigorous academic format requires a new set of concepts and technical approaches to production, publication and dissemination processes. As the publisher of Journal of Visualized Experiments (JoVE), the first video journal for biological sciences indexed in MEDLINE and PubMed, I would like to provide an overview of the growing field of video publication and discuss its technical challenges, implications for scholarly communication and acceptance in the academic and library community.
Presenters: Moshe Pritsker, JoVE
Now available a new issue !!
Hysteroscopy and cystic adenomyosis, interview with Prof. Osama Shawki, Tamoxifen and hysteroscopy, Postmenstrual vaginal bleeding, endoscopycongress, new devices, and much more
Presentation given at NUI, Galway 2019-04-11 for Open Science Week.
An overview of Early Career Researchers, their innovation and contribution towards Open Infrastructure
Utilisation of non-edible, renewable lignocellulosic biomass for the production of second generation biofuels and chemicals is hindered especially by the high price of enzymes needed for biomass degradation. Filamentous fungi are natural producers of enzymes active against plant cell wall polymers. Especially the ascomycota fungus Trichoderma reesei is widely utilised in the industry for the production of cellulases and hemicellulases. However, the efficiency of enzyme production needs to be further improved in order to ensure economical production of biobased products. Several environmental factors affect protein production by filamentous fungi. Cellulase and hemicellulase genes of T. reesei are activated by inducer molecules derived from different substrates. The need for cooperation of different hydrolytic enzymes for the total degradation of plant cell wall material has led to coordinated expression of these genes. However, the extent and timing of induction can vary between different genes and especially the hemicellulase genes are differentially induced by various substrates. The direct regulation of cellulase and hemicellulase genes by transcriptional regulators has been widely studied and several activators and repressors of these genes have been characterized in detail. However, little is still known concerning the exact regulatory pathways and mechanisms utilised by the fungus for the accurate timing and composition of the hydrolytic enzymes produced.
In this study, a genome-wide transcriptional analysis of T. reesei gene expression at different ambient pH conditions was conducted in order to identify genes affected by extracellular pH. The role of a T. reesei orthologue for the characterized pH regulator, PacC, in the expression of cellulase and hemicellulase genes was also studied. An extensive induction experiment together with transcriptional profiling was then utilised to study the effects of several different substrates on the expression of genes encoding carbohydrate active enzymes (CAZy). In addition, transcriptomics data was utilised for the identification of novel candidate regulators affecting cellulase and xylanase production by T. reesei.
Transcriptional profiling identified pH as an important determinant of T. reesei gene expression. Ambient pH was also found to affect the expression of several cellulase and hemicellulase genes and more information on the role of a PacC orthologue in the expression of cellulase and hemicellulase genes was gained. A profiling study utilising different substrates as inducers together with a thorough annotation of the T. reesei CAZy genes revealed the expression patterns of novel candidate genes possibly involved in the degradation of different types of cellulosic and hemicellulosic substrates.
How can postdocs enhance their career opportunities? No matter what you end up doing, networking, flexibility and focusing on your progeny can help (just like plant meristems)
From a talk at Carnegie Institute at Stanford University 23 July 2014
This is my presentation on April 17, 2007 at the Chemical Heritage Foundation in Philadelphia, PA. I describe Open Notebook Science in the context of education.
These are the slides from a workshop that I ran for the student societies in the Department of Biological and Medical Sciences, Oxford Brookes University. They include 12 of the most important and useful LinkedIn features for students, such as adding multimedia to your profile, building your professional network, or discovering new jobs.
How can researchers use social platforms for dissemination and engagement?Anne Osterrieder
This presentation is aimed at researchers and other professionals in an academic environment who are either social media novices or have some knowledge but would like to learn more about expanding their online reach.
This is a presentation I gave at the annual meeting of the Society for Experimental Biology 2013 (http://www.sebiology.org/meetings/Valencia/Valencia.html). It contains lots of tips for scientists to use social media appropriately and efficiently. It also highlights examples of social media in academia and types of possible content.
These are the slides from a blogging workshop run by my colleague Marion Waite (Senior Lecturer and Brookes Teaching Fellow) and me, for the Oxford Brookes University Faculty for Health and Life Sciences Research Conference, 25 June 2013.
Making organelles visible - in planta and in societasAnne Osterrieder
This is the presentation I gave in Salzburg at the Annual Meeting of the Society for Experimental Biology, July 2012, for receiving the President's Medal for Education and Public Affairs.
http://www.sebiology.org/meetings/Salzburg2012/pres_meds.html
Enhancing your online presence with social mediaAnne Osterrieder
How can scientists use social media to enhance their online profile? Becoming pro-active and increasing your visibility is essential for your career development. Social media is a very useful tool to help you to get your name out there and to extend your professional network.
This is a talk which I gave on 2nd July in the "Advanced Communications" session at the SEB (Society for Experimental Biology) Annual Meeting, Salzburg 2012.
More information: http://www.sebiology.org/meetings/Salzburg2012/education.html
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
25. Why is this important?
Understand molecular mechanisms of Golgi stack structure
Understand Golgi populations in individual cells
Put Golgi populations into functional context in cells
Study effects on plant phenotypes
34. “I will never stand behind a lectern again! I will always need
to have a stage!”
“I used some of the theatre techniques for my presentation
[at a research conference] - standing with my feet apart,
breathing properly. It helped me.”
“I spend 50 hours per week in my office, not talking to
people. [A project like this] is a healthy thing to do if
you are doing an Arts and Humanities PhD.”
37. Your research
Discipline, research area or specific project?
General concepts or actual data?
Published or unpublished research?
Issues: Intellectual property, copyright,
collaborative research, peer review…
38. Who is your audience?
Peers Other researchers
Teachers Media
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Adults Young people
Colleagues
39. Your goals and preferences
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How much time do you have?
Do you prefer to write, take photos, speak…?
What do you enjoy doing?