Parámetros morfométricos y mercurio total en huevos de garceta nevada (Egretta thula) de la Bahía de Cartagena y ciénaga del Totumo , al norte de Colombia
abstract
Extracts of the medicinal plant Palicourea rigida Kunth, popularly known as douradinha, are
widely used for treating urinary tract disorders. Unfortunately, nowadays this is one of the
species endemic to Brazilian Cerrado that is at greatest risk of extinction.
The aim of the this work was to use AFLP molecular markers to determine the genetic
structure and diversity of eight natural populations of P. rigida and to associate their genetic
characteristics with loganin production in order to obtain provide relevant information
to promote programs for the conservation of this valuable medicinal plant.
A total of 120 polymorphic bands were scored and higher proportion of genetic diversity
was found in inter-populations (64%) rather than in intra-populations (36%). Fst value was
found to be significantly greater than zero (0.3601), demonstrating the complex genetic
structure of P. rigida populations. Accessions collected from Cristalina, GO, showed higher
percentage of polymorphic loci (65.5%) and the highest genetic diversity. Analysis of
Molecular variance (AMOVA) demonstrated 63.9% of intra-population genetic variation.
The lowest genetic variability was detected among accessions from the population found
in Sacramento, MG. No spatial standard was observed for P. rigida population, suggesting a
partially isolated island model. It was observed a minor but significant positive correlation
(r ¼ 0.22) between chemical and genetic matrices. The association between chemical and
genetic data indicated that environmental factors promoted the loganin production in
populations growing in Luziânia, GO, and therefore accessions from those populations
should be considered as prime material for initiating the conservation process of P. rigida.
2013 Elsevier Ltd. All rights reserved.
Near and mid-infrared spectroscopic determination of algal compositionzhenhua82
The objective of this study was to evaluate whether near-infrared reflectance spectroscopy (NIRS) or mid-infrared reflectance spectroscopy (MIRS) could be used to determine the composition of algal turf scrubber samples. We assayed a set of algal turf scrubber (ATS) samples (n = 117) by NIRS, MIRS, and conventional means for ash, total sugar, mono-sugar, total N, and P content. A subset of these samples (n = 64) were assayed by conventional means, MIRS, and NIRS for total lipid and total fatty acid content. We developed calibrations using all the samples and a one-out cross-validation procedure under partial least-squares regression. This process was repeated using 75% of randomly selected samples to develop the calibration and the remaining samples as an independent test set. Results using the entire sample set demonstrated that NIRS and MIRS can accurately determine ash (r (2) = 0.994 and 0.995, respectively) and total N (r (2) = 0.787 and 0.820, respectively) content, but not phosphorus, total sugar, or mono-sugar content in ATS samples. Results using the 64 sample subset indicated that neither NIRS nor MIRS can accurately determine lipid or total fatty acid content in ATS samples.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
In this study, the green-lipped mussels Perna viridis were collected from a high activity sampling at
Senibong in the Straits of Johore and two relatively clean sites with fi sh aquacultural activity at Bagan
Tiang (Perak) and Sg. Semerak (Kelantan). The mussels were dissected by gender into byssus, crystalline
style, foot, gill, gonad, mantle and muscle.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
abstract
Extracts of the medicinal plant Palicourea rigida Kunth, popularly known as douradinha, are
widely used for treating urinary tract disorders. Unfortunately, nowadays this is one of the
species endemic to Brazilian Cerrado that is at greatest risk of extinction.
The aim of the this work was to use AFLP molecular markers to determine the genetic
structure and diversity of eight natural populations of P. rigida and to associate their genetic
characteristics with loganin production in order to obtain provide relevant information
to promote programs for the conservation of this valuable medicinal plant.
A total of 120 polymorphic bands were scored and higher proportion of genetic diversity
was found in inter-populations (64%) rather than in intra-populations (36%). Fst value was
found to be significantly greater than zero (0.3601), demonstrating the complex genetic
structure of P. rigida populations. Accessions collected from Cristalina, GO, showed higher
percentage of polymorphic loci (65.5%) and the highest genetic diversity. Analysis of
Molecular variance (AMOVA) demonstrated 63.9% of intra-population genetic variation.
The lowest genetic variability was detected among accessions from the population found
in Sacramento, MG. No spatial standard was observed for P. rigida population, suggesting a
partially isolated island model. It was observed a minor but significant positive correlation
(r ¼ 0.22) between chemical and genetic matrices. The association between chemical and
genetic data indicated that environmental factors promoted the loganin production in
populations growing in Luziânia, GO, and therefore accessions from those populations
should be considered as prime material for initiating the conservation process of P. rigida.
2013 Elsevier Ltd. All rights reserved.
Near and mid-infrared spectroscopic determination of algal compositionzhenhua82
The objective of this study was to evaluate whether near-infrared reflectance spectroscopy (NIRS) or mid-infrared reflectance spectroscopy (MIRS) could be used to determine the composition of algal turf scrubber samples. We assayed a set of algal turf scrubber (ATS) samples (n = 117) by NIRS, MIRS, and conventional means for ash, total sugar, mono-sugar, total N, and P content. A subset of these samples (n = 64) were assayed by conventional means, MIRS, and NIRS for total lipid and total fatty acid content. We developed calibrations using all the samples and a one-out cross-validation procedure under partial least-squares regression. This process was repeated using 75% of randomly selected samples to develop the calibration and the remaining samples as an independent test set. Results using the entire sample set demonstrated that NIRS and MIRS can accurately determine ash (r (2) = 0.994 and 0.995, respectively) and total N (r (2) = 0.787 and 0.820, respectively) content, but not phosphorus, total sugar, or mono-sugar content in ATS samples. Results using the 64 sample subset indicated that neither NIRS nor MIRS can accurately determine lipid or total fatty acid content in ATS samples.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
In this study, the green-lipped mussels Perna viridis were collected from a high activity sampling at
Senibong in the Straits of Johore and two relatively clean sites with fi sh aquacultural activity at Bagan
Tiang (Perak) and Sg. Semerak (Kelantan). The mussels were dissected by gender into byssus, crystalline
style, foot, gill, gonad, mantle and muscle.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Observation for spoilage in fish and beef in a daily simulated local market s...IJEABJ
The degree of spoilage of fish and beef in a daily simulated market style of southwest, Nigeria was determined by Trimethylamine (TMA) levels in each sample using the standard pictrate technique. 100g of each of the three different parts of a bull (liver, meaty and fatty tissues) and fresh African cat fish (Clarias gariepinus) (liver, meaty portion and head) were purchased twice a week for five weeks from the abattoir and Oja-Oba market in Akure respectively. The samples were subjected to ambient temperature and their degree of spoilage was assessed after 3 hours, 6 hours and 9 hours of purchase. The results showed that, there was no significant difference (P≥ 0.05) in the effect of time (hours) on TMA concentration in different parts of the fish and bull samples, though, the mean concentration of TMA in the fish samples increased with time. However, the degree of spoilage is slower in the bull compared to the fish samples but higher in the late evening (9 hours). Hence, buying and selling of beef in the late evening should be discouraged to avoid consumption of unwholesome meat with high TMA concentrations, while fish should be stored-frozen and sold in deep freezers.
Hematological Parameters of three Strains of Local Cocks in Northern NigeriaIJEAB
The study was conducted to determine the hematological parameters of three strains of the Nigerian indigenous cocks. A total of 15 sexually matured (14-18 month of age) breeders cocks comprising (5 normal feathered, 5 frizzled feathered and 5 naked neck) were used for the experiment. The study was conducted from October to December 2016 at the Teaching and Research Farm University of Maiduguri. Blood samples were collected from 9 breeder’s cocks which were randomly selected 3 per genotype and used for hematological parameters examination. Hematological examination such as Packed Cell Volume (PCV), Red Blood Cell ( RBC) , Haemoglobin (Hb), White Blood Cell (WBC), Mean Corpuscular Haemoglobin concentration ( MCHC), Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Volume ( MCV) showed significant (P<0.05)> 0.05) different between normal feathered and frizzle feathered but there is significant difference ( P< 0.05) with naked necked cock. Neutrophil ( N) showed significant (P<0.05)>0.05) difference between normal feathered and naked neck feathered , fizzle feathered and necked neck respectively but showed significant (P<0.05) difference between fizzle feathered and normal feathered respectively for M and E. the study concluded that variation in the heamatoloical parameters between three strains of local chicken in Nigeria is due to difference in their genetic makeup.
E-screen assay validation: evaluation of estrogenic activity by MCF7 cell cul...Agriculture Journal IJOEAR
— Natural and synthetic estrogens have been detected in rivers, lakes and estuaries in many parts of the world. Primary sources of these compounds are domestic and industrial effluents, which are not deleted after the water treatment. Estrogen has been the endocrine disruptor most researched to be very active biologically and be the etiologic agent of diverse types of cancer and other conditions such as endometriosis, precocious puberty, feminization, masculinization, sterility. In this context, we use water of 36 natural reservoirs or dams, in a bioassay to characterize their estrogenicity in culture of MCF7 cells and obtained high concentration of estrogen in samples taken in Ibiúna and Equestrian Santo Amaro / SP. However, certain concentration in our samples for most water samples from different regions was very close to the limit of quantification by bioassay and estrogen was in fmol. It has been shown that e-screen assay with MCF7 cells is a sensitive and stable tool for quantitative analysis of estrogenicity of water and can easily be developed and implemented for routine for estrogen quantification also in animal food and man, aqueous and plastics etc. Keywords— endocrine disrupters, estrogen, breast cancer cells, (MCF7) bioassay: E-screen assay
The Growth of Oreochromis niloticus (2.6g initial average size) was studied in 100m2 earthen ponds for 180 days under tropical field environment. Varying fertilizing regimes consisting of Pennisetum purpureum (PP) and Musa sapientum (MS), two locally available weedy grasses were applied in duplicates in the experimental ponds, at the rate of 0.1-0.2 kg dry matter per m2 per day. Water quality parameters were monitored bimonthly between 8:00am-9:00am . Final average fish weight varied significantly (p<0.05) as follows: 43.85g, 35.5g, 59.5g, 24.9g and 50g, 2 respectively for PP, MS, MS+PP, T0 and T1. Corresponding daily growth were 2.2g/d, 0.1g/d, 0.3g/d, 0.1g/d and 0.2g/d. Survival rate equally varied significantly as follow: 60 %, 67 %, and 98 %, 70 % and 97 % respectively. During the study period, the results indicated the water quality were permissibole limits and can be used for livestock of Oreochromis niloticus .
DOI: 10.21276/ijlssr.2016.2.3.13
ABSTRACT- The present investigation was carried out to the effect of Fenvalerate on haematological parameters in
freshwater fish Channa marulius using standard methods. Fish was exposed to (1/4th LC50) sub lethal concentration of
Fenvalerate (0.086ppm) for 96h.The blood sample were obtained from both, control and experimental fish and assayed
haematological parameters (Total Erythrocytes count, TEC; Total Leucocytes count, TLC; Haemoglobin, Hb; Packed cell
volume, PCV; Mean corpuscular volume, MCV; Mean Corpuscular Haemoglobin and Mean Corpuscular Haemoglobin
Concentration; MCHC). The result revealed that TEC, Hb percentage, PCV and MCHC counts were significantly
decreased, whereas TLC, MCV and MCH increased slightly in experimental fish. The study has thus indicated marked
changes in blood of Channa marulius after exposure to Fenvalerate.
Key-words- Fenvalerate, Hematology, Channa marulius, Haemoglobin, Sub lethal
Analysis of Herbicide Atrazine and Its Degradation Products in Agricultural S...Agriculture Journal IJOEAR
Abstract— A novel ultra-performance liquid chromatography‒mass spectrometry (UPLC‒MS) method was developed for the determination of herbicide atrazine (ATR) and its principal metabolites namely deisopropylatrazine (DIA), deethylatrazine (DEA) and hydroxyatrazine (HA) in soils. The limit of detection ranged from 0.06 μg kg‒1 (DEA) to 0.25 μg kg‒1 (HA). Recoveries for the four target analytes at three spiked levels ranged from 73.2 to 110% with relative standard deviation of 5.1‒8.1%. In the cases of the three control soil samples spiked with ATR were treated for 60d, the sum content of the three degraded products is 3, 6.4, and 6.8 times greater than ATR residue, respectively. Analyzing 80 soil samples from four counties evaluated this method. ATR of 1.1‒125 μg kg‒1 in 80 of 80 samples, ATR of 0.5‒7.8 μg kg‒1 in 39 of 80 samples, and DIA of 0.5 and 0.6 μg kg‒1 in 2 of 80 samples were found. The proposed method can ensure the rapid and highly sensitive analysis of atrazine and its degradation products in soil, and can provide a direction for proper application of atrazine and a base for evaluating their hazards to the environment.
2017 - Environmental Ordination of Filamentous Bacteria in Activated SludgeWALEBUBLÉ
Reference:
Zornoza, A., Serrano, S. and Alonso, J.L. (2017) Environmental Ordination of Filamentous Bacteria in Activated Sludge. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
The main purpose of this paper was to investigate an experimental and theoretical analysis of
heat and moisture transport behavior in wood during convective drying process. A convective drying
cell was used to follow the measurements of the water content of samples subject to hot air flow in
longitudinal and transverse moisture transfer of wood. The effects of drying conditions such as
drying air temperature, air velocity and ambient relative humidity on the drying characteristics of
wood has been investigated. The constants drying and diffusion coefficients of the drying model,
which control the drying rate of wood, were determined from fitting the model against the
experimental drying curves. Results showed that, drying kinetic behaviour of the longitudinal
diffusion is very significant than the transverse one. The moisture content increased with increase in
drying air temperature and air velocity but decreased with time. From the curves of moisture flow
evolution versus moisture ratio, convective heat and mass transfer coefficients have been evaluated
and compared with values obtained from the literature and existing correlations.
Length-weight relationship, condition factor and proportionality index of two...Innspub Net
The length-weight relationship, condition factor and proportionality index of Callinectes amnicola and Cardisoma armatum from Ebrié Lagoon, Grand-Bassam, Côte d’Ivoire, were studied. The carapace width and length were measured to the nearest millimeter (mm) while weight was measured to the nearest grams (g). The length-weight relationship of all samples collected were determined for the various sexes and mixed populations. The values of the exponent ‘b’ for all the sexes and mixed populations ranged from 2.01-2.69. The growth generally exhibited negative allometry in all the sexes and mixed populations. The proportionality index showed that Cardisoma armatum was longer and Callinectes amnicola was wider.
Observation for spoilage in fish and beef in a daily simulated local market s...IJEABJ
The degree of spoilage of fish and beef in a daily simulated market style of southwest, Nigeria was determined by Trimethylamine (TMA) levels in each sample using the standard pictrate technique. 100g of each of the three different parts of a bull (liver, meaty and fatty tissues) and fresh African cat fish (Clarias gariepinus) (liver, meaty portion and head) were purchased twice a week for five weeks from the abattoir and Oja-Oba market in Akure respectively. The samples were subjected to ambient temperature and their degree of spoilage was assessed after 3 hours, 6 hours and 9 hours of purchase. The results showed that, there was no significant difference (P≥ 0.05) in the effect of time (hours) on TMA concentration in different parts of the fish and bull samples, though, the mean concentration of TMA in the fish samples increased with time. However, the degree of spoilage is slower in the bull compared to the fish samples but higher in the late evening (9 hours). Hence, buying and selling of beef in the late evening should be discouraged to avoid consumption of unwholesome meat with high TMA concentrations, while fish should be stored-frozen and sold in deep freezers.
Hematological Parameters of three Strains of Local Cocks in Northern NigeriaIJEAB
The study was conducted to determine the hematological parameters of three strains of the Nigerian indigenous cocks. A total of 15 sexually matured (14-18 month of age) breeders cocks comprising (5 normal feathered, 5 frizzled feathered and 5 naked neck) were used for the experiment. The study was conducted from October to December 2016 at the Teaching and Research Farm University of Maiduguri. Blood samples were collected from 9 breeder’s cocks which were randomly selected 3 per genotype and used for hematological parameters examination. Hematological examination such as Packed Cell Volume (PCV), Red Blood Cell ( RBC) , Haemoglobin (Hb), White Blood Cell (WBC), Mean Corpuscular Haemoglobin concentration ( MCHC), Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Volume ( MCV) showed significant (P<0.05)> 0.05) different between normal feathered and frizzle feathered but there is significant difference ( P< 0.05) with naked necked cock. Neutrophil ( N) showed significant (P<0.05)>0.05) difference between normal feathered and naked neck feathered , fizzle feathered and necked neck respectively but showed significant (P<0.05) difference between fizzle feathered and normal feathered respectively for M and E. the study concluded that variation in the heamatoloical parameters between three strains of local chicken in Nigeria is due to difference in their genetic makeup.
E-screen assay validation: evaluation of estrogenic activity by MCF7 cell cul...Agriculture Journal IJOEAR
— Natural and synthetic estrogens have been detected in rivers, lakes and estuaries in many parts of the world. Primary sources of these compounds are domestic and industrial effluents, which are not deleted after the water treatment. Estrogen has been the endocrine disruptor most researched to be very active biologically and be the etiologic agent of diverse types of cancer and other conditions such as endometriosis, precocious puberty, feminization, masculinization, sterility. In this context, we use water of 36 natural reservoirs or dams, in a bioassay to characterize their estrogenicity in culture of MCF7 cells and obtained high concentration of estrogen in samples taken in Ibiúna and Equestrian Santo Amaro / SP. However, certain concentration in our samples for most water samples from different regions was very close to the limit of quantification by bioassay and estrogen was in fmol. It has been shown that e-screen assay with MCF7 cells is a sensitive and stable tool for quantitative analysis of estrogenicity of water and can easily be developed and implemented for routine for estrogen quantification also in animal food and man, aqueous and plastics etc. Keywords— endocrine disrupters, estrogen, breast cancer cells, (MCF7) bioassay: E-screen assay
The Growth of Oreochromis niloticus (2.6g initial average size) was studied in 100m2 earthen ponds for 180 days under tropical field environment. Varying fertilizing regimes consisting of Pennisetum purpureum (PP) and Musa sapientum (MS), two locally available weedy grasses were applied in duplicates in the experimental ponds, at the rate of 0.1-0.2 kg dry matter per m2 per day. Water quality parameters were monitored bimonthly between 8:00am-9:00am . Final average fish weight varied significantly (p<0.05) as follows: 43.85g, 35.5g, 59.5g, 24.9g and 50g, 2 respectively for PP, MS, MS+PP, T0 and T1. Corresponding daily growth were 2.2g/d, 0.1g/d, 0.3g/d, 0.1g/d and 0.2g/d. Survival rate equally varied significantly as follow: 60 %, 67 %, and 98 %, 70 % and 97 % respectively. During the study period, the results indicated the water quality were permissibole limits and can be used for livestock of Oreochromis niloticus .
DOI: 10.21276/ijlssr.2016.2.3.13
ABSTRACT- The present investigation was carried out to the effect of Fenvalerate on haematological parameters in
freshwater fish Channa marulius using standard methods. Fish was exposed to (1/4th LC50) sub lethal concentration of
Fenvalerate (0.086ppm) for 96h.The blood sample were obtained from both, control and experimental fish and assayed
haematological parameters (Total Erythrocytes count, TEC; Total Leucocytes count, TLC; Haemoglobin, Hb; Packed cell
volume, PCV; Mean corpuscular volume, MCV; Mean Corpuscular Haemoglobin and Mean Corpuscular Haemoglobin
Concentration; MCHC). The result revealed that TEC, Hb percentage, PCV and MCHC counts were significantly
decreased, whereas TLC, MCV and MCH increased slightly in experimental fish. The study has thus indicated marked
changes in blood of Channa marulius after exposure to Fenvalerate.
Key-words- Fenvalerate, Hematology, Channa marulius, Haemoglobin, Sub lethal
Analysis of Herbicide Atrazine and Its Degradation Products in Agricultural S...Agriculture Journal IJOEAR
Abstract— A novel ultra-performance liquid chromatography‒mass spectrometry (UPLC‒MS) method was developed for the determination of herbicide atrazine (ATR) and its principal metabolites namely deisopropylatrazine (DIA), deethylatrazine (DEA) and hydroxyatrazine (HA) in soils. The limit of detection ranged from 0.06 μg kg‒1 (DEA) to 0.25 μg kg‒1 (HA). Recoveries for the four target analytes at three spiked levels ranged from 73.2 to 110% with relative standard deviation of 5.1‒8.1%. In the cases of the three control soil samples spiked with ATR were treated for 60d, the sum content of the three degraded products is 3, 6.4, and 6.8 times greater than ATR residue, respectively. Analyzing 80 soil samples from four counties evaluated this method. ATR of 1.1‒125 μg kg‒1 in 80 of 80 samples, ATR of 0.5‒7.8 μg kg‒1 in 39 of 80 samples, and DIA of 0.5 and 0.6 μg kg‒1 in 2 of 80 samples were found. The proposed method can ensure the rapid and highly sensitive analysis of atrazine and its degradation products in soil, and can provide a direction for proper application of atrazine and a base for evaluating their hazards to the environment.
2017 - Environmental Ordination of Filamentous Bacteria in Activated SludgeWALEBUBLÉ
Reference:
Zornoza, A., Serrano, S. and Alonso, J.L. (2017) Environmental Ordination of Filamentous Bacteria in Activated Sludge. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
The main purpose of this paper was to investigate an experimental and theoretical analysis of
heat and moisture transport behavior in wood during convective drying process. A convective drying
cell was used to follow the measurements of the water content of samples subject to hot air flow in
longitudinal and transverse moisture transfer of wood. The effects of drying conditions such as
drying air temperature, air velocity and ambient relative humidity on the drying characteristics of
wood has been investigated. The constants drying and diffusion coefficients of the drying model,
which control the drying rate of wood, were determined from fitting the model against the
experimental drying curves. Results showed that, drying kinetic behaviour of the longitudinal
diffusion is very significant than the transverse one. The moisture content increased with increase in
drying air temperature and air velocity but decreased with time. From the curves of moisture flow
evolution versus moisture ratio, convective heat and mass transfer coefficients have been evaluated
and compared with values obtained from the literature and existing correlations.
A study on length weight relationships (lwr) and growth responses of major ca...
Similar to [23] morphometric parameters and total mercury in eggs of snowy egret (egretta thula) from cartagena bay and totumo marsh, north of colombia
Length-weight relationship, condition factor and proportionality index of two...Innspub Net
The length-weight relationship, condition factor and proportionality index of Callinectes amnicola and Cardisoma armatum from Ebrié Lagoon, Grand-Bassam, Côte d’Ivoire, were studied. The carapace width and length were measured to the nearest millimeter (mm) while weight was measured to the nearest grams (g). The length-weight relationship of all samples collected were determined for the various sexes and mixed populations. The values of the exponent ‘b’ for all the sexes and mixed populations ranged from 2.01-2.69. The growth generally exhibited negative allometry in all the sexes and mixed populations. The proportionality index showed that Cardisoma armatum was longer and Callinectes amnicola was wider.
Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...Nii Korley Kortei
Oyster mushroom slices (Pleurotus ostreatus) were exposed to ɣ-radiation as a pretreatment and
solar dried to investigate the influence of irradiation on drying kinetics. Processing conditions
included exposure of mushrooms to 0 kGy (control), 0.5 kGy, 1.0 kGy, 1.5 kGy and 2.0 kGy
of ɣ-radiation at a dose rate of 1.7 kGy/h and drying at a mean temperature of 53.2±6.4°C.
Experimental drying data were fitted to 5 thin layer drying models by non-linear regression.
Irradiation was observed to enhance the drying rate of mushroom slices, with higher doses
causing faster moisture removal. Drying characteristics of slices exposed to lower dosages were
best described by Page’s model (R2=0.9878, 0.9967, 0.9925 correspondingly for “control” (0.0
kGy), 0.5 and 1.0 kGy while the Diffusion model best fit the data for those exposed to higher
doses of radiation (R2=0.9938, 0.9890 for 1.5 and 2.0 kGy respectively). Deff ranged from 1.88
to 2.44 x 10-08 and increase from “control”, 0.5 kGy, 1.0 kGy, 1.5 kGy to 2.0 kGy. Irradiation
of mushrooms as a pretreatment for drying increases moisture diffusivity and drying rate with
higher doses having the most effect.
Evaluation of the oyster farming potential of the Cintra bay (southern Morocco)Origins publication
The bay of Cintra is a marine ecosystem located in the southern Moroccan Atlantic known for its
biological richness and therefore for its high fishery productivity. This bay was chosen as a new destination for the
development of aquaculture in the southern regions of Morocco. To highlight its potential in terms of oyster
farming, a first rearing trial of triploid cupped oysters (Crassostrea gigas) as well as parallel monitoring of the
phytoplankton population and ecological parameters were undertaken. The results obtained showed that the oysters
adapt well to the conditions of the new environment where mortality was negligible, or even absent after a month
of launching. In terms of biological performance, the growth of individuals is continuous during the annual cycle
and after eight months the weight could reach 30.5 g which is a minimum weight for marketing. The AFNOR and
Lawrence and Scott indices showed a good physiological state and a better commercial quality of the oysters. The
filling rate of these oysters, with an average weight of 39.7g when lifting, is very high according to the Lawrence and
Scott index (126.3) and the average AFNOR index (around 21.8) rank them in the “Special” category.
Morphological adaptation of P. canaliculata shell to the different ecosystems...Open Access Research Paper
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[23] morphometric parameters and total mercury in eggs of snowy egret (egretta thula) from cartagena bay and totumo marsh, north of colombia
1. Morphometric parameters and total mercury in eggs of snowy egret
(Egretta thula) from Cartagena Bay and Totumo Marsh, north of Colombia
Jesus Olivero-Verbel ⇑
, Diana Agudelo-Frias, Karina Caballero-Gallardo
Environmental and Computational Chemistry Group, Faculty of Pharmaceutical Sciences, Campus of Zaragocilla, University of Cartagena, Cartagena, Colombia
a r t i c l e i n f o
Keywords:
Aquatic ecosystem
Endocrine disruption
Reproduction
Bird
Egg
a b s t r a c t
Eggs from egrets (Egretta thula) were collected from Cartagena Bay and Totumo Marsh, two sites at the
north of Colombia with different pollution background, and measured their morphometric parameters
as well as total mercury (T-Hg) and calcium levels in eggshell. Statistically significant differences were
observed for egg weight and size between the two sampling locations. T-Hg and calcium concentrations
in eggshell were greater in eggs from Cartagena Bay, the industrial site, compared to Totumo Marsh, a
non-industrial location. The opposite was observed for eggshell thickness (3.6% less in the bay). Pearson
correlation analysis showed eggshell T-Hg negatively correlated with eggshell weight in eggs from the
marsh (R = À0.795, P < 0.006), but not from the bay (R = 0.387, P = 0.269), probably suggesting greater
susceptibility to Hg in birds from the non-polluted site. In short, results suggest eggs from E. thula at
Cartagena Bay have greater T-Hg concentrations and less eggshell thickness than those from Totumo
Marsh.
Ó 2013 Elsevier Ltd. All rights reserved.
1. Introduction
During the last decades the survival of several bird species has
been threatened by different environmental factors including glo-
bal warning, urbanization and pollution. Many environmental pol-
lutants have been recognized as endocrine disruptors with proven
adverse effects on reproduction, including organic molecules, and
heavy metals (Sugni et al., 2010). Among these chemicals, mercury
(Hg) is of particular concern due to its ubiquitous nature, toxicity,
and capacity to be transferred from one trophic level to the next,
being biomagnified through aquatic and terrestrial food webs
(Bryan et al., 2012; Haines et al., 2003; Dolbec et al., 2001), and a
cause for concern regarding breeding bird populations (Hargreaves
et al., 2011). Therefore, it represents a key factor for biodiversity
conservation, especially for endangered species (Ackerman et al.,
2012). This is particularly important in tropical coastal ecosystems,
as stated by the United Nations document ‘‘The Future We Want’’,
signed in Rio de Janeiro in June 2012 (Costa et al., 2012).
In the Caribbean region of Colombia, a tropical coastal environ-
ment, tourism is one of the main economical activities, and in cities
such as Cartagena, the natural surroundings definitively are an
added value. In this scenario, the snowy egret (Egretta thula) and
other bird species coexist in the urban area, taking advantage of
the remaining available green spots. E. thula is a small, snow-white
heron with a slim, pointed, black bill, yellow eyes, bright yellow
feet, and long, black legs. However, entropic pressure on these
and other bird species is a challenging problem, as it has been re-
ported that Cartagena Bay, the main water body in the city, has
pollution issues related to biological agents (Olivero et al.,
2005a), new contaminants (Olivero et al., 2005b), some of which
have been already reported in humans (Kannan et al., 2004), and
mercury (Olivero-Verbel et al., 2009; Carvajal et al., 2000), among
others.
Despite the fact that the snowy egret is one of the most abundant
bird species in Cartagena Bay, few data have been collected on the
biology, ecology and toxicology associated with these birds in this
area. However, considering that its diet is based on fish, shrimps,
and sometimes insects, it occupies a high level in the trophic chain,
making it susceptible to the accumulation of heavy metals and other
endocrine dysfunction agents. As in marine ecosystems, eggshell Hg
is a markerfor previous Hg exposure (Xu et al., 2011), the objective of
this research was to evaluate the presence of Hg and morphological
characteristics of the eggs of E. thula, as an indicator of the pollution
statusof the egrets,and thepossible implications in the survivalabil-
ity for this species in Cartagena Bay.
2. Materials and methods
2.1. Sample collection
Eggs from E. Thula were obtained from active nests in two
different locations at the north of Colombia: Cartagena Bay and
Totumo Marsh (Fig. 1) on May–September, 2005. The bay has been
0025-326X/$ - see front matter Ó 2013 Elsevier Ltd. All rights reserved.
http://dx.doi.org/10.1016/j.marpolbul.2013.01.013
⇑ Corresponding author. Tel.: +57 5 6698179; fax: +57 5 6698323.
E-mail addresses: jesusolivero@yahoo.com, joliverov@unicartagena.edu.co (J.
Olivero-Verbel).
Marine Pollution Bulletin 69 (2013) 105–109
Contents lists available at SciVerse ScienceDirect
Marine Pollution Bulletin
journal homepage: www.elsevier.com/locate/marpolbul
2. largely recognized as an ecosystem with Hg-related pollution
(Olivero-Verbel et al., 2009, 2008; Olivero et al., 2005a,b; Carvajal
et al., 2000), whereas the Totumo Marsh, a coastal marsh, located
approximately 50 km north from the bay, is considered a low-
polluted area, with no Hg inputs from industrial sources. At each
sampling point, 20 eggs were manually collected, taking one egg
from nests containing at least three of them. Eggs were maintained
under refrigeration until analysis, no more than 1 week after
collection.
2.2. Morphometric parameters
Once collected, eggs were carefully washed with distilled water
and dried at room temperature on paper towels. Egg length, and
width, in the middle of the length, was measured with a vernier
caliper with a resolution of ±0.05 mm (Helander et al., 2002). Total
egg and eggshell weights were measured using an analytic scale to
a ±0.01 g resolution. Eggshell thickness was recorded on at least
five randomly taken fractions using a digital micrometer with a
precision of ±0.001 mm (Helander et al., 2002).
2.3. Total mercury in the eggshells and egg content
Individual eggshell fragments were carefully washed with a
toothbrush and deionized water to remove any organic mem-
branes or remnants of albumen or yolk. These were allowed to
air dry for 24 h and stored in plastic bags until Hg analysis. In
the case of egg content, both egg white and yolk were homoge-
nized using a vortex, the product transferred to plastic vials and
kept at À20 °C until analysis. Total Hg (T-Hg) concentrations were
assessed using 150 mg of previously pulverized eggshell, or 60 mg
of egg content. Samples were pirolyzed at 800 °C and the vaporized
Hg was detected by a RA-915+ Zeeman Mercury Spectrometer
(Sholupov et al., 2004), and quantified using a calibration curve ob-
tained from certified standards. Calibration curves were considered
optimal if the value of R was equal or greater than 0.99. The accu-
racy of the method was quantified by analysis of blanks, and the
use of the certified sample DORM-2, dogfish muscle, from the Na-
tional Research Council of Canada. The measured concentration of
T-Hg in DORM-2 was 4.46 ± 0.25 lg T-Hg/g (certified value
4.47 ± 0.32 lg T-Hg/g). The precision of the assay was estimated
as the coefficient of variation for two independent measurement
values. In all cases, it was always below 10%.
2.4. Calcium concentration in the eggshells
The calcium contents of eggshell samples were measured by X-
ray fluorescence (XRF) as previously described (Leoni and Saitta,
1976; Tamponi et al., 2003). One-gram samples of E. thula eggshells
were pulverized and mixed with 14 g of powdered polypropylene
homopolymer (PP) unused resin. The mixture was homogenized
by manual shaking during 3 min, placed in a stainless steel mold,
and then put under hydraulic press at 230 °C and 100 psi of pres-
sure, obtaining a 6 cm diameter disk. Readings were performed
on a Philips PW 1480 fluorescence instrument equipped with a
Sc anode tube, glass of LiF200, and a flow detector, with analytic
software X40. Calibration curves were prepared with calcium car-
bonate in PP and reported values are the averages of at least five
determinations.
2.5. Statistics
The results were reported as the average ± SE of the mean. Sta-
tistical differences between sampling sites were carried out using
T-test, after examining the data for normality (Kolmogorov–Smir-
nov) and homoscedasticity (Barlett). Otherwise, variable transfor-
mations were performed. Relationships between variables were
assessed using Pearson correlations. In all cases, statistical signifi-
cance was set at P < 0.05 (Walpole et al., 2001).
3. Results
Length, width and total weights of eggs from E. thula nesting in
Totumo Marsh and Cartagena Bay are shown in Fig. 2. Eggs from
Fig. 1. Map of Colombia showing the geographic localization of sampling areas.
Totumo Marsh (TM) and Cartagena Bay (CB).
Fig. 2. Length (A), width (B) and total weight (C) of the eggs from Egretta thula collected in Totumo Marsh and Cartagena Bay.
106 J. Olivero-Verbel et al. / Marine Pollution Bulletin 69 (2013) 105–109
3. egrets living at Totumo Marsh presented greater length
(44.30 ± 0.38 mm) and weight (24.42 ± 0.41 g) than those from
Cartagena Bay (42.64 ± 0.32 mm and 21.68 ± 0.42 g, respectively).
No statistical differences in egg widths were observed for the sam-
pling sites.
Eggshell thickness and weights are presented in Fig. 3. Eggs
from Totumo Marsh have significantly greater eggshell thickness
(196 ± 2.21 lm) than those from Cartagena Bay (189 ± 2.43 lm),
with a mean difference of 3.6%. Although, in average, eggshell
weights from Totumo Marsh area were 2.7% greater than those
from Cartagena Bay, the differences were not significant.
T-Hg levels in eggshell, egg content, and total calcium concen-
trations in eggshell are presented in Fig. 4. Eggshells from Cartage-
na Bay had greater Hg and Calcium concentrations than those from
Totumo Marsh. In the case of Hg, detected levels were more than
twofold greater in the bay. However, T-Hg concentrations obtained
in egg contents did not show statistically differences between sam-
pling sites.
Correlation analysis between different studied morphometric
and chemical parameters for eggs from E. thula are presented in
Table 1. Results showed associations between studied variables
varied depending on the sampling site. Interestingly, in Cartagena
Bay, all morphometric parameters were significantly correlated. In
contrast, in Totumo Marsh, significant correlations were found for
egg weight, length and width, but not between these variables and
eggshell weight or thickness. In the bay, a significant positive asso-
ciation was found between T-Hg in egg content and egg width
(R = 0.455, P = 0.044). In the marsh, T-Hg in eggshell inversely cor-
related with eggshell weight (R = À0.795, P = 0.006), and T-Hg in
egg content also negatively correlated with eggshell thickness
Fig. 3. Thickness (A) and weight (B) in eggshells of Egretta thula from Totumo Marsh and Cartagena Bay.
Fig. 4. Total mercury concentrations in eggshell (A), egg content (B), and total calcium in eggshell (C) of Egretta thula from Totumo Marsh and Cartagena Bay.
Table 1
Pearson correlation for mercury content and morphometric variables studied in eggs from Egretta thula living at Totumo Marsh (left side) and Cartagena Bay (right side,
underlined).
Parameters Egg weight Egg length Egg width Eggshell weight Eggshell thickness T-Hg in eggshell T-Hg in egg content
Egg weight 1 0.743 (P 6 0.001) 0.912 (P 6 0.001) 0.793 (P < 0.001) 0.523 (P = 0.018) 0.063 (P = 0.863) 0.365 (P = 0.114)
Egg length 0.772 (P < 0.001) 1 0.677 (P = 0.001) 0.756 (P < 0.001) 0.555 (P = 0.011) 0.020 (P = 0.957) 0.137 (P = 0.564)
Egg width 0.840 (P < 0.001) 0.475 (P = 0.035) 1 0.826 (P < 0.001) 0.518 (P = 0.019) 0.002 (P = 0.995) 0.455 (P = 0.044)
Eggshell weight 0.236 (P = 0.317) 0.099 (P = 0.677) 0.251 (P = 0.285) 1 0.803 (P < 0.001) 0.387 (P = 0.269) 0.354 (P = 0.126)
Eggshell thickness À0.275 (P = 0.240) À0.263 (P = 0.262) À0.147 (P = 0.537) 0.566 (P = 0.009) 1 0.548 (P = 0.101) 0.164 (P = 0.489)
T-Hg in eggshell À0.037 (P = 0.919) À0.042 (P = 0.909) À0.114 (P = 0.754) À0.795 (P = 0.006) À0.392 (P = 0.263) 1 0.134 (P = 0.712)
T-Hg in egg content À0.098 (P = 0.689) À0.120 (P = 0.625) À0.287 (P = 0.234) À0.140 (P = 0.568) À0.596 (P = 0.007) 0.143 (P = 0.714) 1
J. Olivero-Verbel et al. / Marine Pollution Bulletin 69 (2013) 105–109 107
4. (R = À0.596, P = 0.007). In both locations, a significant association
was observed between eggshell weight and eggshell thickness
(Cartagena Bay, R = 0.803, P < 0.001; Totumo Marsh, R = 0.566,
P = 0.009).
4. Discussion
E. Thula is a high-trophic level bird susceptible to pollution
exposure derived from its environment. In this study, several dif-
ferences in both egg morphometric and chemical parameters were
found between specimens collected at two places with different
history of Hg contamination at the north of Colombia. As expected,
eggshells from egrets living at Cartagena Bay had greater T-Hg con-
centrations than those from Totumo Marsh. This distribution of Hg,
based on proximity to anthropogenic sources, has been extensively
described for other bird species (Day et al., 2012; Hothem et al.,
2008; Anthony et al., 2007).
The mean difference in eggshell thickness registered in eggs
from Totumo Marsh in comparison with those from Cartagena
Bay reached 7 lm (3.6%). A reduction in eggshell thickness has
been associated with several factors, including exposure to high
concentrations of organochlorinated pesticides, such as dic-
hlorodiphenyldichloroethylene (DDE), polychlorinated biphenyls,
brominated flame retardants (Miljeteig et al., 2012; Gómez-Ramí-
rez et al., 2012; Malik et al., 2011; Fernie et al., 2009; Bouwman
et al., 2008), metals, such as magnesium, copper, zinc, lead and
Hg (Rodriguez-Navarro et al., 2002). Moreover, it has been widely
recognized that Hg reduces eggshell thickness and calcium content
of the egg (Lundholm, 1987). However, such negative associations
have also been absent in other studies (Miljeteig et al., 2012). De-
spite these contradictory findings, there is a general consensus that
Hg is a metal with a concern for breeding bird populations (Har-
greaves et al., 2011), and it has been suggested that thinning above
16–20% is associated with a decline in bird populations (Miljeteig
et al., 2012). Although such reductions in eggshell thickness were
not found in this study, additional studies should be conducted
to monitor this problem.
T-Hg concentrations found in egret eggshells from Cartagena
Bay (19.86 ± 1.88 ng/g) are similar to those reported for another
egret species, Egretta alba (21.02 ± 3.17 ng/g), from Northeast Chi-
na (Wang et al., 2012), and do not surpass the levels that may pro-
duce harmful effects on reproduction (Henny et al., 2002). The
levels of Hg in the eggs, which can be greater in egg content than
in eggshells (Aliakbari et al., 2011), as also observed here, may rep-
resent the level of the pollutant deposited by the females during
the egg formation period (Burger and Gochfeld, 1996), and that
could also have been derived from recent exposure or mobilization
from other tissues in the females (Burger et al., 1999).
It has been reported that lower egg weights may be associated
with Hg levels between 0.5 and 6 ppm (Burger and Gochfeld,
1997). However, although the concentrations reported here were
much lower than those affecting egg development, the differences
between sampling sites imply some degree of affectation in these
organisms. The differences may be the result of several factors.
First, the environmental conditions in both sites are quite unsimi-
lar. The bay is recognized as a source of Hg pollution, as more than
30 years ago, an extinct chlor-alkali plant released elemental Hg
into the bay as part of its operations. This contamination has been
documented for several environmental matrices, including sedi-
ments, fish, crabs and humans (Olivero-Verbel et al., 2009, 2008;
Carvajal et al., 2000). Second, other chemicals may also be in-
volved, as the bay is surrounded by several types of industries,
and chemicals such as perfluorooctyl sulphonates and polycyclic
aromatic hydrocarbons have been found in fish and sediments,
respectively (Johnson-Restrepo et al., 2008; Olivero et al., 2005b).
Third, it is possible that those egrets living in Cartagena may un-
dergo stress derived from human activities. Ecosystem pollution
by industries, transport, sewage disposal and deforestation linked
to urbanization may be pushing E. thula to increase their reproduc-
tion rate, probably by laying more eggs per clutch or season, but
with less time for the development of an optimal eggshell
thickness.
Results from Pearson correlation revealed different relation-
ships when data were analyzed for each sampling site. In the
bay, associations between morphometric variables seem more con-
sistent than in the reference site. This may be, in part, a conse-
quence of greater calcium content in eggshells from the polluted
site, as it has been shown that the structure of the shell is related
to crystal size and orientation (Dunn et al., 2012), and calcium lev-
els could be critical for adequate crystal formation. Greater calcium
concentrations in eggshells from the bay, in comparison with the
marsh, may derive from the food source for the egrets. On the
bay, these birds rely mostly on sea food, whereas in the marsh,
available food is impacted by freshwater inputs that reduce water
salinity, and therefore, calcium content.
In Cartagena Bay, T-Hg in egg content seems to be a function of
egg width (R = 0.455, P = 0.044), suggesting a possible bioaccumu-
lation process. Interestingly, although in this place, there is no
association between T-Hg in eggshell and eggshell weight
(R = 0.387, P = 0.269), in the marsh, eggshell weight decreased with
an increase in T-Hg (R = À0.795, P = 0.006). Moreover, at this less
polluted site, T-Hg in egg content also negatively correlated with
eggshell thickness (R = À0.596, P = 0.007), results that probably im-
ply that in the marsh, despite T-Hg levels are smaller than in the
bay, egrets are more susceptible to Hg exposure, probably as a con-
sequence of less calcium availability; whereas birds from the bay,
may have reached some tolerance to this pollutant.
As eggshell thickness strongly influences bird survival (Castilla
et al., 2010), actions should be taken to decrease Hg levels in the
bay, including recovering mangrove areas for egrets and other
birds to nest, deforestation prevention and avoiding ecosystem
damage by littering or pollution.
5. Conclusion
The results found on this study showed, for the first time, the
impact of environmental contamination on E. thula living at Carta-
gena Bay. T-Hg and calcium concentrations were greater than
those found in Totumo Marsh, the reference site, and this pollutant
may be one of the factors responsible for the smaller eggshell
thickness observed in eggs from E. thula in the bay.
Acknowledgements
This work was supported by COLCIENCIAS (Bogota, Colombia)
and the University of Cartagena (Cartagena, Colombia), Grant No.
1107-04-16346. The authors also thank fishermen from Cartagena
Bay and Totumo Marsh, Jorge Ropero, William Ortiz and Alveiro
Hernández for their collaboration. Special thanks to Propilco,
Cartagena for helping with calcium content analysis.
References
Ackerman, J.T., Overton, C.T., Casazza, M.L., Takekawa, J.Y., Eagles-Smith, C.A.,
Keister, R.A., Herzog, M.P., 2012. Does mercury contamination reduce body
condition of endangered California clapper rails? Environ. Pollut. 162, 439–448.
Aliakbari, A., Savabieasfahani, M., Ghasempouri, S.M., 2011. Mercury in egg and
eggshell of Whiskered Tern (Chlidonias hybrida) from Anzali wetlands of the
Caspian Sea, Iran. Bull. Environ. Contam. Toxicol. 86, 175–179.
Anthony, R.G., Miles, A.K., Ricca, M.A., Estes, J.A., 2007. Environmental contaminants
in bald eagle eggs from the Aleutian archipelago. Environ. Toxicol. Chem. 26,
1843–1855.
108 J. Olivero-Verbel et al. / Marine Pollution Bulletin 69 (2013) 105–109
5. Bouwman, H., Polder, A., Venter, B., Skaare, J.U., 2008. Organochlorine contaminants
in cormorant, darter, egret, and ibis eggs from South Africa. Chemosphere 71,
227–241.
Bryan Jr., A.L., Brant, H.A., Jagoe, C.H., Romanek, C.S., Brisbin Jr., I.L., 2012. Mercury
concentrations in nestling wading birds relative to diet in the southeastern
United States: a stable isotope analysis. Arch. Environ. Contam. Toxicol. 63,
144–152.
Burger, J., Gochfeld, M., 1996. Heavy metal and selenium levels in Franklin’s gull
(Larus pipixcan) parents and their eggs. Bull. Environ. Contam. Toxicol. 30, 487–
491.
Burger, J., Gochfeld, M., 1997. Risk, mercury levels, and birds: relating
adverse laboratory effects to field biomonitoring. Environ. Res. 75, 160–
172.
Burger, J., Woolfenden, G., Gochfeld, M., 1999. Metal concentrations in the eggs of
endangered Florida scrub-jays from central Florida. Bull. Environ. Contam.
Toxicol. 37, 385–388.
Carvajal, D., Pineda, P., Olivero, J., Campos, N., González, H., 2000. Mercury
levels in muscle of two fish species and sediments from the Cartagena Bay
and the Ciénaga Grande de Santa Marta, Colombia. Environ. Pollut. 109,
157–163.
Castilla, A.M., Herrel, A., Robles, H., Malone, J., Negro, J.J., 2010. The effect of
developmental stage on eggshell thickness variation in endangered falcons.
Zoology (Jena) 113, 184–188.
Costa, M.F., Landing, W.M., Kehrig, H.A., Barletta, M., Holmes, C.D., Barrocas, P.R.,
Evers, D.C., Buck, D.G., Claudia Vasconcellos, A., Hacon, S.S., Moreira, J.C., Malm,
O., 2012. Mercury in tropical and subtropical coastal environments. Environ.
Res. 119, 88–100.
Day, R.D., Roseneau, D.G., Vander Pol, S.S., Hobson, K.A., Donard, O.F., Pugh, R.S.,
Moors, A.J., Becker, P.R., 2012. Regional, temporal, and species patterns of
mercury in Alaskan seabird eggs: mercury sources and cycling or food web
effects? Environ. Pollut. 166, 226–232.
Dolbec, J., Mergler, D., Larribe, F., Roulet, M., Lebel, J., Lucotte, M., 2001. Sequential
analysis of hair mercury levels in relation to fish diet of an Amazonian
population, Brazil. Sci. Total Environ. 271, 87–97.
Dunn, I.C., Rodríguez-Navarro, A.B., Mcdade, K., Schmutz, M., Preisinger, R.,
Waddington, D., Wilson, P.W., Bain, M.M., 2012. Genetic variation in eggshell
crystal size and orientation is large and these traits are correlated with shell
thickness and are associated with eggshell matrix protein markers. Anim.
Genet. 43, 410–418.
Fernie, K.J., Shutt, J.L., Letcher, R.J., Ritchie, I.J., Bird, D.M., 2009. Environmentally
relevant concentrations of DE-71 and HBCD alter eggshell thickness and
reproductive success of American kestrels. Environ. Sci. Technol. 43, 2124–
2130.
Gómez-Ramírez, P., Martínez-López, E., García-Fernández, A.J., Zweers, A.J., van den
Brink, N.W., 2012. Organohalogen exposure in a Eurasian Eagle owl (Bubo bubo)
population from Southeastern Spain: temporal–spatial trends and risk
assessment. Chemosphere 88, 903–911.
Haines, T., May, T., Finlayson, R., Mierzykowski, S., 2003. Factors affecting food chain
transfer of mercury in the vicinity of the Nyanza Site, Sudbury River,
Massachusetts. Environ. Monit. Assess. 86, 211–232.
Hargreaves, A.L., Whiteside, D.P., Gilchrist, G., 2011. Concentrations of 17 elements,
including. Sci. Total Environ. 409, 3757–3770.
Helander, B., Olsson, A., Bignert, A., Asplund, L., Litzén, K., 2002. The role of DDE,
PCB, Coplanar PCB and eggshell parameters for reproduction in the White-tailed
Sea Eagle (Haliaeetus albicilla) in Sweden. Ambio 31, 386–403.
Henny, C., Hill, E., Hoffman, D., Spalding, M., Grove, R., 2002. Nineteenth century
mercury: hazard to wading birds and cormorants of the Carson River, Nevada.
Ecotoxicology 11, 213–231.
Hothem, R.L., Trejo, B.S., Bauer, M.L., Crayon, J.J., 2008. Cliff swallows Petrochelidon
pyrrhonota as bioindicators of environmental mercury, Cache Creek Watershed,
California. Arch. Environ. Contam. Toxicol. 55, 111–121.
Johnson-Restrepo, B., Olivero-Verbel, J., Lu, S., Guette-Fernández, J., Baldiris-
Avila, R., O’Byrne-Hoyos, I., Aldous, K.M., Addink, R., Kannan, K., 2008.
Polycyclic aromatic hydrocarbons and their hydroxylated metabolites in
fish bile and sediments from coastal waters of Colombia. Environ. Pollut.
151, 452–459.
Kannan, K., Corsolini, S., Falandysz, J., Fillmann, G., Shenthil-Kumar, K., Loganathan,
B., Ali-Mohd, M., Olivero, J., Van Wouwe, N., Ho Yang, J., Aldous, K., 2004.
Perfluorooctanesulfonate and related fluorochemicals in human blood from
several countries. Environ. Sci. Technol. 38, 4489–4495.
Leoni, L., Saitta, M., 1976. X-ray fluorescence analysis of 29 trace elements in rock
and mineral standards. Rend. Soc. It. Min. Pet. 32, 497–510.
Lundholm, C.E., 1987. Methyl mercury decreases the eggshell thickness and
inhibits the Ca2+
uptake in a homogenate of the eggshell gland mucosa and
its subcellular fractions from the domestic fowl. Pharmacol. Toxicol. 60, 385–
388.
Malik, R.N., Rauf, S., Mohammad, A., Eqani, S.A., Ahad, K., 2011. Organochlorine
residual concentrations in cattle egret from the Punjab Province, Pakistan.
Environ. Monit. Assess. 173, 325–341.
Miljeteig, C., Gabrielsen, G.W., Strøm, H., Gavrilo, M.V., Lie, E., Jenssen, B.M., 2012.
Eggshell thinning and decreased concentrations of vitamin E are associated
with contaminants in eggs of ivory gulls. Sci. Total Environ. 431, 92–99.
Olivero, J., Baldiris, R., Arroyo, B., 2005a. Nematode infection in Mugil incilis (lisa)
from Cartagena Bay and Totumo Marsh, north of Colombia. J. Parasitol. 91,
1109–1112.
Olivero, J., Guette, J., Baldiris, R., O´ Byrne, I., Tao, L., Johnson, B., Kannan, K., 2005b.
Perfluorooctane sulfonate and related fluorochemicals in biological samples
from the north coast of Colombia. Environ. Pollut. 142, 367–372.
Olivero-Verbel, J., Caballero-Gallardo, K., Torres-Fuentes, N., 2009. Assessment of
mercury in muscle of fish from Cartagena Bay, a tropical estuary at the north of
Colombia. Int. J. Environ. Health Res. 19, 343–355.
Olivero-Verbel, J., Johnson-Restrepo, B., Baldiris-Avila, R., Güette-Fernández, J.,
Magallanes-Carreazo, E., Vanegas-Ramírez, L., Kunihiko, N., 2008. Human
and crab exposure to mercury in the Caribbean coastal shoreline of
Colombia: impact from an abandoned chlor-alkali plant. Environ. Int. 34,
476–482.
Rodriguez-Navarro, A.B., Gaines, K.F., Romanek, C.S., Masson, G.R., 2002.
Mineralization of clapper rail eggshell from a contaminated salt Marsh
system. Arch. Environ. Contam. Toxicol. 43, 449–460.
Sholupov, S., Pogarev, S., Ryzhov, V., Mashyanov, N., Stroganov, A., 2004. Zeeman
atomic absorption spectrometer RA-915+ for direct determination of mercury
in air and complex matrix samples. Fuel Process. Technol. 85, 473–485.
Sugni, M., Tremolada, P., Porte, C., Barbaglio, A., Bonasoro, F., Carnevali, M.D., 2010.
Chemical fate and biological effects of several endocrine disrupters compounds
in two echinoderm species. Ecotoxicology 19, 538–554.
Tamponi, M., Bertoli, M., Innocenti, F., Leoni, L., 2003. X-ray fluorescence analysis of
major elements in silicate rocks using fused glass discs. Atti Soc. Tosc. Sci. Nat.
Mem. Ser. A 108, 73–79.
Walpole, R., Myers, R., Myers, S., Ye, K., 2001. Probability and Statistics for Engineers
and Scientists, seventh ed. Prentice Hall, London.
Wang, Q., Zhang, Z., Zhou, X., Lu, X., 2012. Mercury distribution and accumulation in
typical wetland ecosystems of Sanjiang Plain, Northeast China. Chin. Geogr. Sci.
22, 1–10.
Xu, L.Q., Liu, X.D., Sun, L.G., Chen, Q.Q., Yan, H., Liu, Y., Luo, Y.H., Huang, J., 2011. A
700-year record of mercury in avian eggshells of Guangjin Island, South China
Sea. Environ. Pollut. 159, 889–896.
J. Olivero-Verbel et al. / Marine Pollution Bulletin 69 (2013) 105–109 109