Poster presentaed at the XIVth European Poultry Conference, 23-27 June 2014, Norway. Clasadonte et al.

1. Stability of red and yellow carotenoids
in stored poultry premix
DSM Nutritional Products
L. Clasadonte, M. Gadient, J. Schierle, C. Hamelin
91%
79% 79%
52% 51%
35%
CXN-1 CXN-2 CIT CXN-3 CXN-4 CAPS
Recoveryin%ofinitialcontent
Products
a b b c c d
Conclusion:
Stability of carotenoids is difficult to achieve. The value of a quality
protection can be measured in each step of feed manufacturing and
summed up as total stability. Higher stability means more product fed
to the animals and more product to be deposited in egg yolk or skin.
When comparing the total stability of CAROPHYLL Red 10% with
other carotenoids collected in the market results show leading position
of this canthaxanthin product.
Product Origin Storage
As such
stability
Premix
stability
Feed
stability
Total
Stability
Total
Stability
(% CXN-1)
CXN-1 Europe 3 months 94% 91% 77% 66% 100%
CXN-2 Europe 3 months 91% 79% 53% 38% 58%
CIT Europe 3 months 94% 79% 57% 43% 65%
CXN-3 Asia 3 months 69% 52% 51% 18% 27%
CXN-4 Europe 3 months 92% 51% 69% 32% 49%
CAPS Asia 3 months 60% 35% 61% 13% 20%
Introduction:
Carotenoids are oxygen, heat, pH and light sensitive. In order to make
these pure, crystalline and lipophilic compounds suitable for industrial
application protection is required. This is realized via formulation,
which is known to considerably influence the stability performances of
feed additives. Formulation strategies result in very different products
as shown in Figure 1.
Methodology:
- Products:
- Red carotenoids: CAROPHYLL Red 10% (CXN-1), other
canthaxanthin (CXN-2, CXN-3, CXN-4), citranaxanthin (CIT),
capsanthin/capsorubin (CAPS)
- Yellow carotenoids: CAROPHYLL Yellow 10% (APO-1), other
apoester (APO-2, APO-3), lutein/zeaxanthin (LUT)
- Complete premix: 9% trace minerals, 30% choline chloride
- Storage: 3 months at 30°C and 65% relative humidity
- Carotenoid assay: HPLC method
- Statistics: multiple comparison of means following the Tukey
method. Same letter means that results do not differ
significantly (P<0.05)
Results:
91%
80%
39%
20%
APO-1 APO-2 APO-3 LUT
Recoveryin%ofinitialcontent
Products
a b c d
Fig. 2. Premix stability of red carotenoids after 3 months storage
Further Information
L. Clasadonte, DSM Nutritional Products, Nutrition R&D Center Forms and Application,
laure.clasadonte@dsm.com
Fig. 1. Light microscopy pictures of carotenoid products
CXN-1 (CAROPHYLL Red 10%) CXN-2 CXN-3
CXN-4CIT CAPS
Fig. 3. Premix stability of yellow carotenoids after 3 months storageAPO-1 (CAROPHYLL Yellow 10%)
APO-2 APO-3