The document discusses a conference on microbiology of the gut held in Delft, Netherlands. It covers topics including the importance of intestinal flora, the stages of bacterial colonization in the gut from birth through senior years, and the most common resident and transient bacteria. It also discusses how prebiotics, probiotics, and diet can influence gut flora, noting the acid-alkaline balance and importance of fiber and fermented foods. The presentation encourages adopting a new balance through these foods and colon hydrotherapy.

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United States / Europe
Microbiology of the Gut
I-ACT convention June 23, 2012
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Europe The Netherlands
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The Netherlands, Delft
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Topics
• Important information of the intestinal flora
• What does the flora do and where
• Healthy food for your gut flora
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Importance of the intestinal flora
• Colon environment
• Barrier against micro organisms
• Stimulating metabolism and blood flow in the
intestinal mucosa
• Stimulating the peristalsis
• Availability of vitamins
• Influence on the immune system
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Stages of the bacterial colonisation
The bacterial development
PHASE 1 PHASE 2 PHASE 3 PHASE 4
• Birth itself is already decisive for the 1st-2nd weeks Commencing Commencing Senior age
development of immunity of life from 3rd week from the start of
of life weaning and up
to senior ages
• The colonisation by the micro-organisms is Take up of Predominantly Various types of Clostridien é
mutually coordinated environmental micro-aerophile strictly anaerobic Lactobacilli é
germs at birth and anaerobic micro Bifido bacteria
acidic flora organismsé
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Bacterial composition of the gut flora
Esophagus
Food bacteria Feces flora
Duodenum 101- 104/ml
Stomach 101- 103/ml
Lactobacilli 101 - 103
Lactobacilli 101 - 103
Enterococci sp. 101 - 103
Enterobacteria 0 - 102
Enterococci sp. 101 - 103
Enterobacteria 0 - 102
• Resident flora
Bacteroides sp. 0 - 10 2
Bacteroides sp. 0 - 102 Is wall attached, native flora
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– Aerobic flora < 1%
Colon 10 - 10 /g faeces
Bifidobacteria 109 – 1011 – Anaerobic flora >99 %
Bacteroides sp. 109 - 1011
Ileum
105- 108/g faeces Enterobacteria 105 – 107 • Transient flora
Enterococci 104 - 105
Bacteroides sp. 104 - 10 7
Lactobacilli 104 - 105 Are unable to remain in the body for extended periods
Lactobacilli 104 - 106
Clostridien sp. 102 – 105
Enterococci sp. 104 - 106
Fusobacteria 103 - 105
– Aerobic flora < 1%
Enterobacteria 104 - 106
Veillonella sp. 103 – Anaerobic flora >99 %
Stafylococci 103
Yeasts 103 • Yeast and parasites
Enterobacteriacae < 104
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Resident intestinal bacteria Transient intestinal bacteria
• Aerobes • Aerobes
– E. coli, Enterococci – Klebsiella sp.
• Micro-aerophiles – Enterobacteria
• Proteus sp, Morganella sp, Citrobacter sp
– Lactobacilli
– Pseudomonas sp.
• Anaerobes
• Anaerobes
– Bifido bacteria
– Clostridia
– Bacteroides spp.
• Bacteroids-Prevotella-Porphyromonas group
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The most important yeasts and fungi
• Yeasts • Fungi
– Candida albicans – Aspergillus fumigatus
– var. stellatoidea – Aspergillus niger
– Candida glabrata – Aspergillus flavus
– Candida guillermondii – Alternaria alternata
– Candida krusei – Mucor species
– Candida parapsilosis – Rhizomucor pusillus
– Candida tropicalis – Cunninghamella elegans
– Trichosporon cutaneum
– Cryptococcus neoformans
– Geotrichum candidum
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How can you influence the intestinal
Prebiotics
flora?
• Prebiotics • Non-digestible food
ingredients that
beneficially affect the host
by selectively stimulating
• Metabiotics the growth and/or activity
of one or a limited number
of bacteria in the colon,
and thus improve host
health.
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Which fibers stimulates which the
Prebiotics
bacteria in the gut
• Fibers • Cellulose – grow of bifidobacteria
– 30 – 40 gram per day
• Soluble fibers are: • Lignins – against parasites
– Beta-glucans, gums, mucilages and pectins.
– Oat products, legumes, apples, bananas, berries, broccoli carrots,
• Arabinogalactans – against transient flora
root vegetables (potatoes and yams – the skin has insoluble fiber)
• Inulin - grow of lactobacilli
• Insoluble fibers are:
– Cellulose and lignin • Beta-glucan – stimulates the immune system
– Whole grains; bran; nuts and seeds; vegetables such as green
beans, cauliflower, zucchini, celery, and skins of some fruits and root
vegetables.
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Soluble fibers Insoluble fibers
• Arabinogalactans are a type of soluble fiber made up of • Cellulose is a substance found in plant cells, surrounding
long, branching chains of sugars. and inside the cell wall. It is an insoluble fiber
– Carrots, radish, black beans, pear, tomato, shiitake and coconut – High in cellulose are cruciferous: Broccoli, Brussels Sprout, Cabbage,
Collard Greens, Kale, Horseradish, Rutabaga, Turnip, Chinese
• Inulin is a carbohydrate belonging to a class of compounds
Cabbage, Cauliflower, Broccoli Rabe, Daikon, Bok Choy, Radish,
known as fructans and is closely related to fructo- Kohlrabi
oligosaccharides (FOS). • Lignins are an integral part of the secondary cell walls of
– Leeks, artichokes, asparagus, onions, garlic, bananas, wheat, rye,
and chicory root
plants and some algae
– Lignins are found in all vegetables. There is more lignin in the plant
• Beta-glucan is a complex carbohydrate made from when harvested at later growth stages.
individual glucose molecules
– Oat, barley, rye, wheat, mushrooms and algae
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Metabiotics Acid – alkaline balance
Metabiotics are the metabolic by-products of • The benefits
probiotics
• The most important of these metabiotics are short-chain
fatty acids • Influence on the gut flora
– Fermented foods such as pickles, saurkraut, kimchi, sourdough
bread, apple cider and miso
– Yoghurt if…!
– Aged cheeses (aged more then 6 months) • Influence in the body
– Kefir and kombucha
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A new balance for your clients Don’t forget
• Prebiotic and metabiotic food • Two years after birth the gut flora is established
– More fibers and fermented food
• It is hard to change the microbiology of the gut
• Acid – alkaline balance
• Encourage your clients to eat healthy
• Colonhydrotherapy
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Thank you
Lisette Timmermans
Delft, The Netherlands
www.lisette–timmermans.nl (in dutch)
www.colonhydrotherapie.nl (in dutch)
info@lisette-timmermans.nl
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