SlideShare a Scribd company logo
• Introduction 
• Note 
• Importance 
• Features 
• Working 
• Conclusion
• With over 60,000 downloads to date, Geospiza’s FinchTV 
is the popular way to view DNA sequence traces on 
Linux, Mac OSX, Windows, and Solaris. FinchTV started 
as the only chromatogram viewer that can display an 
entire trace in a scalable multi-pane view. And it leads the 
way with raw data views, BLAST searching and the 
ability to reverse complement sequences and traces.
• FinchTV allows the user to see their chromatogram 
along with the nucleotide base assigned to each peak but 
does not give a confidence value for each base. Phred 
allows the user to do high throughput analysis but in 
doing so doesn't allow the user to see each 
chromatogram. Phrap assembles multiple reads of the 
same sequence into a single sequence.
1. FinchTV is not a base caller. It can only show base calls 
that are either added by a DNA sequencing instrument or by 
base calling programs like phred (scf files). 
2. FinchTV can show quality values. If a labs has an ABI 3730 
and uses KB base caller, then the .ab1 files will contain quality 
values and these will be displayed in FinchTV. 
Alternatively, if a lab uses phred for base calling and makes 
scf files with quality values, FinchTV will display those quality 
values. 
I have some blog posts here that show screen shots of how 
this works and how phred compares with the KB base caller 
at:
• The program has the following features: 
• Reads chromatogram files in the most popular chromat formats. 
• Displays quality values above the trace (when available). 
• Displays the base sequence in multi-colour above the trace along with 
their positions in the sequence. 
• Allows adjustment of vertical and horizontal scales. 
• Allows display of the chromatogram trace as a single pane or in a 
wrapped view. 
• Allows you to view the DNA sequence in the Chromatogram Info dialog 
and additional chromatogram data details when available. 
• Provides you with the ability to print out your trace all on one page or 
with a set number of panels per page. 
• Gives the option of exporting the DNA sequence to a FASTA formatted 
text file.
• How does this work? 
1. Log in to your Finch account. Open a chromatogram in 
FinchTV either by clicking the FinchTV icon or the link 
from the Chromat Read page that says Open in FinchTV 
2. When you're ready to enter information, click the 
Commit button (outlined below).
• FinchTV is an important software and students use this 
software to enter their blast results in to iFinch. This has 
been preety helpful. The data get stored for each 
chromatogram and we can all view the results.

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Botany software

  • 1.
  • 2. • Introduction • Note • Importance • Features • Working • Conclusion
  • 3. • With over 60,000 downloads to date, Geospiza’s FinchTV is the popular way to view DNA sequence traces on Linux, Mac OSX, Windows, and Solaris. FinchTV started as the only chromatogram viewer that can display an entire trace in a scalable multi-pane view. And it leads the way with raw data views, BLAST searching and the ability to reverse complement sequences and traces.
  • 4.
  • 5. • FinchTV allows the user to see their chromatogram along with the nucleotide base assigned to each peak but does not give a confidence value for each base. Phred allows the user to do high throughput analysis but in doing so doesn't allow the user to see each chromatogram. Phrap assembles multiple reads of the same sequence into a single sequence.
  • 6. 1. FinchTV is not a base caller. It can only show base calls that are either added by a DNA sequencing instrument or by base calling programs like phred (scf files). 2. FinchTV can show quality values. If a labs has an ABI 3730 and uses KB base caller, then the .ab1 files will contain quality values and these will be displayed in FinchTV. Alternatively, if a lab uses phred for base calling and makes scf files with quality values, FinchTV will display those quality values. I have some blog posts here that show screen shots of how this works and how phred compares with the KB base caller at:
  • 7. • The program has the following features: • Reads chromatogram files in the most popular chromat formats. • Displays quality values above the trace (when available). • Displays the base sequence in multi-colour above the trace along with their positions in the sequence. • Allows adjustment of vertical and horizontal scales. • Allows display of the chromatogram trace as a single pane or in a wrapped view. • Allows you to view the DNA sequence in the Chromatogram Info dialog and additional chromatogram data details when available. • Provides you with the ability to print out your trace all on one page or with a set number of panels per page. • Gives the option of exporting the DNA sequence to a FASTA formatted text file.
  • 8. • How does this work? 1. Log in to your Finch account. Open a chromatogram in FinchTV either by clicking the FinchTV icon or the link from the Chromat Read page that says Open in FinchTV 2. When you're ready to enter information, click the Commit button (outlined below).
  • 9.
  • 10. • FinchTV is an important software and students use this software to enter their blast results in to iFinch. This has been preety helpful. The data get stored for each chromatogram and we can all view the results.