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CELL FRACTIONATION

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CELL FRACTIONATION AND ITS STEPS

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CELL FRACTIONATION

  1. 1. LE 6-5A Homogenization Homogenate Tissue cells Differential centrifugation
  2. 2. • Tissue is cut into pieces and placed in cold isotonic buffer solution. • Pieces of tissue are ground into very small fragments in a type of liquidiser called as homogeniser. • The suspension of cell fragments is filtered to remove large pieces of tissue debris • The filterate is then placed in a centrifuge

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