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Dna replication slide

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Dna replication slide

  1. 1. *
  2. 2. 1. Take out PD and Reading Annotations2. Take 1 sheet of DNA – Double Helix Game, DNA replication Notes, and 1 laptop per table. Log-on and go to nobelprize.org. Then click on the educational tab on top of the menu3. Swap and Comment on PDs *
  3. 3. Objective: be the fastest team that makes a copyof DNA with the least amount of nucleotides sothat you end up with 2 copies of DNA in the endRules:- No talking during the activity- 2 min to discuss strategy before activity- When finished, tape your 2 DNA strands on the white board as shown- The game does not end until the top 3 teams are established http://www.online-stopwatch.com/
  4. 4. Fun Fact:Speed of DNA replication in human cells50bps/secSpeed of DNA replication in bacterial cells1000bps/sec *
  5. 5. 1. What are some differences and similarities between DNA and RNA2. Draw how the two strands of DNA align. Label the 5’ (prime) and 3’ (prime) ends.(draw out the molecules) What is this type of alignment called?3. What is going well in this class so far? What are your criticisms of this class? *
  6. 6. http://www.youtube.com/watch?v=bee6PWUgPo8&feature=relmfu
  7. 7. DNA Replication means to Make copies of DNADNA Replication happens when new cells need to be made Mitosis and Meiosis
  8. 8. DNA Replication Parental strand Daughter stand
  9. 9. DNA goes through a type of replication called semi-conservative replication, which means one strand onthe DNA helix is new and the other strand is old
  10. 10. How does DNA replicate? Step 1: DNA Helix unwinds and the 2 strands separate.
  11. 11. How does DNA replicate? Step 2: Add free nucleotides that hydrogen bond to the original strand
  12. 12. How does DNA replicate? Step 3: Phosphates bond with sugar to form the backbone of the new nucleotides
  13. 13. How DNA replicates in prokaryotes Have one origin of replication
  14. 14. How DNA replicates in eukaryotes Have multiple origins of replication
  15. 15. The main difference between replication in prokaryotes andeukaryotes is that prokaryotes have one origin of replication andeukaryotes have many
  16. 16. Mechanism of DNA Replication:http://www.youtube.com/watch?v=teV62zrm2P0
  17. 17. Replication Bubble: (look in DNA packet)
  18. 18. DNA REPLication Real Time Advanced:http://www.youtube.com/watch?v=I9ArIJWYZHI
  19. 19. Replication fork: • Where the DNA strands split off • Moves with the path of the replication bubblePrimer: Short sequence of RNA that binds to the lagging parental strand in order for DNA polymerase to copy the lagging strnadPrimase: A protein that binds to the parental lagging strand to make a primerHelicase: A protein that unwinds DNA
  20. 20. Single strand binding protein: A protein that binds each DNA strand to stabilize it and hold it apartDNA polymerase: A protein that adds free nucleotides from 5’ to 3’ to make a new strand of DNA. Also fixes mistakes in DNADNA ligase: A protein that binds the phosphates of nucleic acids to the sugar group of adjacent nucleic acids
  21. 21. A. Using Conservative replicationB. Using Dispersive replicationC.Using Liberal replicationD.Using Semi-conservative replication
  22. 22. A. DNA PolymeraseB. HelicaseC.PrimaseD.LigaseE. Unwindase
  23. 23. A. Leading StrandB. Following StrandC. Lagging StrandD. Continuous Strand
  24. 24. A. Eukaryotes have a nucleusB. Prokaryotes use conservative replication and Eukaryotes use semi-conservative.C. Eukaryotes have multiple origins of replication and prokaryotes only have oneD. There is no difference
  25. 25. *
  26. 26. PCR HIDDEN HISTORY! *
  27. 27. * http://www.hulu.com/watch/101375/the- nobel-prize-he-surfs-he-walks-mazes-he- invents-pcr-profile-of-biochemist-kary-mullis
  28. 28. * PCR stands for Polymerase Chain Reaction* PCR is a scientific technique to amplify (make large copies) of a specific sequence of DNA
  29. 29. * What protein unwinds DNA* What protein adds nucleotides to make the new strand of DNA* Draw a general diagram of how DNA replication works or Explain how DNA replication works* Happy Friday! *
  30. 30. 1. DNA Template2. dNTPs (deoxyribonucleotide)3. Taq Polymerase4. Primers5. Buffer and MgCl *
  31. 31. * http://www.invitrogen.com/site/us/en/home/ Products-and-Services/Applications/PCR/pcr- animation.html *
  32. 32. * http://www.invitro gen.com/site/us/e n/home/Products- and- Services/Applicatio ns/PCR/pcr- animation.html*
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