Make an experiment with this toolkit listed. Experiment about digestive enzymes in animals by showing the steps and results. The Toolkit available samples of enzymes (assume that all enzymes are at appropriate concentration in liquid buffer) The Toolkit available enzymes trypsin trypsinogen chymotrypsin chymotrypsinogen enteropeptidase prophospholipase A2 phospholipase A2 PSTI Note: Available pancreatic enzymes, all at appropriate concentration in liquid buffer. available non-enzyme substrates whole milk (source of lipids), in liquid form gelatin (source of collagen protein), bound to developed film Note:Available non-enzyme substrates: whole milk (left), gelatin and developed film strips (right). Use Available detection techniques Phenol red pH indicator technique for detecting lipid digestion rate: A colour-changing pH detector, phenol red, is added to the test tube with a source of lipids and a lipase. As the lipase digests lipids, breaking the lipids down into fatty acids, the solution gradually becomes more acidic and the indicator gradually turns from pink to yellow. The faster the digestion happens, the faster the colour change happens. (Bishop et al. 2012) Fill in table: Summary of experimental design to test a hypothesis about the role of PSTI in pancreatic enzyme function. Hypothesis PSTI inhibits trypsin directly. Treatment Condition Assay Control Condition Results of Control Condition Assay Predicted Results of Treatment Condition Assay if Hypothesis is Supported Predicted Results of Treatment Condition Assay if Hypothesis is NOT Supported The Toolkit available enzymes trypsin trypsinogen chymotrypsin chymotrypsinogen enteropeptidase prophospholipase A2 phospholipase A2 PSTI Note: Available pancreatic enzymes, all at appropriate concentration in liquid buffer..

1. Make an experiment with this toolkit listed.
Experiment about digestive enzymes in animals by showing the steps and results.
The Toolkit available samples of enzymes (assume that all enzymes are at appropriate
concentration in liquid buffer)
The Toolkit
available enzymes
trypsin
trypsinogen
chymotrypsin
chymotrypsinogen
enteropeptidase
prophospholipase A2
phospholipase A2
PSTI
Note: Available pancreatic enzymes, all at appropriate concentration in liquid buffer.
available non-enzyme substrates
whole milk (source of lipids), in liquid form
gelatin (source of collagen protein), bound to developed film
Note:Available non-enzyme substrates: whole milk (left), gelatin and developed film strips
(right).
Use Available detection techniques
Phenol red pH indicator technique for detecting lipid digestion rate: A colour-changing pH
detector, phenol red, is added to the test tube with a source of lipids and a lipase. As the lipase
digests lipids, breaking the lipids down into fatty acids, the solution gradually becomes more
acidic and the indicator gradually turns from pink to yellow. The faster the digestion happens,
the faster the colour change happens. (Bishop et al. 2012)
Fill in table:
Summary of experimental design to test a hypothesis about the role of PSTI in pancreatic
enzyme function.

2. Hypothesis
PSTI inhibits trypsin directly.
Treatment Condition
Assay
Control Condition
Results of Control Condition Assay
Predicted Results of Treatment Condition Assay if Hypothesis is Supported
Predicted Results of Treatment Condition Assay if Hypothesis is NOT Supported
The Toolkit
available enzymes
trypsin
trypsinogen
chymotrypsin

3. chymotrypsinogen
enteropeptidase
prophospholipase A2
phospholipase A2
PSTI
Note: Available pancreatic enzymes, all at appropriate concentration in liquid buffer.