1. The __ is the point in time to which lost syste.docx

1. The __________ ________is the point in time to which lost
systems and data can be recovered after an outage as determined
by the business unit.
2. The _____________________________ is the point in time to
which lost systems and data can be recovered after an outage as
determined by the business unit.
3. A(n) ________________is the collection of individuals
responsible for the overall planning and development of the
contingency planning process, including the organization of
subordinate teams and oversight of subordinate plans.
4. A(n)_____________is a commonly used technique for
collecting information directly from the end users and business
managers.
5. A(n)______________is the long-term storage of a document
or data file, usually for legal or regulatory purposes.

6. The most common method of calculating business impact is
to review financial reports and budgets.
True
False
7. The CP policy is the formal policy that will guide the efforts
of the subordinate teams in developing their plans, and the
overall operations of the organization during contingency
operations.
True
False
8._______________ is used to understand the ways systems
operate and to chart process flows and interdependency studies
for both manual and automated systems.
9._________________ is a task performed by an organization or
organizational subunit in support of the organization’s overall
mission.

10. Among the most critical start-up tasks of the CPMT is
aligning support.
True
False

2) OSMOSIS AND DIALYSIS
Click Mouse Here to View Next Text on the Blackboard
Click Here To Start Experiment #2
Sucrose

To magnify the blackboard click on it to view a discussion on
membrane transport
There are two parts to this experiment # 2. PART 1 will
consider OSMOSIS and PART 2 will consider DIALYSIS. You
will need to supply the following in your lab report for
experiment # 2
Part 1 (Osmosis)
Table of Osmotic Potential and Time

Graph of Osmotic Potential vs Time
Graph of Osmotic Change vs Time (Osmotic Rate)
Answers to Questions (1 - 7)
Part 2 (Dialysis)
Table of Dialysis Tests
Answers to Questions (1 - 6)

OSMOSIS is the diffusion of water through a selectively
permeable membrane. Water will generally move quite freely
through the cell membrane by diffusion. Osmotic movement of
water occurs when the solute (non-penetrating) concentrations
differ between the opposing sides of the cell membrane. A
difference in solute (non-penetrating) concentrations means
there is a difference in water concentrations and water will
move from the region of higher concentration to a region of
lower concentration.

For example, water osmotically moves into a cell when the fluid
outside the cell has more water (less solutes) than the fluid
inside the cell. In this case, as water moves into the cell, it

swells as the water pressure inside the cell increases.
Red Blood Cell
Distilled Water

DIALYSIS is the separation of solutes according to their size by
diffusion through a permeable membrane. Depending upon the
molecular pore size of the membrane, solutes will either diffuse
across the membrane or be restricted by their size.
The device you will be using to collect data for the experiments
is an osmometer. This is a device used to measure osmotic
force.

PART 1 (OSMOSIS)
MATERIALS
Thistle tube (with mm markings)
Thistle tube stand

Dialysis tubing (selectively permeable membrane)
Rubber band
Sugar solution (20 % sucrose)
Beaker

*
Click Mouse Here to View Next Instructions on the Blackboard
H2O
Sucrose
H2O

Sucrose
You will need to record your data from this experiment on your
Data Table of Osmotic Potential and Time.
*

Procedure
Click on the thistle tube stand in order to bring it to the table
You will know you are pointing to the stand when the cursor
changes.
H2O
Sucrose
H2O
Sucrose
STAND
*

Now click on the thistle tube funnel to attach it to the tube
stand.
H2O
Sucrose
H2O

Next we will put the dialysis tubing on the bulb end of the
thistle tube. You will need only a small piece. Click on the
dialysis tubing on the shelf.
H2O
Sucrose
H2O
Sucrose
Tubing

Put a rubber band on the tube and dialysis tubing to hold it in
place. You can accomplish this by clicking on the rubber bands
on the shelf.
H2O
Sucrose
H2O
Sucrose
Rubber Band

*
Put the bulb of the thistle tube into the beaker of water. The
water is distilled which means that it has no solutes in it (100 %
water). Click on the beaker labeled as H2O.

H2O
Sucrose
Sucrose
H2O

*
Now we will pour the sucrose solution into the thistle tube from
the top. The sucrose solution is a 20 % sugar solution (80 %
water). Click on the beaker labeled as Sucrose.
Sucrose

Record the original millimeter measurement of the sucrose
solution in the column of the thistle tube on your data sheet.
Next, record the movement of the sucrose solution every 15
minutes for 90 minutes. Click on the start button of the clock to
begin measuring.

START
00:00
05:00
10:00
15:00
20:00
25:00
30:00
35:00
40:00
45:00
50:00
55:00
60:00
65:00
70:00
75:00
80:00
85:00
90:00
3
10
16
22
26
30
33

From the data you have collected you will need to produce two
graphs. One of the graphs will plot Osmotic Potential vs Time.
The other graph will plot Osmotic Change vs Time.
*

Answer the questions that follow your graphs about the
experiment you have performed.
*

You are now ready to begin part two of experiment #2.
In this part we will be concerned about a process related to
osmosis called Dialysis.
Glucose
Glucose
Starch
Starch
Lugol’s
Lugol’s
H2O
H2O

*
To magnify the blackboard click on it to view a discussion
about dialysis
Dialysis is the diffusion of a solute (salt, glucose, etc.) across a
selectively permeable membrane. In this case the solute
molecules always move from the stronger concentration
(hypertonic) to the weaker (hypotonic).

Dialysis involves the movement of some, but not all, of the
dissolved substances in a solution. The substance that moves
has small molecules, so these can pass through the pores in the
membrane, but other substances, with larger molecules, cannot
escape.

The green molecules are small enough to fit through the pores
of the semi permeable membrane while the red molecules are
too large.
*

This process occurs normally in the kidney. Substances with
small molecules, such as salts, glucose and urea, continuously
pass out of the blood through a membrane under pressure, but
useful substances are later reabsorbed. Waste substances are
then excreted as urine.
Kidney
*

In this exercise you will measure diffusion of small molecules
through dialysis tubing, an example of a semi permeable
membrane. The movement of a solute through a semi permeable
membrane is called dialysis. The size of the minute pores in the
dialysis tubing determines which substance can pass through the
membrane.

The light blue object here represents a section of dialysis
tubing. The red spheres represent molecules which are able to
move through the dialysis membrane, while the blue spheres
represent molecules too large to move out of the dialysis
membrane solution.
*

A solution of glucose and starch will be placed inside a bag of
dialysis tubing. Distilled water will be placed in a beaker,
outside the dialysis bag. The dialysis bag with the starch
glucose solution will be inserted into the beaker of distilled
water. After 30 minutes have passed, the solution inside the
dialysis tubing and the solution in the beaker will be tested for
glucose and starch. The presence of glucose will be tested with
glucose test strips. The presence of starch will be tested with
Lugol's solution (iodine potassium iodide).

*
PART 2 (DIALYSIS)
MATERIALS
30 cm section of dialysis tubing
String

15 % glucose solution
1 % starch solution
Glucose test strips
Lugol’s solution
Distilled water
2 Test tubes
Large beaker
*

Procedure
Obtain a 30 cm piece of dialysis tubing
Click on the tubing to obtain the dialysis tubing
Glucose
Glucose
Starch
Starch
H2O
H2O
Lugol’s
Lugol’s
Tubing
*

We will now tie one end of the dialysis tubing with the string.
Click on the string to tie the tubing
Glucose
Glucose
Starch
Starch
H2O
H2O
Lugol’s
Lugol’s
String

We can now place the dialysis tubing in distilled water to
moisten it so that it will be easier to open.
Click on the beaker of distilled water to bring it to the table
then click on the dialysis tubing to place the tubing in the
water.
Glucose
Glucose
Starch
Starch
H2O
Lugol’s
Lugol’s
H2O
*

While we let the dialysis tubing soak, we can access the glucose
and starch solutions.
Bring the two solutions to the table by clicking on either of
them.
Glucose
Starch
H2O
Lugol’s
Lugol’s
Starch
Glucose
Glucose
Starch

We will need to develop a method to test for glucose in a
solution as well as test a solution for starch.
Glucose
Starch
H2O
Lugol’s
Lugol’s
Starch
Glucose
*

You will need to record your data from this experiment on your
Data Table of Dialysis Test.
Glucose
Starch
H2O
Lugol’s
Lugol’s
Starch
Glucose

*
Glucose
Starch
H2O
Lugol’s
Lugol’s

Starch
Glucose
We will be using glucose test strips to test a solution for the
presence of glucose.
When the strip is exposed to glucose it will change color from
yellow to blue.
*

Glucose
Starch
H2O
Lugol’s
Lugol’s
Starch
Water
We will be using Lugol’s solution to test for the presence of
starch.
When Lugol’s solution is in the presence of starch the solution
will change to a dark blue color.

*
Glucose
Starch
H2O
Lugol’s
Lugol’s
Starch
Glucose
Click on the test strips to bring them to the table.
Next we will test the Glucose solution and the distilled water

where the dialysis tubing is now soaking for the presence of
glucose.
Test Strips
*

Glucose
Starch
H2O
Lugol’s
Lugol’s
Starch
Click on one of the glucose test strips.
You will be testing the glucose solution for the presence of
glucose.
As you have predicted, you will get a positive test. Record your
data.
Glucose
The color change on the strip shows a positive test for the
presence of glucose

*
Glucose

Starch
H2O
Lugol’s
Lugol’s
Starch
Click on another test strip to test the distilled water for the
presence of glucose.
Remember a positive test will turn the yellow end of the strip to
blue.
Record your results in your data table.
Glucose
Record your data
*

Glucose
Starch
H2O
Lugol’s
Lugol’s
Starch
Next we will test for starch in the starch solution and the
distilled water.
You will be using Lugol’s solution to test for starch.
If starch is present the solution will turn a dark blue color.
Glucose

*
Glucose
Starch
H2O

Lugol’s
Lugol’s
Starch
Click on the blue bottle of Lugol’s solution on the shelf to bring
it to the table.
You will need a test tube to test a small portion of the starch
solution.
Next click on the test tube rack.
Glucose
Test tube
*

Glucose
Starch
H2O
Lugol’s
Click on one of the eyedroppers on the shelf to add starch to the
test tube.
Glucose
Starch
Next click on the eyedropper in the bottle of Lugol’s solution to
add a few drops to the starch solution in the test tube.
Record your results on your data sheet.

Glucose
Starch
H2O
Lugol’s
Next we will test the distilled water for the presence of starch.
Click on the test tube rack to get a clean test tube
Glucose
Starch
*

Glucose
Starch
H2O
Lugol’s
Click on a fresh eyedropper from the shelf to add distilled water
to the test tube.
Glucose
Starch

Glucose
Starch
H2O
Lugol’s
Next click on the eyedropper in the Lugol’s solution to add a
few drops to test for starch.
Record your results.
Glucose
Starch
*

Glucose
Starch
H2O
Lugol’s
Next we will open the dialysis tubing and fill it with about ½
full of starch solution and about ½ full of glucose solution.
Leave enough of the tubing so that you can tie the end shut.
Glucose
Starch

Lugol’s
Click on the dialysis tubing in the distilled water beaker to
remove it so that it can be opened.
Next click on the starch or glucose solution to fill the dialysis
tubing.
Starch
Glucose
*

Glucose
Starch
H2O
Lugol’s
Next click on the yellow string to tie the top of the dialysis bag.
*

Glucose
Starch
H2O
Lugol’s
Now click on the dialysis bag to insert it into the distilled
water.
Leave the dialysis bag in the distilled water for 30 minutes.
Click the start button on the timer.
START
00:00

05:00
10:00
15:00
20:00
25:00
30:00
*

Glucose
Starch
H2O
Lugol’s
Remove the dialysis bag by clicking on it.
Open one end of the dialysis tubing by clicking on the yellow
string holding one end together

*
Glucose
Starch

H2O
Lugol’s
Click on the test tube rack to obtain a test tube.
We will add a small amount of solution from the dialysis tubing
to the test tube to test for glucose and starch.
*

Glucose
Starch
H2O
Lugol’s
Click on one of the blue eyedroppers on the shelf to add
solution from the dialysis tubing.
Next click on the one of the glucose test tapes to test for
glucose.
Record your results
*

Glucose
Starch
H2O
Lugol’s
Now we can test for starch by using a few drops of Lugol’s
solution.
Click on the eyedropper in the Lugol’s solution to add to the
dialysis tube solution.

Glucose
Starch
H2O
Lugol’s
We will now perform the same tests for glucose and starch on
the solution of distilled water in which the dialysis tubing
soaked for 30 minutes.
We will need a clean test tube. Click on the test tube rack.
*

Glucose
Starch
H2O
Lugol’s
Click on one of the blue eyedroppers on the shelf to add
solution from the distilled water.
Next click on the one of the glucose test tapes to test for
glucose.
Record your results

*
Glucose
Starch
H2O
Lugol’s
Now we can test for starch by using a few drops of Lugol’s
solution.
Click on the eyedropper in the Lugol’s solution to add to the
distilled water.

You are finished with part 2 of experiment # 2, be sure that you
answer the questions pertaining to this part of the experiment.
*

At this point you have finished experiment # 2, Parts 1 and 2 for
this lab.
End this Session
*

Diffusion Rate
CHEMICAL DIFFUSION IN AGAR
Click Here To Start Experiment #1

Diffusion is a process of equalization which involves movement
of molecules from an area of high concentration to an area of
low concentration

This exercise investigates diffusion as it applies to movement of
particles in a semi-solid material called agar

Molecules are in a constant state of motion
The motion of these molecules are influenced by many factors

1) Kinetic Energy
2) Nature of the environment
3) Size of the molecules

KINETIC ENERGY
The driving force which causes the molecules to move

Click Mouse Here to Perform the Exercise on Chemical
Diffusion in Agar
NATURE OF THE ENVIRONMENT
The agar environment we will be using is a semisolid material
through which we will measure the rate of diffusion of
substances with different molecular size

SIZE OF THE MOLECULES
Smaller molecules move faster than larger molecules

Click Mouse Here to Perform the Exercise on Chemical
Diffusion in Agar
You will need to supply the following in your lab review sheet
for experiment #1
1) Data Table for Diffusion of Different Molecular Weights
2) Answers to questions 1-3

ProcedureObtain two test tubes with agar
*You can obtain the test tubes by clicking on the test tube rack
on the shelf to the left
Test tube rack

1
2
Procedure
2) Mark the test tubes as
#1 and #2
*If you click on the pencil lying on the shelf to the left you can
mark the tubes

Procedure
3) Add a small amount of methylene blue to test tube #1
*You can click on the blue eyedropper lying on the shelf to the
left to add the dye
1
2

Procedure
4) Quickly click on the START button on the timer and record
on your printed Data Sheet every 15 minutes the distance the
dye has moved through the agar
*You can measure the progress of the dye by observing the mm
marks on the test tubes
1
2
START
00:00
05:00
10:00
15:00
20:00

25:00
30:00
35:00
40:00
45:00
50:00
55:00
60:00
Click here if you messed up and would like to try this
experiment again

Procedure
5) Add a small amount of potassium permanganate to test tube
#2
*You can click on the green eyedropper lying on the shelf to the
left to add the dye
1
2

Procedure
6) Quickly click on the START button on the timer placed on
the table and record every 15 minutes the distance the dye has
moved through the agar
*You can measure the progress of the dye by observing the mm
marks on the test tubes
1
2
START
00:00
05:00
10:00
15:00

20:00
25:00
30:00
35:00
40:00
45:00
50:00
55:00
60:00
Click here if you messed up and would like to try this
experiment again

DATA
At this point you should have collected diffusion distance data
for methylene blue and potassium permanganate
Complete the questions on your review sheet using the Data
Table for Diffusion of Different Molecular Weights

At this point you have finished experiment #1 for this lab
Continue to experiment #2 (OSMOSIS AND DIALYSIS)
NAME:__________________________ SECT:_________
EXPERIMENT #1: DIFFUSION RATE
Data Table for Diffusion of Different Molecular Weights
Methylene blue (molecular weight of 320)
Time 15 min 30 min 45 min 60 min
Distance in mm

Potassium permanganate (molecular weight of 158)
Time 15 min 30 min 45 min 60 min
Distance in mm
1) What are the three things which influence the movement of
molecules (and particles)? Circle
the variable that was measured in this experiment.
2) Which dye, the methylene blue or the potassium
permanganate, diffused at the fastest rate?
3) Why did one dye diffuse faster than the other?
EXPERIMENT #2, PART 1: OSMOSIS
Table of Osmotic Potential and Time
Time Osometer reading *Movement in mm
0 3 -
15 10 7

30
45
60
75
90
*To calculate movement in mm you will need to subtract the
preceding value from the
succeeding value
1) Over the 90 minute period, what distance did the column of
sugar move?
2) Which term is used to describe the tonicity of the distilled
water? (Hypotonic or Hypertonic)
3) Explain why the sugar solution rises in the thistle tube over
time.

4) Explain why the diffusion rate of water changed over the 90
minute period of time.
5) We began the experiment by pouring a 20% sucrose solution
into the thistle tube. Describe
what the makeup of the sucrose solution probably is after the 90
minute period. (more or less
than 20%).
6) Describe why this change in sucrose % has changed.
7) From the knowledge you have gained could you explain why
it is not a good idea to drink salt
water when you are thirsty.
EXPERIMENT #2, PART 2: DIALYSIS
TABLE OF DIALYSIS TESTS
Solution

Glucose Test Tape
(+/-)
Lugol’s

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