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Evaluation of harvesting and postharvest processing method on raw quality attributes of green Arabica Coffee beans produced in Hararghe,
eastern Ethiopia.
IJPBCS
Evaluation of harvesting and postharvest processing
method on raw quality attributes of green Arabica
Coffee beans produced in Hararghe, eastern Ethiopia
Mohammedsani Amin Ameyu1
*, Wassu Mohammed2
, Tesfaye Shimber3
1
Mechara Agricultural Research Center, Coffee Research Case Team, P. O. Box: 19, Mechara, Ethiopia
2
Haramaya University, School of Plant Sciences, P. O. Box: 138, Dire Dawa, Ethiopia
3
Ethiopian Institute of Agricultural Research, P. O. Box: 30708, Addis Ababa, Ethiopia
*Corresponding author’s E-mail: mamasaniamin@yahoo.com
The study was conducted to evaluate different harvesting and postharvest handling methods on
the inherent raw quality characteristics of Hararghe coffee. The experiment was designed as a
factorial combination of two harvesting (selective and strip harvesting) and six postharvest
processing (dry processed dried on bare, cemented and plastic sheet ground, and dry, wet and
semi-washed processed dried on mesh wire) methods in a Completely Randomized Design with
three replications. The samples were prepared from a Hararghe coffee genotype at Mechara.
Samples were evaluated for their raw quality attributes. The results indicated that the main
effect of harvesting method was highly significantly influenced all raw quality attributes whereas
the effect of postharvest processing methods was highly significantly influenced all raw quality
parameters, except odor of unwashed and all raw quality parameters of washed coffee. However
the two interaction effect showed significance variation in bean size. The selective harvesting
was better in producing superior quality beans in all parameters. Dry processed method
coupled with drying coffee on mesh wire was best in producing coffee beans with high raw
quality. In contrast, dry processing using bare ground produced inferior coffee for all raw
quality attributes. Wet processing showed superiority over semi-washed processing method.
Key words: Hararghe coffee, harvesting and postharvest practices, green coffee bean, raw quality attributes
INTRODUCTION
Coffee is the world’s most valuable and second most
traded agricultural commodity after oil in international
trade (ICO, 2007; Alemseged and Yeabsira, 2014). Today,
coffee is one of the most important non-alcoholic
beverage crops grown in over 80 countries and exported
in different forms to more than 165 nations of the world
(Dessalegn et al., 2008). It is used as a source of income
to several developing countries in Africa, Asia and Latin
America (Alemseged and Yeabsira, 2014). Among the 103
diverse species of genus Coffea (Davis et al., 2006), only
two species namely Arabica (Coffea arabica L.) and
robusta (Coffea canephora Pierre) are under commercial
cultivation (Anthony et al., 2002; Pearl et al., 2004). The
center of origin of the genus Coffea is geographically
isolated from the center of origin of Arabaica coffee which
is believed to the plateau of southwestern Ethiopia
(Anthony et al., 2002; Steiger et al., 2002), the former
Limmu province of Jimma, Limmu Kossa district, chorra
village.
As the origin of all origins, Ethiopia has another unique
feature for the hundreds of heirloom varietals. In many
cases, farmers grow their own unique heirloom varietals,
the majority of which grow else in the world (Boot, 2011).
For example, among the several renowned types of coffee
known with very fine quality coffee in Ethiopia Hararghe
(former Harar), Sidama and Yirgachefe coffees are well
known in contributing their own special quality coffee
International Journal of Plant Breeding and Crop Science
Vol. 4(2), pp. 187-196, April, 2017. © www.premierpublishers.org. ISSN: 2167-0449
Research Article
Ameyu et al. 188
acclaimed for its aroma and flavor characteristics to the
world (Boot, 2011; Emebet et al., 2013). Currently these
coffee types are registered for trademark in 27 European
countries, USA, India, Japan, Canada, Saudi Arabia,
China and South Africa (Emebet et al., 2013). Thus, they
are sold at a premium price both at domestic and
international coffee markets (ITC, 2002).
The region known as Hararghe comprises the eastern
most of the coffee growing regions of Ethiopia. It is
subdivided into east and west Hararghe zone (Boot,
2011). Coffee produced in this region is a Coffea arabica
species growing in the highland and midland areas of the
zones and well recognized specialty and exemplified
category coffee which is grown mostly without shade
(Desse, 2008). It is characterized by medium sized beans
with greenish yellow color, medium acidity and full body
and a distinctive mocha flavor. Quality of Hararghe coffees
are notable for a fruity characteristic and have a distinct
note of blueberry, though many other fruity and fruit-like
aromatic flavors can occur. Furthermore, it can be
differentiated by its amber or golden bean coffee (Boot,
2011). Internationally it is known and recognized as Harar
trade brand name and highest premium coffee in the world
(EMT, 2012). Therefore, this distinctive fine quality coffee
type of this region is currently known by the name of
specialty coffee in international market. Among the
Hararghe (Harar) coffee, east Hararghe coffees tend to
have the most distinct blueberry flavors as the result of a
certain varietal growing in a certain soil type in East
Hararghe. This is “naturally occurring” amber bean coffee,
and often has an excellent, vibrant and rich blueberry
flavor. However, often west Hararghe coffees have a
thicker, smoother body, and a milder fruit flavor (Boot,
2011). Interms of production quantity west Hararghe zone
is contributing about 65% of Hararghe coffees.
As a foreign exchange earner Hararghe coffee is exported
to all over the world, but there is a particular demand for it
in Saudi Arabia. This constant demand and its premium
quality tend to keep the price for commercial grade
Hararghe coffee slightly higher than most other Ethiopian
coffee regions (Boot, 2011). However, merely having such
potential will not bring significant contribution to country’s
income unless and otherwise the products meet the
demand of customers in terms quality. This mainly
requires the improvement of agronomic practices such as
harvesting, processing and handling practices. Most
farmers in the area harvest coffee by allowing more
cherries to mature, immature, some to dry, some to fallen
on the ground and some cherries are unripe on the mother
tree. They do not practice the recommended selective
picking. The main reason discourages farmers not to
harvest and process their coffee with great care is
absence of premium price for good quality harvest and
drying coffees at primarily transaction center
(Mohammedsani, 2014). Additionally, in Hararghe only
natural sundried method has been used in which coffee
cherries are harvested once from the tree and dried on
plastic sheet or bare ground (Boot, 2011; Mohammedsani,
2014). These indicate that, the deterioration of physical
bean quality of Hararghe coffee. Related to this Desse
(2008) reported that, poor harvesting practices such as
stripping and collecting dropped fruits from the ground and
improper postharvest handling practices such as bad
processing and drying on bare ground have contributed a
significant share for the production of low quality of green
coffee bean. Particularly, in Hararghe coffee raw bean
quality is declining from time to time due to poor
harvesting and postharvest processing practices
(Mohammedsani, 2014).
This adverse situation has been largely attributed to the
unsatisfactory quality of the coffee beans rising from poor
harvesting and processing methods (Mutua, 2000)
attempted to gain some insight into the resolution of the
problem. Thus, there is a strong interest for this region of
coffee in producing and marketing coffee of higher quality
to alleviate financial difficulties encountered by coffee
farmers (Behailu et al., 2008). With these reasons, the first
priority in research is on coffee quality, paying much
attention to quality improvement and maintenance in the
coffee growing regions. Hence, in order to produce and
supply Hararghe coffee bean with its inherent quality
characteristics, it is necessary to conduct research on this
first research priority topic that have never been studied in
the areas. Therefore, this study was initiated with the
specific objective to evaluate the effects of harvesting and
postharvest processing methods on raw quality attributes
of a Hararghe coffee genotype.
MATERIALS AND METHODS
Description of the study area
The experiment was conducted at Mechara Agricultural
Research Center (MCARC) in 2013 cropping season on
young established Hararghe coffee genotype. The center
is located near Mechara town in Darolabu district of West
Hararge Zone, eastern Ethiopia. It is 434 km east of Addis
Ababa, the capital city and 115 km southeast of Chiro
town, the zonal capital. Geographically, it is located
between latitude of 40o
19’29" North and longitude of
8
o
36'38" East at an altitude of 1760 m.a.s.l. The soil of the
center is sandy clay loam that is suitable for Arabica
coffee production (McARC, 2015). The area receives an
average annual rainfall of 1100 mm with high variability in
the onset and cessation of the main rainfall season. Its
annual mean minimum and maximum air temperatures
are 14
o
C and 26
o
C, respectively.
Experimental material
One promising Hararghe coffee selection namely H-
622/98 was used from coffee variety verification research
trial started in 2005. The selected genotype was
previously collected from Gemmechis district (Chirkane
Int. J. Plant Breeding and Crop Sci. 189
site) of west Hararghe zone in 1998 and planted at
Mechara on station in July 2005. It is among the top
yielding genotype from the 14 promising Hararghe coffee
selections under evaluation. Regarding its bean quality
characteristics, good flavor note is scored for this
genotype which shows the typical quality profile of
Hararghe coffee (Abdi et al., 2011).
Experimental design
The treatments were arranged using factorial arrangement
in a completely randomized design with three replications.
Accordingly, the treatments consists of two types of coffee
harvesting methods viz.; strip harvesting and selective
picking were combined with six types of postharvest
processing methods viz.; dry processing drying on bare
ground, dry processing drying on cemented ground, dry
processing drying on plastic sheet ground, dry processing
drying on mesh wire, semi-washed processing and wet
processing drying on mesh wire.
Experimental procedures
Coffee harvesting
Coffee cherries were harvested following both harvesting
methods (strip harvesting and selective picking). In these
methods, cherries were harvested at different times. This
is because under strip harvesting, cherries were harvested
once at a time when ripe red, turned red, immature green
and turned dried cherries (when 75% of the cherries were
reached at full ripe) whereas selective picking required
more than one harvesting since only red ripe cherries
were harvested as they attained this stage. For this, fruit
bearing coffee trees were assigned for the two harvesting
treatments in such a way that the odd and even number
coffee trees were used for strip and selective harvesting
methods, respectively. Consequently, harvesting of the
coffee cherries was done from October to December
2013. Finally, all cherries harvested by each harvesting
method was passed to different postharvest processing
methods.
Dry processing
All cherries harvested using both harvesting methods
were immediately spread out to dry in the sun using four
drying methods (bare, cemented and plastic sheet ground
and raised mesh wire table). They were stirred regularly to
promote even drying, prevent fermentation and the
development of mold in each treatment. All sample
cherries were dried up to when their outer shell skin
became dark brown and brittle (Clark, 1985; Boot, 2011)
or when their approximate moisture content attained
11.5%. Finally dried coffee cherries were collected and
hulled with mortar as farmers are practicing carefully and
cleaned (ISO, 2004).
Wet processing
Under this processing method the cherries were pulped
using single disc hand pulper separately as per the
harvesting method. The pulped cherries were collected
inside the large size plastic buckets where pulps and
floater parchments bean were removed. Subsequently,
the wet parchment beans were transferred into other
bucket and fresh water was added on it until parchment
beans were totally submerged inside the water for
fermentation. They were fermented for 40 hours (Woelore,
1993) during which the water was changed three times.
When the mucilage was totally degraded, parchment
coffee beans were washed intensively for the total
removal of mucilage (Boot, 2011). The resulting green
parchment beans were prepared and allowed to dry under
full sun condition on 80cm above ground raised mesh wire
table (Behailu et al., 2008) until approximately their
moisture content attained 11.5%. Finally, the samples
were slowly hulled and hand polished to remove the
parchment and silver skins from green coffee beans (ISO,
2004).
Semi-washed processing
Similar to wet processing method, the harvested cherries
were pulped with single disc hand pulper and collected in
large size plastic buckets. However, as opposed to it,
parchment beans were immediately washed manually and
rubbed with canavas cloth by hand until the mucilage was
clearly removed from the parchment beans then after the
parchment beans were transferred to above ground mesh
wire drying table under full sun conditions (ES, 2001).
Finally, similar to wet processing method the parchment
beans were prepared for raw quality evaluation.
Laboratory analysis
All samples prepared by using the above harvesting and
processing methods were assigned an arbitrary code (an
identity letter and number) and brought to coffee quality
laboratory of Jimma Agricultural Research Center where
the green coffee beans were evaluated for different raw
quality attributes. The moisture content of the sample was
checked using Electronic Rapid Moisture Tester (HE 50,
Germany) to make the uniform required moisture level of
all samples.
Bean defect collection
Currently, Ethiopian Commodity Exchange (ECX)
evaluated raw coffee bean quality with bean defect
(primary and secondary defect), odor, color, shape and
make as raw quality attributes of green bean in Ethiopia
(ECX, 2010). Accordingly, 350 g of green coffee bean
sample from each treatment combination was used for
defect green bean quality evaluation. Thus, as per ECX
(2010) evaluation standard, green coffee beans were
evaluated for bean defect by counting primary defect and
weighting secondary defects. Defects like full black, full
sour, fungus attacked, foreign matter and insect damaged
beans were considered as primary defects whereas partial
black, partial sour, floater, immature, withered, shell,
slightly insect damaged, foxy, under dried, over dried,
Ameyu et al. 190
mixed dried, stinkers, faded, coated, light and starved
beans were identified as type of secondary defects.
Data collection
Bean size: it is the size of coffee beans which was
determined by the conventional screen analysis in which
the weight fractions retained on sieve of screen 14 with
respective hole diameter of 5.55 mm were recorded and
percentage was calculated and used for data analysis.
Bean weight: hundred (100) beans were randomly
counted from each 350 g sample and their weight was
measured in gram using a two digit sensitive balance.
Primary defect (counted): different types of coffee bean
defects recognized as full black, full sour, fungus attacked,
foreign matter and insect damaged were counted from
350 g of green beans sample and the percentage of total
defect number was calculated and scored out of 15% for
unwashed and 10% for washed coffee bean
Secondary defect (weighted): other bean defect type
including partial black, partial sour, floater, immature,
withered, shell, slightly insect damaged, foxy, under dried,
over dried, mixed dried, stinkers, faded, coated, light and
starved were collected together from 350 g of green beans
and weighed by using a sensitive balance. The
percentage of total defect weight was calculated and
scored out of 15% for unwashed and 10% for washed
coffee bean.
Shape and make: it is the structural make up of different
kinds of beans. It was evaluated out of score 10% only for
washed coffee as very good, good, fairly good, average,
fair and small and weighed accordingly (ECX, 2010).
Color: it is the overall physical appearance of coffee beans
that was evaluated out of score 5% only for washed coffee
as bluish, grayish, greenish, coated, faded and white
(ECX, 2010) and weighed accordingly.
Odor: it is the olfaction of coffee beans which was
evaluated out of score 10% for unwashed and 5% for
washed coffee as clean, fairly clean, trace, light, moderate
and strong (ECX, 2010).
Data analysis
Data were subjected to analysis of variance (two way
ANOVA) using general linear model (GLM) procedure of
Statistical Software Program (SAS 9.2). Whenever
ANOVA showed significant variation, LSD at 5%
probability level was used for treatment mean separation.
RESULTS AND DISCUSSION
Bean size
Coffee harvesting methods resulted significant (P≤0.01)
variation on bean size, while the effect of postharvest
processing methods had no significant effect on bean
size. This shows processing did not change the size of the
bean since it processes bean as it is. However, the
interaction effect of harvesting methods by postharvest
processing methods showed significant (P≤0.05) variation
on bean size. The interaction effect of coffee harvesting
and postharvest processing methods on total percentage
of bean size retained above screen size 14 ranged from
84% to 93% for selective harvesting and the dry
processed dried on cemented ground and semi-washed
processed dried on mesh wire, respectively (Table 1). This
depicted that coffee beans harvested by selective picking
and treated with different postharvest processing methods
can meet export standards. In line with this the finding by
Mekonen (2009) indicated that, selective harvested coffee
of different varieties showed significant variation in bean
size by recording highest percentage of beans retained
above screen.
However, postharvest processing methods combined with
strip harvesting ranged from 69 to 76% for washed and
unwashed coffee dried on mesh wire and bare ground,
respectively. These can fall under category of rejected
commercial coffee according to ECX (2010) standard, in
which any Ethiopian export coffee shall have the minimum
85% of bean weight remaining on top of screen 14. This
could be due to the effect of collecting different type of
fruits viz; immature, mature, red, dried, small, and big size
beans. These all together might produce the size of the
bean with less uniformity and low percentage of beans
below the required screen size. Similarly, Anteneh (2011)
reported that, poor harvesting practices, such as stripping
different type of fruits together, reduces quality and
increases losses by promoting uneven distribution of bean
size. In contrast, selective harvesting of red fruits
produced uniform bean size that is above the minimum
required bean screen size. This in agreement with the
finding of Bertrand et al. (2006), who suggested that for C.
arabica, picking of red cherries at appropriate maturity
stage, gives the best coffee bean size. Similarly, ITC
(2002) reported that the quality of a bean size comes from
care taken during harvesting.
In general, the percentage of green bean size decreased
from selective harvesting (93%) to strip harvesting (69%)
combined with semi-washed processing and drying on
raised mesh wire table, respectively (Table 1). This is
because in contrast to selective harvesting, for strip
harvesting there was no any sorting practice rather the
beans were directly taken to processing that could
increase the number of under sized bean coming from
striping of immature and defective cherries. Hence, sorting
under size beans can increase percentage of beans
retained above screen size 14, though it incurs additional
costs. Currently, coffee traders often practice this before
transporting it to central market for quality assurance and
grading in order to improve quality of coffee and economic
return from export market. This support with result of
Anteneh (2011) who reported that in order to improve
Int. J. Plant Breeding and Crop Sci. 191
Table 1. Interaction effect of coffee harvesting and postharvest processing methods on bean size above screen size of 14
at Mechara
Harvesting Methods Postharvest Processing Methods % of bean screen size
Strip harvesting
Dry processed dried on bare ground 76.00
c
Dry processed dried on cemented ground 73.83
cd
Dry processed dried on plastic sheet ground 74.70
cd
Dry processed dried on mesh wire 73.77
cd
Semi-washed processed dried on mesh wire 69.20
d
Wet processed dried on mesh wire 69.83
cd
Selective harvesting
Dry processed dried on bare ground 87.90
ab
Dry processed dried on cemented ground 84.33
b
Dry processed dried on plastic sheet ground 89.00
ab
Dry processed dried on mesh wire 85.87
b
Semi-washed processed dried on mesh wire 93.70
a
Wet processed dried on mesh wire 90.53
ab
LSD (5%) 6.36
C.V (%) 4.70
Mean 80.72
Mean values followed by the same letter (s) within a column are not significantly different at P≤0.05 level of significance.
quality of Hararghe coffee, traders practice some value
adding activities like removing defective and under sized
beans through sorting and cleaning.
From the present finding, the uniform, bold and heavy
coffee beans were obtained from selective harvesting
coffee treated under different postharvest processing
methods. Besides, this result perhaps attributable due to
the genetic characteristics of Hararghe coffee that known
by medium bean size that have acceptability in history of
Ethiopian coffee market and also good growth habit of this
genotype may contribute to the present finding in
production of medium to large bean size. This would
improve final raw and cup quality of the beans and mostly
preferred by roasters to pay premium price. According to
Agwanda et al. (2003), bean physical characteristics such
as bean size are unified criteria for conducting coffee
business within the international market. Similarly, bold
and medium bean size has a particular importance to
roasters, as uniform bean size would produce uniform
roast (Yigzaw, 2005; EAFCA, 2008). Additionally, Barel
and Jacquet (1994) supported that, roasting uneven sized
beans the smallest tend to burn and the largest tend to be
under-roasted, this tend to reduce final quality of the brew.
Bean weight
The results indicated highly significant variation in 100
bean weight due to the main effect of harvesting and
postharvest processing methods. But the interactions did
not significantly affect 100-bean weight. As a result, higher
value was recorded from selective harvesting method
compared to strip harvesting (Table 2). This might be due
to collection of different stage and sized fruits together at
once during harvesting which not only affect coffee bean
size but also bean weight of the coffee. ITC (2011) also
reported that, picking of immature cherry with mature
cherries could cause the reduction of weight of the beans.
In conformity with this Boot (2006) reported that under
almost all conditions, the specific weight of a ripe cherry is
greater than that of an immature cherry, it is heavier,
weighing up to 20% more.
Similarly, processing methods highly differed and
produced an average of 100-bean weight ranging from
12.43 g to 14.97 g. Dry processed and dried on different
methods showed light to medium beans and wet
processed had medium to heavy beans with mean value
of 14.97 g per 100-beans (Table 2). This result generally
revealed that dry processed coffee with different
postharvest practice produced bean weight lighter than
that of wet processed coffee. This is because under dry
processed coffee all harvested cherries (mature and
immature) were directly spread to full sun without sorting
and clearing as opposed to washed processed in which
sorting of abnormal, immature and floaters were
performed. In agreement with this, Mekonen (2009) found
out that significant difference in bean weight due to effect
of processing methods in which dry processed coffee had
lower weight than wet processed because of the removal
of immature and immersed fruits in washed processing.
Primary defect
The effect of harvesting and postharvest processing
methods was highly significant variation on primary defect
in unwashed or dry processed coffee. Likewise, harvesting
methods significantly (P≤0.01) influenced the primary
Ameyu et al. 192
Table 2. Main effect of coffee harvesting and postharvest processing methods on 100-bean weight of promising
Hararghe coffee genotype at Mechara
Treatments 100-Bean Weight (g)
Harvesting Methods
Strip harvesting 13.24
b
Selective harvesting 14.97
a
LSD (5%) 0.34
Postharvest Processing Methods
Dry processed dried on bare ground 12.43
d
Dry processed dried on cemented ground 13.48
c
Dry processed dried on plastic sheet ground 14.40
b
Dry processed dried on mesh wire 14.57
ab
Semi-washed processed dried on mesh wire 14.78
ab
Wet processed dried on mesh wire 14.97
a
LSD (5%) 0.59
C.V (%) 3.50
Mean 14.11
Mean values followed by the same letter (s) within a column are not significantly different at P≤0.05 level of
significance
Table 3. Raw quality attributes of natural sundried coffee as affected by main effect of harvesting and dry
processing methods
Treatments Raw quality parameter
PD (%) SD (%) Odor (%)
Harvesting methods
Strip harvesting 3.25
b
6.62
b
8.56
b
Selective harvesting 14.00
a
12.75
a
9.06
a
LSD (5%) 1.78 1.82 0.49
Postharvest processing methods
Dry processed dried on bare ground 6.25
b
6.75
b
8.42
Dry processed dried on cemented ground 8.50
b
9.50
a
8.67
Dry processed dried on plastic sheet ground 8.50
b
10.50
a
9.08
Dry processed dried on mesh wire 11.25
a
12.00
a
9.08
LSD (5%) 2.51 2.57 NS
CV (%) 23.80 21.70 6.50
Mean 8.62 9.69 8.81
Mean values followed by the same letter within columns are not significantly different at P≤0.05 level of significance;
PD=Primary defect; SD=Secondary defect; NS= Non-significant
defect, but postharvest processing methods did not
influence in washed coffee processing. Similarly, the
combined effect of harvesting and processing methods did
not significantly influence primary defect in both washed
and unwashed coffee.
The lowest average numbers of primary defects were
counted with mean value of 14% out of 15% in unwashed
coffee (Table 3) and 9.67% out of 10% in washed coffee
(Table 4) for selective harvesting. In contrast, beans from
strip-harvested coffee recorded the highest number of
primary defect with mean value of 3.25% in unwashed
coffee (Table 3) and 6.67% in washed coffee (Table 4).
From each treatment, only few numbers of defects like full
black, fungus attacked and insect damage were recorded
as primary defects in selective harvesting, indicating
reduced problems of coffee harvested with selective red
ripe cherries gave beans free from primary defect in both
processing methods. Similarly, Elsa et al. (2015) found
that the probability of coffee varieties free from any
primary defect with mean value of 15% was due to proper
harvesting of red ripe cherries followed by proper
postharvest handling. Moreover, the result revealed that
strip harvesting produced coffee beans with high number
Int. J. Plant Breeding and Crop Sci. 193
Table 4. Raw coffee quality attributes of washed coffee as affected by main effect of coffee harvesting and postharvest
processing methods
Harvesting Methods
Raw quality attributes
PD (%) SD (%) Odor (%) SM (%) Color (%)
Strip harvesting 6.67
b
3.67
b
4.29
b
6.05
b
3.17
b
Selective harvesting 9.67
a
9.33
a
4.88
a
8.14
a
4.18
a
LSD (5%) 1.72 1.54 0.21 0.43 0.24
Postharvest processing methods
Semi-washed processed dried on mesh wire 8.33 6.00 4.56 7.16 3.65
Wet processed dried on mesh wire 8.00 7.00 4.61 7.03 3.70
LSD (5%) NS NS NS NS NS
C.V (%) 15.80 17.80 4.20 4.50 3.90
Mean 8.17 6.5 3.67 7.10 4.58
Mean values followed by the same letter within columns are not significantly different at P≤0.05 level of significance;
NS=Non-significant; PD=Primary defect; SD=Secondary defect; SM=Shape and make
of defects which may be due to harvesting of insect and
fungus damaged fruits. This was in agreement with the
finding of Barel and Jacquet (1994), who reported
traditional selective hand pricking as opposed to strip
harvest, produced the best quality green coffee by
decreasing the percentage of defects in coffee batches.
With regard to effect of dry processing on primary defect,
the highest (11.25%) and the lowest (6.25%) mean values
were produced for cherries dried on mesh wire and bare
ground, respectively. However, coffee cherries dried on
plastic sheet and cemented ground had the same value of
6.50% (Table 3). This might be due to the fact that, fruits
dried on bare, plastic sheet and cemented floor had
contact with foreign matter, exposed to re-wetting of
cherries and less air movement during drying than the
raised mesh wire. This could favor development of mold
as well as black bean formation. This in turn might have
increased the number of primary defect counts that can
affect final physical appearance of the beans. The results
support Enyan et al. (2013) who pointed out that the
drying of coffee beans on the concrete floor and black
polythene sheet on concrete floor resulted in a higher
percentage of defective beans than on the raised raffia
plastic sheet. The authors additionally reported that insect
damage and black beans constituted more than 70% of
the defective beans, related to the processing methods
used.
This is in line with Berhanu et al. (2014) who reported that,
inappropriate postharvest management practices increase
the foxy bean formation that maximizes the degree of
defect counts and affects the odor and color of the coffee
that finally affects the raw quality of green beans.
Similarly, the present study showed that the presence of
defects in coffee was indicative of its reduced quality that
translated into price and acceptability of the coffee on the
commodity market. Negussie et al. (2009) also pointed out
that properly processed coffee is free of off-flavor with few
defective beans.
Secondary defect
The result from analysis of variance showed that, there
was a highly significant variation in secondary defects due
to main effect of harvesting and postharvest processing
methods for unwashed coffee. Similarly, the effect of
harvesting methods was significant (P≤0.01) but not for
postharvest processing methods that showed non-
significant variation in washed coffee. However, the
interaction effect of harvesting and postharvest processing
methods did not significantly affect secondary defect in
both dry and washed coffees.
Selective harvesting had high mean value of 12.75%
indicating relatively pure coffee bean. However, the lower
mean value (3.23%) was recorded from strip harvesting,
which indicates high number of secondary defect in poorly
harvested and unwashed coffee (Table 3). Similarly, in
washed coffee, the highest mean value (9.33%) of
secondary defect was recorded for selective harvesting
and the lowest (3.33%) for strip harvesting (Table 4).
These showed that selective harvesting produced coffee
beans free from secondary defects as compared to strip
harvesting in dry and wet processed coffee. This is
because selective harvesting involves only picking of the
red, fully ripe and normal cherries carefully from tree,
while in strip harvesting collecting of entire crop
(immature, mature, ripe, over mature, dried, insect
damaged) just by one pass through cropping season. This
result is in line with Hicks (2002) who described that
although selective picking is more expensive, but it can
produce the best results of coffee by reducing number of
defects thereby increase overall quality of coffee that are
competent in the world market.
Moreover, coffee processed and dried with different
methods showed significant variation on secondary
defects. Hence, the highest mean value (12%) was
recorded for dry processed coffee and dried on mesh wire,
while the lowest value (6.75%) of secondary defect was
Ameyu et al. 194
recorded in dry processed and dried on bare ground
followed by dried on cemented and plastic sheet ground
(Table 3). The present finding agreed with Olamcam
(2008) result where coffee that is well harvested and
properly processed has no or very few broken beans and
free of foreign matter (stick, stones, and leaves). Hicks
(2002) identified that coffee that has been inappropriately
dried would become brittle and produce too many broken
beans that considered as secondary defect during hulling.
Among the secondary defect observed in this study, the
major ones are partial black, foxy, immature, and slightly
insect damaged beans, coated, broken, shell and faded
beans that have quality deteriorating effects.
Odor
The analysis of variance for unwashed coffee revealed
significant variation (P≤0.05) on odor due to the main
effect of coffee harvesting and did not affected by
postharvest processing methods. In washed coffee, this
was highly significant for harvesting methods, but did not
differ due to postharvest processing methods. However, in
both unwashed and washed coffee, the interaction effect
showed non-significant variations on odor.
In both harvesting methods, comparable higher mean
values of odor (>8%) were recorded. However, for
selective harvesting the mean values of odor was higher
than strip harvesting in unwashed coffee (Table 3). All
beans had odor point ranging from 8 to 9% which can be
categorized as fair clean to clean (ECX, 2010). These
showed that green coffee smell was affected due to
improper harvesting method. This is in line with the finding
of Olamcam (2008) who explained that, properly
harvested and processed beans are free of unpleasant
(bad) smells. In accordance with this result, Endale (2008)
reported that, coffee with better management in each
stage starting from harvesting until cupping turn out to
have better odor.
On the other hand, in washed coffee higher mean value
above four of odor was recorded for selective harvesting
method, which can be categorized under clean odor
(Table 4). In general, unwashed coffee had fair clean odor
and washed coffee had clean odor. Likewise, Anwar
(2010) reported that wet processing methods resulted in
high mean values (9.04%) as compared to dry processing
methods (7.48%) for odor.
Shape and make
Coffee beans significantly (P≤0.01) varied for shape and
make due to main effect of harvesting methods, but not
significantly affected by postharvest processing methods
and the interaction. The highest average value of 8.14%
was recorded for selective harvested coffees which
possessed a very good shape and make with more
uniform appearance (Table 4). Correspondingly, Mekonen
(2009) reported the selectively picked red fruits of different
coffee accessions have very good shape and make under
both wet and dry processing. In contrast, the lower shape
and make (6.04%) was recorded from strip-harvested
coffees indicating fairly good shape and make with small
bean size (Table 4).This perhaps is due to the mixing of
mature, immature, under sized and over fermented (on
mother tree dried) cherries during strip harvesting that
could produce poor appearance beans thereby reduce the
flavor and acidity of coffee. In connection with this
Anteneh (2011) reported that, either mixing defective
beans during harvesting by producers was the main
reasons for deteriorating coffee physical appearance.
However, Wintgens (2004) indicated that the shape and
structure of beans are the result of both genotype and
environmental factors.
Color
Green coffee bean color was significantly (P≤0.01)
influenced by harvesting methods, however, both
postharvest processing and interaction effects of
harvesting and postharvest processing did not influence
bean color of washed and semi-washed coffee. The result
was as opposed to the result of Mekonen (2009) who
reported high significant variations among coffees
processed in different processing methods. This may be
due to the unique genetic characteristics of a Hararghe
coffee genotype that can maintain its bean color in any
processing method.
Thus, higher mean value (4.10%) was recorded for
selective harvested coffees as compared to striped coffee
beans (Table 4). This might be due to the mixture of
harvesting of different fruits together at once. This agreed
with the report of ITC (2002) in that uneven color of the
beans revealed from mixing of immature, overripe and ripe
coffee cherries during harvesting. Anwar (2010) also
found that if coffee is harvested before the beans are ripe
or at immature stage, the end product will show color
defect. Similarly, Olamcam (2008) reported that, well
harvested and processed coffee had clean and uniform
color. Furthermore, uneven bean color can be from poor
processing, incorrect moisture content during the
fermentation, premature aging of the coffee, poor drying
techniques, harvesting of overripe cherries, keeping
cherries a long time before pulping and/or insufficient
washing after natural fermentation (ITC, 2002).
CONCLUSION
Improper harvesting and postharvest processing are
adversely affecting quality of the coffee produced in
Hararghe region. Taking these problems into account, this
study was conducted to evaluate the effect of harvesting
and postharvest processing methods on raw quality of a
Hararghe coffee genotype. For the study, the coffee
cherry samples were prepared from one young
established Hararghe coffee selection planted at the
Mechara. The evaluation for raw appearance of green
bean was conducted in the coffee quality laboratory.
Hence, the finding indicated that, bean size was
Int. J. Plant Breeding and Crop Sci. 195
significantly affected by harvesting and the interaction of
harvesting and postharvest processing methods while,
bean weight was affected highly by both harvesting and
postharvest processing methods. Coffee beans harvested
by selective harvesting and treated under different
postharvest processing methods had 85% and above the
minimum required bean size for export coffee as
compared to strip harvested beans in which all beans are
recorded under rejected coffee due to a number of small
beans (<76%). Similarly, weight of 100-beans was higher
for coffee samples harvested by selective harvesting as
compared to strip harvesting. Primary and secondary
defects and odor of both unwashed and washed coffee as
well as color, shape and make of washed coffees were
highly significantly influenced by harvesting methods.
Selective harvesting produced beans with high raw quality
of all attributes in both washed and unwashed coffee.
Postharvest processing treatment highly significantly
influenced all raw quality attributes except odor of
unwashed coffee but these were not significantly affected
in washed coffees. Natural sundried coffee on raised
mesh wire was better in producing high raw bean quality
with the lowest primary and secondary defects followed by
coffee dried on plastic sheet ground floor. In general, the
overall findings of the present study indicated that strip
harvesting as practiced by many Hararghe coffee
producers has a determinant effect on raw and physical
appearance of coffee quality in the Hararghe areas. This
showed that if producers avoid stripping cherries and
practiced selective picking of red cherries only, the quality
of coffee could be improved. Moreover, drying coffee on
bare ground highly reduced raw, abnormal color and
unpleasant odor. Therefore, proper harvesting and drying
practices were found to be crucial in maintaining the
typical inherent quality characteristics of Hararghe coffee
beans.
ACKNOWLEDGEMENTS
The authors would like to thank the Oromia Agricultural
Research Institute (OARI) for sponsoring this research
work and Mechara Agricultural Research Center (McARC)
for facilitating the wok on the field at Mechara main
research station. Further, we wish to convey our deepest
thanks to all the McARC’s coffee research case team
staff, namely, Ahmed Aliyi, Atika Eliyas, Sorse Beyissa,
Hika Bersisa, Mulugeta Mamo and Assefa Juhar for their
technical support as a group during filed sample
preparation. Lastly but not the least we would also like to
acknowledge all the Jimma Agricultural Research Center
coffee processing and quality research staff namely, Mr.
Abrar Sualeh, Nigussie Mekonen, Mikru Tesfa, Wabi Bajo,
Dasse Baley and Murad Abraya and Miss Saida Abdu and
Foziya Kemal for their willingness to host and sharing their
experiences, supporting and facilitating data collection in
the laboratory.
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Evaluation of harvesting and postharvest processing method on raw quality attributes of green Arabica Coffee beans produced in Hararghe, eastern Ethiopia

  • 1. Evaluation of harvesting and postharvest processing method on raw quality attributes of green Arabica Coffee beans produced in Hararghe, eastern Ethiopia. IJPBCS Evaluation of harvesting and postharvest processing method on raw quality attributes of green Arabica Coffee beans produced in Hararghe, eastern Ethiopia Mohammedsani Amin Ameyu1 *, Wassu Mohammed2 , Tesfaye Shimber3 1 Mechara Agricultural Research Center, Coffee Research Case Team, P. O. Box: 19, Mechara, Ethiopia 2 Haramaya University, School of Plant Sciences, P. O. Box: 138, Dire Dawa, Ethiopia 3 Ethiopian Institute of Agricultural Research, P. O. Box: 30708, Addis Ababa, Ethiopia *Corresponding author’s E-mail: mamasaniamin@yahoo.com The study was conducted to evaluate different harvesting and postharvest handling methods on the inherent raw quality characteristics of Hararghe coffee. The experiment was designed as a factorial combination of two harvesting (selective and strip harvesting) and six postharvest processing (dry processed dried on bare, cemented and plastic sheet ground, and dry, wet and semi-washed processed dried on mesh wire) methods in a Completely Randomized Design with three replications. The samples were prepared from a Hararghe coffee genotype at Mechara. Samples were evaluated for their raw quality attributes. The results indicated that the main effect of harvesting method was highly significantly influenced all raw quality attributes whereas the effect of postharvest processing methods was highly significantly influenced all raw quality parameters, except odor of unwashed and all raw quality parameters of washed coffee. However the two interaction effect showed significance variation in bean size. The selective harvesting was better in producing superior quality beans in all parameters. Dry processed method coupled with drying coffee on mesh wire was best in producing coffee beans with high raw quality. In contrast, dry processing using bare ground produced inferior coffee for all raw quality attributes. Wet processing showed superiority over semi-washed processing method. Key words: Hararghe coffee, harvesting and postharvest practices, green coffee bean, raw quality attributes INTRODUCTION Coffee is the world’s most valuable and second most traded agricultural commodity after oil in international trade (ICO, 2007; Alemseged and Yeabsira, 2014). Today, coffee is one of the most important non-alcoholic beverage crops grown in over 80 countries and exported in different forms to more than 165 nations of the world (Dessalegn et al., 2008). It is used as a source of income to several developing countries in Africa, Asia and Latin America (Alemseged and Yeabsira, 2014). Among the 103 diverse species of genus Coffea (Davis et al., 2006), only two species namely Arabica (Coffea arabica L.) and robusta (Coffea canephora Pierre) are under commercial cultivation (Anthony et al., 2002; Pearl et al., 2004). The center of origin of the genus Coffea is geographically isolated from the center of origin of Arabaica coffee which is believed to the plateau of southwestern Ethiopia (Anthony et al., 2002; Steiger et al., 2002), the former Limmu province of Jimma, Limmu Kossa district, chorra village. As the origin of all origins, Ethiopia has another unique feature for the hundreds of heirloom varietals. In many cases, farmers grow their own unique heirloom varietals, the majority of which grow else in the world (Boot, 2011). For example, among the several renowned types of coffee known with very fine quality coffee in Ethiopia Hararghe (former Harar), Sidama and Yirgachefe coffees are well known in contributing their own special quality coffee International Journal of Plant Breeding and Crop Science Vol. 4(2), pp. 187-196, April, 2017. © www.premierpublishers.org. ISSN: 2167-0449 Research Article
  • 2. Ameyu et al. 188 acclaimed for its aroma and flavor characteristics to the world (Boot, 2011; Emebet et al., 2013). Currently these coffee types are registered for trademark in 27 European countries, USA, India, Japan, Canada, Saudi Arabia, China and South Africa (Emebet et al., 2013). Thus, they are sold at a premium price both at domestic and international coffee markets (ITC, 2002). The region known as Hararghe comprises the eastern most of the coffee growing regions of Ethiopia. It is subdivided into east and west Hararghe zone (Boot, 2011). Coffee produced in this region is a Coffea arabica species growing in the highland and midland areas of the zones and well recognized specialty and exemplified category coffee which is grown mostly without shade (Desse, 2008). It is characterized by medium sized beans with greenish yellow color, medium acidity and full body and a distinctive mocha flavor. Quality of Hararghe coffees are notable for a fruity characteristic and have a distinct note of blueberry, though many other fruity and fruit-like aromatic flavors can occur. Furthermore, it can be differentiated by its amber or golden bean coffee (Boot, 2011). Internationally it is known and recognized as Harar trade brand name and highest premium coffee in the world (EMT, 2012). Therefore, this distinctive fine quality coffee type of this region is currently known by the name of specialty coffee in international market. Among the Hararghe (Harar) coffee, east Hararghe coffees tend to have the most distinct blueberry flavors as the result of a certain varietal growing in a certain soil type in East Hararghe. This is “naturally occurring” amber bean coffee, and often has an excellent, vibrant and rich blueberry flavor. However, often west Hararghe coffees have a thicker, smoother body, and a milder fruit flavor (Boot, 2011). Interms of production quantity west Hararghe zone is contributing about 65% of Hararghe coffees. As a foreign exchange earner Hararghe coffee is exported to all over the world, but there is a particular demand for it in Saudi Arabia. This constant demand and its premium quality tend to keep the price for commercial grade Hararghe coffee slightly higher than most other Ethiopian coffee regions (Boot, 2011). However, merely having such potential will not bring significant contribution to country’s income unless and otherwise the products meet the demand of customers in terms quality. This mainly requires the improvement of agronomic practices such as harvesting, processing and handling practices. Most farmers in the area harvest coffee by allowing more cherries to mature, immature, some to dry, some to fallen on the ground and some cherries are unripe on the mother tree. They do not practice the recommended selective picking. The main reason discourages farmers not to harvest and process their coffee with great care is absence of premium price for good quality harvest and drying coffees at primarily transaction center (Mohammedsani, 2014). Additionally, in Hararghe only natural sundried method has been used in which coffee cherries are harvested once from the tree and dried on plastic sheet or bare ground (Boot, 2011; Mohammedsani, 2014). These indicate that, the deterioration of physical bean quality of Hararghe coffee. Related to this Desse (2008) reported that, poor harvesting practices such as stripping and collecting dropped fruits from the ground and improper postharvest handling practices such as bad processing and drying on bare ground have contributed a significant share for the production of low quality of green coffee bean. Particularly, in Hararghe coffee raw bean quality is declining from time to time due to poor harvesting and postharvest processing practices (Mohammedsani, 2014). This adverse situation has been largely attributed to the unsatisfactory quality of the coffee beans rising from poor harvesting and processing methods (Mutua, 2000) attempted to gain some insight into the resolution of the problem. Thus, there is a strong interest for this region of coffee in producing and marketing coffee of higher quality to alleviate financial difficulties encountered by coffee farmers (Behailu et al., 2008). With these reasons, the first priority in research is on coffee quality, paying much attention to quality improvement and maintenance in the coffee growing regions. Hence, in order to produce and supply Hararghe coffee bean with its inherent quality characteristics, it is necessary to conduct research on this first research priority topic that have never been studied in the areas. Therefore, this study was initiated with the specific objective to evaluate the effects of harvesting and postharvest processing methods on raw quality attributes of a Hararghe coffee genotype. MATERIALS AND METHODS Description of the study area The experiment was conducted at Mechara Agricultural Research Center (MCARC) in 2013 cropping season on young established Hararghe coffee genotype. The center is located near Mechara town in Darolabu district of West Hararge Zone, eastern Ethiopia. It is 434 km east of Addis Ababa, the capital city and 115 km southeast of Chiro town, the zonal capital. Geographically, it is located between latitude of 40o 19’29" North and longitude of 8 o 36'38" East at an altitude of 1760 m.a.s.l. The soil of the center is sandy clay loam that is suitable for Arabica coffee production (McARC, 2015). The area receives an average annual rainfall of 1100 mm with high variability in the onset and cessation of the main rainfall season. Its annual mean minimum and maximum air temperatures are 14 o C and 26 o C, respectively. Experimental material One promising Hararghe coffee selection namely H- 622/98 was used from coffee variety verification research trial started in 2005. The selected genotype was previously collected from Gemmechis district (Chirkane
  • 3. Int. J. Plant Breeding and Crop Sci. 189 site) of west Hararghe zone in 1998 and planted at Mechara on station in July 2005. It is among the top yielding genotype from the 14 promising Hararghe coffee selections under evaluation. Regarding its bean quality characteristics, good flavor note is scored for this genotype which shows the typical quality profile of Hararghe coffee (Abdi et al., 2011). Experimental design The treatments were arranged using factorial arrangement in a completely randomized design with three replications. Accordingly, the treatments consists of two types of coffee harvesting methods viz.; strip harvesting and selective picking were combined with six types of postharvest processing methods viz.; dry processing drying on bare ground, dry processing drying on cemented ground, dry processing drying on plastic sheet ground, dry processing drying on mesh wire, semi-washed processing and wet processing drying on mesh wire. Experimental procedures Coffee harvesting Coffee cherries were harvested following both harvesting methods (strip harvesting and selective picking). In these methods, cherries were harvested at different times. This is because under strip harvesting, cherries were harvested once at a time when ripe red, turned red, immature green and turned dried cherries (when 75% of the cherries were reached at full ripe) whereas selective picking required more than one harvesting since only red ripe cherries were harvested as they attained this stage. For this, fruit bearing coffee trees were assigned for the two harvesting treatments in such a way that the odd and even number coffee trees were used for strip and selective harvesting methods, respectively. Consequently, harvesting of the coffee cherries was done from October to December 2013. Finally, all cherries harvested by each harvesting method was passed to different postharvest processing methods. Dry processing All cherries harvested using both harvesting methods were immediately spread out to dry in the sun using four drying methods (bare, cemented and plastic sheet ground and raised mesh wire table). They were stirred regularly to promote even drying, prevent fermentation and the development of mold in each treatment. All sample cherries were dried up to when their outer shell skin became dark brown and brittle (Clark, 1985; Boot, 2011) or when their approximate moisture content attained 11.5%. Finally dried coffee cherries were collected and hulled with mortar as farmers are practicing carefully and cleaned (ISO, 2004). Wet processing Under this processing method the cherries were pulped using single disc hand pulper separately as per the harvesting method. The pulped cherries were collected inside the large size plastic buckets where pulps and floater parchments bean were removed. Subsequently, the wet parchment beans were transferred into other bucket and fresh water was added on it until parchment beans were totally submerged inside the water for fermentation. They were fermented for 40 hours (Woelore, 1993) during which the water was changed three times. When the mucilage was totally degraded, parchment coffee beans were washed intensively for the total removal of mucilage (Boot, 2011). The resulting green parchment beans were prepared and allowed to dry under full sun condition on 80cm above ground raised mesh wire table (Behailu et al., 2008) until approximately their moisture content attained 11.5%. Finally, the samples were slowly hulled and hand polished to remove the parchment and silver skins from green coffee beans (ISO, 2004). Semi-washed processing Similar to wet processing method, the harvested cherries were pulped with single disc hand pulper and collected in large size plastic buckets. However, as opposed to it, parchment beans were immediately washed manually and rubbed with canavas cloth by hand until the mucilage was clearly removed from the parchment beans then after the parchment beans were transferred to above ground mesh wire drying table under full sun conditions (ES, 2001). Finally, similar to wet processing method the parchment beans were prepared for raw quality evaluation. Laboratory analysis All samples prepared by using the above harvesting and processing methods were assigned an arbitrary code (an identity letter and number) and brought to coffee quality laboratory of Jimma Agricultural Research Center where the green coffee beans were evaluated for different raw quality attributes. The moisture content of the sample was checked using Electronic Rapid Moisture Tester (HE 50, Germany) to make the uniform required moisture level of all samples. Bean defect collection Currently, Ethiopian Commodity Exchange (ECX) evaluated raw coffee bean quality with bean defect (primary and secondary defect), odor, color, shape and make as raw quality attributes of green bean in Ethiopia (ECX, 2010). Accordingly, 350 g of green coffee bean sample from each treatment combination was used for defect green bean quality evaluation. Thus, as per ECX (2010) evaluation standard, green coffee beans were evaluated for bean defect by counting primary defect and weighting secondary defects. Defects like full black, full sour, fungus attacked, foreign matter and insect damaged beans were considered as primary defects whereas partial black, partial sour, floater, immature, withered, shell, slightly insect damaged, foxy, under dried, over dried,
  • 4. Ameyu et al. 190 mixed dried, stinkers, faded, coated, light and starved beans were identified as type of secondary defects. Data collection Bean size: it is the size of coffee beans which was determined by the conventional screen analysis in which the weight fractions retained on sieve of screen 14 with respective hole diameter of 5.55 mm were recorded and percentage was calculated and used for data analysis. Bean weight: hundred (100) beans were randomly counted from each 350 g sample and their weight was measured in gram using a two digit sensitive balance. Primary defect (counted): different types of coffee bean defects recognized as full black, full sour, fungus attacked, foreign matter and insect damaged were counted from 350 g of green beans sample and the percentage of total defect number was calculated and scored out of 15% for unwashed and 10% for washed coffee bean Secondary defect (weighted): other bean defect type including partial black, partial sour, floater, immature, withered, shell, slightly insect damaged, foxy, under dried, over dried, mixed dried, stinkers, faded, coated, light and starved were collected together from 350 g of green beans and weighed by using a sensitive balance. The percentage of total defect weight was calculated and scored out of 15% for unwashed and 10% for washed coffee bean. Shape and make: it is the structural make up of different kinds of beans. It was evaluated out of score 10% only for washed coffee as very good, good, fairly good, average, fair and small and weighed accordingly (ECX, 2010). Color: it is the overall physical appearance of coffee beans that was evaluated out of score 5% only for washed coffee as bluish, grayish, greenish, coated, faded and white (ECX, 2010) and weighed accordingly. Odor: it is the olfaction of coffee beans which was evaluated out of score 10% for unwashed and 5% for washed coffee as clean, fairly clean, trace, light, moderate and strong (ECX, 2010). Data analysis Data were subjected to analysis of variance (two way ANOVA) using general linear model (GLM) procedure of Statistical Software Program (SAS 9.2). Whenever ANOVA showed significant variation, LSD at 5% probability level was used for treatment mean separation. RESULTS AND DISCUSSION Bean size Coffee harvesting methods resulted significant (P≤0.01) variation on bean size, while the effect of postharvest processing methods had no significant effect on bean size. This shows processing did not change the size of the bean since it processes bean as it is. However, the interaction effect of harvesting methods by postharvest processing methods showed significant (P≤0.05) variation on bean size. The interaction effect of coffee harvesting and postharvest processing methods on total percentage of bean size retained above screen size 14 ranged from 84% to 93% for selective harvesting and the dry processed dried on cemented ground and semi-washed processed dried on mesh wire, respectively (Table 1). This depicted that coffee beans harvested by selective picking and treated with different postharvest processing methods can meet export standards. In line with this the finding by Mekonen (2009) indicated that, selective harvested coffee of different varieties showed significant variation in bean size by recording highest percentage of beans retained above screen. However, postharvest processing methods combined with strip harvesting ranged from 69 to 76% for washed and unwashed coffee dried on mesh wire and bare ground, respectively. These can fall under category of rejected commercial coffee according to ECX (2010) standard, in which any Ethiopian export coffee shall have the minimum 85% of bean weight remaining on top of screen 14. This could be due to the effect of collecting different type of fruits viz; immature, mature, red, dried, small, and big size beans. These all together might produce the size of the bean with less uniformity and low percentage of beans below the required screen size. Similarly, Anteneh (2011) reported that, poor harvesting practices, such as stripping different type of fruits together, reduces quality and increases losses by promoting uneven distribution of bean size. In contrast, selective harvesting of red fruits produced uniform bean size that is above the minimum required bean screen size. This in agreement with the finding of Bertrand et al. (2006), who suggested that for C. arabica, picking of red cherries at appropriate maturity stage, gives the best coffee bean size. Similarly, ITC (2002) reported that the quality of a bean size comes from care taken during harvesting. In general, the percentage of green bean size decreased from selective harvesting (93%) to strip harvesting (69%) combined with semi-washed processing and drying on raised mesh wire table, respectively (Table 1). This is because in contrast to selective harvesting, for strip harvesting there was no any sorting practice rather the beans were directly taken to processing that could increase the number of under sized bean coming from striping of immature and defective cherries. Hence, sorting under size beans can increase percentage of beans retained above screen size 14, though it incurs additional costs. Currently, coffee traders often practice this before transporting it to central market for quality assurance and grading in order to improve quality of coffee and economic return from export market. This support with result of Anteneh (2011) who reported that in order to improve
  • 5. Int. J. Plant Breeding and Crop Sci. 191 Table 1. Interaction effect of coffee harvesting and postharvest processing methods on bean size above screen size of 14 at Mechara Harvesting Methods Postharvest Processing Methods % of bean screen size Strip harvesting Dry processed dried on bare ground 76.00 c Dry processed dried on cemented ground 73.83 cd Dry processed dried on plastic sheet ground 74.70 cd Dry processed dried on mesh wire 73.77 cd Semi-washed processed dried on mesh wire 69.20 d Wet processed dried on mesh wire 69.83 cd Selective harvesting Dry processed dried on bare ground 87.90 ab Dry processed dried on cemented ground 84.33 b Dry processed dried on plastic sheet ground 89.00 ab Dry processed dried on mesh wire 85.87 b Semi-washed processed dried on mesh wire 93.70 a Wet processed dried on mesh wire 90.53 ab LSD (5%) 6.36 C.V (%) 4.70 Mean 80.72 Mean values followed by the same letter (s) within a column are not significantly different at P≤0.05 level of significance. quality of Hararghe coffee, traders practice some value adding activities like removing defective and under sized beans through sorting and cleaning. From the present finding, the uniform, bold and heavy coffee beans were obtained from selective harvesting coffee treated under different postharvest processing methods. Besides, this result perhaps attributable due to the genetic characteristics of Hararghe coffee that known by medium bean size that have acceptability in history of Ethiopian coffee market and also good growth habit of this genotype may contribute to the present finding in production of medium to large bean size. This would improve final raw and cup quality of the beans and mostly preferred by roasters to pay premium price. According to Agwanda et al. (2003), bean physical characteristics such as bean size are unified criteria for conducting coffee business within the international market. Similarly, bold and medium bean size has a particular importance to roasters, as uniform bean size would produce uniform roast (Yigzaw, 2005; EAFCA, 2008). Additionally, Barel and Jacquet (1994) supported that, roasting uneven sized beans the smallest tend to burn and the largest tend to be under-roasted, this tend to reduce final quality of the brew. Bean weight The results indicated highly significant variation in 100 bean weight due to the main effect of harvesting and postharvest processing methods. But the interactions did not significantly affect 100-bean weight. As a result, higher value was recorded from selective harvesting method compared to strip harvesting (Table 2). This might be due to collection of different stage and sized fruits together at once during harvesting which not only affect coffee bean size but also bean weight of the coffee. ITC (2011) also reported that, picking of immature cherry with mature cherries could cause the reduction of weight of the beans. In conformity with this Boot (2006) reported that under almost all conditions, the specific weight of a ripe cherry is greater than that of an immature cherry, it is heavier, weighing up to 20% more. Similarly, processing methods highly differed and produced an average of 100-bean weight ranging from 12.43 g to 14.97 g. Dry processed and dried on different methods showed light to medium beans and wet processed had medium to heavy beans with mean value of 14.97 g per 100-beans (Table 2). This result generally revealed that dry processed coffee with different postharvest practice produced bean weight lighter than that of wet processed coffee. This is because under dry processed coffee all harvested cherries (mature and immature) were directly spread to full sun without sorting and clearing as opposed to washed processed in which sorting of abnormal, immature and floaters were performed. In agreement with this, Mekonen (2009) found out that significant difference in bean weight due to effect of processing methods in which dry processed coffee had lower weight than wet processed because of the removal of immature and immersed fruits in washed processing. Primary defect The effect of harvesting and postharvest processing methods was highly significant variation on primary defect in unwashed or dry processed coffee. Likewise, harvesting methods significantly (P≤0.01) influenced the primary
  • 6. Ameyu et al. 192 Table 2. Main effect of coffee harvesting and postharvest processing methods on 100-bean weight of promising Hararghe coffee genotype at Mechara Treatments 100-Bean Weight (g) Harvesting Methods Strip harvesting 13.24 b Selective harvesting 14.97 a LSD (5%) 0.34 Postharvest Processing Methods Dry processed dried on bare ground 12.43 d Dry processed dried on cemented ground 13.48 c Dry processed dried on plastic sheet ground 14.40 b Dry processed dried on mesh wire 14.57 ab Semi-washed processed dried on mesh wire 14.78 ab Wet processed dried on mesh wire 14.97 a LSD (5%) 0.59 C.V (%) 3.50 Mean 14.11 Mean values followed by the same letter (s) within a column are not significantly different at P≤0.05 level of significance Table 3. Raw quality attributes of natural sundried coffee as affected by main effect of harvesting and dry processing methods Treatments Raw quality parameter PD (%) SD (%) Odor (%) Harvesting methods Strip harvesting 3.25 b 6.62 b 8.56 b Selective harvesting 14.00 a 12.75 a 9.06 a LSD (5%) 1.78 1.82 0.49 Postharvest processing methods Dry processed dried on bare ground 6.25 b 6.75 b 8.42 Dry processed dried on cemented ground 8.50 b 9.50 a 8.67 Dry processed dried on plastic sheet ground 8.50 b 10.50 a 9.08 Dry processed dried on mesh wire 11.25 a 12.00 a 9.08 LSD (5%) 2.51 2.57 NS CV (%) 23.80 21.70 6.50 Mean 8.62 9.69 8.81 Mean values followed by the same letter within columns are not significantly different at P≤0.05 level of significance; PD=Primary defect; SD=Secondary defect; NS= Non-significant defect, but postharvest processing methods did not influence in washed coffee processing. Similarly, the combined effect of harvesting and processing methods did not significantly influence primary defect in both washed and unwashed coffee. The lowest average numbers of primary defects were counted with mean value of 14% out of 15% in unwashed coffee (Table 3) and 9.67% out of 10% in washed coffee (Table 4) for selective harvesting. In contrast, beans from strip-harvested coffee recorded the highest number of primary defect with mean value of 3.25% in unwashed coffee (Table 3) and 6.67% in washed coffee (Table 4). From each treatment, only few numbers of defects like full black, fungus attacked and insect damage were recorded as primary defects in selective harvesting, indicating reduced problems of coffee harvested with selective red ripe cherries gave beans free from primary defect in both processing methods. Similarly, Elsa et al. (2015) found that the probability of coffee varieties free from any primary defect with mean value of 15% was due to proper harvesting of red ripe cherries followed by proper postharvest handling. Moreover, the result revealed that strip harvesting produced coffee beans with high number
  • 7. Int. J. Plant Breeding and Crop Sci. 193 Table 4. Raw coffee quality attributes of washed coffee as affected by main effect of coffee harvesting and postharvest processing methods Harvesting Methods Raw quality attributes PD (%) SD (%) Odor (%) SM (%) Color (%) Strip harvesting 6.67 b 3.67 b 4.29 b 6.05 b 3.17 b Selective harvesting 9.67 a 9.33 a 4.88 a 8.14 a 4.18 a LSD (5%) 1.72 1.54 0.21 0.43 0.24 Postharvest processing methods Semi-washed processed dried on mesh wire 8.33 6.00 4.56 7.16 3.65 Wet processed dried on mesh wire 8.00 7.00 4.61 7.03 3.70 LSD (5%) NS NS NS NS NS C.V (%) 15.80 17.80 4.20 4.50 3.90 Mean 8.17 6.5 3.67 7.10 4.58 Mean values followed by the same letter within columns are not significantly different at P≤0.05 level of significance; NS=Non-significant; PD=Primary defect; SD=Secondary defect; SM=Shape and make of defects which may be due to harvesting of insect and fungus damaged fruits. This was in agreement with the finding of Barel and Jacquet (1994), who reported traditional selective hand pricking as opposed to strip harvest, produced the best quality green coffee by decreasing the percentage of defects in coffee batches. With regard to effect of dry processing on primary defect, the highest (11.25%) and the lowest (6.25%) mean values were produced for cherries dried on mesh wire and bare ground, respectively. However, coffee cherries dried on plastic sheet and cemented ground had the same value of 6.50% (Table 3). This might be due to the fact that, fruits dried on bare, plastic sheet and cemented floor had contact with foreign matter, exposed to re-wetting of cherries and less air movement during drying than the raised mesh wire. This could favor development of mold as well as black bean formation. This in turn might have increased the number of primary defect counts that can affect final physical appearance of the beans. The results support Enyan et al. (2013) who pointed out that the drying of coffee beans on the concrete floor and black polythene sheet on concrete floor resulted in a higher percentage of defective beans than on the raised raffia plastic sheet. The authors additionally reported that insect damage and black beans constituted more than 70% of the defective beans, related to the processing methods used. This is in line with Berhanu et al. (2014) who reported that, inappropriate postharvest management practices increase the foxy bean formation that maximizes the degree of defect counts and affects the odor and color of the coffee that finally affects the raw quality of green beans. Similarly, the present study showed that the presence of defects in coffee was indicative of its reduced quality that translated into price and acceptability of the coffee on the commodity market. Negussie et al. (2009) also pointed out that properly processed coffee is free of off-flavor with few defective beans. Secondary defect The result from analysis of variance showed that, there was a highly significant variation in secondary defects due to main effect of harvesting and postharvest processing methods for unwashed coffee. Similarly, the effect of harvesting methods was significant (P≤0.01) but not for postharvest processing methods that showed non- significant variation in washed coffee. However, the interaction effect of harvesting and postharvest processing methods did not significantly affect secondary defect in both dry and washed coffees. Selective harvesting had high mean value of 12.75% indicating relatively pure coffee bean. However, the lower mean value (3.23%) was recorded from strip harvesting, which indicates high number of secondary defect in poorly harvested and unwashed coffee (Table 3). Similarly, in washed coffee, the highest mean value (9.33%) of secondary defect was recorded for selective harvesting and the lowest (3.33%) for strip harvesting (Table 4). These showed that selective harvesting produced coffee beans free from secondary defects as compared to strip harvesting in dry and wet processed coffee. This is because selective harvesting involves only picking of the red, fully ripe and normal cherries carefully from tree, while in strip harvesting collecting of entire crop (immature, mature, ripe, over mature, dried, insect damaged) just by one pass through cropping season. This result is in line with Hicks (2002) who described that although selective picking is more expensive, but it can produce the best results of coffee by reducing number of defects thereby increase overall quality of coffee that are competent in the world market. Moreover, coffee processed and dried with different methods showed significant variation on secondary defects. Hence, the highest mean value (12%) was recorded for dry processed coffee and dried on mesh wire, while the lowest value (6.75%) of secondary defect was
  • 8. Ameyu et al. 194 recorded in dry processed and dried on bare ground followed by dried on cemented and plastic sheet ground (Table 3). The present finding agreed with Olamcam (2008) result where coffee that is well harvested and properly processed has no or very few broken beans and free of foreign matter (stick, stones, and leaves). Hicks (2002) identified that coffee that has been inappropriately dried would become brittle and produce too many broken beans that considered as secondary defect during hulling. Among the secondary defect observed in this study, the major ones are partial black, foxy, immature, and slightly insect damaged beans, coated, broken, shell and faded beans that have quality deteriorating effects. Odor The analysis of variance for unwashed coffee revealed significant variation (P≤0.05) on odor due to the main effect of coffee harvesting and did not affected by postharvest processing methods. In washed coffee, this was highly significant for harvesting methods, but did not differ due to postharvest processing methods. However, in both unwashed and washed coffee, the interaction effect showed non-significant variations on odor. In both harvesting methods, comparable higher mean values of odor (>8%) were recorded. However, for selective harvesting the mean values of odor was higher than strip harvesting in unwashed coffee (Table 3). All beans had odor point ranging from 8 to 9% which can be categorized as fair clean to clean (ECX, 2010). These showed that green coffee smell was affected due to improper harvesting method. This is in line with the finding of Olamcam (2008) who explained that, properly harvested and processed beans are free of unpleasant (bad) smells. In accordance with this result, Endale (2008) reported that, coffee with better management in each stage starting from harvesting until cupping turn out to have better odor. On the other hand, in washed coffee higher mean value above four of odor was recorded for selective harvesting method, which can be categorized under clean odor (Table 4). In general, unwashed coffee had fair clean odor and washed coffee had clean odor. Likewise, Anwar (2010) reported that wet processing methods resulted in high mean values (9.04%) as compared to dry processing methods (7.48%) for odor. Shape and make Coffee beans significantly (P≤0.01) varied for shape and make due to main effect of harvesting methods, but not significantly affected by postharvest processing methods and the interaction. The highest average value of 8.14% was recorded for selective harvested coffees which possessed a very good shape and make with more uniform appearance (Table 4). Correspondingly, Mekonen (2009) reported the selectively picked red fruits of different coffee accessions have very good shape and make under both wet and dry processing. In contrast, the lower shape and make (6.04%) was recorded from strip-harvested coffees indicating fairly good shape and make with small bean size (Table 4).This perhaps is due to the mixing of mature, immature, under sized and over fermented (on mother tree dried) cherries during strip harvesting that could produce poor appearance beans thereby reduce the flavor and acidity of coffee. In connection with this Anteneh (2011) reported that, either mixing defective beans during harvesting by producers was the main reasons for deteriorating coffee physical appearance. However, Wintgens (2004) indicated that the shape and structure of beans are the result of both genotype and environmental factors. Color Green coffee bean color was significantly (P≤0.01) influenced by harvesting methods, however, both postharvest processing and interaction effects of harvesting and postharvest processing did not influence bean color of washed and semi-washed coffee. The result was as opposed to the result of Mekonen (2009) who reported high significant variations among coffees processed in different processing methods. This may be due to the unique genetic characteristics of a Hararghe coffee genotype that can maintain its bean color in any processing method. Thus, higher mean value (4.10%) was recorded for selective harvested coffees as compared to striped coffee beans (Table 4). This might be due to the mixture of harvesting of different fruits together at once. This agreed with the report of ITC (2002) in that uneven color of the beans revealed from mixing of immature, overripe and ripe coffee cherries during harvesting. Anwar (2010) also found that if coffee is harvested before the beans are ripe or at immature stage, the end product will show color defect. Similarly, Olamcam (2008) reported that, well harvested and processed coffee had clean and uniform color. Furthermore, uneven bean color can be from poor processing, incorrect moisture content during the fermentation, premature aging of the coffee, poor drying techniques, harvesting of overripe cherries, keeping cherries a long time before pulping and/or insufficient washing after natural fermentation (ITC, 2002). CONCLUSION Improper harvesting and postharvest processing are adversely affecting quality of the coffee produced in Hararghe region. Taking these problems into account, this study was conducted to evaluate the effect of harvesting and postharvest processing methods on raw quality of a Hararghe coffee genotype. For the study, the coffee cherry samples were prepared from one young established Hararghe coffee selection planted at the Mechara. The evaluation for raw appearance of green bean was conducted in the coffee quality laboratory. Hence, the finding indicated that, bean size was
  • 9. Int. J. Plant Breeding and Crop Sci. 195 significantly affected by harvesting and the interaction of harvesting and postharvest processing methods while, bean weight was affected highly by both harvesting and postharvest processing methods. Coffee beans harvested by selective harvesting and treated under different postharvest processing methods had 85% and above the minimum required bean size for export coffee as compared to strip harvested beans in which all beans are recorded under rejected coffee due to a number of small beans (<76%). Similarly, weight of 100-beans was higher for coffee samples harvested by selective harvesting as compared to strip harvesting. Primary and secondary defects and odor of both unwashed and washed coffee as well as color, shape and make of washed coffees were highly significantly influenced by harvesting methods. Selective harvesting produced beans with high raw quality of all attributes in both washed and unwashed coffee. Postharvest processing treatment highly significantly influenced all raw quality attributes except odor of unwashed coffee but these were not significantly affected in washed coffees. Natural sundried coffee on raised mesh wire was better in producing high raw bean quality with the lowest primary and secondary defects followed by coffee dried on plastic sheet ground floor. In general, the overall findings of the present study indicated that strip harvesting as practiced by many Hararghe coffee producers has a determinant effect on raw and physical appearance of coffee quality in the Hararghe areas. This showed that if producers avoid stripping cherries and practiced selective picking of red cherries only, the quality of coffee could be improved. Moreover, drying coffee on bare ground highly reduced raw, abnormal color and unpleasant odor. Therefore, proper harvesting and drying practices were found to be crucial in maintaining the typical inherent quality characteristics of Hararghe coffee beans. ACKNOWLEDGEMENTS The authors would like to thank the Oromia Agricultural Research Institute (OARI) for sponsoring this research work and Mechara Agricultural Research Center (McARC) for facilitating the wok on the field at Mechara main research station. Further, we wish to convey our deepest thanks to all the McARC’s coffee research case team staff, namely, Ahmed Aliyi, Atika Eliyas, Sorse Beyissa, Hika Bersisa, Mulugeta Mamo and Assefa Juhar for their technical support as a group during filed sample preparation. Lastly but not the least we would also like to acknowledge all the Jimma Agricultural Research Center coffee processing and quality research staff namely, Mr. Abrar Sualeh, Nigussie Mekonen, Mikru Tesfa, Wabi Bajo, Dasse Baley and Murad Abraya and Miss Saida Abdu and Foziya Kemal for their willingness to host and sharing their experiences, supporting and facilitating data collection in the laboratory. REFERENCES Abdi A, Chala B, Mohammedsani A (2011). Verification of promising Hararghe coffee selections. A research paper presented on Annual Completed Research Activities Review of Oromia Agricultural Research Institute at Zuway Fishery Research Center, April 14-17, 2011, Batu, Ethiopia. Agwanda CO, Baradat P, Eskes AB, Cilas C, Charrier A (2003). Selection for bean and liquor qualities within related hybrids of Arabica coffee in multi local field trials. Euphytica, 131: 1-14 Alemseged A, Yeabsira Z (2014). Coffee export business in Ethiopia: Business start-up and operational manual. 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