by prachee rajput, Barkatullah University, Bhopal (INDIA)

1. BIODIVERSITY OF ENDOPARASITIC HELMINTHS
IN FRESH WATER TELEOSTEON FISHES
Submitted by
PRACHEE RAJPUT
Student of M.Sc sem –II
DEPT OF ZOOLOGY AND APPLIED
AQUACULTURE
B.U BHOPAL

2. SYNOPSIS
INTRODUCTION
OBJECTIVE
MATERIAL AND METHOD
RESULT
CONCLUSION
REFERENCE

3. INTRODUCTION
Biodiversity is the degree of variation of life. It a
measure of the variety of organisms present in
different ecosystems. This can measure to genetic
variation ecosystem variation on species variation no
of species with in are biome and planet.
Parasites that lives off or in another
organism obtaining nourishment and protection while
offering no benefit in return.

4. OBJECTIVE
To know the THE BIODIVERSITY OF HELMINTHS
PARASITES among fresh water fishes.
To know there prevalence and intensity
abudance among fresh water fishes.

5. Material &method
• The host fishes (Channa striatus, Xenentadon
cancila, Nandusnandus, Mystus,
Glossogobius, Channa marulius and Ompok
Channa punctatus Channa marulius ) were
collected from the local fish markets of Bhopal
regions like Habibgang, Piplani, govindpura,
Barkhera, Itwara, was collected thrice in a
week.

6. 1. Parasites were collected as ecto-parasites and endo-parasites from
skin, gills and internal organs by dissecting the fish, respectively in
normal saline and were examined alive condition in order to identify
them.
2. Parasites collected were fixed in fixative - alcohol formal acetic (AFA)
and glycerin alcohol in cuticular parasites.
3. The fixative should be thoroughly removed from specimens prior to
sustaining wash out formalin in distilled water and AFA in 70% alcohol.
4. The specimens preserved in 70-90% alcohol were dehydrated
through the alcoholic series 30% (10mins), 50% (10 mins).
5. Then they were kept 5-10 minute in dilute Aceto Carmine for
staining.
6. Specimens were slightly washed within 70% alcohol stain.
7. And further dehydration by passing specimens through an ascending
series of alcohols i.e. 70% (10 mins.), 90% (10 mins.) and 100% alcohol
(10 mins).

7. •After complete dehydration, the specimens were transferred to a
cleaning agent (Xylene) which rendered the specimens transparent
mount in DPX.
•The stains, dehydrated and cleared specimens were mounted in Dextrin
Plasticized Xylene (DPX) mountant.
•The cover glass was preferably lowered horizontally to avoid trapping
of air bubbles and displacement of specimens.
Finally photographs were taken with the help of Olympus Microscope at
different combinations of magnification.
5. Statistical analysis
(i) Prevalence = Total No. of Hosts Infected ×100
Total No. of Hosts Examined
(ii) Mean Intensity = Total No. of parasites
Total No. of Infected Hosts Examined
(iii) Relative Abundance = Total No. Parasites
Total No. of Hosts Examined

8. Fig; 1 Channa striatus Fig; 2 Glossogobius giuris

9. Fig;3 Mystus seenghala Fig; 4 Channa punctatus

10. Fig; 5 Ompok Fig; 6 Nandus nandus

11. Fig. 6. Xenentadon cancila
atr
;
Fig. 7.
Clariasbataracus

12. Acanthocephala in Channa Striatus

13. RESULT
• During present investigation freshwater fishes collected
from the local fish markets were survey for the diversity of
helminth parasites. Numbers of host fishes were collected
twice in a week (Figs.1,2, 3, 4, 5, 6, 7, 8, 9).
• 1. Altogether 204 fishes, belonging to 7 families and 9
species were examined. 114 fishes were found positive
against helminthes infection. Total prevalence of helminth
parasites was calculated to be 47.50%.
• 2. The incidence of infection with helminths varied in
different fish families, being lowest (15.38%) in Clariidae
and highest (85.71%) in Ophiocephalidae.

14. While the month-wise intensity and abundance among
fish host species was showed in graph
0
10
20
30
40
50
60
70
80
90
100
Oct-14 Nov.14 Dec.14
Prevalence%
Bagridae
Siluridae
Belonidae
Nandidae
Ophiocephal
idae

15. CONCLUSION
• The biodiversity of Acanthocephalian is more
found in the teleosteon fish in month of oct
to dec . Some parasites are found in larvae
and some in adult stages.