Bacteria growth Explain a growth curve. Explain lag phase, exponential growth and stationary phase. What are gene products? What is the role of DNA polymerase? What is a promoter and a terminator? In which molecule are they active? What is the role of RNA polymerase? What is a ribosome binding site? In which molecule is it active? What is an open reading frame? Solution Growth curve: A graphical representation of how a particular phase moving over time. Growth curves are classifed in to three different types, those are linear, exponential or cubic. lag phase: The period where the individual bacteria are maturing and not yet able to divide. During the lag phase of the bacterial growth cycle, synthesis of RNA, enzymes and other molecules occurs. exponential growth: This phase (sometimes called the logarithmic phase ) is a period characterized by cell doubling. stationary phase: As the bacterial population continues to grow, all the nutrients in the growth medium are used up by the microorganism for their rapid multiplication. What are gene products? A gene product is the biochemical material, either RNA or protein, resulting from expression of a gene. What is the role of DNA polymerase? Enzymes that synthesize DNA molecules from deoxyribonucleotide What is a promoter and a terminator? Promoter - Region of DNA that initiates transcription of a gene Terminator - Region of DNA that ends transcription of a gene In which molecule are they active? DNA What is the role of RNA polymerase? Enzymes that synthesize RNA molecules from ribonucleotides What is a ribosome binding site? Shine Dalgarno sequence in mRNA is called ribiosome binding site In which molecule is it active? mRNA What is an open reading frame? Sequence of DNA that starts with start codon “ATG” (not always) and ends with any of the three termination codons.

1. Bacteria growth
Explain a growth curve.
Explain lag phase, exponential growth and stationary phase.
What are gene products?
What is the role of DNA polymerase?
What is a promoter and a terminator?
In which molecule are they active?
What is the role of RNA polymerase?
What is a ribosome binding site?
In which molecule is it active?
What is an open reading frame?
Solution
Growth curve: A graphical representation of how a particular phase moving over time. Growth
curves are classifed in to three different types, those are linear, exponential or cubic.
lag phase: The period where the individual bacteria are maturing and not yet able to divide.
During the lag phase of the bacterial growth cycle, synthesis of RNA, enzymes and other
molecules occurs.
exponential growth: This phase (sometimes called the logarithmic phase ) is a period
characterized by cell doubling.
stationary phase: As the bacterial population continues to grow, all the nutrients in the growth
medium are used up by the microorganism for their rapid multiplication.
What are gene products?
A gene product is the biochemical material, either RNA or protein, resulting from expression of
a gene.
What is the role of DNA polymerase?
Enzymes that synthesize DNA molecules from deoxyribonucleotide
What is a promoter and a terminator?
Promoter - Region of DNA that initiates transcription of a gene
Terminator - Region of DNA that ends transcription of a gene
In which molecule are they active?
DNA
What is the role of RNA polymerase?
Enzymes that synthesize RNA molecules from ribonucleotides

2. What is a ribosome binding site?
Shine Dalgarno sequence in mRNA is called ribiosome binding site
In which molecule is it active?
mRNA
What is an open reading frame?
Sequence of DNA that starts with start codon “ATG” (not always) and ends with any of the
three termination codons