Codon optimization is one of the key steps in achieving the high-level expression of target gene. There are some key factors for consideration including transcription efficiency, translation efficiency, gene synthesis and protein folding. Creative Biogene can optimize almost perfectly these key factors in codon optimization.

1. Codon Optimization Technology
Tel: 1-631-626-9181 Fax: 1-631-614-7828
Email: info@creative-biogene.com
Website: http://www.creative-biogene.com

2. Codon optimization is one of the key steps in achieving the high-level expression of
target gene. There are some key factors for consideration including transcription
efficiency, translation efficiency, gene synthesis and protein folding. Creative Biogene can
optimize almost perfectly these key factors in codon optimization.

3. Some necessary consideration factors in codon optimization

4. mRNA
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5. tRNA abundance
Owing to different tRNAs identify several synonymous codons and the
content of these synonymous tRNAs are different, therefore, the efficiency of
translation also depends on the number of tRNA. Preferred codons are those that
can base pair optimally with the most abundant tRNA. Generally this involves
Watson - Crick pairing or, when bases are modified in the tRNA, some
modifications in optimal binding occur.
Codon usage bias
There are obvious biases of synonymous codon in bacteria,
Escherichia coli, yeast and some expression systems of higher biological.
This can directly affect the efficiency of translation. So when one gene is
expressed in a heterologous system, the codon usage bias should be taken
into account.

6. Complete mRNA structure
Next to 5'UTR and 3'UTR, The complete mRNA structure also has significant effect on
the process of protein translation. Recently, from the systematic study of major factors
which affect the protein expression, we know that the folding of mRNA, especially the
mRNA structure near the RBS, plays an important role in protein expression. For example,
the level of protein expression can be greatly improved by reducing the secondary
structure near the start codon.

7. The theory of gene design
Codon adaptation index (CAI)
CAI measures the proximity between the codon which is used by one gene
and the codon which is used in gene with high expression. CAI is between
0 and 1. The closer that the CAI is to 1, the higher level of gene expression
is. In general, CAI>0.8 means that gene expression has a high level.
Codon correlation index (CCI)
Researches show that codon correlation of specific host exert considerable
influence on the expression of heterologous genes. Codon correlation, such
as codon usage, double codon usage, dynamic of translation phase and GC
content, describe an integrated evolutionary environment characteristic of
species codon selection.

9. Creative Biogene offers gene synthesis and cloning services for your
scientific research as follows:
 Codon optimization for any given expression system (upon request);
 Synthesis genes of interest with or without tags;
 Cloning the target sequence into a standard vector or a vector of your choice;
 Sequencing the final construct to confirm 100% matching your requested sequence;
 Delivery of purified plasmid DNA construct and its bacterial stock.

10. Thank You!
Tel: 1-631-626-9181 Fax: 1-631-614-7828
Email: info@creative-biogene.com
Website: http://www.creative-biogene.com