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Living Environment
Part D (Required Labs)
Review
Beaks of Finches
 14

Species of Darwin’s finches

– Beaks vary in size and shape
– Bodies vary in size and shape
Beaks of Finches
 Different

beak sizes and shapes
differ in their efficiency at performing
particular tasks.
Beaks of Finches
 Why

do offspring of better-adapted
individuals inherit many of their
parents’ favorable variations?
Beaks of Finches
 Why

do offspring of better-adapted
individuals inherit many of their
parents’ favorable variations?
– Favorable variations allow parents
to survive and then reproduce more.
Their offspring then may possess
those variations.
Beaks of Finches
 What

are the parts of Darwin’s
theory of Natural Selection?
Beaks of Finches


What are the parts of Darwin’s theory of
Natural Selection?
–OVCSR
Only (Overproduction)
Vince (Variation)
Can (Competition)
Suck (Survival of the Fittest)
Rutabegas (Reproduction)
Beaks of Finches
 Variation



means ______________.

Why do the finches of the Galapagos
Islands show so much variation?
Beaks of Finches
 Variation



means _differences___.

Why do the finches of the Galapagos
Islands show so much variation?
– Each finch species adapted to their
different environments.
Beaks of Finches
 What

did the tools represent?
Beaks of Finches
 What

did the tools represent?

– The different beak shapes.
Beaks of Finches
 What

did the different seeds
represent?
Beaks of Finches
 What

did the different seeds
represent?
 The

different types of food available on
the island.
Beaks of Finches
 Why

are the 4 trials completed and
then averaged for each of the
“feedings”?
Beaks of Finches
 Why

are the 4 trials completed and
then averaged for each of the
“feedings”?
The

data is more accurate
when the trials are
averaged.
Beaks of Finches
 All

of the finches have similarities.
Why is this the case?
Beaks of Finches
 All

of the finches have similarities.
Why is this the case?
– All of the finches share a common
ancestor.
Relationships and Biodiversity
 Botana

curus (fictional plant for lab
purposes) produces Curol.
– This was supposedly used to
________________.
Relationships and Biodiversity
 Botana

curus (fictional plant for lab
purposes) produces Curol.
– This was supposedly used to cure
cancer.
Relationships and Biodiversity
The plants used in the lab, and what
they were modeled after… maybe?

This is the Pacific Yew.
It contains “Taxol” –
used to treat cancer.

This is what was
used in the lab,
though.
Relationships and Biodiversity
 Why

was it necessary to find an
alternative to Botana Curus?

 Story

of Taxol
Relationships and Biodiversity
 Why

was it necessary to find an
alternative to Botana Curus?
http://www.phcog.org/Taxus/Taxus_Web.html
 In

the lab, the plant was endangered
and was in short supply.

 (In

reality, the Pacific Yew only
produces one dose of drug per tree!
Scientists wanted to find an
alternative!)
Relationships and Biodiversity
 Chromatography:

Sorts
molecules
based on ____
Relationships and Biodiversity
 Chromatography:

Sorts
molecules
based on SIZE.
The smaller molecules
move faster up the
paper. Larger ones move
slower and stay toward
the bottom of the paper.
Relationships and Biodiversity
 What

is an indicator?
Relationships and Biodiversity
 What

is an indicator?
They are used to detect certain
substances.
In the lab, we used a white powder to
test for “Enzyme M”.
Relationships and Biodiversity
 Gel

Electrophoresis:
 Used for: ___________________
The top indicates the DNA sources.

This side
indicates how
many base pairs
are in the DNA
fragments.
Relationships and Biodiversity
 Gel

Electrophoresis:
 Used for: Comparing DNA samples
The top indicates the DNA sources.

This side
indicates how
many base pairs
are in the DNA
fragments.
Relationships and Biodiversity
 Gel

Electrophoresis:

The DNA fragments
are sorted based
on __________
Relationships and Biodiversity
 Gel

Electrophoresis:

The DNA fragments
are sorted based
on SIZE.
An electrical current
pulls the smaller
fragments through
the “gel” faster than
the larger
fragments.
Relationships and Biodiversity
 Gel

Electrophoresis:

The original samples of DNA are
“cut” using RESTRICTION
ENZYMES.
In the lab, you simulated this by
using scissors to cut paper DNA.
Relationships and Biodiversity
 mRNA

codons code for __________
 Example:
– DNA code: CGA
– mRNA code: ________
– Amino acid chain: (on next page)
 ______________,

______________,
___________________.
Relationships and Biodiversity
 What

causes extinction and loss of
biodiversity?
Relationships and Biodiversity
 What

causes extinction and loss of
biodiversity?
 Change

in environment

 Disease
 Human

activities (pollution,
deforestation)
 Overuse of resources (competion)
Relationships and Biodiversity
 Loss

of biodiversity may be a
warning of what?
Relationships and Biodiversity
 Loss

of biodiversity may be a
warning of what?
 Extinction
 Unstable

environment
Relationships and Biodiversity
 Benefits

of biodiversity:
Relationships and Biodiversity
 Benefits
 More

of biodiversity:

food sources for consumers
 Less chance of extinction
 Medicinal resources
 More stable ecosystem
Diffusion Through a Membrane
 What

causes the process of
diffusion?
Diffusion Through a Membrane
 What

causes the process of
diffusion?

The particles (molecules) move
down a concentration gradient from
high concentration to low
concentration.
This happens because as molecules
bump into each other, they are
forced to spread out to where there
aren’t as many molecules to bump
into.
Diffusion Through a Membrane
 If

certain molecules encounter
membranes with pores, what can
happen?
Diffusion Through a Membrane
 If

certain molecules encounter
membranes with pores, what can
If the molecules (particles) are small
happen?
enough, they will pass through the
pores in the membrane.

If they are too big, they cannot pass
through the pore.

SELECTIVE
PERMEABILITY. Cell
This is called

membranes are selectively
permeable.
Diffusion Through a Membrane
 What

was different between your
model and a real cell?
Diffusion Through a Membrane
 What

was different between your
model and a real cell?

Not living

No organelles
No proteins
Cannot do active
transport (only
passive = diffusion)

Living

BOTH:
Have a
selectively
permeable
membrane
Can do
passive
transport

Has organelles
Has proteins
Does both active and
passive transport
Diffusion Through a Membrane
 What

lab?

indicators were used in the
Diffusion Through a Membrane
 What

lab?

indicators were used in the

IODINE was used to test
for the presence of
STARCH. Color changes
from amber to
blue/black.

BENEDICT’S
SOLUTION (blue) tests
for GLUCOSE. After
heating, it turns
orange if glucose is
present.
Diffusion Through a Membrane
 We

used test tubes with just water
and Benedict’s and another with just
water and iodine. Why?
Diffusion Through a Membrane
 We

used test tubes with just water
and Benedict’s and another with just
water and iodine. Why?
These two test tubes
served as negative
controls. This showed us
what a negative test
would look like.
Diffusion Through a Membrane
 We

also used test tubes with iodine
and starch, and another with
Bendict’s and glucose. Why?
Diffusion Through a Membrane
 We

also used test tubes with iodine
and starch, and another with
Bendict’s and glucose. Why?
These two test tubes
served as positive
controls. They showed
us what a positive test
for starch and glucose
looked like.
Diffusion Through a Membrane
 What

will happen in this experiment?
Diffusion Through a Membrane
 What

will happen in this experiment,
The iodine is small
and why?

enough to fit through
the membrane. It
diffuses into the “cell”
and turns the starch
inside a blue/black
color. The starch is
too large to diffuse
through the selectively
permeable membrane,
so it cannot diffuse out
into the surrounding
solution.
Diffusion Through a Membrane
 Glucose

is in the cell below. What
will happen, and how do you know?
Diffusion Through a Membrane
 Glucose

is in the cell below. What
will happen, and how do you know?

When we test the outside
solution for glucose using
Benedict’s (after heating it), the
solution turns orange. This
indicates that the glucose
diffused out of the cell into the
surrounding solution. The
glucose was small enough to fit
through the selectively
permeable membrane.
Diffusion Through a Membrane
 Osmosis
 Explain

the process that is occurring
in the diagram in the link.
Diffusion Through a Membrane
 Osmosis
 Explain

the process that is occurring
in the diagram in the link.
– The water molecules move from an
area of high concentration of water
(lower solute) to an area of lower
concentration of water (higher
solute).
Diffusion Through a Membrane
 Which

way will diffusion (osmosis)
occur? (Percents indicate
concentration of water.)

97%

99%
Diffusion Through a Membrane
 Which

way will diffusion (osmosis)
occur? (Percents indicate
concentration of water.)

97%

99%
Diffusion Through a Membrane
 Which

way will diffusion of water
(osmosis) occur? (Percents indicate
concentration of solute.)

3%

1%
Diffusion Through a Membrane
 Which

way will diffusion of water
(osmosis) occur? (Percents indicate
concentration of solute.)

3%

1%
Diffusion Through a Membrane
 What

is happening here?
Diffusion Through a Membrane
 What

is happening here?
When salt water is placed on cells,
the water diffuses out of the cells’
cytoplasm into the salt solution.
(Water moves from a higher
concentration – inside the cell – to
a lower concentration – outside
the cell.)
If fresh water is placed on the
cells again, the water will flow
back into the cells (high to low
concentration).
Diffusion Through a Membrane
 Why

didn’t the cell walls shrink with
the cytoplasm?
Diffusion Through a Membrane
 Why

didn’t the cell walls shrink with
the cytoplasm?
– Cell walls are rigid – they provide
support for plant cells.
Diffusion Through a Membrane
 Does

this organism live in fresh
water or salt water? Explain how
you can tell.
Diffusion Through a Membrane
 Does

this organism live in fresh
water or salt water? Explain how
you can tell. Fresh water: The organism contains a
contractile vacuole. Contractile
vacuoles serve to pump out excess
water from the interior of the cell.

If the organism needs to pump out
water, it must have a lower
concentration of water on its inside
than outside (water keeps diffusing
into it). This means that the water
outside has fewer solutes. The water
must be fresh.
Making Connections
 What

lab?

are the two purposes of this

Making

connections between
data and conclusions
Designing a controlled
experiment
Making Connections
 Why

is it more accurate to take two
or three pulse readings and average
them?
Making Connections
 Why

is it more accurate to take two
or three pulse readings and average
them?
Cancel

out errors
The data may vary – this makes
it more reliable
Making Connections
 Why

were the class results averaged
and used?
Making Connections
 Why

were the class results averaged
and used?
More trials means more accurate
data
More subjects means more
accurate data, too.
Making Connections
What

is muscle fatigue?
Making Connections
What

is muscle fatigue?

The “tiring” of the
muscles through
overexertion.
Making Connections
What

waste products cause
muscle fatigue?
Making Connections
What

waste products cause
muscle fatigue?
Mitochondria
ATP

O2 + glucose

CO2 + H2O
Capillary
Making Connections
What

waste products cause
muscle fatigue? CO2, Lactic
Mitochondria
Acid
ATP

Or, Lactic acid
O2 + glucose

CO2 + H2O
Capillary
Making Connections
 Why

does muscle fatigue happen?
Making Connections


Why does muscle fatigue happen?
 The

more the muscle is used, the more
waste products are produced. If the
blood isn’t circulating fast enough to
carry away the wastes, they build up.
As a result, the heart pumps faster and
breathing increases to circulate the
wastes out to the lungs faster. (This
also brings O2 and glucose to the cells
faster to be used for making ATP.)

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Beaks of Finches Lab Review

  • 1. Living Environment Part D (Required Labs) Review
  • 2. Beaks of Finches  14 Species of Darwin’s finches – Beaks vary in size and shape – Bodies vary in size and shape
  • 3. Beaks of Finches  Different beak sizes and shapes differ in their efficiency at performing particular tasks.
  • 4. Beaks of Finches  Why do offspring of better-adapted individuals inherit many of their parents’ favorable variations?
  • 5. Beaks of Finches  Why do offspring of better-adapted individuals inherit many of their parents’ favorable variations? – Favorable variations allow parents to survive and then reproduce more. Their offspring then may possess those variations.
  • 6. Beaks of Finches  What are the parts of Darwin’s theory of Natural Selection?
  • 7. Beaks of Finches  What are the parts of Darwin’s theory of Natural Selection? –OVCSR Only (Overproduction) Vince (Variation) Can (Competition) Suck (Survival of the Fittest) Rutabegas (Reproduction)
  • 8. Beaks of Finches  Variation  means ______________. Why do the finches of the Galapagos Islands show so much variation?
  • 9. Beaks of Finches  Variation  means _differences___. Why do the finches of the Galapagos Islands show so much variation? – Each finch species adapted to their different environments.
  • 10. Beaks of Finches  What did the tools represent?
  • 11. Beaks of Finches  What did the tools represent? – The different beak shapes.
  • 12. Beaks of Finches  What did the different seeds represent?
  • 13. Beaks of Finches  What did the different seeds represent?  The different types of food available on the island.
  • 14. Beaks of Finches  Why are the 4 trials completed and then averaged for each of the “feedings”?
  • 15. Beaks of Finches  Why are the 4 trials completed and then averaged for each of the “feedings”? The data is more accurate when the trials are averaged.
  • 16. Beaks of Finches  All of the finches have similarities. Why is this the case?
  • 17. Beaks of Finches  All of the finches have similarities. Why is this the case? – All of the finches share a common ancestor.
  • 18. Relationships and Biodiversity  Botana curus (fictional plant for lab purposes) produces Curol. – This was supposedly used to ________________.
  • 19. Relationships and Biodiversity  Botana curus (fictional plant for lab purposes) produces Curol. – This was supposedly used to cure cancer.
  • 20. Relationships and Biodiversity The plants used in the lab, and what they were modeled after… maybe? This is the Pacific Yew. It contains “Taxol” – used to treat cancer. This is what was used in the lab, though.
  • 21. Relationships and Biodiversity  Why was it necessary to find an alternative to Botana Curus?  Story of Taxol
  • 22. Relationships and Biodiversity  Why was it necessary to find an alternative to Botana Curus? http://www.phcog.org/Taxus/Taxus_Web.html  In the lab, the plant was endangered and was in short supply.  (In reality, the Pacific Yew only produces one dose of drug per tree! Scientists wanted to find an alternative!)
  • 23. Relationships and Biodiversity  Chromatography: Sorts molecules based on ____
  • 24. Relationships and Biodiversity  Chromatography: Sorts molecules based on SIZE. The smaller molecules move faster up the paper. Larger ones move slower and stay toward the bottom of the paper.
  • 25. Relationships and Biodiversity  What is an indicator?
  • 26. Relationships and Biodiversity  What is an indicator? They are used to detect certain substances. In the lab, we used a white powder to test for “Enzyme M”.
  • 27. Relationships and Biodiversity  Gel Electrophoresis:  Used for: ___________________ The top indicates the DNA sources. This side indicates how many base pairs are in the DNA fragments.
  • 28. Relationships and Biodiversity  Gel Electrophoresis:  Used for: Comparing DNA samples The top indicates the DNA sources. This side indicates how many base pairs are in the DNA fragments.
  • 29. Relationships and Biodiversity  Gel Electrophoresis: The DNA fragments are sorted based on __________
  • 30. Relationships and Biodiversity  Gel Electrophoresis: The DNA fragments are sorted based on SIZE. An electrical current pulls the smaller fragments through the “gel” faster than the larger fragments.
  • 31. Relationships and Biodiversity  Gel Electrophoresis: The original samples of DNA are “cut” using RESTRICTION ENZYMES. In the lab, you simulated this by using scissors to cut paper DNA.
  • 32. Relationships and Biodiversity  mRNA codons code for __________  Example: – DNA code: CGA – mRNA code: ________ – Amino acid chain: (on next page)  ______________, ______________, ___________________.
  • 33.
  • 34. Relationships and Biodiversity  What causes extinction and loss of biodiversity?
  • 35. Relationships and Biodiversity  What causes extinction and loss of biodiversity?  Change in environment  Disease  Human activities (pollution, deforestation)  Overuse of resources (competion)
  • 36. Relationships and Biodiversity  Loss of biodiversity may be a warning of what?
  • 37. Relationships and Biodiversity  Loss of biodiversity may be a warning of what?  Extinction  Unstable environment
  • 38. Relationships and Biodiversity  Benefits of biodiversity:
  • 39. Relationships and Biodiversity  Benefits  More of biodiversity: food sources for consumers  Less chance of extinction  Medicinal resources  More stable ecosystem
  • 40. Diffusion Through a Membrane  What causes the process of diffusion?
  • 41. Diffusion Through a Membrane  What causes the process of diffusion? The particles (molecules) move down a concentration gradient from high concentration to low concentration. This happens because as molecules bump into each other, they are forced to spread out to where there aren’t as many molecules to bump into.
  • 42. Diffusion Through a Membrane  If certain molecules encounter membranes with pores, what can happen?
  • 43. Diffusion Through a Membrane  If certain molecules encounter membranes with pores, what can If the molecules (particles) are small happen? enough, they will pass through the pores in the membrane. If they are too big, they cannot pass through the pore. SELECTIVE PERMEABILITY. Cell This is called membranes are selectively permeable.
  • 44. Diffusion Through a Membrane  What was different between your model and a real cell?
  • 45. Diffusion Through a Membrane  What was different between your model and a real cell? Not living No organelles No proteins Cannot do active transport (only passive = diffusion) Living BOTH: Have a selectively permeable membrane Can do passive transport Has organelles Has proteins Does both active and passive transport
  • 46. Diffusion Through a Membrane  What lab? indicators were used in the
  • 47. Diffusion Through a Membrane  What lab? indicators were used in the IODINE was used to test for the presence of STARCH. Color changes from amber to blue/black. BENEDICT’S SOLUTION (blue) tests for GLUCOSE. After heating, it turns orange if glucose is present.
  • 48. Diffusion Through a Membrane  We used test tubes with just water and Benedict’s and another with just water and iodine. Why?
  • 49. Diffusion Through a Membrane  We used test tubes with just water and Benedict’s and another with just water and iodine. Why? These two test tubes served as negative controls. This showed us what a negative test would look like.
  • 50. Diffusion Through a Membrane  We also used test tubes with iodine and starch, and another with Bendict’s and glucose. Why?
  • 51. Diffusion Through a Membrane  We also used test tubes with iodine and starch, and another with Bendict’s and glucose. Why? These two test tubes served as positive controls. They showed us what a positive test for starch and glucose looked like.
  • 52. Diffusion Through a Membrane  What will happen in this experiment?
  • 53. Diffusion Through a Membrane  What will happen in this experiment, The iodine is small and why? enough to fit through the membrane. It diffuses into the “cell” and turns the starch inside a blue/black color. The starch is too large to diffuse through the selectively permeable membrane, so it cannot diffuse out into the surrounding solution.
  • 54. Diffusion Through a Membrane  Glucose is in the cell below. What will happen, and how do you know?
  • 55. Diffusion Through a Membrane  Glucose is in the cell below. What will happen, and how do you know? When we test the outside solution for glucose using Benedict’s (after heating it), the solution turns orange. This indicates that the glucose diffused out of the cell into the surrounding solution. The glucose was small enough to fit through the selectively permeable membrane.
  • 56. Diffusion Through a Membrane  Osmosis  Explain the process that is occurring in the diagram in the link.
  • 57. Diffusion Through a Membrane  Osmosis  Explain the process that is occurring in the diagram in the link. – The water molecules move from an area of high concentration of water (lower solute) to an area of lower concentration of water (higher solute).
  • 58. Diffusion Through a Membrane  Which way will diffusion (osmosis) occur? (Percents indicate concentration of water.) 97% 99%
  • 59. Diffusion Through a Membrane  Which way will diffusion (osmosis) occur? (Percents indicate concentration of water.) 97% 99%
  • 60. Diffusion Through a Membrane  Which way will diffusion of water (osmosis) occur? (Percents indicate concentration of solute.) 3% 1%
  • 61. Diffusion Through a Membrane  Which way will diffusion of water (osmosis) occur? (Percents indicate concentration of solute.) 3% 1%
  • 62. Diffusion Through a Membrane  What is happening here?
  • 63. Diffusion Through a Membrane  What is happening here? When salt water is placed on cells, the water diffuses out of the cells’ cytoplasm into the salt solution. (Water moves from a higher concentration – inside the cell – to a lower concentration – outside the cell.) If fresh water is placed on the cells again, the water will flow back into the cells (high to low concentration).
  • 64. Diffusion Through a Membrane  Why didn’t the cell walls shrink with the cytoplasm?
  • 65. Diffusion Through a Membrane  Why didn’t the cell walls shrink with the cytoplasm? – Cell walls are rigid – they provide support for plant cells.
  • 66. Diffusion Through a Membrane  Does this organism live in fresh water or salt water? Explain how you can tell.
  • 67. Diffusion Through a Membrane  Does this organism live in fresh water or salt water? Explain how you can tell. Fresh water: The organism contains a contractile vacuole. Contractile vacuoles serve to pump out excess water from the interior of the cell. If the organism needs to pump out water, it must have a lower concentration of water on its inside than outside (water keeps diffusing into it). This means that the water outside has fewer solutes. The water must be fresh.
  • 68. Making Connections  What lab? are the two purposes of this Making connections between data and conclusions Designing a controlled experiment
  • 69. Making Connections  Why is it more accurate to take two or three pulse readings and average them?
  • 70. Making Connections  Why is it more accurate to take two or three pulse readings and average them? Cancel out errors The data may vary – this makes it more reliable
  • 71. Making Connections  Why were the class results averaged and used?
  • 72. Making Connections  Why were the class results averaged and used? More trials means more accurate data More subjects means more accurate data, too.
  • 74. Making Connections What is muscle fatigue? The “tiring” of the muscles through overexertion.
  • 76. Making Connections What waste products cause muscle fatigue? Mitochondria ATP O2 + glucose CO2 + H2O Capillary
  • 77. Making Connections What waste products cause muscle fatigue? CO2, Lactic Mitochondria Acid ATP Or, Lactic acid O2 + glucose CO2 + H2O Capillary
  • 78. Making Connections  Why does muscle fatigue happen?
  • 79. Making Connections  Why does muscle fatigue happen?  The more the muscle is used, the more waste products are produced. If the blood isn’t circulating fast enough to carry away the wastes, they build up. As a result, the heart pumps faster and breathing increases to circulate the wastes out to the lungs faster. (This also brings O2 and glucose to the cells faster to be used for making ATP.)