The Lecture tells about the basic precautions needed to be taken in molecular biology and microbiology lab.

1. Biosafety In Molecular
Biology
LEC 6

2. Molecular Biology Lab
• Advanced understanding of biological processes
• Several biohazards needing containment
• Principles of containment include safe methods, facilities, equipment.
• Biosafety will ensure any potential exposure with hazardous
substances.

3. Laboratory Practice and Education
• Workers must be trained and educated to deal the potential hazards.
• Proficient to use lab equipment, chemicals and infectious substances.

4. Work and Risk Assessment
• Identification of potential risks
• Classification of risks in risk groups.
• Compliance with standard microbiological practices.
• Prohibition of mouth pipetting.
• Washing and sanitizing hands.
• Safe handling of sharps according to practice control measures.
• Decontamination of surface and equipment.
• Waste decontamination and disposal.

5. • Electrocution
• Eyewash station
• Display and dispatch biosafety manual
• Biosafety labels and signs
• Pest management
• Material safety datasheets for chemicals
• Doors for access control
• Safety cabinets

6. • PPEs
• Biosafety cabinets
• Vaccination of lab workers
• Class 1 Cabinets
• Inward airflow protects workers
• Exhaust to outside with HEPA filters

7. • Class 2 Cabinets
• Protection of worker, product, environment
• Used with aerosol transmissible organisms, chemicals
• Used for microorganisms and tissue culture
• Class 3 Cabinets
• Totally enclosed
• Ventilated
• Air-tight
• Usually for BSL 3 and 4

8. • Transportation of samples through authorized commercial entities
• Precautions on Primate cells and Cell Lines