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Endocytosis

       By
Dr. Fatima Riaz
Transport across Cell Membrane
• Diffusion is the process of cells moving from
  areas of high concentration to areas of low
  concentration.

  – This random movement causes the molecules to
    become evenly spread out.
Diffusion
• Cells use diffusion to get substances into and
  out of the cell.
  – Example: During photosynthesis, oxygen is
    produced inside the cell. When this happens the
    concentration of oxygen becomes higher inside
    the cell than outside and oxygen diffuses out of
    the cell.
• Osmosis is the diffusion of water through a
  membrane.
  – Water moves into an area with low concentrations
    and out of areas with high concentrations.


• Both diffusion and osmosis are forms of
  passive transport (they require no energy)
Transport across Cell Membrane:
             Active Transport

• materials move from low concentration to
  high concentration. requires energy!
Endocytosis
– moving a particle into the cell
   • Phagocytosis
   • Pinocytosis



– Exocytosis: moving a particle from inside the cell
  to outside.
• PINOCYTOSIS
Ingestion of minute particles e.g protein
molecules

• PHAGOCYTOSIS
Ingestion of large particles, such as bacteria
, whole cells or portions of degenerated tissue.
Mechanism of pinocytosis
Movement thru the Cell Membrane: Active
              Transport




 Endocytosis              Exocytosis
Cell Locomotion

• Amoeboid movement
• Ciliary movement
Ameboid movement
• Movement of an entire cell in relation to its
  surroundings
• Involves pseudopodium and ATP
• Mechanism
   – formation of new cell membrane & exocytosis at one
     end
   – Attachment of pseudopodium to tissues
      • Receptor proteins
   – Absorption of the membrane & endocytosis in mid &
     rear portions
   – Detachment of receptor proteins
Ameboid movement
Ameboid movement
• Cells that exhibit ameboid motion
  – WBC
  – Fibroblasts
  – Embryonic cells
• Control of ameboid motion
  – Chemotaxis
     • Positive
     • Negative
Cilia and Ciliary movements
• Whiplike movement of cilia on the surfaces of
  cells
• A sharp pointed hair projecting 2-
  4micrometers from the surface of the cell.
Structure of a cilium
• Covered by an outcropping of cell membrane
• Supported by 11 microtubules, 9 double
  tubules at the periphery and 2 single tubules
  down the center.
• Each cilium is an outgrowth of a structure that
  lies immediately beneath the cell
  membrane, called basal body of the cilium
• All the tubules are linked to one another by a
  complex of protein cross linkages, together
  called the axoneme.
• Multiple protein arms composed of the
  protein dynein (having ATPase activity) project
  outward from each double tubule
• ATP and appropriate magnesium and calcium
  concentration required.
Mechanism of ciliary movement
• Sudden fast forward whip like stroke
• Slow backward stroke
• An effective way of pushing the fluid in the
  direction of forward stroke.
• CELLS EXHIBITING CILIARY MOVEMENT

• 1- Respiratory airways
• 2- Uterine tubes of the female reproductive
      tract.
Q&A

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Cell locomotion

  • 1. Endocytosis By Dr. Fatima Riaz
  • 2. Transport across Cell Membrane • Diffusion is the process of cells moving from areas of high concentration to areas of low concentration. – This random movement causes the molecules to become evenly spread out.
  • 3. Diffusion • Cells use diffusion to get substances into and out of the cell. – Example: During photosynthesis, oxygen is produced inside the cell. When this happens the concentration of oxygen becomes higher inside the cell than outside and oxygen diffuses out of the cell.
  • 4. • Osmosis is the diffusion of water through a membrane. – Water moves into an area with low concentrations and out of areas with high concentrations. • Both diffusion and osmosis are forms of passive transport (they require no energy)
  • 5. Transport across Cell Membrane: Active Transport • materials move from low concentration to high concentration. requires energy!
  • 6. Endocytosis – moving a particle into the cell • Phagocytosis • Pinocytosis – Exocytosis: moving a particle from inside the cell to outside.
  • 7. • PINOCYTOSIS Ingestion of minute particles e.g protein molecules • PHAGOCYTOSIS Ingestion of large particles, such as bacteria , whole cells or portions of degenerated tissue.
  • 9.
  • 10. Movement thru the Cell Membrane: Active Transport Endocytosis Exocytosis
  • 11. Cell Locomotion • Amoeboid movement • Ciliary movement
  • 12. Ameboid movement • Movement of an entire cell in relation to its surroundings • Involves pseudopodium and ATP • Mechanism – formation of new cell membrane & exocytosis at one end – Attachment of pseudopodium to tissues • Receptor proteins – Absorption of the membrane & endocytosis in mid & rear portions – Detachment of receptor proteins
  • 14.
  • 15. Ameboid movement • Cells that exhibit ameboid motion – WBC – Fibroblasts – Embryonic cells • Control of ameboid motion – Chemotaxis • Positive • Negative
  • 16.
  • 17. Cilia and Ciliary movements • Whiplike movement of cilia on the surfaces of cells • A sharp pointed hair projecting 2- 4micrometers from the surface of the cell.
  • 18. Structure of a cilium • Covered by an outcropping of cell membrane • Supported by 11 microtubules, 9 double tubules at the periphery and 2 single tubules down the center. • Each cilium is an outgrowth of a structure that lies immediately beneath the cell membrane, called basal body of the cilium
  • 19.
  • 20. • All the tubules are linked to one another by a complex of protein cross linkages, together called the axoneme. • Multiple protein arms composed of the protein dynein (having ATPase activity) project outward from each double tubule • ATP and appropriate magnesium and calcium concentration required.
  • 21. Mechanism of ciliary movement • Sudden fast forward whip like stroke • Slow backward stroke • An effective way of pushing the fluid in the direction of forward stroke.
  • 22.
  • 23. • CELLS EXHIBITING CILIARY MOVEMENT • 1- Respiratory airways • 2- Uterine tubes of the female reproductive tract.
  • 24. Q&A