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ACEBB Seminar Series Winkles and the origin of species Professor Roger Butlin
Winkles and the origin of species Roger Butlin The University of Sheffield
Ernst Haeckel 1866 Ernst Haeckel 1906
Barton et al. 2007
Species 1 Space Species 2 Time
Species 1 Species 2
Species 1 Species 2 Chorthippusparallelus
Species 1 Bombinavariegata Species 2 Bombinabombina
Drosophila simulans Species 1 Species 2 Jones lab
Species 1 Wu 2001 Species 2
Species 1 What initiates the evolution of reproductive isolation? What completes the evolution of reproductive isolation? What drives the increase in reproductive isolation? Species 2
Butlin et al. 2008
Littorinasaxatilis ‘H’ ‘M’ Thornwick Bay
Independentgradients in Spain and Sweden E (exposed) S (sheltered) Kerstin Johannesson, Göteborg University SU RB Emilio Rolan-AlvarezUniversity of Vigo LOWER MID UPPER Roger Butlin
Scale dependent!
Sympatric and parallel or allopatric and interdependent? Quesada et al. 2007Evolution
Old Peak H Thornwick Bay H Filey H 87 99 Robin Hood’s Bay 2 90 Thornwick Bay M 76 Robin Hood’s Bay 1 88 Old Peak M Filey M 0.01 290 AFLP loci
Wilding et al. 2001
Old Peak H Thornwick Bay M Filey H Thornwick Bay H Filey H Filey M 87 Old Peak H 73 99 99 67 Thornwick Bay H 99 Robin Hood’s Bay 2 81 Robin Hood’s       Bay 2 90 Thornwick Bay M 76 Robin Hood’s Bay 1 88 Robin Hood’s Bay 1 Old Peak M Old Peak M 0.01 Filey M 0.01 All loci                      15 ‘selected’ loci removed
Sympatric and parallel or allopatric and interdependent? All SS have haplotype 1regardless of locality! Petri Kempainnen thesis
BAC library Not differentiated, control (A30) Weakly differentiated (A37) Differentiated (E12) Most differentiated (E10) ‘H’ ‘M’ Littorinasaxatilis Wilding et al 2001 Grahame et al 2006
Predicted gene AFLP Region sequenced A B C D
5.3   *   3.0     4.3     *  0    11.8    6.9   *    6.2        4.2  *  6.0   kb FST=0.00 FST=0.12 FST=0.32 FST=0.54
Similar to tetraspanin 3A of Drosophila Conserved protein of unknown function AFLP E10L0 E10L1 120kb 130kb 140kb
Pea aphid host races on clover and alfalfaVia and West (2008)
454 – based genome scan - 15 individuals / sample - RNA extraction (whole body) H M Juan Galindo
Divergent SNPs
2454 SNPs in 572 contigs 510 SNPs in 197 contigs Outlier (P<0.05 by simulation with ‘Winkle454’) Significant after SGoF correction (http://webs.uvigo.es/acraaj/SGoF.htm) Significant after Benjamini-Hochberg correction *
[object Object]
 Transposable elements?
 Some interesting proteins…Shell formation Lithostatine Mucin Dermatopontin Muscle physiology Myosin Titin Energetic metabolism Argininekinase NADH dehydrogenase

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Winkles and the origin of species

  • 1. ACEBB Seminar Series Winkles and the origin of species Professor Roger Butlin
  • 2.
  • 3.
  • 4.
  • 5. Winkles and the origin of species Roger Butlin The University of Sheffield
  • 6.
  • 7. Ernst Haeckel 1866 Ernst Haeckel 1906
  • 9.
  • 10. Species 1 Space Species 2 Time
  • 12. Species 1 Species 2 Chorthippusparallelus
  • 13. Species 1 Bombinavariegata Species 2 Bombinabombina
  • 14. Drosophila simulans Species 1 Species 2 Jones lab
  • 15. Species 1 Wu 2001 Species 2
  • 16. Species 1 What initiates the evolution of reproductive isolation? What completes the evolution of reproductive isolation? What drives the increase in reproductive isolation? Species 2
  • 19. Independentgradients in Spain and Sweden E (exposed) S (sheltered) Kerstin Johannesson, Göteborg University SU RB Emilio Rolan-AlvarezUniversity of Vigo LOWER MID UPPER Roger Butlin
  • 21. Sympatric and parallel or allopatric and interdependent? Quesada et al. 2007Evolution
  • 22. Old Peak H Thornwick Bay H Filey H 87 99 Robin Hood’s Bay 2 90 Thornwick Bay M 76 Robin Hood’s Bay 1 88 Old Peak M Filey M 0.01 290 AFLP loci
  • 24. Old Peak H Thornwick Bay M Filey H Thornwick Bay H Filey H Filey M 87 Old Peak H 73 99 99 67 Thornwick Bay H 99 Robin Hood’s Bay 2 81 Robin Hood’s Bay 2 90 Thornwick Bay M 76 Robin Hood’s Bay 1 88 Robin Hood’s Bay 1 Old Peak M Old Peak M 0.01 Filey M 0.01 All loci 15 ‘selected’ loci removed
  • 25. Sympatric and parallel or allopatric and interdependent? All SS have haplotype 1regardless of locality! Petri Kempainnen thesis
  • 26. BAC library Not differentiated, control (A30) Weakly differentiated (A37) Differentiated (E12) Most differentiated (E10) ‘H’ ‘M’ Littorinasaxatilis Wilding et al 2001 Grahame et al 2006
  • 27. Predicted gene AFLP Region sequenced A B C D
  • 28. 5.3 * 3.0 4.3 * 0 11.8 6.9 * 6.2 4.2 * 6.0 kb FST=0.00 FST=0.12 FST=0.32 FST=0.54
  • 29. Similar to tetraspanin 3A of Drosophila Conserved protein of unknown function AFLP E10L0 E10L1 120kb 130kb 140kb
  • 30. Pea aphid host races on clover and alfalfaVia and West (2008)
  • 31. 454 – based genome scan - 15 individuals / sample - RNA extraction (whole body) H M Juan Galindo
  • 33. 2454 SNPs in 572 contigs 510 SNPs in 197 contigs Outlier (P<0.05 by simulation with ‘Winkle454’) Significant after SGoF correction (http://webs.uvigo.es/acraaj/SGoF.htm) Significant after Benjamini-Hochberg correction *
  • 34.
  • 35.
  • 37. Some interesting proteins…Shell formation Lithostatine Mucin Dermatopontin Muscle physiology Myosin Titin Energetic metabolism Argininekinase NADH dehydrogenase
  • 38.
  • 39. Thanks! John Grahame, Craig Wilding, Henry Wood and Juan Galindo – Littorina NERC and BBSRC – cash This sketch is most imperfect; but in so short a space I cannot make it better. Your imagination must fill up very wide blanks. Darwin, 1858
  • 40. Next seminar A system for establishing TERN in Australia Associate Professor Jean-Marc Hero