ACEBB Seminar Series<br />Winkles and the origin of species<br />Professor Roger Butlin<br />
Winkles and the origin of species<br />Roger Butlin<br />The University of Sheffield<br />
Ernst Haeckel 1866<br />Ernst Haeckel 1906<br />
Barton et al. 2007<br />
Species 1<br />Space<br />Species 2<br />Time<br />
Species 1<br />Species 2<br />
Species 1<br />Species 2<br />Chorthippusparallelus<br />
Species 1<br />Bombinavariegata<br />Species 2<br />Bombinabombina<br />
Drosophila simulans<br />Species 1<br />Species 2<br />Jones lab<br />
Species 1<br />Wu 2001<br />Species 2<br />
Species 1<br />What initiates the evolution of reproductive isolation?<br />What completes the evolution of reproductive i...
Butlin et al. 2008<br />
Littorinasaxatilis<br />‘H’<br />‘M’<br />Thornwick Bay<br />
Independentgradients in Spain and Sweden<br />E (exposed)<br />S (sheltered)<br />Kerstin Johannesson, Göteborg University...
Scale dependent!<br />
Sympatric and parallel or allopatric and interdependent?<br />Quesada et al. 2007Evolution<br />
Old Peak H<br />Thornwick Bay H<br />Filey H<br />87<br />99<br />Robin Hood’s Bay 2<br />90<br />Thornwick Bay M<br />76<...
Wilding et al. 2001<br />
Old Peak H<br />Thornwick Bay M<br />Filey H<br />Thornwick Bay H<br />Filey H<br />Filey M<br />87<br />Old Peak H<br />7...
Sympatric and parallel or allopatric and interdependent?<br />All SS have haplotype 1regardless of locality!<br />Petri Ke...
BAC library<br />Not differentiated, control (A30)<br />Weakly differentiated (A37)<br />Differentiated (E12)<br />Most di...
Predicted gene<br />AFLP<br />Region sequenced<br />A<br />B<br />C<br />D<br />
5.3   *   3.0     4.3     *  0    11.8    6.9   *    6.2        4.2  *  6.0   kb<br />FST=0.00<br />FST=0.12<br />FST=0.32...
Similar to tetraspanin 3A of Drosophila<br />Conserved protein of unknown function<br />AFLP<br />E10L0<br />E10L1<br />12...
Pea aphid host races on clover and alfalfaVia and West (2008)<br />
454 – based genome scan<br />- 15 individuals / sample<br />- RNA extraction (whole body)<br />H<br />M<br />Juan Galindo<...
Divergent SNPs<br />
2454 SNPs in 572 contigs<br />510 SNPs in 197 contigs<br />Outlier (P&lt;0.05 by simulation with ‘Winkle454’)<br />Signifi...
<ul><li> Food and parasites!
 Transposable elements?
 Some interesting proteins…</li></ul>Shell formation<br />Lithostatine<br />Mucin<br />Dermatopontin<br />Muscle physiolog...
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Winkles and the origin of species

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Professor Roger Butlin from The University of Sheffield (UK) presents the first seminar in the ACEBB Science Seminar Series entitled "Winkles and the origin of species".

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Winkles and the origin of species

  1. ACEBB Seminar Series<br />Winkles and the origin of species<br />Professor Roger Butlin<br />
  2. Winkles and the origin of species<br />Roger Butlin<br />The University of Sheffield<br />
  3. Ernst Haeckel 1866<br />Ernst Haeckel 1906<br />
  4. Barton et al. 2007<br />
  5. Species 1<br />Space<br />Species 2<br />Time<br />
  6. Species 1<br />Species 2<br />
  7. Species 1<br />Species 2<br />Chorthippusparallelus<br />
  8. Species 1<br />Bombinavariegata<br />Species 2<br />Bombinabombina<br />
  9. Drosophila simulans<br />Species 1<br />Species 2<br />Jones lab<br />
  10. Species 1<br />Wu 2001<br />Species 2<br />
  11. Species 1<br />What initiates the evolution of reproductive isolation?<br />What completes the evolution of reproductive isolation?<br />What drives the increase in reproductive isolation?<br />Species 2<br />
  12. Butlin et al. 2008<br />
  13. Littorinasaxatilis<br />‘H’<br />‘M’<br />Thornwick Bay<br />
  14. Independentgradients in Spain and Sweden<br />E (exposed)<br />S (sheltered)<br />Kerstin Johannesson, Göteborg University<br />SU<br />RB<br />Emilio Rolan-AlvarezUniversity of Vigo<br />LOWER<br />MID<br />UPPER<br />Roger Butlin<br />
  15. Scale dependent!<br />
  16. Sympatric and parallel or allopatric and interdependent?<br />Quesada et al. 2007Evolution<br />
  17. Old Peak H<br />Thornwick Bay H<br />Filey H<br />87<br />99<br />Robin Hood’s Bay 2<br />90<br />Thornwick Bay M<br />76<br />Robin Hood’s Bay 1<br />88<br />Old Peak M<br />Filey M<br />0.01<br />290 AFLP loci<br />
  18. Wilding et al. 2001<br />
  19. Old Peak H<br />Thornwick Bay M<br />Filey H<br />Thornwick Bay H<br />Filey H<br />Filey M<br />87<br />Old Peak H<br />73<br />99<br />99<br />67<br />Thornwick Bay H<br />99<br />Robin Hood’s Bay 2<br />81<br />Robin Hood’s <br /> Bay 2<br />90<br />Thornwick Bay M<br />76<br />Robin Hood’s Bay 1<br />88<br />Robin Hood’s Bay 1<br />Old Peak M<br />Old Peak M<br />0.01<br />Filey M<br />0.01<br />All loci 15 ‘selected’ loci removed<br />
  20. Sympatric and parallel or allopatric and interdependent?<br />All SS have haplotype 1regardless of locality!<br />Petri Kempainnen thesis<br />
  21. BAC library<br />Not differentiated, control (A30)<br />Weakly differentiated (A37)<br />Differentiated (E12)<br />Most differentiated (E10)<br />‘H’<br />‘M’<br />Littorinasaxatilis<br />Wilding et al 2001<br />Grahame et al 2006<br />
  22. Predicted gene<br />AFLP<br />Region sequenced<br />A<br />B<br />C<br />D<br />
  23. 5.3 * 3.0 4.3 * 0 11.8 6.9 * 6.2 4.2 * 6.0 kb<br />FST=0.00<br />FST=0.12<br />FST=0.32<br />FST=0.54<br />
  24. Similar to tetraspanin 3A of Drosophila<br />Conserved protein of unknown function<br />AFLP<br />E10L0<br />E10L1<br />120kb<br />130kb<br />140kb<br />
  25. Pea aphid host races on clover and alfalfaVia and West (2008)<br />
  26. 454 – based genome scan<br />- 15 individuals / sample<br />- RNA extraction (whole body)<br />H<br />M<br />Juan Galindo<br />
  27. Divergent SNPs<br />
  28. 2454 SNPs in 572 contigs<br />510 SNPs in 197 contigs<br />Outlier (P&lt;0.05 by simulation with ‘Winkle454’)<br />Significant after SGoF correction (http://webs.uvigo.es/acraaj/SGoF.htm)<br />Significant after Benjamini-Hochberg correction<br />*<br />
  29. <ul><li> Food and parasites!
  30. Transposable elements?
  31. Some interesting proteins…</li></ul>Shell formation<br />Lithostatine<br />Mucin<br />Dermatopontin<br />Muscle physiology<br />Myosin<br />Titin<br />Energetic metabolism<br />Argininekinase<br />NADH dehydrogenase<br />
  32. Thanks!<br />John Grahame, Craig Wilding, Henry Wood and Juan Galindo – Littorina<br />NERC and BBSRC – cash<br />This sketch is most imperfect; but in so short a space I cannot make it better. Your imagination must fill up very wide blanks.<br />Darwin, 1858<br />
  33. Next seminar <br />A system for establishing TERN in Australia<br />Associate Professor Jean-Marc Hero<br />

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