OS20 Online 10 December

1. 1EuFMD | Open Session special edition | #OS20se
National Animal and Plant Quarantine Agency / Republic of Korea
VALIDATION OF RECOMBINANT
PROTEIN-BASED ELISA FOR DETECTION
OF ANTIBODIES TO FOOT-AND-MOUTH
DISEASE VIRUS TYPE-O
Eun-Jin Choi (Presenter), YouJin Han, JiHyun Lee, Hyun Mi Pyo,
Doheon Gwon, Wonseok Shin, JaeMyoung Kim and Mi-Young Park

2. 2EuFMD | Open Session special edition | #OS20se
 The ELISA (r-SPCE) kit has been developed and validated with
field sera collected from either vaccinated or non-vaccinated
from non-infected cattle and pig farms, international reference
sera obtained from WRLFMD, Pirbright and sera from
experimentally challenged animals.
 The recombinant protein (rP13C) was derived from
recombinant VP4231 protein of FMDV O/JC/SKR/2014
(O/SEA/Mya98) genes using a baculovirus-expressed system.
Main Idea

3. 3EuFMD | Open Session special edition | #OS20se
 The overall diagnostic specificity of 99.9% with a threshold of 50%
inhibition was identified by analyzing 1,070 cattle and 730 pigs from
FMD-free and non-vaccinated country (Canada, USA).
 Diagnostic sensitivity estimated using 475 known positive animal sera
result of 95.2%.
 International reference sera were correctly detected.
 A high level of serotype-specificity was demonstrated testing
IAEA/FAO standard sera strongly positive against other FMDV
serotypes.
 Also, the CV obtained for evaluating reproducibility and repeatability
estimated ≤10%, demonstrating the excellent stability of the ELISA (r-
SPCE).

4. 4EuFMD | Open Session special edition | #OS20se
 Objectives
- A new recombinant protein-based solid-phase competitive ELISA(r-
SPCE) with monoclonal antibody (Mab) for detection of antibodies to
FMDV type-O and has been developed and validated with field sera
and international reference sera.
 Outcomes
- The recombinant protein-based SPCE developed for the detection of
antibodies to FMDV type-O demonstrated high specificity and
sensitivity. In addition, this assay offers advantages in terms of
repeatability and broader cross-specificity, but also is easy-to use
and applicable to multiple species.

5. eufmdvirtual.com
8, 10, 15, 17 December 2020
EuFMD / Open Session